cyran et al. (2003) cell vol 112 pp. 329-341
DESCRIPTION
Cyran et al. (2003) Cell vol 112 pp. 329-341. V3 : transgène permettant une forte expression constitutive de VRI. per 0. per 0. Cyran et al. (2003) Cell vol 112 pp. 329-341. tK : tyrosine kinase du virus Herpès Non piloté par l’horloge moléculaire. - PowerPoint PPT PresentationTRANSCRIPT
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Gène
Transcription
Inhibition
Traduction
ARNm
Protéine
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PeriodTimelessClockCycleDouble-time
(Per)(Tim)(Clk)(Cyc)(Dbt)
Exprimés de façonrythmique
0 12 24 h
ARNm
Per, Tim
Clk
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Cytoplasme
Per PER
TIM
PER TIMPER TIM
Tim
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Cytoplasme
Per PER
TIM
PER TIMPER TIM
Tim
DBT Protéine Kinase
Dégradation accélérée
PER P
0 12 24 h
ARNm
Protéine
Per, Tim
PER, TIM
DBT
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Tim
Per
E-Box
CACGTG
Per TimCLK CYC
++++
PER TIM
CLK CYC
Per Tim- - - -
PER
TIM
PER TIM
DBT
CLK CYC
CLK
CYC
Per TimCLK CYC
++++
Clock
PER TIMPER
P
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CLK
Activation
Inhibition?
? Exprimé de façonrythmique
Le niveau des ARNm Clock est faible chez des drosophiles mutantes présentantsoit une protéine PER inactive, soit une protéine TIM inactive.
Glossop N.R.J., Lyons LC., Hardin P.E. Vol 286, p. 766-768.Science, 22 Octobre 1999,
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ARNm Clock Drosophiles
Sauvages
Clkjrk
Cyc 0
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ARNm Clock
Sauv. Per01 Per01
Clkjrk Cyc0
Per01
ARNm prélevés à
01 heure
13 heures
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ARNm Clock
Cyc0Clkjrk
Cyc0
Per01
ARNm prélevés à
01 heure
13 heuresSauv.
1 13
LO OO
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TIM
PER TIM
DBT
CLK CYC
CLK
CYC
Per TimCLK CYC
CLK CYC
++++
Clock
PER TIM
PER
PIntermédiaires ?
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Cyran et al. (2003) Cell vol 112 pp. 329-341
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Cyran et al. (2003) Cell vol 112 pp. 329-341
V3: transgène permettant une forte expression constitutive de VRI
per0 per0
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Cyran et al. (2003) Cell vol 112 pp. 329-341
tK: tyrosine kinase du virus HerpèsNon piloté par l’horloge moléculaire
VRI-VP16: Protéine de fusion: répresseur VRI + domaine de recrutement de l’ARN polymérase. Donne un activateur de transcription
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Est rapidement dégradée en présence de lumière
Cryptochromes
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Per, TimClk Clk Clk Clk ClkClk
Per, TimClk Clk Clk Clk Clk
Per, TimCry Cryb CrybCry
Per, Tim
ObscuritéLumière
20
40
60
80
100
120
Ac
tiv
ati
on
re
lati
ve p
ar
Clo
ck
Cellules embryonnaires S2 transfectées
Construction "Gene-reporter": promoteur TIM + Luciférase
Les Co-transfectants sont indiqués sous les histogrammes
Seriani et al. (1999) Science vol 285 pp. 553-556
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Domaine de fixation à l'ADNDomaine
ActivateurCRYPER
PER
TIM
CRYbCRYPER CRYb
TIM
TIM PER
Seriani et al. (1999) Science vol 285 pp. 553-556
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Monarque
Migration d’automne
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Suivi des éclosions
Dosage de l’ARN per
Froy et al. (2003) Science vol 300 pp. 1303-1305
LD
DDLL
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0 12 24
0700-1900
0100-1300
07:00 19:00
Test d’orientation du vol
Froy et al. (2003) Science vol 300 pp. 1303-1305
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6 h(115°)
0700-1900
0100-1300
Lumière constante
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Per/Tim
Clk/Cyc
Sud Sud
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Froy et al. (2003) Science vol 300 pp. 1303-1305
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Horloge PER/TIM chez la Drosophile
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Horloge PER/TIM chez les Mammifères