dengue virus antibodies - sapphire bioscience€¦ · dengue virus antibodies the dengue virus ......

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~~

() GeneTex ~

Dengue Virus Antibodies

The Dengue virus (DEN) is of the genus Flavivirus and possesses a

posit ive-strand RNA genome of approximately 10.6 kb that conta ins a ~

single open-reading frame encoding for a polyprote in, which can be I ' further cleaved by cellular and viral proteases into three structural

proteins (capsid prote in C, membrane protein M, envelope protein

E) , and seven non-structural proteins (NS1 , NS2a , NS2b , NS3 ,

NS4a , NS4b, NS5). The re are four serotypes (DEN1 -4) , though

there is certa inly genotypic variability in individual viral genomes

within each serotype . This variability is thought to have minimal

clinical significance, as human infection from any of the four serotypes

results in long -term immunity to that serotype . However, cross-i mmun ity between serotypes is of much shorter

duration , meaning that many people are victims of sequential infection by different serotypes . This is clinically

very important , as sequential infection appears to be a risk factor for more serious disease manifestations.

Dengue Virus Protein Schematic Diagram

II. ___ .11 Capsid Prm Envelop NS1 NS28 NS3 NS4A NS48

(GTX103343) (GTX 128093) (GTX629116) (GTX630556) (GTX 124246) (GTX629477) (GTX 124249) (GTX124250)

kDa

NS5 (GTX629447)

kDa 72-55-43-

34-26 -

17-

kDa 72- A B 55-43-34-

26- -17-

kDa A B

70 -55-40-

35-25-

WB analysis of virus-infected BHK21 cells.

kDa i30- A B

100 -

70-

55 -

40 -

35-

-kDa A B 95-72 -55 -43 -

34-

26 -

WB analysis with GeneTex dengue virus antibodies on virus-infected BHK21 cells.

NS1 (Dengue virus) antibody [GT1061] (GTX630557) ICC/IF with NSl (Dengue virus) antibody on BHK-21 cells infected with Dengue 2 virus.

kDa 170- A B

130-

95-

72-

55--

kDa 130- ABC 72 -55-43-34-26-

- +-17-

10-

Mock

i30- A B

95-72 -

55-

43 -

34-

26-

130 -100-70-

55-

40-

35-

25_

A B

Dengue 2 virus infected

NS3 (Dengue virus) antibody [GT2811] (GTX629477) ICC/IF with NS3 (Dengue virus) antibody on BHK-21 cells infected with Dengue 2 virus.

www.genetex.com

•

• •

• • • • • •

I

I

I , , ,

I

Mock

20~m

20~m

NS4B (Dengue virus) antibody (GTX1 03349) ICC/IF with NS4B (Dengue virus) antibody on BHK-21 cells infected with Dengue 2 virus.

N

'" o <Xl N ,....,

~ \!l

I

I I

I

I

Mock

20~m

20~m

PrM (Dengue virus) antibody (GTX128092) ICC/IF with PrM (Dengue virus) antibody on BHK-21 cells infected with Dengue 2 virus.

C!malH!l. 1;Ojf'!DIiI1

., GTX29202 Dengue virus 1, 2, 3 &4 antibody [D1·11(3)]

GTX41428 Dengue Virus Type 2 antibody [NYRDeng2]

GTX43295 Dengue Types 1,2,3,4 antibody [BDI419]

GTX41437 Dengue Virus antibody [5161]

GTX41430 Dengue Virus Type 2 antibody [3H5.1]

GTX43296 Dengue Type 2, envelope antibody [B932M]

~ GTX124247 Capsid Protein (Dengue virus) antibody

GTX103343 Capsid Protein (Dengue virus) antibody

GTX128092 PrM (Dengue virus) antibody

~ GTX128093 PrM (Dengue virus) antibody

GTX127277 Envelope protein (Dengue virus) antibody

GTX629116 Envelope protein (Dengue virus) antibody [GT214]

GTX629117 Envelope protein (Dengue virus) antibody [GT643]

GTX630557 NS1 (Dengue virus) antibody [GT1061]


~ GTX103346 NS1 (Dengue virus) antibody

GTX124280 NS1 (Dengue virus) antibody

~ GTX124246 NS2B (Dengue virus) antibody



~ GTX124249 NS4A (Dengue virus) antibody

GTX103349 NS4B (Dengue virus) antibody

~ GTX124250 NS4B (Dengue virus) antibody


GTX124253 NS5 (Dengue virus) antibody

GTX629446 NS5 (Dengue Virus 2) antibody [GT353]

GTX629447 NS5 (Dengue virus 2) antibody [GT361]

Mock Dengue 2 virus infected

Envelope protein (Dengue virus) antibody [GT643] (GTX629117) ICC/IF with Envelope protein (Dengue virus) antibody on BHK-21 cells infected with Dengue 2 virus.

Mock Dengue 3 virus infected

Capsid Protein (Dengue virus) antibody (GTX124247) ICC/IF with Capsid Protein (Dengue virus) antibody on BHK-21 cells infected with Dengue 3 virus.

.. ~ • ' .. • DEN Ms mAb Dot, ELISA, ICCIIF, WB

DEN2 Ms mAb ELISA, WB

DEN Ms mAb Dot, ELISA, ICCIIF

DEN Ms mAb ELISA

DEN2 Ms mAb ICCIIF

DEN2 Ms mAb ICC/IF

Capsid (DEN) Rb pAb ICCIIF, WB

Capsid (DEN) Rb pAb ICCIIF, WB

PrM (DEN) Rb pAb ICC/IF , WB

PrM (DEN) Rb pAb ICCIIF, WB

Envelope (DEN) Rb pAb ICCIIF, WB

Envelope (DEN) Ms mAb ICC/IF, WB

Envelope (DEN) Ms mAb ICCIIF, WB

NS1 (DEN) Ms mAb ICCIIF, WB


NS1 (DEN2) Rb pAb ELISA, ICCIIF, WB

NS1 (DEN) Rb pAb ICCIIF, WB

NS2B (DEN) Rb pAb ICCIIF, WB

NS3 (DEN) Rb pAb ICC/IF , IHC·P, WB


NS4A (DEN) Rb pAb ICC/IF , WB

NS4B (DEN) Rb pAb ICC/IF, WB

NS4B (DEN) Rb pAb ICCIIF, WB

NS5 (DEN) Rb pAb ICC/IF , WB

NS5 (DEN) Rb pAb ICC/IF , WB



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