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54 DAFTAR PUSTAKA 1. Botham KM, Mayes PA. Pengangkutan dan Penyimpanan Lipid. In: Biokimia Harper. 27th ed. Jakarta: EGC; 2012. 2. Prassl R, Laggner P. Lipoprotein Structure and Dynamics : Low Density Lipoprotein Viewed as a Highly Dynamic and Flexible Nanoparticle. Biochem Genet Mol Biol Lipoproteins - Role Heal Dis [Internet]. 2012;320. Available from: http://www.intechopen.com/books/lipoproteins-role-in- health-and-diseases/dyslipoproteinemia-in-chronic-hcv-infection 3. Murtola T, Vuorela TA, Hyvönen MT, Marrink S-J, Karttunen M, Vattulainen I. Low density lipoprotein: structure, dynamics, and interactions of apoB-100 with lipids. Soft Matter [Internet]. 2011;7(18):8135. Available from: http://xlink.rsc.org/?DOI=c1sm05367a 4. Centers for Disease Control and Prevention. Smoking and Tobacco Use; Fact Sheet; Bidis and Kreteks. [cited 2015 Dec 19]; Available from: http://www.cdc.gov/tobacco/data_statistics/fact_sheets/tobacco_industry/bi dis_kreteks/index.htm 5. The Chemicals in Cigarette Smoke & Their Effects [Internet]. 2015 [cited 2015 Dec 12]. Available from: http://www.compoundchem.com/2014/05/01/the-chemicals-in-cigarette- smoke-their-effects/ 6. Seddon JM, Reynolds R, Rosner B. Associations of smoking, body mass index, dietary lutein, and the LIPC gene variant rs10468017 with advanced age-related macular degeneration. Mol Vis [Internet]. 2010 Jan [cited 2015 Dec 6];16:241224. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2994762&tool =pmcentrez&rendertype=abstract 7. Preedy VR. Essential Oils in Food Preservation, Flavor and Safety [Internet]. Elsevier Science; 2015. Available from: https://books.google.co.id/books?id=uNDUBQAAQBAJ 8. Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, et al. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian

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Page 1: DAFTAR PUSTAKA - eprints.undip.ac.ideprints.undip.ac.id/50720/8/Lalita_Khairunnisa_22010112130104_Lap... · DAFTAR PUSTAKA 1. Botham KM, Mayes PA. Pengangkutan dan Penyimpanan Lipid

54

DAFTAR PUSTAKA

1. Botham KM, Mayes PA. Pengangkutan dan Penyimpanan Lipid. In:

Biokimia Harper. 27th ed. Jakarta: EGC; 2012.

2. Prassl R, Laggner P. Lipoprotein Structure and Dynamics : Low Density

Lipoprotein Viewed as a Highly Dynamic and Flexible Nanoparticle.

Biochem Genet Mol Biol Lipoproteins - Role Heal Dis [Internet]. 2012;3–

20. Available from: http://www.intechopen.com/books/lipoproteins-role-in-

health-and-diseases/dyslipoproteinemia-in-chronic-hcv-infection

3. Murtola T, Vuorela TA, Hyvönen MT, Marrink S-J, Karttunen M,

Vattulainen I. Low density lipoprotein: structure, dynamics, and

interactions of apoB-100 with lipids. Soft Matter [Internet].

2011;7(18):8135. Available from: http://xlink.rsc.org/?DOI=c1sm05367a

4. Centers for Disease Control and Prevention. Smoking and Tobacco Use;

Fact Sheet; Bidis and Kreteks. [cited 2015 Dec 19]; Available from:

http://www.cdc.gov/tobacco/data_statistics/fact_sheets/tobacco_industry/bi

dis_kreteks/index.htm

5. The Chemicals in Cigarette Smoke & Their Effects [Internet]. 2015 [cited

2015 Dec 12]. Available from:

http://www.compoundchem.com/2014/05/01/the-chemicals-in-cigarette-

smoke-their-effects/

6. Seddon JM, Reynolds R, Rosner B. Associations of smoking, body mass

index, dietary lutein, and the LIPC gene variant rs10468017 with advanced

age-related macular degeneration. Mol Vis [Internet]. 2010 Jan [cited 2015

Dec 6];16:2412–24. Available from:

