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University of Colorado

RESIDENT RESEARCH DAY June 8, 2012

Department of Otolaryngology

Welcome to the Department of Otolaryngology Resident Research Day at the University of Colorado School of Medicine. The major educational function of the Department is to train the complete otolaryngologist – head and neck surgeon. Under supervision of the medical staff, residents participate in all phases of patient care, education, and research.

Patient care discussions go on every day. However, today the residents will have the opportunity to present their scholarly research activities to the group. We are aware that most are not going to do hands on research in the future, but you must be consumers of research in your daily practice. Developing critical judgment promotes better patient care. Understanding the basic scientific method principles is the first step. Most of the projects today were begun in the 2nd year of residency and completed over the course of the training.

Thank you for participating in our program today and we look forward to seeing everyone in June 2013 for the 6th Annual Resident Research Day.

Special ThanksWe would like to acknowledge our educational grant sponsor for our Annual Resident Research Day. We appreciate their continued support and commitment towards our resident educational endeavors.

Resident Research Day 2012 – Welcome

Department of Otolaryngology Resident Research Day June 8, 2012 Welcome 2

University of Colorado School of MedicineDepartment of Otolaryngology

welcome

7:30AM Valérie Côté MD, CM, FRCS(C): Characteristics of Children Under 2 Years of Age with Obstructive Sleep Apnea

7:45AM Pamela A. Mudd MD: Postoperative Management in Laryngotracheal Reconstruction; Report of Sedation Outcomes in Varied Post Operative Management

8:00AM Adam M. Holdt MD: Glomus Jugulare Tumors and Stereotactic Radiosurgery: Tumor Control and Hearing Preservation Over Time

8:15AM Jacob S. Minor MD: Methylation of MicroRNA-9 is a Specific and Sensitive Biomarker for Oral and Oropharyngeal Squamous Cell Carcinomas

8:30AM Matthew J. Whinery MD: Methylation of MicroRNA-124 is a Novel Biomarker for HNSCCs

8:45AM BREAK & RESIDENT GROUP PHOTOS

9:00AM Justin M. Wudel MD: Synthetic Hydrogel Scaffold is an Effective Vehicle for Delivery of rhBMP-2 (INFUSE®) to Critical-Sized Calvarial Bone Defects in Rats

9:15AM J. Eric Lupo MD, MS: Active Middle Ear Implant Third Window Vibroplasty: Electrocochleographic Assessment of Direct Mechanical Cochlear Stimulation in Experimentally-Induced Oval and Round Window Fixation

9:30AM Henry P. Barham MD: Investigation into Nasal Solitary Chemoreceptor Cells

9:45AM Sarah E. Cooper MD: Expression of Taste Receptors in Human Sinonasal Mucosa

10:00AM Scott E. Mann MD: Inhibition of K+ Currents in Type I Vestibular Hair Cells by Aminoglycosides

10:15AM BREAK

10:30AM Gary McDonald MD: Chiefs Special Presentation in Memory of Dr. Clayton Mammel

10:35-11:15AM Robin T. Cotton MD, FACS, FRCS(C) Professor, Department of Otolaryngology, University of Cincinnati College of Medicine Current Management of Pediatric Airway

11:15AM Geoffrey R. Ferril MD: Correlation of Upper and Lower Airway Acid-Fast Bacilli Cultures in Immunocompetent Adults with Nontuberculous Mycobacterial Bronchiectasis

11:30AM Brook K. McConnell MD: Nasal Septal Perforation Associated with Pyoderma Gangrenosum

11:45AM Katherine K. Green MD, MS: Oropharyngeal Tularemia Presenting with Pharyngitis and a Neck Mass

12:00PM Closing Remarks & Transition to Poster Display Q & A Session 2nd Floor Research Complex Bldg 2 Poster Display in Trivisible Atrium

12:30PM LUNCH

1:30-3:30PM Visiting Professor Rounds with Robin T. Cotton MD, FACS, FRCS(C)

Department of Otolaryngology Resident Research Day June 8, 2012 Schedule 3

Resident Research Day 2012 – Schedule


schedule

Robin T. Cotton MD, FACS, FRCS(C)Professor, Department of Otolaryngology- Head and Neck Surgery University of Cincinnati College of Medicine Director, Department of Pediatric Otolaryngology, Children’s Hospital Medical Center, Cincinnati, OH

Originally from England, Robin T. Cotton, MD, director of the Department of Pediatric Otolaryngology – Head and Neck Surgery is a graduate of the University of Cambridge, Cambridge, England and of the University of Birmingham, Birmingham, England. His Otolaryngology residency and fellowship at the University of Toronto was followed by a Head & Neck Fellowship at the University of Cincinnati.

In 1973 he became director of the Department of Pediatric Otolaryngology -- Head and Neck Surgery at Cincinnati Children’s Hospital Medical Center. He is an accomplished author – publishing hundreds of articles and many books, chapters and monographs. Dr. Cotton is a member of multiple societies and committees, has held numerous offices over the course of his career and has received a long list of awards and recognitions for his accomplishments.

Dr. Cotton has built the world’s premiere center for the diagnosis and treatment of airway abnormalities. He was instrumental in developing the Airway Management Unit, which has evolved into the Aerodigestive Sleep Center. The Aerodigestive Sleep Center is now the forefront of the multidisciplinary approach management for children with complex airway disorders.

The Department of Pediatric Otolaryngology has become the busiest surgical subspeciality at Cincinnati Children’s, with more than 36,000 visits and 15,000 surgeries each year. The Pediatric Otolaryngology -- Head and Neck Surgery Division was the first to utilize Cincinnati Children’s outpatient facilities.

Dr. Cotton has been an invited speaker to many national and international functions each year. Because of his reputation, 70 percent of his patients requiring airway surgery are from outside the Ohio, Indiana and Kentucky area.

Together, Dr. Cotton and his colleagues have developed a world-renowned program to treat primary and specialized ear, nose and throat problems, including swallowing disorders, speech disorders and cochlear implants.

Named one of the “Best Doctors in the United States” in 1998, Dr. Cotton and his staff have built an international reputation for the treatment of hearing and aerodigestive conditions in children.

Department of Otolaryngology Resident Research Day June 8, 2012 Robin T. Cotton MD, FACS, FRCS(C) 4

Resident Research Day 2012 – Keynote Speaker


keynote

Sue C. Kinnamon PhDProfessorDepartment of Otolaryngology Research Division, Department of Cell & Developmental Biology, and Department of Physiology & Biophysics

Dr. Kinnamon received her PhD from Kansas State University and completed postdoctoral fellowships at the University of Colorado Health Sciences Center. In 1985 she joined the faculty at Colorado State University, Department of Biomedical Sciences. In January, 2009, she became a tenured Professor at the UC Department of Otolaryngol-ogy, with joint appointments in Physiology & Biophysics and Cell & Developmental Biology. She is also a member of the Neuroscience Graduate Faculty at UC Denver.

