Data Pengamatan1. Ekstraksi cair-cair Penimbangan bahan sampel ujiBerat kertas = 0,2609 gBerat kertas + zat = 0,7609 gBerat kertas + sisa = 0,2609 gBerat zat= 0,5000 g = 500 mg2. HPLC Penimbangan bahan ParasetamolKafein

Berat kertas (g)

Berat kertas + zat (g)

Berat kertas + sisa (g)

Berat zat (g)

Pembuatan Fase Gerak dan Pelarut Jumlah total 500ml (aquabides : metanol : asam asetat glasial) Aquabides = x 500 = 345 ml Metanol = x 500 = 140 ml Glasial = x 500 = 15 ml

Pembuatan Larutan Intermediet (2ml ke 25 ml)Baku = 2000 ppmC1 V1 = C2 V22000 . 2mL= C2. 25 mLC2 = 160 ppm Larutan Seri 1 C1 V1 = C2 V2160 . 0,5 mL = C2 . 10 mLC2 = 8 ppm (mg/mL)

C1 V1 = C2 V2160 . 1 mL = C2 . 10 mLC2 = 16 ppm (mg/mL) C1 V1 = C2 V2160 . 2 mL = C2. 10 mLC2 = 32 ppm (mg/mL) C1 V1 = C2 V2160 . 3 mL = C2. 10 mLC2 = 48 ppm (mg/mL) C1 V1 = C2 V2160 . 4 mL = C2. 10 mLC2 = 64 ppm (mg/mL)1. KafeinKonsentrasi (ppm)Tf

2. ParacetamolKonsentrasi (ppm)Tf