date: 20/03/16 report on qip course: biology for engineers ...1).pdf · report on qip course:...

G.H.RAISONI COLLEGE OF ENGINNERING, NAGPUR (An autonomous institute affiliated by RTMNU under UGC Act 1956)

Programmes Accreditated by National Board of Accreditation Accreditated by NAAC with “A Grade”

DEPARTMENT OF ELECTRONICS ENGINNERING

1 | P a g e Q I P C o u r s e : B i o l o g y f o r E n g i n e e r : J u n e 6 - 1 1 2 0 1 6

Date: 20/03/16

Report on

QIP Course: Biology for Engineers IIT Bombay –June 6-11 2016

Course Module: Monday 6th June 2016 8:30 am to Saturday 11th June 2016 4:30 pm. Course Coordinator: Dr. Ambarish Kunwar, Bioscience & Bioinformatics Department, IIT Bombay. Course Venue: Vanvihar Guest House Seminar Hall, IIT Bombay.





Day 1 : 6th June 2016

Registration report time: 8:30 to 9am Inauguration: 9am to 9:30 am Lecture 1 Module: Prof. Ambarish Kunwar (10-11:30am) Topic: Forces at microscopic scale in biology

Focus points:

Molecular Motors for transportation. Swimming of Bacteria with microscopic world Protein performs their function by folding 20 Amino acids –proteins making Protein mass measure in Dalton ie. 100KDa 1Da=1Hydrogene New concept for Biology Dashpot Problem Solving on protein Engineering Forces action on Protein molecule. Motion of mechanical element Motion of combination of Mechanical element Motion of combination of mechanical elements Mass and spring with Damping





Lecture 2 Module: Prof. Ambarish Kunwar (11:30am to 1pm) Topic: Thermal and viscous Forces in Biology (Biophysics)

Focus points:

Effect of Medium on Biological System Viscosity and fluid flow Viscous critical force Calculation for Reynolds number Life at low Reynolds number Reynolds Derivation

Lecture 2 Module: Prof. Debashish Chowdhury, IIT Kanpur (2:30pm to 4pm) Topic: Thermal Energy and Brownian Motion

Focus points: Ficks Law + Eqn of Continuity = Diffision Eqn. Fundamental principle : Over damped Motion Dominant forces Einstein Relation Beyond Diffusion: Directed (noisy) Transport Molecular Motor Examples of Molecular motors





Ion motive Force an electro chemical gradient

Fundamental principle : Thermally activated Barrier hopping

Feynmamns Rotary ratchet and pawl device

A Brownian moter does not violate 2nd law of thermodynamics as it operates

far from equilibrium where the 2nd law is not applicable.

Rotary Brownian rachet

www.biologie.uni-

osnabrueck.de/biophys/junge/picture/ATPasevideo/synthase.Mov






Lecture 1 Module: Prof. Kiran Kondabagil (9:30am-11am) Topic: Introduction to cell and cellular process

Evolution and Biology of Extremely Large DNA Viruses Are they alive ? Why are they so big? How do they invade the host? How do they manage to stuff their genome inside the capsid?

Introduction to Cell and Cellular process Characteristics of life





Lecture 2 Module: Prof. Kiran Kondabagil (11:30am-1pm) Topic: Evolution, genetic and chromosomes

Darwin Presented evidence that the today’s organisms are descendants of ancestral species

Darwin proposed a mechanisms for evolutionary process : natural selection Essential for the progress of biology Evolutionary concepts are major part of moderns biology experiments Study of virus evolution (influenza/HIV/H1N1) cancer progression, bacterial drug

resistance etc can save thousands of lives





Lecture 3 Module: Prof. Rahul Purwar (2:30pm-4pm) Topic: Basic Biology of bacteria

Bacteriophages Some virus has envelopes which are membranous convering derived from the

membrane of the host cell. CD4 + T cells –only cell which is infected by HIV in Humans





Lecture 4 Module: Prof. Rahul Purwar (4:30pm-6pm) Topic: Virus Basic Understanding and relevance in medicine

Adoptive T cell therapy: Engineered CAR-T cells

Review of recent clinical trials

Challenges of CAR T cell therapy

Results

T cell subsets: Th17 and Th9 cells in cancer immunotherapy

Target therapy with Tumor specific T cells

Cancer: Melanoma

Autologous tumor infiltrating lymphocytes (TILs); “Live drug”

Advantages

High response rate (>50%),

Long-term remission,

Less toxic & gentler to the patient

Limitation:

Extraction of TILs,

Cell manufacturing

Possible alternate

T cell Engineering (CAR-T cells)






Lecture 5 Module: Prof. Ranjith Padinhateeri (9:30am-11am) Topic: Thermal Bending of Biofillaments

DAN packaging





Energy required to bend DNA





Lecture 6 Module: Prof. Kiran Kondabagil (11:30am-1pm) Topic: From Genes to Protein

Genotype: An organism full hereditary information Phenotype: actual observed properties Genetics of intelligence Genetics and chromosomes Flow of heritable traits

Phenotypic characters that are passed on from parent to offspring E.g. eye and hair color * imagine a future where a test predicts whether you will get cancer. * Today, Uzbekistan is using genetic testing to find future Olympians

Tutorial 1: Prof. Dulal Panda (2:30 pm -4 pm) Topic: Fluorescence spectroscopy, circular Dichroism and FASC in biology

Advantages of Fluorescence Spectroscopy

1. High level of sensitivity and wide dynamic range

2. Single molecule detection

3. Instrument is convenient and modest cost





Spectroscopy is a general term referring to the interactions of various types of electromagnetic radiation with matter. Exactly how the radiation interacts with matter is directly dependent on the energy of the radiation. Spectroscopy: Is an analytical method technique wherein the absorbance of specific wavelength of light by the molecules of sample under test is determined. The more the number of molecules in sample, the greater is the absorbance and vice-verse.