Research Interests: Dr. Kinnamon’s research focuses on the gustatory system and the common chemi-cal sense. We are interested in how taste cells detect chemical stimuli, how stimuli are transduced into receptor potentials, and how this information is transmitted to gustatory afferent fibers. Studies on the common chemical sense include the function of solitary chemoreceptor cells in the nasal respiratory epithelium. These cells are innervated by the trigeminal nerve and detect noxious chemicals and the presence of bacteria in the airways. Activation of these cells provokes protective airway reflexes. Current studies are focusing on their role in human nasal tissue.

Publications:Tizzano, M., Gulbransen, B.D., Vandenbeuch, A., Clapp, T.R., Herman, J.P., Sibhatu, H.M., Churchill, M.E.A., Silver, W.L., Kinnamon, S.C., and Finger, T.E. (2009). Nasal che-mosensory cells utilize bitter taste signalling to detect irritants and pathogenic Bacteria. PNAS 107:3210-5.

Vandenbeuch, A., Tizzano, M., Anderson, C.B., Stone, L.M., Goldberg, D. and Kin-namon, S.C. (2010). Evidence for a role of glutamate as an efferent transmitter in taste buds.BMC Neurosci. 2010 Jun 21;11:77.

Vandenbeuch, A., Zorec, R., and Kinnamon, S.C. (2010). Capacitance measurements of regulated exocytosis in mouse taste cells. J. Neuroscience, 30(44):14695-701.

Awards:Dr. Kinnamon has been continuously funded by the NIH since 1987. Currently she has an R21 from NIDCD to study mechanisms of taste transduction & signaling, is PI on a subcontract from the University of Miami to study sweet taste, and is Co-PI with Dr. Tom Finger on the role of solitary chemoreceptor cells in the dection of pathogenic bacteria. Dr. Kinnamon has served on NIH and NSF study sections and was awarded the Associa-tion for Chemoreception Sciences Award for Outstanding Achievement in 2001. She is currently serving on two editorial boards and is a member of Faculty of 1000.

Resident Research Day 2012 – Faculty

Department of Otolaryngology Resident Research Day June 8, 2012 Sue C. Kinnamon PhD 5


faculty

Shi-Long Lu MD, PhDAssistant ProfessorDepartment of Otolaryngology Research Division, Department of Dermatology, and Department of Pathology

Dr. Lu received his PhD from Tokyo Medical and Dental University, and his MD from China Medical University. He completed his post-doctoral training in the Howard Hughes Medical Institute Lab at the Case Western Reserve University. He was a Research Assistant Professor of Otolaryngology and Dermatology at the Oregon Health & Science University. He is currently an Assistant Professor of Otolaryngology, Dermatology, Pathology, and a member of the Cancer Biology Graduate Faculty, and the Cell and Developmental Biology at UCD. He is also a faculty member of the Head and Neck Cancer Research Program, and the Regenerative Medicine & Stem Cell Biology Program.

Research Interests: Dr. Lu’s research interest is on head and neck squamous cell carcinomas. The goal is to understand the molecular mechanisms underlying the development and progression of HNSCC. It is hoped that these studies will lead to identification of novel molecular markers for future clinical diagnosis and experimental therapy.

Publications:Bornstein S, White R, Malkoski S, Oka M, Han G, Cleaver T, Reh D, Andersen P, Gross N, Olson S, Deng C, Lu SL, Wang XJ. Smad4 loss in mice causes spontaneous head and neck cancer with increased genomic instability and inflammation. J Clin Invest 119: 3408-19 (2009)

Lu SL, Herrington H, Reh D, Weber S, Bornstein S, Wang D, Tang CF, Siddiqui Y, Nord J, Andersen P, Corless C, Wang XJ: Loss of Transforming Growth Factor-beta Type Ii Receptor Promotes Metastatic Head-and-neck Squamous Cell Carcinoma. Genes Dev. 20: 1331-1342 (2006).

Lu SL, Reh D, Li AG, Woods J, Corless CL, Kulesz-Martin M, Wang XJ: Overexpression of Transforming Growth Factor Beta1 in Head and Neck Epithelia Results in Inflammation, Angiogenesis, and Epithelial Hyperproliferation. Cancer Res. 64: 4405-4410 (2004).

Awards:Dr. Lu is currently funded by a R01 from NIDCR to study the role of PI3K pathway in head and neck cancer invasion and metastasis. Dr. Lu has also received grant awards from the American Cancer Society; the Thyroid, Head and Neck Cancer (THANC) Foundation; the Dermatology Foundation; Cancer League of Colorado; and the Oregon Medical Foundation. He is also a recipient of a Research Career Development Award from the Dermatology Foundation.


Department of Otolaryngology Resident Research Day June 8, 2012 Shi-Long Lu MD, PhD 6


faculty

Katherine J. Rennie PhDAssociate Professor & Director Department of Otolaryngology Research Division, and Department of Physiology & Biophysics

Dr. Rennie received her PhD from the University of Bristol, Avon, U.K. and completed a post-doctoral fellowship in the Department of Otolaryngology at the University of Texas Medical Branch in Galveston. She is currently an Associate Professor of Otolaryngology, Physiology & Biophysics, and a member of the Neuroscience Graduate Faculty at the University of Colorado.

Research Interests: My research is focused on the peripheral vestibular system. The goal is to identify ion channels present in vestibular cells in order to understand how signals are transformed from a mechanical stimulus at the hair cell bundle into electrical activity of the primary vestibular neurons. Electrophysiological (patch clamp), molecular and mathematical modeling approaches are used. We have identified several different types of potassium channels in vestibular cells and are currently assessing synaptic mechanisms. It is hoped that results from these studies will clarify some of the mechanisms underlying vestibular disorders.

Publications:Rennie, K. J. and Streeter, M. A. Voltage-dependent currents in isolated vestibular afferent ca-lyx terminals. J. Neurophysiol. 95:26-32, 2006.

Li, G., Meredith, F. L. and Rennie, K. J. Development of K+ and Na+ conductances in rodent postnatal semicircular canal type I hair cells. Am. J. Physiol. Regul. Integr. Comp. Physiol. 298: R351-R358, 2010. PMCID2828173.

Dhawan, R., Mann, S. E., Meredith, F. L. and Rennie, K. J. K+ currents in isolated vestibular af-ferentcalyx terminals. J Assoc Res Otolaryngol 11:463-476, 2010. PMCID2914245.

Meredith, F. L. Li, G., and Rennie, K. J. Postnatal expression of an apamin-sensitive K(Ca) cur-rent in vestibular calyx terminals J. Membr. Biol. 244:81-91, 2011.

Awards: Dr. Rennie has received several grant awards from the NIH (NRSA postdoctoral fellowship, R03 & R29 FIRST Award) and is currently funded by the American Otological Society. She has also received funding from the Deafness Research Foundation, the American Hearing Research Foundation and the National Organization for Hearing Research Foundation and is a former re-cipient of a Research Career Enhancement Award from the American Physiological Society. Dr. Rennie has reviewed grants for the NIDCD (NIH), National Science Foundation, Wellcome Trust, RNID and Deafness Research Foundation and reviewed articles for Acta Pharmacologica Sinica, American Journal of Physiology-Cell Physiology, Experimental Neurology, JARO, Journal of Comparative Neurology, Journal of Neurophysiology, Journal of Neuroscience, Journal of Physiology, Journal of Vestibular Research and Neurochemical Research.


