date of birth : tegal (central java), 23 september 1933qualifications: 1938 – 1947: elementary...

Date of Birth : Tegal (Central Java), 23 September 1933

QUALIFICATIONS:

1938 – 1947 : Elementary School1948 – 1951 : High School1951 – 1958 : Medical Faculty at University of Indonesia, Graduated in 19581958 – 1961 : University of California, San Francisco, Ph.D. degree in Pharmacology. Thesis: The Inhibition of Alcohol Dehydrogenase by Chlorpromazine and other Phenothazine Derivates

MEMBERSHIP:

1970 - 1990 : Member/Founder Yayasan Pengembangan Sumber Daya Manusia1996 - 2006 : Member Komnas Perempuan1985 - Present : Member/Founder Yayasan Pengembangan Kreativitas1990 - Present : Member Yayasan Keanekaragaman Hayati Indonesia1990 - Present : Ketua Yayasan Pendidikan Kalbe2005 - Present : Member Komnas Lansia2005 - Present : Ernst & Young Lifetime Entrepreneurial Award2008 - Present : Vice Chairman of Advisory Board, Indonesian Stem Cell Association / Asosiasi Sel Punca Indonesia (ASPI)2010 : Honorary Reviewer Ristek Kalbe Science Award (RKSA)

Curriculum Vitae

Dr. Boenjamin Setiawan, Ph.D.

Boenjamin Setiawan,dr.,Ph.D. 2

Slowing Down The Aging Process with Stem Cells

Boenjamin Setiawan, dr., Ph.D.Chairman of Scientific Advisory Board

Stem Cell and Cancer Institute

Presentasi 16 Sept.2011, FIMA, YARSI

When Does Ageing Begin?

Ageing begins the day we are born There is no single measure of how

“old” a person is Ageing is highly individualized Ageing proceeds at different rates in

different people, and within different systems of the body

Ageing is GENETIC and the secret is stored in the DNA

Why Do People Age?

Many theories to include: Hereditary Factors, stored in DNA Loss of cellular mass and ability of cells to

divide and replicate, STEM CELLS Accumulation of waste materials that clog

cells and cause them to die, TELOMERASE

Oxidative damage as a result of normal METABOLISM caused by FREE RADICALS, ROS, use ANTIOXIDANTS

Changes in structure of connective tissueNo single theory adequately describes the

aging process

Normal Changes of Aging

Physical changes related to “Normal” aging ARE NOT disease Changes occur in most body systems to include:

1. Sensory System: Hearing, Vision, Balance, Taste buds, etc.

2. Cardiovascular system3. Central Nervous System4. Skin, Muscles and Bones5. Digestion system6. Respiratory system7. Immune system8. Endocrine system9. All Organ System starts to Degenerate


The Theories and Major Causes of Age-related Death are:


1. Oxidative Damage – ANTI-OXIDANTS2. Cell DNA Damage3. Mitochondrial Damage4. Lipofuscin Accumulation5. Chronic Inflammation - MESENCHYMAL STEM CELLS6. Immune System Compromise - MSC7. Neurological Degeneration8. Declines in Hormone Levels–Growth Hormone, Testosteron9. Susceptibility to Cancers10. Susceptibility to Cardiovascular Disease11. Telomere Shortening and Damage12. Programmed Epigenomic Changes13. Stem Cell Supply Chain Breakdown–STEM CELL SUPPLY


The Life Sciences Revolution in the News

Stem Cell Therapy Regenerative Medicine Human Cloning Genomics Induced Pluripotent SC The Human Genome Project Genetically Modified Crops Biological Agents in

Bioterrorism


Special edition to celebrate the 50th anniversary of the discovery of

DNA: February 17, 2003

Boenjamin Setiawan,dr.,Ph.D. 8Presentasi 16 Sept.2011, FIMA, YARSI

Global Aging

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500

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1960 1990 2020 2050

WORLD POPULATION AGED 60 YRS AND OVER IN MILLIONS


“Force of mortality” (hazard rate) increases with age



Aging is a Very General Phenomenon!



According to Reliability Theory:

Aging is NOT just growing oldInstead

Aging is a degradation to failure: becoming sick, frail and dead

'Healthy aging' is an oxymoron like a healthy dying or a healthy disease

More accurate terms instead of 'healthy aging' would be a delayed aging, postponed aging, slow aging, or negligible aging (senescence)Presentasi 16 Sept.2011,

FIMA, YARSI


World’s oldest person celebrates 129th birthday, died March 2007

According to national records, Hernandez was born on May 3, 1878, in one of the country's central provinces, where she gave birth to 13 children. She now has 60 grandchildren, 80 great-grandchildren and 25 great-great grandchildren.