Spectroscopy The higher energy ultraviolet and visible wavelengths affect the energy levels of the outer electrons. Radio waves are used in nuclear magnetic Resonance and affect the spin of nuclei in a magnetic field. Infrared radiation is absorbed by matter resulting in rotation and/or vibration of molecules.

Fluorescence Activated Cell Sorter Machine : FACS






Lecture 7 Module: Prof. Rahul Purwar(9:30am-11am) Topic: Stem cells: Basics Science to Medicine

Antibody “Her2” is used to treat cancer

Stem cells are undifferentiated biological cells that can differentiate into specialized cells and

can divide (through mitosis) to produce more stem cells. They are found in

multicellular organisms. In mammals, there are two broad types of stem cells: embryonic stem

cells, which are isolated from the inner cell mass of blastocysts, and adult stem cells, which are

found in various tissues. In adultorganisms, stem cells and progenitor cells act as a repair system

for the body, replenishing adult tissues. In a developing embryo, stem cells can differentiate into

all the specialized cells—ectoderm, endoderm and mesoderm (see induced pluripotent stem

cells)—but also maintain the normal turnover of regenerative organs, such as blood, skin, or

intestinal tissues.

There are three known accessible sources of autologous adult stem cells in humans:

1. Bone marrow, which requires extraction by harvesting, that is, drilling into bone (typically

the femur or iliac crest).

2. Adipose tissue (lipid cells), which requires extraction by liposuction.

3. Blood, which requires extraction through apheresis, wherein blood is drawn from the

donor (similar to a blood donation), and passed through a machine that extracts the stem

cells and returns other portions of the blood to the donor.





Lecture 8 Module: Prof. Rahul Purwar (11:30am-1pm) Topic: Stem cells: Cell signaling how do cell communicate

Cell use chemical signals for communication Local signaling : the signal acts on nearby cells In a multi cellular organisms : two types of signaling pathways In single cells: three stages of cell signaling Two types of signaling pathways in multicellular organism Signal –receiver (Receptor)

Most of the receptor is plasma membrane proteins for the recognition of ligands: 1. G- protein coupled receptor 2. Receptor tyrosine kinases 3. Ion-channel receptor





Bio Engineering Model

Lecture 9: Module: Prof. Rohit Srivastava (2:30am-4pm) Topic: Stem cells: Introduction to Biomedical Engineering Biomedical Engineering (BME) • Definition2: The use of engineering technology, instrumentation and methods to solve medical problems, such as improving our understanding of physiology and the manufacture of artificial limbs and organs. Diversity in the terminology • (bio)medical engineering, • bioengineering, biotechnology • clinical (medical) engineering • medical technology. • health care technology





Application of BME

Artificial heart (20.4 month recoed) Artificial Kidney (not design yet) Dialysis machine Mems Design Artificial insule pain Artificial urinary bladder Artificial Hips Joint replacement (titanium alloy) Bionic Arm *Mr. Robert Langer –1000 Patent (Chemical Engienner)

In View of Electronics Engineering

In view of Mechanical Engineering





Lecture 10: Module: Prof. P.V.Balaji (4:30am-6pm) Topic: Stem cells: Proteon Structure – Free Energy and Entropy and their role

Protein performs their function by folding into different shapes 20-Aminoa acids

Entropy S =K g ln W Where, W= no of microscopic arrangement

Two macro & micro state of a folded state Probability of a folding a protein a micro or Macro state





Protein Folding






Lecture 11 Module: Prof. Soumyo Mukherji (9:30am-11am) Topic: Electrophysiology

Heart beats rate -72 per minute Per day= 103680 almost 1 lack Per year = 37843200 about 3.75 cores Over a life time = 245 cores (in about 65 year)





Lecture 12 Module: Prof. Prakriti Tayalia (11:30am-1pm) Topic: Tissue Engineering





Lecture 13 Module: Prof. Neeta Kanekar (2:30pm-4pm) Topic: Movement Science and Rehabilitation Engineering





Tutorial Lecture 14 Module: Prof. Dulal Panda (4:30pm-6pm) Topic: Fluorescence Specroscopy, Circular Dichroism and FACS in Biology – Part- II

Dependence of Fluorescence Polarization on Molecular Mobility

(1/P -1/3) = (1/P0 -1/3) (1 + τ/Ø)






Lecture 15 Module: Prof. (Dr.) V.P.Soni (9:30am-11am) Topic: Bioengineering concepts in Maxillo-facial Surgery

Engineering principles in surgical reconstruction Poly lactic polyalglamotate is the biodegradable

material used CAD-CAM Technology used to design Computer Assisted Surgery Gama Radiation used to Sterilize polymer graft for

body





Lecture 16 Module: Prof. Ranjith Pandinhateeri (11:30am-1pm) Topic: Tutorial –II (Problem Solving)

Day 6 : 11th June 2016 : Valedictory Function and Certificate Distribution





Biology + Engineering = Good idea*

date: 20/03/16 report on qip course: biology for engineers ...1).pdf · report on qip course:...

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