Department of Otolaryngology Resident Research Day June 8, 2012 Katherine J. Rennie PhD 7


faculty

Department of Otolaryngology Resident Research Day June 8, 2012 Kristin M. Uhler PhD 8

Kristin M. Uhler PhDAssistant Professor Department of Otolaryngology

Dr. Uhler received her PhD in 2008 from the University of Colorado, Boulder. She joined the Department of Otolaryngology in 2009 as an Assistant Professor of Otolaryngology, University of Colorado, Denver.

Research Interests:Infant speech perception in deaf and hard-of-hearing children and the implantable hear-ing devices for listeners of all ages and their impact on speech understanding abilities.

Publications:Jenkins, H.A., Uhler, K., Lupo, V., & Bell-Lehmkuhler, B. (2009). Otologics Middle Ear System Phase IIb Clinical Trial: Preliminary Results. Proceedings of the 7th Asia Pacific Symposium on Cochlear Implants and Related Sciences (APSCI), 51-56 .

Uhler, K, Burns, S, Dalpes, M. & Yoshinaga-Itano, C (2011) An Auditory Spoken Lan-guage Matrix for Differential Diagnosis of Spanish-speaking Children Who are Deaf or Hard of Hearing, Special interest group 14, communication disorders and sciences in culturally and linguistically diverse populations

Uhler K, Yoshinaga-Itano C, Gabbard SA, Rothpletz A, Jenkins HA: Longitudinal Infant Speech Perception in Young Cochlear Implant Users. J Amer Acad Audiol, 2011

Jenkins HA, Uhler K, Lupo V, Bell-Lehmkuhler B: (2009) Otologics Middle Ear, 51-56. System Phase IIb Clinical Trial: Preliminary Results. Proceedings of the 7th Asia Pacific Symposium on Cochlear Implants and Related Sciences (APSCI)

Presentations:Uhler K, Bell-Lehmkuhler B, Lupo VK, Jenkins HA, Claycomb S, Gabbard SA, Sedey A: (2010) One-year speech perception outcomes for a fully implantable middle ear device. Presentation at the American Academy of Audiology, April, 2010.

Uhler KM, Yoshinaga-Itano C, Gabbard S, Rothpletz A, Jenkins H: (2010) Longitudinal Study of Infant Speech Perception in Young Cochlear Implant Candidates: Three Case Studies. Presentation at the Auditory Society, March, 2010.

Uhler K, Yoshinaga-Itano C, Gabbard S, Fredrickson T: (2008) Longitudinal Study of Infant Speech Perception in Young Cochlear Implant Candidates: Three Case Studies. Presentation at the 10th Annual Cochlear Implant Symposium, April, 2008.



faculty

Aurelie Vandenbeuch PhDInstructorDepartment of Otolaryngology Research Division

Dr. Vandenbeuch received her PhD from University of Paris, Denis Diderot in 2006. She completed a postdoctoral fellowship at Colorado State University (2006-2009) and at the University of Colorado Denver (2009-2011). In January 2011, she was appointed Instructor in the Department of Otolaryngology and is currently working in Sue Kinna-mon’s laboratory.

Research Interests: Her research interests are dedicated to the neurobiology of the chemical senses and more particularly on the taste system. Working in Sue Kinnamon’s lab, she is interested in the different transduction mechanisms that allow the gustatory information to be pro-cessed: from a molecule binding to its receptor on the tongue epithelium to the trans-mission to the central nervous system via the gustatory nerve fibers. Her attention is more focused on the peripheral system and how taste bud cells communicate with the afferent nerve fibers to transmit the information to the brain.

Publications: Vandenbeuch A., Zorec R, Kinnamon S. Capacitance measurements of regulated exo-cytosis in mouse taste cells (2010). J Neurosci. 3;30(44):14695-701.

Vandenbeuch A, Tizzano M., Anderson C., Stone L., Goldberg D, Kinnamon S. Evi-dence for a role of glutamate as an efferent transmitter in taste buds (2010). BMC Neu-rosci. 21;11:77.

Tizzano M, Gulbransen BD, Vandenbeuch A, Clapp TR, Herman JP, Sibhatu HM, Churchill ME, Silver WL, Kinnamon SC, Finger TE. Nasal chemosensory cells use bitter taste signaling to detect irritants and bacterial signals (2010). Proc Natl Acad Sci U S A. Feb 16;107(7):3210-5.

Vandenbeuch A, Kinnamon SC. Why do taste cells generate action potentials? (2009). J Biol.;8(4):42.

Awards: Dr. Vandenbeuch received the Polak Young Investigator award in 2008 from the Asso-ciation for Chemical Senses (AChemS).

Department of Otolaryngology Resident Research Day June 8, 2012 Aurelie Vandenbeuch PhD 9



faculty

2012 Resident Research DayPanel of Judges

Mona M. Abaza MD, MSAssociate Professor & Program Director Kenny H. Chan MDProfessor

Vincent D. Eusterman MD, DDSAssociate Professor

Kristin M. Uhler PhDAssistant Professor Robin T. Cotton MD, FACS, FRCS(C)ProfessorDepartment of Otolaryngology- Head and Neck SurgeryUniversity of Cincinnati College of Medicine Director, Department of Pediatric Otolaryngology, Children’s Hospital Medical Center

Awards There will be a 1st and 2nd place award given for “Outstanding Resident Research Presentation” for best demonstration of either basic science or clinical research.

The criteria for selection will be based upon the significance of the research finding to the field of Otolaryngology, the quality of work performed and the quality of presentation.

Resident Research Day 2012 – Judges Panel & Awards

Department of Otolaryngology Resident Research Day June 8, 2012 Judges Panel and Awards 10


judges & awards

Objectives: To study characteristics of children under 2 years who underwent a tonsillectomy after having a polysomnogram (PSG). To identify predictors of severe obstructive sleep apnea (OSA) in this age group.

Design: Retrospective chart review.

Setting: Tertiary care pediatric hospital.

Patients: Children under 2 years of age who un-derwent a PSG prior to tonsillectomy over a 7-year period.

Methods: Race, weight-for-age percentile, prema-turity, smoking exposure, daycare, pres-ence of syndromes, craniofacial anoma-lies, or cerebral palsy, and preoperative oxygen use were collected. Children were stratified by OSA severity using two separate scales: the obstructive apnea-hypopnea index (OAHI) and the OAHI plus oxygen nadir (OAHI+nadir).

Results: 225 (2.5%) of 9,038 patients who under-went tonsillectomy were under 2 years of age. Of these, 72 patients (32.0 %) had preoperative PSGs. The median age was 20.4 months (range: 10.6-23.9). The pro-portion of premature patients (26.4%) in our cohort was significantly different than

the proportion of the general population in Colorado (9.1%; p<0.05).

