DEPARTMENT OF CHEMISTRY AND ENVIRONMENTAL SCIENCE

SEMINAR SERIES

FALL 2018

DATE: THURSDAY, DECEMBER 6, 2018

LOCATION: TIERNAN HALL – 373

TIME: 2:00 PM – 3:30 PM

GUEST SPEAKER Naijun Wu

Analytical Development

Celgene Corporation

Summit, NJ

TOPIC High Speed Chromatography to Expedite Pharmaceutical Development

ABSTRACT Fast chromatography has played a critical role in expediting pharmaceutical development during

the last decade. In this presentation, various approaches to fast separation are demonstrated, their

mechanisms are discussed, and high throughput screen systems are described.

In coupling with ultra-high-pressure liquid chromatography (UHPLC) instrumentation, short

columns packed with small particles and superficially porous packings have been used for fast

liquid chromatographic separations. These column packing materials provide much lower mass

transfer resistance and thus high-speed separation. Small particle packed columns also provide

advantages for high speed separation in supercritical fluid chromatography (SFC). Additionally,

SFC provides better selectivity and thus faster separation for chiral compounds compared to

capillary gas chromatography (GC). Small internal diameter columns and / or hydrogen carrier

gas can be used to increase separation speed in GC. Separation times were reduced from 30 – 60

min to seconds.

It was demonstrated that high throughput screen systems with DoE software could speed up

method development and optimization.

BIOGRAPHY Dr. Naijun Wu received his Ph.D. degree in Analytical Chemistry in 2000 from Brigham Young

University, Provo, Utah, under the direction of Professor Milton Lee, where he conducted

pioneering research on Ultra-High Pressure Liquid Chromatography (UHPLC) or Ultra-high

Performance Liquid Chromatography (UPLC) as well as fast gas chromatography (GC) and fast

Supercritical Fluid Chromatography (SFC). He also holds a BS degree and an MSc degree in

Chemistry from Nanjing University of Technology.

Dr. Wu has authored and co-authored more than 50 papers and given more than 40 presentations

at National and International Scientific Conferences. He has also authored three book chapters

and multiple invited reviews. He is current editorial board member of Journal of Liquid

Chromatography and Related Technologies. His research has focused on high-speed LC

separation using UHPLC /UPLC and novel column technologies for pharmaceuticals. His other

research interests include fast LC screening, fast GC, fast chiral SFC, and thermodynamics in

HPLC.

Dr. Wu is currently Director of Analytical Development, Celgene Corporation, directing 20+

Ph.D. and M.Sc. scientists on analytical research and development. He has more than eighteen

years of leadership and scientific experience in analytical R&D functions with increasing

responsibilities in supporting drug substance and formulation development, process scale-up,

technology transfer, regulatory registration and in-line supply of API and solid and parenteral

products from pre-clinical to registration phases. He is managing all the analytical aspects

including method development, validation release testing, stability, supporting API and

formulation development, and filings. Priorto Celgene, he worked with Merck for 12 years in

Analytical R&D Department. Between1990 and 1995, he worked as an assistant professor and

assistant chair at Applied Chemistry Department of Nanjing University of Technology.

Naijun Wu’s Publications Book Chapters

1. Naijun Wu, Christopher J. Welch, Theresa K. Natishan, Hong Gao, Tilak Chandrasekaran, and

Li Zhang, Chapter 3: “Practical Aspects of Ultrahigh Performance Liquid Chromatography,” in

Ultra-High Performance Liquid Chromatography and Its Applications, Ed. By Alan Xu, p.40-94,

2013, John Wiley & Sons, Inc., Hoboken, New Jersey.

2. Wu, N.; Li, Z. "Applications of Monolithic Chromatography in Support of Pharmaceutical

Development of Drug Substances and Formulations," in "Monolithic Chromatography and Its

Modern Applications", Ed. by Perry Wang, ILM Publications, Chapter 15, p.427-449, 2010.