The Human Body Consist of 12 Organ Systems Consist of 220 types of Tissues Consist of 50-100 trillion cells Consist of 100 billion neurons Consist of 46 chromosomes (22 pairs and

x and y chromosomes) Consist of 30.000 genes Consist of 3,2 billion base pairs



50-100 Trillion Cells form the Building Block of the Human Body


Multiple embryos are produced in vitro for clinical purposes.Donated surplus embryos are used as a source of ES cells.These surplus embryos would otherwise be discarded or remain unusedPresentasi 16 Sept.2011, FIMA, YARSI

How are ES Cells Generated?


Fertilized Embryos Are Allowed to Divide Several Times in Culture



Harvesting ES Cells


The blastocyst is the stage of development (~1,000 cells) at which the embryos inner cell mass (ICM) forms. It is the ICM that harbors ES cells. Stem cells exist only fleetingly at this stage of development


Where Are Early Stem Cells Found?


5 days ofdevelopment

Who did it first?

• In 1998, Prof. James Thomson,U. Wisconsin research team isolated stem cells from IVF-blastocysts


Stem Cells are

taken from the Inner Cell Mass at

the blastocyst stage and cultured in a Petri

diskPresentasi 16 Sept.2011, FIMA, YARSI


Embryonic Stem cells: - spare embryos, from IVF

- special purpose embryos

- cloned embryos

Adult stem cells: - adult tissue, - Bone marrow, Peripheral blood,

Adipose tissue, Lung, Liver, Pericytes

Other sources: - aborted fetuses

- umbilical cords

Sources of stem cells


Stem Cell and Cancer Institute Capabilities, SCI (Indonesia)

1. Culturing and multiplications of hUCB stem cells for BURNS and CHRONIC WOUNDS

2. Culturing and multiplications of Chondrocytes for Osteoarthritis

3. Culturing and multiplication of Autologous MNC/EPC from Peripheral blood for AMI and Heart failure

4. Culturing hUC. MSC for Stroke and Cerebral Palsy

5 SCI Developed VascGrow a Cocktail to Grow and Differentiate Human EPC



Reagents and consumables Controlled storage Controlled temperature for refrigeratorsControlled SOPs for proceduresCertificate of analysis

Cell Banking, Processing and Analysis Facility


Stem Cells Isolation from Lipoaspirates for further Research Application

- Routinely prepares Mesenchymal Stem Cell isolation from lipoaspirate

- Collaborates with several Plastic surgeons- On going research to study several distinct molecules

HLA-Class II CD45 CD34 CD105

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(B) (C)

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200

160

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40

(A) (D)

Cultured cells Isotype control

101 102 103 104 100 101 102 103 104 100


Clinical GradeStandardized proceduresProvide cells for stem cells clinical trialsAllogeneic & autologous

Cell Banking, Processing and Analysis Facility

Cryopreservation facility (vapour phase)Controlled Transported samples (temperature logger)Complete report of product quality and evaluation GMP Facility


SCI projects Stem Cell Research Division1. Cell Analysis, Processing and Banking Facility

2. Immunogenicity Characterization of Mononucleated Cells Originated from UCB

3. Endothelial Progenitors Cell Expansion

- scientific explanation for allogeneic UCB MNC therapy

- Determination of EPCs frequency as biological marker for vascular function & cardiovascular disease risk

- MI, Rejuvenation, & anti-aging4. Stem Cells Isolation from Lipoaspirates for further Research Application


Applications: Heart Failure in Harapan Kita Hospital

Blood Collection

1

3

Medium

2 EPC culture

VascGrow

Ready for EPC implantation

Cells are collected and washed


COMMERCIALIZATION Windpipe Transplant


Rat heart decellularization (top three images), and during recellularization (bottom two images). Experiment and

photos: November 2006. (Credit: Thomas Matthiesen)


Beating Rat Heart Created In Laboratory: Method May Revolutionize How Organ Tissues Are Developed

ScienceDaily (Jan. 14, 2008) — University of Minnesota researchers have created a beating RAT heart in the laboratory


Stages of Regenerative Medicine, 5R

1. Repair, Molecular Changes in the Microenvironment through Paracrine Secretions of STEM CELLS

2. Replacement of dead cells with somatic stem cells in affected tissues/organs

3. Regeneration of all the functions of the affected tissues/organs

4. Rehabilitation of all the tissues/organs in a multi-organ failure

5. Rejuvenation the dream of everybody to be able to rejuvenate all the molecules, cells, tissues/organs of the body through changes/reprogramming of the DNA molecule with a protein cocktail (elixir of life)Presentasi 16 Sept.2011,

FIMA, YARSI


Target Diseases for Stem Cell Therapy

Blood Diseases Bone marrow transplants (BMT) are a well known clinical

application of hematopoietic stem cell (HSC) therapy HSCs can regenerate all of the different cell types in blood BMT is used for the treatment of blood cancers like

leukemia and lymphoma, as well as breast cancer and any other disease requiring immune system regeneration



The cardiovascular circulatory system’s main role is to transport nutrients and oxygen to the 50-100 trillion cells that makes up our body and remove the waste material from the body.