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2994762&tool

=pmcentrez&rendertype=abstract

7. Preedy VR. Essential Oils in Food Preservation, Flavor and Safety

[Internet]. Elsevier Science; 2015. Available from:

https://books.google.co.id/books?id=uNDUBQAAQBAJ

8. Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, et al.

A review on therapeutic potential of Nigella sativa: A miracle herb. Asian

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55

Pac J Trop Biomed. 2013;3(5):337–52.

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10. Ahmad S, Beg ZH. Hypolipidemic and antioxidant activities of

thymoquinone and limonene in atherogenic suspension fed rats. Food

Chem [Internet]. 2013;138(2-3):1116–24. Available from:

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11. Tasawar Z, Siraj Z, Ahmad N, Lashari MH. The Effects of Nigella sativa

(Kalonji) on Lipid Profile in Patients with Stable Coronary Artery Disease

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12. Bamosa A, Lebda F, Al Elq A, Al-Sultan A, Kaatabi H. Favorable impact

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39. Peckett AJ, Wright DC, Riddell MC. The effects of glucocorticoids on

adipose tissue lipid metabolism. Metabolism [Internet]. Elsevier Inc.;

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Swim Stressed Mice. 2011;54–60.

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nicotine and cigarette smoke. Trends Endocrinol Metab [Internet]. Elsevier

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LAMPIRAN

LAMPIRAN I – ETHICAL CLEARANCE

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LAMPIRAN II – PERMOHONAN IZIN PENELITIAN

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LL L

LAMPIRAN III – PERSETUJUAN IZIN PENELITIAN

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LAMPIRAN IV – PROSEDUR PEMBUATAN EKSTRAK JINTAN HITAM

(METODE SOXHLETASI)

Alat:

1. Soxhlet komplit

2. Cawan porselin

3. Pipet tetes

4. Beaker glass

5. Gelas ukur

6. Statip

7. Corong

8. Kertas saring

9. Neraca analitik

10. Water bath

11. Kompor listrik

12. Panci

Bahan:

1. Biji jintan

2. Pelarut etanol 70%

3. Akuades

Cara Kerja:

1. Biji jintan dikeringkan, kemudian ditumbuk sampai halus

2. Biji jintan yang telah ditumbuk ditimbang ± 200 gram (sesuai kapasitas

tabung), dibungkus dengan kertas saring dan dibentuk menyerupai tabung

sesuai besarnya diameter tabung tersebut

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3. Kertas saring berisi jintan hitam dimasukkan ke dalam extraction chamber,

kemudian ditambah pelarut 200 ml

4. Labu alas bulat diisi pelarut 500 ml, kemudian dihubungkan dengan

extraction chamber yang telah dipasang pada statip

5. Sisi bawah labu alas bulat dipasang alat pemanas (kompor) dan panci yang

berisi air

6. Kran pada kondensor dipasang selang dan dihubungkan pada air mengalir,

air dibiarkan mengalir selama proses ekstraksi

7. Pemanas (kompor) dinyalakan kurang lebih enam jam

8. Hasil ekstrak dalam labu alas bulat yang masih bercampur dengan pelarut

dan air diambil, dituangkan dalam cawan porselin, lalu diuapkan diatas

water bath dengan suhu maksimal 70° atau diangin-anginkan sampai

pelarut dan airnya hilang dan didapatkan ekstraknya

9. Prosedur ini diulangi beberapa kali hingga didapatkan ekstrak yang

diinginkan.