3. Wu, N. “Increasing the Speed of Enantiomeric Separation Using Supercritical Fluid

Chromatography,” in Advances in Chromatography, Schmidt Periodicals GmbH, Germany,

2007, Vol. 46, Chapter 4.

Peer-reviewed Papers 1. T. Nowak, G. C. Graffius, Y. Liu, N. Wu, X. Bu, X. Gong, C. J. Welch, E. L. Regalado, “GC-

FID method for high-throughput analysis of residual solvents in pharmaceutical drugs and

intermediates.” Green Chem., 2016, 18, 3732-373

2. M Biba, EL Regalado, N Wu, CJ Welch, “Effect of particle size on the speed and resolution of

chiral separations using supercritical fluid chromatography,” J. Chromatogr. A, 2014, 1363, 250-

256.

3. Wu, N.*; Bradley, A. C. “Effect of column dimension on observed column efficiency in ultra-

high pressure liquid chromatography,” J. Chromatogr. A 2012, 1261:113-120.

4. Wu N, Bradley AC, Welch CJ, Zhang L., “Effect of extra-column volume on practical

chromatographic parameters of sub-2-µm particle-packed columns in ultra-high pressure liquid

chromatography,” J. Separation Science. 2012, 35, 2018-2015.

5. Welch, C.J.*; Wu, N.*; Biba, M.; Hartman, R.; Brkovic, T.; Gong, X.; Helmy, R.; Schafer,

W.; James Cuff, J.; Pirzada, Z; Zhou, Z. "Greening Analytical Chromatography," Trends in

Analytical Chemistry, 77(29), 667-680, 2010.

6. Ahmed Abrahim, Mohammad Al-Sayah, Peter Skrdla, Yuri Bereznitski, Yadan Chen, Naijun

Wu*, "Practical Comparison of 2.7 .m Fused-Core Silica Particles and Porous Sub-2 .m Particles

for Fast Separations in Pharmaceutical Process Development", J. Pharm. Biomed. Anal. 2010,

51, 131-137.

7. Wu, N*; Clausen, A.; Wright, L.; Vogel, K.; Bernardoni, F. "Fast UHPLC Using Sub-2 µm

Particles and Elevated Pressures for Pharmaceutical Process Development”. American

Pharmaceutical Review 2008, 11, 24-33.

8. Al-Sayah, A.A.; Rizos, P.; Antonucci, V.; Wu, N. "High throughput screening of active

pharmaceutical ingredients by UPLC," J. Sep. Sci. 2008, 31 (12), 2167-2173.

9. Wu, N; Clausen, A "Recent Development of Ultra-high Pressure Liquid Chromatography,"

CACS Communications 2008, 2(1), 15-21.

10. C.J. Welch, C.J.; Hyun, M.H.; Kubota, T.; Schafer, W; Bernardoni, F.; Choi, H.J.; Wu, N.;

Gong, X.; Lipshutz, B. Microscale HPLC enables a new paradigm for commercialization of

complex chiral stationary phases, Chirality, 2008, 20 (7), 815-819

11. Wu, N; Clausen, A. Invited Review: “Fundamental and practical aspects of ultrahigh

pressure liquid chromatography for fast separations”. J. Sep. Sci. 2007, 30, 1167-1182.

12. Wu, N.; Liu, Y.; Lee, M.L. “Sub-2mum porous and nonporous particles for fast separation in

reversed-phase high performance liquid chromatography.” J. Chromatogr. A 2006, 1131, 142-

150

13. Wu, N.; Thompson, R. “Fast and Efficient Separations Using Reversed Phase Liquid

Chromatography,” J. Liquid Chromatography, 2006, 29, 949-988.

14. Duran, D; Wu, N.; Mao, B.; Xu, J. "Application of Fast Reversed Phase Liquid

Chromatography for Analysis of Pharmaceutical Related Boronic Acid and Boronic Pinacol

Ester Functionalized Compounds," J. Liquid Chromatogr. Rel. Tech., 2006, 29, 661-672

15. Tang, Q.; Wu, N.; Yue, B.; Lee, M.L. “Small Diameter (3µm), Large Pore (1500Å)

Octadecylsilica for Capillary Electrochromatography,” Chromatographia, 2005, 61, 345-350.