Univariate association tests revealed a significant association between OSA severity and ethnicity only (p=0.02). In a multivariate model for the OAHI scale, African-Americans were 11 times more likely than Caucasians to get severe OSA according to the OAHI scale. There was no difference between African-Americans and Latin-Americans. Using the OAHI scale, 36 patients (50.7%) had severe OSA, while 54 patients (76.1%) were classified in the severe OSA using the OAHI+nadir scale. In a multivariate model for the OAHI+nadir scale, no sig-nificant predictors were found. The OAHI and OAHI+nadir scales had less than mediocre agreement (kappa=0.5) for de-fining OSA severity. Conclusion: Patient ethnicity seems to be a predictor of OSA severity in children under 2 years of age undergoing PSG. The OAHI scale for OSA also might be more adequate for patients under 2 years of age, especially if they live at higher altitudes and have more frequently low oxygen nadirs.

Characteristics of Children Under 2 Years of Age with Obstructive Sleep Apnea

Department of Otolaryngology Resident Research Day June 8, 2012 Valérie Côté MD, CM, FRCS(C) 11

Valérie Côté MD, CM, FRCS(C)1,2, Conan Liang1,2, Jonathan N. Perkins1,2, Stefan Sillau MS3, Norman Friedman MD1,2

1University of Colorado School of Medicine, Department of Otolaryngology 2Children’s Hospital Colorado, Division of Pediatric Otolaryngology, Aurora, CO 3University of Colorado School of Public Health, Aurora, CO

Valé

rie C

ôté

MD,

CM

, FRC

S(C)

Clinical

Objective:To report sedation related outcomes in varied postoperative approach after single-stage laryngotracheal reconstruction in children.

Design: Retrospective study.

Setting: Tertiary care pediatric hospital.

Patients: Thirty three children who underwent sin-gle-stage laryngotracheal reconstruction, excluding anterior cricoid split, tracheal resection, and cricotracheal resection.

Interventions: Ten-year period of data collection regarding postoperative care and complications.

Results: The medical records of all patients who underwent single-stage laryngotracheoplasty between 2001 and 2011 were reviewed.Tracheally intubated, minimally sedated, and awake patients (n=15) were compared with tracheally intubated, sedated, and medically paralyzed patients (n=20). The use of opioids, benzodiazepine, and precedex on either an as needed or continuous basis was recorded. Outcome measures examined as correlating to post operative sedation

management included: total length of stay in ICU and hospital, nursing concerns, symptomatic atelectasis, accidental decannulation, requirement for additional surgical procedures including need for revision, and importantly incidence of associated withdrawal symptoms.

Conclusion: Postoperative management after single-stage laryngotracheal reconstruction does not require the use of physical and pharmacologic restraints. Children who are awake and comfortable without medical restraint do not experience withdrawal symptoms, do not require adjunctive medications for withdrawal, and have fewer associated pulmonary complications. Five patients required tracheostomy and/or revision larngotracheoplasty, four of whom had been medically paralyzed and one who was managed with minimal short term sedation. Nine patients required adjunctive balloon dilation, five of whom had been medically paralyzed and four who were managed without sedation.

Postoperative Management in Laryngotracheal Reconstruction; Report of Sedation Related Outcomes in Varied Postoperative Management

Department of Otolaryngology Resident Research Day June 8, 2012 Pamela A. Mudd MD 12

Pamela Mudd MD1, Matthew Powers, Peggy Kelley MD1,2

1University of Colorado School of Medicine, Department of Otolaryngology2Children’s Hospital Colorado, Aurora, CO

Pam

ela

A. M

udd

MD

Clinical

Background: Glomus jugulare tumors (GJT) are tradi-tionally treated surgically, but select tu-mors can be treated by radiation therapy based on location and patient factors. Recently, stereotactic radiosurgery (SRS) has been shown to be an effective treat-ment modality for GJT control, providing a more limited radiation dose to critical adjacent skull base structures compared with more traditional radiation therapies. This study retrospectively examines the rate of tumor control and hearing preser-vation in GJT patients after SRS.

Method: This retrospective study reviewed all nineteen adult GJT patients treated by SRS at the University of Colorado Hospi-tal between 2003 and 2012. Primary out-come was hearing loss. Secondary out-comes included radiologic progression or treatment failure, other cranial nerve deficits, and treatment adverse effects.

Results: The current SRS protocol at the Uni-versity of Colorado provides excellent radiologic tumor stabilization. Significant decrease in AAO-HNS hearing class was not seen in these patients.

Conclusion: SRS treatment of GJT’s is a viable treat-ment modality as an alternative to surgi-cal resection in selected patients.

Glomus Jugulare Tumors and Stereotactic Radiosurgery: Tumor Control and Hearing Preservation Over Time

Department of Otolaryngology Resident Research Day June 8, 2012 Adam M. Holdt MD 13

Adam Holdt MD, Scott Mann MD, Stephen Cass MD, MPH

University of Colorado School of Medicine, Department of Otolaryngology

Adam

M. H

oldt

MD

Clinical

Introduction: Detection of DNA methylation has pro-duced promising results as biomarkers for head and neck squamous cell carci-noma (HNSCC). However, current panels are limited by an insufficient number of sensitive and specific tumor markers. MicroRNAs (miR) play an important role in tumorigenesis, and may represent a novel panel of molecules for the devel-opment of cancer biomarkers.

Methods: We investigated methylation of three miRNA promoter sites of miR-9 (miR-9-1,miR-9-2, miR-9-3) in 107 human head and neck tissue samples and controls. Total RNA, including MicroRNA, was extracted from tissue samples. DNA was also extracted and treated with a bisul-fate protocol to allow for performance of methylation-specific PCR. MicroRNA ex-pression was quantitated with real-time PCR, and methylation-specific PCR was performed in promoter regions.

Results: We found methylations of miR-9-1 and miR-9-3 were higher in oral and oropha-ryngeal carcinomas than that in laryn-geal carcinoma, achieving a combined sensitivity of 63% and 56%, respectively, for these two tumor types, compared to 21% for the laryngeal carcinoma. Quan-titative PCR of miR-9 showed reduced expression associated with methylation

of miR-9 in tumor tissues. To investigate the functional consequences of miR-9 methylation, we found that miR-9 meth-ylation is correlated with miR-9 expres-sion level in human HNSCC cell lines. Demethylation treatment using 5-azad-eoxycytidine restored its expression in a miR-9 methylated human HNSCC cell line UMSCC22A. Furthermore, cell pro-liferation and viability was significantly inhibited, while PTEN expression was el-evated after transfection of miR-9 into the UM-SCC22A cell line.

Conclusion: Our results suggest that methylations of miR-9-1 and miR-9-3 are sensitive and specific biomarkers for HNSCC, particularly for oral and oropharyngeal squamous cell carcinomas. In addition, miR-9 may function as a tumor suppres-sor in HNSCC through inhibition of cell proliferation and elevation of tumor sup-pressor PTEN.