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67

LAMPIRAN V – HASIL UJI LABORATORIUM

No. Kode

sampel Kadar kolesterol

LDL

presipitan

Kadar LDL = kadar

kolesterol – kadar

LDL

1 N1 60,5 32,1 28,4 mg/dl

2 N2 65 39,2 25,8 mg/dl

3 N3 62,7 35,4 27,3 mg/dl

4 N4 44,4 22,6 21,8 mg/dl

5 N5 58,3 32,8 25,5 mg/dl

6 N6 50,5 28,1 22,4 mg/dl

7 R1 60,8 33,6 27,2 mg/dl

8 R2 53,3 29,2 24,1 mg/dl

9 R3 50 26,8 23,2 mg/dl

10 R4 60,7 34 26,7 mg/dl

11 R5 72,5 39,8 32,7 mg/dl

12 R6 52,1 26,4 25,7 mg/dl

13 J1 53 32,3 20,7 mg/dl

14 J2 54 31,9 22,1 mg/dl

15 J3 55,4 30,2 25,2 mg/dl

16 J4 51,4 25,8 25,6 mg/dl

17 J5 53,7 28,1 25,6 mg/dl

18 J6 55,1 31,6 23,5 mg/dl

19 S1 52,1 29,1 23 mg/dl

20 S2 75 46,7 28,3 mg/dl

21 S3 60,5 33,7 26,8 mg/dl

22 S4 58,7 29,5 29,2 mg/dl

23 S5 62 34,1 27,9 mg/dl

24 S6 56,5 31,2 25,3 mg/dl

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LAMPIRAN VI – HASIL ANALISIS (OUTPUT PROGRAM SPSS)

1. Jumlah sampel tiap kelompok

2. Analisis deskriptif pengaruh pemberian ekstrak jintan hitam terhadap

kadar kolesterol LDL tikus yang terpapar asap rokok

Descriptives

Kelompok Statistic Std. Error

Kadar LDL Kontrol Mean 25.200 1.0724

95% Confidence Interval for Mean

Lower Bound 22.443 Upper Bound 27.957

5% Trimmed Mean 25.211 Median 25.650 Variance 6.900 Std. Deviation 2.6268 Minimum 21.8 Maximum 28.4 Range 6.6 Interquartile Range 5.3 Skewness -.314 .845

Kurtosis -1.552 1.741

Rokok Mean 26.600 1.3687

95% Confidence Interval for Mean

Lower Bound 23.082 Upper Bound 30.118

5% Trimmed Mean 26.450 Median 26.200 Variance 11.240 Std. Deviation 3.3526 Minimum 23.2

Case Processing Summary

Kelompok

Cases

Valid Missing Total

N Percent N Percent N Percent

Kadar LDL Kontrol 6 100.0% 0 0.0% 6 100.0%

Rokok 6 100.0% 0 0.0% 6 100.0%

Rokok + Jintan 6 100.0% 0 0.0% 6 100.0%

Rokok + Simvastatin 6 100.0% 0 0.0% 6 100.0%

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Maximum 32.7 Range 9.5 Interquartile Range 4.7 Skewness 1.366 .845

Kurtosis 2.383 1.741

Rokok + Jintan Mean 23.783 .8372

95% Confidence Interval for Mean

Lower Bound 21.631 Upper Bound 25.935

5% Trimmed Mean 23.854 Median 24.350 Variance 4.206 Std. Deviation 2.0508 Minimum 20.7 Maximum 25.6 Range 4.9 Interquartile Range 3.9 Skewness -.670 .845

Kurtosis -1.334 1.741

Rokok + Simvastatin Mean 26.750 .9291

95% Confidence Interval for Mean

Lower Bound 24.362 Upper Bound 29.138

5% Trimmed Mean 26.822 Median 27.350 Variance 5.179 Std. Deviation 2.2757 Minimum 23.0 Maximum 29.2 Range 6.2 Interquartile Range 3.8 Skewness -.912 .845