16. Liu, Y.; Antonucci,V.; Shen, Y.; Vailaya, A.; Wu, N.* " Practical Applications of Monolithic

Columns to Pharmaceutical Process Development" J. Liquid Chromatography, 2005, 28, 361-

376.

17. Wu, N. ; Dempsey, J.; Yehl, P.M.; Dovletoglou, A.; Ellison, D.K.; Wyvratt, J.M. “Practical

Aspects of Fast HPLC Separations for Pharmaceutical Process Development Using Monolithic

Columns ” Analytica Chimica Acta, 2004, 523, 149–156.

18. Wu, N.; Yehl, P.M.; Gauthier; D., Dovletoglou, A. "Retention and Thermodynamic Studies

of Piperazine

Diastereomers in Reversed-Phase Liquid Chromatography," Chromatographia, 2004, 59, 189.

19. Wu, N.; Gauthier; D., A. Dovletoglou; Yehl, P.M.; "Mechanistic Studies of the Separation of

an HIV Protease Inhibitor from its Piperazine Diastereomer by HPLC," J. Liquid

Chromatography, 2003, 26, 3343-3355.

20. Gauthier, D.R.; Ikemoto N., Fleitz, F. J., Szumigala, R.H.; Petrillo, D.; Liu, J.; Armstrong,

J.D.; Yehl, P.M.; Wu, N. and Volante, Total synthesis of a second generation HIV protease

inhibitor, Tetrahedron Asymmetry, 2003, 14, 3557-3567.

21. Xian, Y.; Wu, N.; Lippert, A.J.; Lee, M.L. “Separation of Chiral Pharmaceuticals Using

Ultrahigh Pressure Liquid Chromatography,” Chromatographia 2002, 55, 399-403.

22. Wu, N.; Tang, Q.; Lippert, A.J.; Lee, M.L. “Packed Capillary Column Solvating Gas

Chromatography Using Neat Water Mobile Phase and Flame Ionization Detection,” J. Microcol.

Sep. 2001, 13, 41-47.

23. Medina, J.C.; Wu, N.; Lee, M.L. “Comparison of Empirical Peak Capacities for High-

Efficiency Capillary Chromatographic Techniques,” Analytical Chemistry 2001, 73, 1301-1306.

24. Wu, N.; Lippert, A.J.; Lee, M.L. “Practical Aspects of Ultrahigh Pressure Liquid

Chromatography” J. Chromatogr. 2001, 911, 1-12.

25. Wu, N.; Richard, Y.; Lee, M.L. “Fast Supercritical Fluid Chromatography of Polymers Using

Packed Capillary Columns,” Chromatographia 2001, 53, 197-200.

26. Wu, N.; Medina, J.C.; Lee, M.L. “Fast GC: Packed Capillary Solvating GC versus Open

Tubular GC,” J. Chromatogr. A 2000, 892, 3-13.

27. Wu, N.; Collins, D.C.; Lippert, A.J.; Xiang, Y.; Lee, M.L. “Ultrahigh Pressure Liquid

Chromatography/Time-of-Flight Mass Spectroscopy for Fast Separations” J. Microcol. Sep.

2000, 12, 462-469.

28. Tang, Q.; Wu, N.; Lee, M.L. “Continuous-bed Columns Containing So-gel Bonded

Octadecylsilica for Capillary Liquid Chromatography,” J. Microcol. Sep. 2000, 12, 6.

29. Collins, D.C.; Tang, Q.; Wu, N.; Lee, M.L. “Fast LC-TOFMS Using Sol-gel Bonded

Continuous-Bed Capillary Columns.” J. Microcol. Sep. 2000, 12, 442-449.

30. Shen, Y.; Wu, N.; Tang, Q.; Chen, Z.; Bradshaw, J.S.; Lee, M.L. “Effect of Pore Size on

Speed of Chiral Separations Using Packed Capillary SFC,” J. Microcol. Sep. 2000, 12, 475-481.