Methylation of MicroRNA-9 is a Specific and Sensitive Biomarker for Oral Oropharyngeal Squamous Cell Carcinomas

Department of Otolaryngology Resident Research Day June 8, 2012 Jacob S. Minor MD 14

Jacob Minor MD1, Xiaotian Wang1, Fang Zhang MD1, John Song MD1, Antonio Jimeno MD1, PhD, Xiao-Jing Wang MD, PhD2, Xian Lu3, Neil Gross MD5, Molly Kulesz-Martin PhD5, Daren Wang MD, PhD1, Shi-Long Lu MD, PhD1

University of Colorado School of Medicine, 1Department of Otolaryngology 2Department of Pathology, 3Department of Bioinformatics University of Portland-Oregon Health & Science, 4Department of Otolaryngology, 5Department of Dermatology

Jaco

b S.

Min

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D

Basic Science

Introduction:MicroRNAs, initially discovered in 1993 in the organism C. elegan, have be-come the focus of significant research in the last decade. These tiny RNA par-ticles, only 15 to 25 nucleotides in length, are highly conserved across species and highly specific to tissue and devel-opmental stage. Studies have demon-strated both over and under-expression of a variety of miRNAs in both solid and hematological malignancies. As miRNA expression patterns are tissue specific, they are potentially useful for identifying the progenitor cell line in highly undif-ferentiated cancers and with unknown primaries. Both genetic and epigenetic alterations can cause over/under tran-scription of miRNA and result in tumor-genesis. We chose to investigate miRNA under expression in Head and Neck Squamous Cell Carcinoma (HNSCC).

Materials/Methods:We first extracted genomic DNA from our cell lines – 8 “normal/control” lines and 15 HNSCC cell lines. The genomic DNA underwent bisulfite conversion al-lowing us to perform methylation specific PCR (MS-PCR) using methylated and unmethylated miRNA primers. Those miRNA methylated primers that showed cancer cell specific signals became our candidate miRNAs. Each candidate was then tested upon bisulfate treated ge-nomic DNA obtained from actual HNSCC samples, adjacent tissue, and control

tissue again looking for hypermethylated miRNA specific to HNSCC tissue. Re-sults were then statistically analyzed.

Results:Our study identified three new promising miRNA markers for HNSCC in addition to two markers that our lab had previously identified. miR 124-1, 124-2, 124-3 had a combined sensitivity and specificity of 83.3% and 100% respectively. With the addition of miR 9-1 & 9-3, markers previ-ously identified in our lab, the sensitivity increased to 88.1% while specificity (as controlled by our cut-off values) re-mained 100%. While not statistically sig-nificant, the markers had a trend toward the greatest sensitivity with oropharygeal HNSCC.

Conclusion:Our panel of 5 miRNA markers provides 88.1% sensitivity and 100% specificity as markers for HNSCC in our tumor bank. These results are significantly better than previously reported markers for HNSCC and provide exciting potential detection and treatment applications of miRNA with HNSCC.

Methylation of MicroRNA-124 is a Novel Biomarker for HNSCCs

Department of Otolaryngology Resident Research Day June 8, 2012 Matthew J. Whinery MD 15

Matthew Whinery MD1, John Song MD1, Antonio Jimeno MD, PhD1, Neil Gross MD2, Molly Kulesz-Martin PhD3, Shi-Long Lu MD, PhD1

1University of Colorado School of Medicine, Department of Otolaryngology Oregon Health & Science University, 2Department of Otolaryngology, 3Department of Dermatology

Mat

thew

J. W

hine

ry M

D

Basic Science

Objective:To determine whether radical-mediated, thiol-ene chemistry can be used to cre-ate an effective polymer delivery vehicle for rhBMP-2, eliminating the use of xenografic materials and reducing the rhBMP-2 dose required to achieve thera-peutic effects.

Methods:Critical sized cranial defects (8mm) were created in 12-week-old male Sprague Dawley rats. The rats were randomly assigned to receive no treatment, ab-sorbable collagen sponge (ACS), poly-ethylene glycol hydrogel (PEG), 0.2mg of bone morphogenic protein (rhBMP-2) + ACS, 2mg rhBMP-2 + ACS, 0.2mg rh-BMP-2 + PEG, or 2mg rhBMP-2 + PEG. Micro CT scans were completed at 0, 2, and 6 weeks and three dimensional volu-metric measures were used to track bone growth. At 6 weeks the rats were sac-rificed and the calvaria were removed, sectioned, and stained for histologic evaluation.

Results:PEG delivery of the rhBMP-2 resulted in significantly faster defect closure when compared to all other treatment groups (p<0.01). In addition, the PEG treated group was able to close the defect us-ing a 10 fold lower concentration of rhBMP-2, as seen by 0.2mg rhBMP-2 + PEG closing at a similar rate to defects

treated with 2.0mg rhBMP-2 + ACS (p<0.05). Histologic examination of the decalcified skulls showed excellent bone formation and near total replacement of the PEG with native tissue at 6 weeks.

Conclusion: In a side by side comparison, the PEG hydrogel delivery system demonstrated the ability to close critical sized cranial defects faster and at a 10 fold lower concentration of rhBMP-2 than what was required using the current ACS delivery system.

Synthetic Hydrogel Scaffold is an Effective Vehicle for Delivery of rhBMP-2 (INFUSE®) to Critical-Sized Calvarial Bone Defects in Rats

Department of Otolaryngology Resident Research Day June 8, 2012 Justin M. Wudel MD 16

Justin M. Wudel MD1, Peter D. Mariner PhD4, David E. Miller2, Erin Genova3, Sven-Olrik Streubel MD1, Kristi S. Anseth PhD4,5

University of Colorado School of Medicine, 1Department of Otolaryngology, 2Department of Radiology, 3Department of Pathology 4University of Colorado Boulder, Department of Chemical and Biological Engineering, 5Howard Hughes Medical Institute *INFUSE® is a registered trademark of Medtronic, Inc.

Just

in M

. Wud

el M

D

Basic Science

Introduction: An alternate route of cochlear stimula-tion via the round window (RW) using active middle ear implants (AMEIs) has been shown to produce physiological responses similar to normal acoustic stimulation including in a model of sta-pes fixation. Pathological conditions such as advanced otosclerosis can preclude delivery of sound energy to the cochlea through the oval window (OW) and/or the RW. Mechanical stimulation through a cochlear third window in the scala tympani (ST) in a chinchilla model (Chinchilla Lanigera) with normal and oc-cluded windows may generate cochlear responses equivalent to acoustic stimuli.