The most prevalent disease that affects the CVS is:

1. Atherosclerosis2. Hypertension3. Acute myocardial

infarction4. Stroke


Major Role of CNS: The main role of the nervous system is to relay electrical signals through the body. The nervous system directs behaviour and movement and, along with the endocrine system, controls physiological processes such as digestion, circulation, etc.

The most prevalent diseases that affect the CNS is Stroke, Parkinson’s disease, Alzheimer’s disease, ALS and Autism


The brain microvascular endothelium constitutes a major exchange area between the blood and the brain: it forms a physiological barrier, known as the blood-brain barrier (BBB), which plays a central role in maintaining cerebral homeostasis.


Spinal Cord Injuries


Hwang Mi-Soon: South Korea

Paralyzed 19 years

Multipotent adult stem cells injected into her spinal cord

Currently: debilitating pain

Published in 2005 (Cytotherapy)

46Presentasi 16 Sept.2011, FIMA, YARSI Boenjamin Setiawan,dr.,Ph.D.

Ischemic Stroke


Target Diseases for Stem Cell Therapy

Type 1 Diabetes

Patients lack pancreatic beta cells and cannot produce insulin

Insulin-producing structures similar to pancreatic islets have been generated from mouse ES cells in culture

It may also be possible to isolate pancreatic stem cells from adult tissue

Early stage of development



Osteoarthritis and Autoimmune Diseases

Mesenchymal stem cells are used to be inserted into the synovial cavity where they transdifferentiate into chondroblasts and form new cartilage



Shinya Yamanaka & James Thomson

Shinya Yamanaka, 45, working in Japan's Kyoto University with mouse cells, made the iPS breakthrough. He screened 24 candidate proteins before finding four that were able to reprogram adult cells, reverting them to their embryonic state. He and others then showed that these factors are also effective in human cells.

James Thomson,49, a Development Biologist of the University of Wisconsin was the first to identify a slightly different group of factors that do the same



Slowing Down the Aging Process1. CRAN = Caloric Restriction Adequate Nutrition,

Healthy DIET, Vegetables/ FRUITS2. Regular Exercise, Walking and Weight Lifting3. Have ACTIVE LIFESTYLE, WORK, NEVER RETIRE

!!4. Use BRAIN ACTIVELY, use INTERNET5. Sharpen your SAW, have a regular BREAK6. Take VITAMINS, ANTI-OXIDANTS, MINERALS

and Hormones7. HAVE A POSITIVE MIND, BE OPTIMISTIC !!!8. BE HAPPY ALL THE TIME !!!9. Use of Mesenchymal Stem Cells to slow

down the Ageing Process



Conclusions1. Adult stem cells can be obtained from

BM, hUCord, Dental pulp, Adipocytes and Peripheral blood, It will not create any ethical problems

2. Adult stem cells from fetuses and hUC are the most PLASTIC

3. Embryonic stem cells obtained from the INNER CELL MASS for THERAPEUTIC purposes are allowed in 38 countries (UK, Sweden, Belgium, Netherland, China, India, Korea, Taiwan, Singapore, Malaysia etc.)

4. Embryonic stem cells for reproductive cloning is not allowed in ALL COUNTRIESPresentasi 16 Sept.2011,

FIMA, YARSI


5. Mesenchymal Stem Cells from BM, Fetuses, hUCord, can be used as Progenitor Neuronal Cells for transplantation in Neurodegenerative Diseases.

6. EPCs as well as MSC from BM, hUCord, Adipocytes and peripheral blood can be used for AMI, Heart Failure, Auto-immune diseases, and can clear Sclerotic Plaques in Arteries, etc.

7. Stem Cell Research should become a PRIORITY to be carried out in Indonesia and the rest of the WORLD to SLOW DOWN THE AGEING PROCESS

8. Academic Institutions, Business and Government (ABCG) must Cooperate Closely to Stimulate RND for the Benefit of Society


5. Mesenchymal Stem Cells from BM, Fetuses, hUCB, can be used as Progenitor Neuronal Cells for transplantation in Neurodegenerative Diseases.

6. EPCs as well as MSC from BM, hUCB, Adipocytes and peripheral blood can be used for Acute Myocardial Infarction

7. Stem Cell Research should become a PRIORITY to be carried out in Indonesia

8. Academic Institutions, Business and Government (ABCG) must Cooperate Closely to Stimulate RND for the Benefit of Society


Thank YouLive with the 5 E’s:Energy, Enable, Enthusiasm, Empower, and Enrich.

date of birth : tegal (central java), 23 september 1933qualifications: 1938 – 1947: elementary...

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