31. Wu, N.; Chen, Z.; Medina, J.C.; Bradshaw, J.S. Lee, M.L. “Fast Chiral Separations Using

Near-Critical Carbon Dioxide,” J. Microcol. Sep. 2000, 12, 454-461.

32. Wu, N.; Tang, Q.; Shen, Y.; Lee, M.L. “Porous and Nonporous Particles in Solvating Gas

Chromatography,” Analytical Chemistry 1999, 71, 5084-5092.

33. Wu, N.; Tang, Q.; Shen, Y.; Lee, M.L. “Polybutadiene-Coated Zirconia Packing Materials in

Solvating Gas Chromatography Using Carbon Dioxide as Mobile Phase,” Chromatographia

1999, 49,

431-436.

34. Wu, N.; Shen, Y.; Lee, M.L. “Fast Solvating Gas Chromatography of Environmentally

Important Compounds Using Polymer-encapsulated Silica Particles,” J. High Resol. Chromatogr.

1999, 22, 541-546.

35. Tang, Q.; Shen, Y.; Wu, N.; Lee, M.L. “Situ Crosslinked Polybutadiene-Coated Zirconia as a

Monolithic Column for Fast SGC,” J. Microcol. Sep. 1999, 11, 415.

36. Tang, Q.; Wu, N.; Lee, M.L. “Continuous-bed Columns Containing So-gel Bonded Large-

Pore Octadecylsilica for Capillary Electrochromatography,” J. Microcol. Sep. 1999, 11, 550.

37. Lippert, A.J.; Xin, B.; Wu, N.; Lee, M.L. “Fast Ultrahigh-Pressure Liquid Chromatography:

On-column UV and Time-of-Flight Mass Spectrometric Detection,” J. Microcol. Sep. 1999, 11,

631.

38. Hu, K.; Bradshaw, J.S.; Dalley, N.K.; Krakowiak, K.E.; Wu, N.; Lee, M.L. “Synthesis of a

Chiral Macrocyclic Dibenzodicyclohexanotetraamide-Containing Stationary Phase For Liquid

Chromatography,” J. Heterocyclic Chem. 1999, 36, 381.

39. Wu, N.; Shen, Y.; Lopez-Avila, V.; Lee, M.L. “Separations of Perfluorinated Polyethers

Using Packed Capillary Column Supercritical Fluid Chromatography,” J. Microcol. Sep. 1999,

11, 287-297.

40. Wu, N.; Shen, Y.; Lee, M.L. “Comparison of Packed Capillary Solvating Gas

Chromatography with Supercritical Fluid Chromatography Using Carbon Dioxide as Mobile

Phase,” Chromatographia 1998, 47, 673.

41. Wu, N.; Wang, Z.; Xiu, M. “Determination of Trace Phosphorus in Water Using a Rhodamin

B-Phosphomolybdenotungstate-PVA System,” Journal of Nanjing Institute of Chemical

Technology (Chinese), 1993, 15, 24.

42. Wu, N.; Wang, S.; Wang, Z.; Xu, H.; Fang, J. “Application of Keggin Heteropoly Acids in

Passivation of Zinc-plating Coating,” Yingyong Huaxue (Chinese J. Appl. Chem.) 1993, 10, 5.

43. Fang, J.; Wu, N.; Wang, Z. “Activation Effect of Halides on Chromium Electrodeposition

from Chromic Acid Baths,” J. Appl. Electrochem. 1993, 23, 495.

44. Wu, N.; Wang, Z.; Fang, J. “Studies of Heteropolyanions by XPS (III). Studies on Difference

in Thermostability between Molybdic Acids and Vanadic Heteropolyacids,” Journal of Nanjing

Institute of Chemical Technology (Chinese), 1992, 14, 13.

45. Wang, Z.; Wu, N.; Wang, S. “Synthesis and Characterization of Heteropoly-tungstosilicated

Complexes with Lanthanide Elements,” Chem. Res. in Chinese Univ. 1992, 8, 343.