Methods: Cochlear microphonic (CM) and laser Doppler vibrometer (LDV) measurements of stapes and RW velocities were per-formed in 6 ears of 4 chinchillas. Base-line measurements to acoustic sinusoidal stimuli (0.25 to 8 kHz) were made. The measurements were repeated with an AMEI driving either the RW or a third win-dow at the ST before and after sequential oval and round window fixation. Results: AMEI stimulation of the third window pro-duced CM waveforms with morphologies similar to acoustic stimuli. CM thresholds with RW and third window stimulation were frequency-dependent but ranged from 0.25 to 10 and 0.5 to 40 mV,

respectively. Stapes fixation, confirmed by LDV measurements, resulted in a significant frequency dependent impair-ment in CM thresholds up to 13 dB (at < 3 kHz) for RW stimulation and a non-significant frequency dependent decrease in thresholds of up to 10 dB (at > 3 kHz) via third window stimula-tion. OW and RW fixation, confirmed by LDV measurements, resulted in a non-significant mild frequency dependent decrease in CM thresholds from to 1 to 15 dB. Conclusion: Mechanical stimulation with an AMEI through a surgically created cochlear third window into the ST produces CM responses that are nearly identical to those via traditional acoustical stimulation although with decreased efficiency.

Support: AAO-HNSF CORE Resident Research Grant, Otologics Education Grant

Active Middle Ear Implant Third Window Vibroplasty: Electroc-ohleographic Assessment of Direct Mechanical Cochlear Stimula-tion in Experimentally-Induced Oval and Round Window Fixation

Department of Otolaryngology Resident Research Day June 8, 2012 J. Eric Lupo MD, MS 17

J. Eric Lupo MD, MS1, Kanthaiah Koka PhD2, Herman A. Jenkins MD1, Daniel J. Tollin PhD1,2

University of Colorado School of Medicine, 1Department of Otolaryngology 2Department of Physiology and Biophysics

J. Er

ic L

upo

MD,

MS

Basic Science

Objective: The nasal epithelium in rodents contains a population of solitary chemosensory cells (SCCs) innervated by the trigeminal nerve. SCCs detect irritants including bacterially produced molecules, and evoke trigeminally-mediated protective airway reflexes including neurogenic inflammation and changes in respiration. This study will establish the location and impact of SCCs in humans.

Methods: Using RT-PCR and immunohistochem-istry on biopsies from human sinonasal mucosa, we screened biopsies for the presence of taste signaling effectors, which SCCs use to detect irritants. Our experimental group consisted of patients that have pain associated with chronic rhinosinusitis, while the control group consisted of healthy patients undergoing nasal surgery.

Results: RT-PCR revealed expression of the downstream taste signaling effectors al-pha-gustducin, PLC beta2, and TrpM5 in the human septum, the inferior and mid-dle turbinates, and the uncinate process of experimental patients. Immunohisto-chemistry was used to reveal anatomical features of these cells and innervation of the epithelium by peptidergic fibers (polymodal nociceptors) of the trigemi-nal nerve. Taste signaling effectors were expressed in all regions examined in

experimental patients, suggesting the presence of SCCs. However, decreased numbers of the control patients showed expression in the uncinate process, sug-gesting this region may differ between control and experimental patients.

Conclusion: Solitary chemosensory cells are pres-ent in human nasal mucosa. These cells may have an impact on chronic diseases such as chronic rhinosinusitis and al-lergic rhinitis. Further study will better classify how these cells relate to patient characteristics of pain and inflammation.

Support: American Academy of Otolaryngology, ARS Resident Research Grant NIH grants R01DC009820 and P30DC004657Dept. of Otolaryngology Resident Research Funds

Investigation into Nasal Solitary Chemoreceptor Cells

Department of Otolaryngology Resident Research Day June 8, 2012 Henry P. Barham MD 18

Barham HB MD1,3, Tizzano M PhD2,3, Anderson CB MS1,3, Ramakrishnan VR MD1,3 , Kingdom TT MD1,3, Finger TE PhD2,3, Kinnamon SC PhD1,3 University of Colorado School of Medicine, 1Department of Otolaryngology2 Department of Cell and Developmental Biology 3Rocky Mountain Taste and Smell Center

Henr

y P.

Barh

am M

D

Basic Science

Objective: Solitary chemosensory cells (SCCs) are a population of cells that have been found to play a role in trigeminally-mediated protective airway reflexes in rodents. Rodent SCCs express the components of the bitter taste transduc-tion pathway in taste receptor cells of the tongue including T2R bitter taste receptors. Some T2R receptors, such as T2R14 and T2R46, have been shown to respond to a class of chemicals called lactones, which can play a role as quo-rum-sensing molecules for certain bac-teria. Our study aims to identify SCCs in a human population and investigate their possible role in sinonasal disease pro-cesses in humans.

Methods: Biopsies were taken from the sinonasal mucosa of an experimental group of pa-tients undergoing endoscopic sinus sur-gery for chronic rhinosinusitis and a con-trol group of otherwise healthy patients having nasal surgery. These samples were examined with RT-PCR and immu-nohistochemisty to look for the presence of specific genes and gene products associated with the taste transduction pathway associated with SCCs. We also looked at patient Sinonasal Outcome Test (SNOT) and Visual Analog Score (VAS) for pain to see if there was any correlation with receptor expression.

Results: RT-PCR revealed expression of the downstream taste signaling effectors T2R4, T2R14 and T2R46 in the human septum, the inferior and middle turbi-nates, and the uncinate process of both experimental and control patients. Immu-nohistochemistry appears to confirm the presence of SCCs. There was a signifi-cant difference between the SNOT and VAS scores of control and experimental patients, but the relationship to receptor expression needs additional research.

Conclusion: Genes associated with the presence of SCCs have been found in biopsies of human sinonasal tissue, including 2 receptors, T2R14 and T2R46, activated by lactones, which are produced by certain bacteria as quorum-sensing signals. Further study is needed to elucidate their possible significance for chronic rhinosinusitis.

Support: NIH grants R01DC009820 and P30DC004657 Dept. of Otolaryngology Resident Research Funds

Expression of Taste Receptors in Human Sinonasal Mucosa

Department of Otolaryngology Resident Research Day June 8, 2012 Sarah E. Cooper MD 19

Cooper SE MD1,3, Tizzano M PhD2,3, Anderson CB MS1,3, Ramakrishnan VR MD1,3, Kingdom TT MD1,3, Finger TE PhD2,3, Kinnamon SC PhD1,3

University of Colorado School of Medicine, 1Department of Otolaryngology2Department of Cell and Developmental Biology3Rocky Mountain Taste and Smell Center

Sara

h E.

Coo

per M

D

Basic Science

Introduction/Methods: Significant ototoxicity limits use of ami-noglycoside antibiotics (AG). Several mechanisms may contribute to death of both auditory and vestibular hair cells. AG enter outer hair cells of the cochlea through apical transduction channels (Marcotti et al. 2005) and inhibit the basolateral KCNQ4-mediated current (IK,n) by PIP2 sequestration (Leitner et al. 2011). At birth, vestibular hair cells have delayed rectifier K+ current and by the 3rd postnatal week type I hair cells also express a low-voltage activated K+ current that resembles IK,n in outer hair cells. Using whole cell patch clamp, we tested the effects of AG and KCNQ channel modulators on K+ currents (IK) in type I vestibular hair cells isolated from gerbil semicircular canals.