Kurtosis .163 1.741

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3. Hasil uji normalitas data pengaruh pemberian ekstrak jintan hitam

terhadap kadar kolesterol LDL tikus yang terpapar asap rokok

Tests of Normality

Kelompok

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

Kadar LDL Kontrol .212 6 .200* .924 6 .533

Rokok .262 6 .200* .889 6 .311

Rokok + Jintan .255 6 .200* .872 6 .236

Rokok + Simvastatin .193 6 .200* .937 6 .635

*. This is a lower bound of the true significance. a. Lilliefors Significance Correction

4. Hasil uji homogenitas varians dan uji parametrik One Way Anova

Oneway

Test of Homogeneity of Variances

Kadar LDL Levene Statistic df1 df2 Sig.

.201 3 20 .895

ANOVA

Kadar LDL

Sum of Squares df Mean Square F Sig.

Between Groups 34.690 3 11.563 1.680 .203 Within Groups 137.623 20 6.881 Total 172.313 23

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LAMPIRAN VII – DOKUMENTASI PENELITIAN

Proses pemaparan asap rokok pada tikus Sprague Dawley

Proses anestesi dan pengambilan darah tikus

Sprague Dawley melalui pleksus retroorbita

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Ekstraksi biji jintan hitam

dengan metode Soxhletasi

Proses pemeriksaan kadar

kolesterol LDL serum tikus

Sprague Dawley

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LAMPIRAN VIII – BIODATA MAHASISWA

Identitas

Nama : Lalita Khairunnisa

NIM : 22010112130104

Tempat/tanggal lahir : Jakarta, 15 Oktober 1994

Jenis kelamin : Perempuan

Alamat : Citra Gran blok I8/3, Jatisampurna, Bekasi

Nomor telepon : 02184311296

Nomor HP : 081319300559

E-mail : [email protected]

Riwayat Pendidikan Formal

1. SD : SD Cindera Mata Bekasi Lulus tahun: 2006

2. SMP : SMP Negeri 1 Cibinong Lulus tahun: 2009

3. SMA : SMA Negeri 3 Bogor Lulus tahun: 2012

4. FK Undip : Masuk tahun: 2012

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Keanggotaan Organisasi

1. Anggota Paduan Suara Mahasiswa (PSM) Universitas Diponegoro tahun

2012

2. Anggota Divisi Eksterna Badan Kelengkapan Reproductive Health

Education Unit (RHEU) FK Undip tahun 2013

3. Anggota Sie. Acara Try Out FK Undip tahun 2013

4. Anggota Sie. Konsumsi Gebyar Ramadhan tahun 2013

5. Reporter Buletin Communication Medical FK Undip tahun 2013-2014

6. Ketua Badan Kelengkapan Reproductive Health Education Unit (RHEU)

FK Undip masa jabatan tahun 2014-2015

7. Asisten Dosen Biokimia FK Undip masa jabatan 2014-2015

8. Anggota Sie. Acara Scientific Fair FK Undip tahun 2014

9. Anggota Paduan Suara Mahasiswa (PSM) Kedokteran Umum Undip tahun

2014-2015

10. Tim Penyusun Badan Semi Otonom (BSO) Jurnalistik FK Undip tahun

2014

11. Anggota Sie. Acara Porseni FK Undip tahun 2014

12. Sekretaris Umum BSO Lembaga Pers Mahasiswa (LPM) Impuls FK Undip

masa jabatan 2015-2016

13. Steering Committee Badan Kelengkapan Reproductive Health Education

Unit (RHEU) FK Undip masa jabatan tahun 2015-2016

14. Asisten Dosen Biokimia FK Undip masa jabatan 2015-2016

15. Anggota Sie. Pengabdian Masyarakat Kemah Bakti FK Undip tahun 2015

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16. Konselor BSO Lembaga Pers Mahasiswa (LPM) Impuls FK Undip masa

jabatan 2016-2017