46. Wang, Z.; Wu, N.; Fang, J. “Preparation and Characterization of Heteropoly-tungstosilicate

Dimers,” Chem. J. Chinese Univ. (Chinese) 1992, 13, 249.

47. Fang, J.; Wu, N.; Wang, Z.; Li, Y. “XPS, AES, and Raman Studies of an Antitarnish Film,”

Corrosion, 1991, 47, 169.

48. Wang, Z.; Wu, N.; Fang, J. “Studies of Heteropolyanions by XPS. II. XPS Study on the

Difference of Thermal Stability between 12-Heterotungstic and Molybdic Acids,” Chemica Acta

Sinica (Chinese) 1991, 49, 788.

49. Fang, J.; Wu, N.; Wang, Z. “Study of Antitarnish Films on Copper by XPS and AES,” Plat.

Surf. Finish. 1990, 77, 54.

50. Wang, Z.; Wu, N.; Fang, J. “Analysis of the Antitarnish Film on a Tin Surface by X-ray

photoelectron Spectroscopy and Auger Electron Spectroscopy,” Analytica Chimica Acta 1990,

238, 399.

51. Wang, Z.; Wu, N.; Fang, J. “Study of Five Heteropolyanions Containing Iron (III),” Chem. J.

Chinese Univ. (Chinese) 1990, 11, 1087.

52. Fang, J.; Wu, N.; Wang, Z. “Determination of the Composition of Viscous Liquid Film on

Electropolishing Copper Surface by XPS and AES,” J. Electrochem. Soc. 1989, 136, 3800.

Presentations 1. David Q. Liu, Naijun Wu, Tackling Sample Matrix Effects in Trace Analysis of Reactive

Genotoxic Impurities by Mass Spectrometry, 66th ASMS Conference on Mass Spectrometry and

Allied Topics, June 3-7, 2018, San Diego Convention Center, San Diego, CA

2. Robert Menger, David Q Liu, Naijun Wu, Method Development Strategies for Potentially

Mutagenic Impurities, North Jersey Chromatography Group Symposium, September 20th, 2017,

Somerset, NJ

3. Robert F. Menger, James E. Winter, Alwyn Forbes, Andy Lo, Peng Wang, Shannon L.

Higgins-Gruber, Ed Bishop, Naijun Wu, Quantitation of Potential Genotoxic Impurities in

Active Pharmaceutical Ingredients, Drug Products, and Intermediates by High Resolution LC-

MS, 64th Annual ASMS Conference on Mass Spectrometry and Allied Topics, June 5-9, 2016,

San Antonio, TX.

4. Naijun Wu, Hon-Wah Man, Overview: High Speed Chromatography to Expedite

Pharmaceutical Development, 7th Annual Shanghai Symposium on Clinical & Pharmaceutical

Solutions through Analysis (CPSA), April 20-23, 2016, Shanghai, China

5. Naijun Wu, (Section Chair), Harmonized Strategy of New UHPLC Implementation in

Pharmaceutical R & D and CRO/CMO QC Laboratories (Section 353), 2015 International

Chemical Congress of Pacific Basin Societies, DECEMBER 15–20, 2015, HONOLULU,

HAWAII

6. Naijun Wu, Advances in UHPLC and HPLC Column Technology, 54th Land O'Lakes

Pharmaceutical Analysis Conference, Madison, WI, USA, August 4-7. 2014

7. Naijun Wu, Xiaolu Liao, Alwyn Forbes, Songling Yu, Theoretical Promises of Ultra-High

Pressure Liquid Chromatography-Comparison with Practical Aspects, Pittcon 2013,

Philadelphia, PA - March 18-22, 2013

8. Naijun Wu, Ashley C. Bradley, Theresa K. Natishan, Invitational Oral Presentation for the

Symposium, "UHPLC in Pharmaceutical Process Development - Comparison with Theoretical

Promise," Pittcon 2011, Atlanta, GA - March 13 -18, 2011.