Results: Extracellular neomycin (1 mM) rapidly reduced peak outward IK by 16 ± 4% (n = 9) in mature type I hair cells. Gentami-cin (5 mM) reduced peak IK by 16 ± 3% (n = 8). Intracellular neomycin (1 mM in the patch electrode solution) reduced IK by 17 ± 6% (n = 12). KCNQ modula-tors were used to probe KCNQ channel involvement. XE991 (20 μM) did not reduce IK in mature type I cells and the neomycin-induced reduction in IK was not reversed by the KCNQ agonist flupir-tine (10 μM). Application of intracellular poly-D-lysine (200μg/ml) to sequester

PIP2 did not reduce IK. Extracellular 4-aminopyridine (4-AP, 1mM) blocked a component of IK. Application of extracel-lular AG in the presence of 4-AP gave no further inhibition of IK. In immature type I cells (postnatal days 5-8), extracellular neomycin reduced IK by 19 ± 3% (n = 5). Fluorescent imaging confirmed that externally applied Texas Red conjugated gentamicin was rapidly taken up by ves-tibular hair cells.

Conclusion: AG significantly reduce the 4-AP-sensi-tive IK in early postnatal and mature type I cells. K+ current inhibition differs from that seen in outer hair cells, since it does not involve PIP2 sequestration or KCNQ channels.

Support: American Otological Society and NIDCD DC008297 to KJR

Inhibition of K+ Currents in Type I Vestibular Hair Cells by Aminoglycosides

Department of Otolaryngology Resident Research Day June 8, 2012 Scott E. Mann MD 20

Scott Mann MD1, Hayley L. Ross MD1, Matthew S. Johnson MD1, Frances Meredith MS3, Katherine J. Rennie PhD1,2,3

University of Colorado School of Medicine, 1Department of Otolaryngology2Department of Physiology and Biophysics, 3Neuroscience Program

Scot

t E. M

ann

MD

Basic Science

Objective/Introduction: The “Unified Airway” has been a topic of recent interest, with evidence of common pathologic findings and shared inflammatory pathways between diseases of the sinuses and lower airways. In addition, several studies have shown similarities in microbiology between the upper and lower airways. Bronchiectasis is an uncommon disorder of the lower airways that, at least for immunocompetent adults, is thought to arise from an infectious origin and subsequent neutrophilic protease activity. Nontuberculous mycobacteria (NTM) have been implicated in a subset of these patients, as has chronic inflammation of the paranasal sinuses. NTM are rarely identified in patients with chronic sinusitis, however, they are suspected to be a source of refractory disease in some patients. We hypothesized that chronic NTM pulmonary patients with chronic sinus infection will demonstrate NTM in their sinus cultures. The objective of this study was to examine the prevalence of positive sinus cultures in patients with chronic sinusitis and NTM bronchiectasis.

Methods: IRB approval was obtained for a retrospective chart review at a tertiary care respiratory hospital. The clinical database was searched using ICD-9 codes for patients treated

with diagnoses of bronchiectasis, mycobacterial infection, and sinusitis from 2008 to 2012. Patients who met the specified criteria with available sinus and pulmonary culture results were included in the study. McNemar’s test was used to statistically correlate the presence of positive sinus and pulmonary cultures.

Results:Fifty-five immunocompetent adults were identified with both bronchiectasis and chronic sinusitis. Eleven of these patients with positive pulmonary NTM cultures had endoscopically derived sinus cultures available for review (age 26-73, mean 58; 8/11 female). In these 11 patients no positive sinonasal cultures for AFB were found (p=0.0026).

Conclusion/Discussion:Although the “Unified Airway” theory has been supported by a great deal of research, we found no correlation between sinonasal and pulmonary AFB cultures in patients with known mycobacterial pulmonary disease. This does not mean that NTM are not a potential source of chronic sinus inflammation in this patient population, however. But it can be surmised from this study that performing routine sinus cultures to evaluate for the presence of mycobacterium in these patients may not be necessary and does not appear to change the management strategy.

Correlation of Upper and Lower Airway Acid-Fast Bacilli Cultures in Immunocompetent Adults with Nontuberculous Mycobacterial Bronchiectasis

Department of Otolaryngology Resident Research Day June 8, 2012 Geoffrey R. Ferril MD 21

Geoffrey Ferril MD, Vijay Ramakrishnan MD

University of Colorado School of Medicine, Department of Otolaryngology

Geof

frey

R. F

erril

MD

Clinical

Introduction: Pyoderma gangrenosum (PG) is a skin condition characterized by necrotic ulcers and most commonly occurs on the legs in association with inflammatory bowel disease and rheumatoid arthritis; however, PG rarely involves the head and neck. PG of the head and neck occurs rarely with an estimated incidence of 5% of all PG cases, and very rarely causes nasal septal perforation. At this time, only three cases of nasal septal involvement have been reported in the literature.

Methods:Case report and literature review.

Results:In this report, we present a case of PG-associated nasal septal perforation. A 71 year old male with a history of PG presented with a year history of headache and rhinorrhea. Despite optimal medical treatment for chronic sinusitis, he experienced persistent symptoms. CT scan revealed a widened maxillary antrum and near-total loss of the nasal septum without a prior history of sinusitis, sinus surgery, trauma, and cocaine use. A nasal mucosal biopsy showed chronic inflammatory changes but lacked evidence of necrotizing vasculitis, granulomatous disease, infection, or neoplasm. Additional studies including serologies failed to reveal other systemic inflammatory causes. As a diagnosis of exclusion, PG was diagnosed as

the cause of septal perforation. The differential diagnosis and diagnostic consideration for nasal septal perforations are reviewed.

Conclusion:The patient’s lack of prior sinonasal surgery, trauma or cocaine use combined with serologic testing, histology, and immunohistochemical analysis eliminate other possible causes of nasal septal perforation and point to PG as the most likely cause.

Nasal Septal Perforation Associated with Pyoderma Gangrenosum

Department of Otolaryngology Resident Research Day June 8, 2012 Brook K. McConnell MD 22

Brook K. McConnell MD1, Carrie Marshall MD2, Vijay Ramakrishnan MD1

University of Colorado School of Medicine, 1Department of Otolaryngology2Department of Pathology

Broo

k K.

McC

onne

ll M

D

Clinical

Oropharyngeal Tularemia Presenting with Pharyngitis and a Neck Mass

Department of Otolaryngology Resident Research Day June 8, 2012 Katherine K. Green MD, MS 23

Katherine K. Green MD, MS1, Jeremy Prager MD2

1University of Colorado School of Medicine, Department of Otolaryngology 2Children’s Hospital Colorado, Aurora, CO

Kath

erin

e K.

Gre

en M

D, M

S

Introduction:Francisella (F.) Tularensis is the causative organism of tularemia, a highly infectious facultative gram negative intracellular bacteria. It is considered a potential bio-terrorism agent because of its high viru-lence, transmissibility when aerosolized, and ability to cause severe illness with a very small amount of bacteria. It is a very rare infection in the United States, with an average of 162 cases per year reported between 2000-2008.1 The oropharyngeal form most commonly presents as an exudative tonsillitis with necrotic lymph-adenopathy, but here we present a case which appeared most consistent with an infected branchial cleft anomaly.