9. George Zhou, Aaron Moment, Angela Spartalis, Sean Harrington, Shane Grosser, Marguerite

Mohan, Naijun Wu, Ganeshwar Prasad, Tseng-En Hu, Paul Fernandez and John Higgins, "Apply

PAT to the Definition of Design Space to Enable the Control Strategy for Crystallization

Processes," 10th American Institute of Chemical Engineers (AICE) Annual Meeting, No. 355c,

November 7-12, 2010, Salt Lake City, UT.

10. Naijun Wu, Terri Natishan, Invited Oral Presentation, "Fused-Core Particles and Sub-2

Micron Porous Particles for Fast Separation Using HPLC and UPLC in Pharmaceutical

Development," Eastern Analytical 43rd Annual Symposium and Exposition, Somerset, New

Jersey, Nov. 15-18, 2010

11. Wu, Naijun; Li, Zhong; Pascoe, Robert J.; Ma, Guangyu ; Yang, Mei ; Rizos, Panagiota,

Invitational Oral Presentation for the Symposium, "Use of UHPLC for Expediting Method

Development and Difficult Separations," Pittcon 2010, Orlando, Florida, February 28 - March 5,

2010.

12. Naijun Wu; Zhong Li; Rob Pascoe; Guangyu Ma; Monica Yang; Pamela Rizos, "Use of

UHPLC for Expediting Method Development and Difficult Separations," Oral Presentation, 10th

Annual Csaba Horváth Medal Award Symposium, April 14, 2010, Harford Convention Center,

Hartford, CT

13. Naijun Wu, Theresa Natishan, Xiaoyi Gong, Robert Pascoe, Donald Desmond, Christopher

Welch, "Line-of-Sight of Fast HPLC Method Development and Implementation across

Analytical Organizations," Poster Presentation, 2009 PhRMA API WORKSHOP, New

Brunswick, New Jersey, May 19 – 21, 2009.

14. Naijun Wu, Invitational Oral Presentation, "Fast and Efficient LC Separation Using Novel

Column Technology and New Instrumentation" Chromatography Forum of Delaware Valley

2009 Symposium - April 16, 2009 Ft Washington, PA.

15. Naijun Wu, Ahmed Abrahim, Yong Liu, Robert Hartman, Andrew Clausen Invited Talk:

"Application of New HPLC Column Technology – Comparison with Theoretical Promise,"

Eastern Analytical 43rd Annual Symposium and Exposition, Somerset, New Jersey, Nov.16-21,

2008

16. Naijun Wu, Robert Hartman, Yong Liu, and Andrew Clausen, "Performance of Sub-2 µm

Particles at High Pressures in UPLC," HPLC2008, Baltimore, MD, May 10-16, 2008.

17. Naijun Wu, "Fast and Efficient Separations Using Sub-2 µm Particles and Ultra-high

Pressures in Liquid Chromatography," 7th CSSC Csaba Horváth Medal Symposium, Hartford,

Connecticut, April 19-20.

18. Naijun Wu, Invited Seminar: "Fast Separation Using UHPLC," Pace University, Dept. of

Chemistry, 1 Pace Plz, New York, Sept 24, 2007

19. Naijun Wu, Andrew Clausen, Frank Bernardoni, Invited Talk: "Ultra-High Pressure Liquid

Chromatograph for Fast Separations in Pharmaceutical Process Development," (#148) Eastern

Analytical 42nd Annual Symposium and Exposition, Somerset, New Jersey, Nov.12-15, 2007

20. Kate Vogel; Lisa Wright; Andrew Clausen; Naijun Wu "Anaysis of Pharmaceutical

Intermediates Using HPLC with a Porous Graphite Carbon Stationary Phase." (#223), Eastern

Analytical 42nd Annual Symposium and Exposition, Somerset, New Jersey, Nov.12-15, 2007

21. Yadan Chen; Yekaterina Vaynshteyn; Lisa Wright; Naijun Wu; Andrew Clausen; Rositza

Petrova; George Zhou " Identification and Quantification of Amorphous Material in Drug

Substance by ATR-FTIR, Modulated DSC, XRPD, and NIR." (#471). Eastern Analytical 42nd

Annual Symposium and Exposition, Somerset, New Jersey, Nov.12-15, 2007.

22. Lisa Wright; Robert Reamer; Francis Bernardoni, Yadan Chen; Naijun Wu; Andrew

Clausen, "Structure Elucidation Using Preparative Chromatography with Solid-phase Extraction,

MS/MS, H-1 and F-19 NMR for a Drug Substance Process Impurity." (#129), Eastern Analytical

42nd Annual Symposium and Exposition, Somerset, New Jersey, Nov.12-15, 2007

23. Naijun Wu, Invited Talk: "Fast and Efficient Separations Using Sub-2 µm Particles and

Ultra-High Pressures in Liquid Chromatograpy," North Jersey Chromatography Group,

Somerset, NJ, Feb 13, 2007

24. Naijun Wu, Andrew M. Clausen, Lisa J. Wright, and Kate H. Vogel, High-speed Separation

with Increased Efficiency in Support of Pharmaceutical Process Development Using Ultra-

Performance Liquid Chromatograph, Eastern Analytical 42nd Annual Symposium and

Exposition, Somerset, New Jersey, Nov.13-16, 2006

25. Wu, N.; Thompson, R. “Porous and Nonporous Particles for Fast Separation in Packed

Column Chromatography” The Pittcon 2005, Oral Presentation, Orlando, FL, Feb.26 - March 4,

2005.

26. Wu, N.; Yehl, P.M.; Dovletoglou, A. Fast HPLC Separation Using 1.5 µm Porous and Non-

porous Particles. Eastern Analytical 42nd Annual Symposium and Exposition, Somerset, NJ,

Nov., 2003

27. Wu, N.; Xiang, Y.; Alonso, M.C.; Barceló, D.B.; Collins, D.C.; Lippert, A.J.; Lee, M.L.

“Utilization of Very Small Particles and Ultra-high Pressures for High Throughput Analysis”

The Pittcon 2001, Oral Presentation, New Orleans, LA, March, 2001, No. 842.

28. Xiang, Y.; Lippert, A.J.; Wu, N.; Lee, M.L. “Separation of Chiral Pharmaceuticals Using

Ultra-high Pressures Liquid Chromatography” The Pittcon 2001, Oral Presentation, New

Orleans, LA, March, 2001, No. 569.

29. Xiang, Y.; Wu, N.; Lippert, A.J.; Lee, M.L. “Separation of Chiral Pharmaceuticals Using

UHPLC” The 24th International Symposium on Capillary Chromatography, Las Vegas, Nevada,

May 20-24, 2001.

30. Lee, M.L.; Wu, N.; Lippert, A.J.; Tang, Q.; Bowerbank, C.R.; Shen, Y. “Utilizing Elevated

Pressure in Microcolumn Separations,” Oral Presentation, The Pittcon 2000, New Orleans, LA,

March, 2000, No. 659.

31. Wu, N.; Tang, Q.; Lippert, A.J.; Lee, M.L. “Solvating Gas Chromatography Using Water as

Mobile Phase,” The Pittcon 2000, Oral Presentation, New Orleans, LA, March, 2000, No. 914.

32. Tang, Q.; Wu, N.; Bowerbank, C.R.; Shen, Y.; Lee, M.L. “So-gel Bonded Continuous-bed

Columns for Nono Column Separation,” The Pittston 2000, Oral Presentation, New Orleans, LA,

March, 2000, No. 1113.

33. Lee, M.L.; Wu, N.; Lippert, A.J.; Xin, B.“Practical Aspects of Ultrahigh-Pressure Liquid

Chromatography,” HPLC’2000: 24th International Symposium & Exhibit on High Performance

Liquid Phase Separations & Related Techniques, Oral Presentation, June 24-30, 2000, Seattle,

Washington, L-0405.

34. Wu, N.; Chen, Z.; Medina, J.C.; Bradshaw, J.S. Lee, M.L. “Fast Separations of Enantiomers

Using Near Critical Carbon Dioxide,” The 23rd International Symposium on Capillary

Chromatography, Riva del Garda, Italy, June 5-10, 2000.

35. Medina, J.C.; Wu, N.; Lee, M.L. “Comparison of Empirical Peak Capacities for High-

efficiency Capillary Chromatographic Techniques,” The 23rd International Symposium on

Capillary Chromatography, Riva del Garda, Italy, June 5-10, 2000.

36. Wu, N.; Lee, M.L. “Compressible Mobile Phases in Packed Capillary Chromatography,” The

42nd Rocky Mountain Conference on Analytical Chemistry, Invited Talk, Broomfield, Colorado,

July 30-August 3, 2000, No. 295.

37. Wu, N.; Tang, Q.; Lee, M.L. “SGC Using Neat Water as Mobile Phase,” ACS Joint 55th

Northwest/16th Rocky Mountain Regional Meeting, Oral Presentation, Idaho Falls, Idaho, June

15-17,

2000.

38. Collins, D.C.; Tang, Q.; Wu, N.; Lee, M.L. “Fast Liquid Chromatography-Time-of-Flight

Mass Spectrometry Using Sol-gel Bonded Continuous-Bed Capillary Columns.” ACS Joint 55th

Northwest/16th Rocky Mountain Regional Meeting, Oral Presentation, Idaho Falls, Idaho, June

15-17, 2000.

39. Medina, J.C.; Wu, N.; Lee, M.L. “Study of Peak Capacities in Capillary Chromatographic

Techniques,” ACS Joint 55th Northwest/16th Rocky Mountain Regional Meeting, Oral

Presentation, Idaho Falls, Idaho, June 15-17, 2000.

40. Wu, N.; Collins, D.C.; Lippert, A.J.; Xiang, Y.; Lee, M.L. “Ultrahigh-Pressure Liquid

Chromatography/Time-of-Flight Mass Spectroscopy for Fast Separations” 23rd International

Symposium on Chromatography, Olympia II, London, UK, October 2-3.

41. Wu, N.; Lee, M.L. “Porous and Nonporous Silica Particles in Solvating Gas

Chromatography,” 21st International Symposium on Capillary Chromatography and

Electrophoresis, Park City, UT, June 20-24, 1999, p.195.

42. Shen, Y.; Wu, N.; Tang, Q.; Chen, Z.; Bradshaw, J.S.; Lee, M.L. “Effect of Pore Size on

Speed of Chiral Separations Using Packed Capillary SFC,” 21st International Symposium on

Capillary Chromatography and Electrophoresis, Park City, UT, June 20-24, 1999, p.193.

43. Tang, Q.; Wu, N.; Lee, M.L. “Capillary Liquid Chromatography Using So-gel Bonded

Continuous Bed Columns,” 21st International Symposium on Capillary Chromatography and

Electrophoresis, Park City, UT, June 20-24, 1999, p.128.

44. Lee, M.L.; Xin, B.; Lipert, J.A.; Tang, Q.; Bowerbank, C.R.; Wu, N.; Shen, Y.

“Instrumentation and Column Technology for Fast Condensed Phase Microcolumn Separation

Techniques,” 38th Annual Eastern Analytical Symposium & Exposition, Oral Presentation,

Somerset, New Jersey, November 14-19, 1998.

45. Wu, N.; Shen, Y.; Lee, M.L. “Comparison of SFC and Solvating Gas Chromatography,” 19th

International Symposium on Capillary Chromatography and Electrophoresis, Wintergreen, VA,

May 18-22, 1997.

46. Wu, N.; Chen, Z.; Wang, Z. “Determination of Trace Iron in a Large Quantity of Copper by

Mix-masking Methods,” The 45th Pittsburgh Conference, Chicago, IL, Feb. 27-March 4, 1994,

145p.

47. Wu, N.; Liang, J.; Wang, Z. “Synthesis and Characterization of Keggin Heteropoly Acids,”

The 45th Pittsburgh Conference, Chicago, IL, Feb. 27-March 4, 1994, 285p.

Seminar Series Coordinator:

Yuanwei Zhang