Methods:Case report and review of literature.

Results:We present the case of a 7 year-old female with two weeks of sore throat, fa-tigue, intermittent fevers to 39.4°C, and four days of painful left neck swelling. Social history was notable for a camping trip 1 week prior to the onset of symp-toms. A computed tomography (CT) with contrast of the neck was performed, re-vealing a round, hypodense level II mass, appearance consistent with an infected second branchial cleft cyst versus a sup-purative jugulodigastric lymph node. The patient was placed on IV antibiotics, but continued to be symptomatic with fevers and pain. She was taken to the operating room on hospital day two for incision and

drainage of the infected mass, and a thin brown fluid was drained with a penrose left in place. The patient was afebrile and her neck pain improved following surgery. The patient was discharged on amoxicillin-clavulanate, after 72 hours of IV ampicillin-sulbactam, and cultures were negative at the time of discharge.

Several days after discharge the final cul-ture results came back positive for F. tu-larensis. The patient was contacted and her antibiotics were changed to ciproflox-acin. According to the patient’s mother, the left neck had begun to swell again with local erythema and tenderness while on the amoxicillin-clavulanate, but these symptoms resolved after starting the ciprofloxacin. The literature is reviewed and proper identification and treatment is discussed.

Conclusion:Oropharyngeal tularemia is an uncommon presentation of a rare disease. Here we present a patient with tularemia who demonstrated findings consistent with an infected branchial cleft anomaly. The ability of this disease to masquerade as uncomplicated pharyngitis or mononucleosis emphasizes the need for a high index of suspicion in order to make the proper diagnosis, particularly in patients who do not respond to typical first-line treatments for these more common diseases. Proper treatment with specific antimicrobial therapy for F. Tularensis is key.

Clinical

Valérie Côté MD, CM, FRCS(C)

Education/Honors & Awards:Residency:McGill University, Otolaryngology — 2006 - 2011

Medical School:McGill University — Graduated 2006

Abstracts/Presentations: L’otorrhée suite à l’insertion de tubes de ventilation: peut-on la prévenir? Côté V, Dan-iel SJ Presented by Valérie Côté at the 50th Congrès annuel de l’Association ORL du Québec Montreal, Quebec

La rhinoplastie rapide Fanous N, Côté V Presented by Dr. Nabil Fanous at the 50th Congrès annuel de l’Association ORL du Québec Montreal, Quebec

Laryngopharyngeal Reflux and Otitis Media with Effusion Al-Saab F, Manoukian JJ, Al-Sabah, Almot S, Côté V, Nguyen L Presented by Valérie Côté at the International Fed-eration of Oto-Rhino-Laryngological Societies XIX World Congress Sao Paulo, Brazil

Post-thyroidectomy Prediction and Management of Hypocalcemia Côté V, Hier MP, Tamilia M, Young J, Sands N, Black MJ, Payne RJ Presented by Valérie Côté at the In-ternational Federation of Oto-Rhino-Laryngological Societies XIX World Congress Sao Paulo, Brazil

L’otorrhée suite à l’insertion de tubes de ventilation: peut-on la prévenir? Côté V, Daniel SJ Presented by Valérie Côté at the Journées annuelles de formation de l’Association des Médecins Microbiologistes Infectiologues du Québec Bromont, Quebec

Advanced Airway Management Teaching in Otolaryngology Residency: A Survey of Residents Across Canada Côté V, Kus L, Richardson K, Nguyen LHP Presented by Valérie Côté at the Canadian Society of Otolaryngology – Head & Neck Surgery Annual Meeting Halifax, Nova Scotia

Hashimoto’s Thyroiditis as a Risk Factor for Post-Thyroidectomy Hypocalcemia Neaga O, Sands N, Côté V, Tamilia M, Hier MP, Black MJ, Payne RJ Presented at the Cana-dian Society of Otolaryngology – Head & Neck Surgery Annual Meeting Halifax, Nova Scotia

Resident Research Day 2012 – Bio

Department of Otolaryngology Resident Research Day June 8, 2012 Valérie Côté MD, CM, FRCS(C) - BIO 24


fellow bio

Valérie Côté MD, CM, FRCS(C) (cont)

Abstracts/Presentations: Female Gender: A Post-Thyroidectomy Hypocalcemia Risk Factor Sands N, Côté V, Tamilia M, Hier MP, Black MJ, Payne RJ Presented at the American Academy of Oto-laryngology-Head and Neck Surgery Foundation 2008 Annual Meeting & OTO EXPO, September 2008, Chicago

Qualitative Impact of an Introductory Course on « The Art of Surgery » on Otolaryngol-ogy Residency Brousseau V, Fanous N, Côté V, Brousseau E, Yammine N, Shaw K Presented by Valérie Côté at the Canadian Society of Otolaryngology – Head & Neck Surgery Annual Meeting Jasper, Alberta

The « Bikini Lip Reduction » - A Detailed Approach to Hypertrophic Lips Brousseau V, Fanous N, Côté V, Yoskovitch A Presented by Valérie Côté at the Canadian Society of Otolaryngology – Head & Neck Surgery Annual Meeting Jasper, Alberta

Post Thyroidectomy Pain Kalmovich L, Côté V, Payne RJ, Black MJ, Hier MP Presented at the Canadian Society of Otolaryngology – Head & Neck Surgery Annual Meeting Jasper, Alberta

Fine-Needle Aspiration Biopsy of the Thyroid: A Review of Cytopathological Features Predictive of Malignancy Mijovic T, Côté V, Gologan O, Rochon L, Hier MP, Black MJ Presented at the Canadian Society of Otolaryngology – Head & Neck Surgery Annual Meeting Jasper, Alberta

Cost Savings Associated with Post-Thyroidectomy PTH Levels Côté V, Sands N, Hier MP, Black MJ, Tamilia M, MacNamara E, Zhang X, Payne RJ Presented at the Ameri-can Academy of Otolaryngology-Head and Neck Surgery Foundation 2007 Annual Meeting & OTO EXPO, September 16 2007, Washington DC

fT4:Tg Ratio as a Predictor of Thyroid Cancer Recurrence Aron M, Côté V, Tamilia M, Hier MP, Black MJ, Zhang X, Payne RJ Presented at the American Academy of Oto-laryngology-Head and Neck Surgery Foundation 2007 Annual Meeting & OTO EXPO, September 17 2007, Washington DC




fellow bio


Abstracts/Presentations: IFMSA Campaign on Malaria (ICOM) Second International Conference Welcome and Introduction History & evolution of Malaria – Focus on the American situation Econom-ics of Malaria in Endemic Countries IFMSA Campaign on Malaria (ICOM) - Overview Côté V Santiago de Chile, Chile

Pulse Rate and Pulse Rate Variability Decrease After Adenotonsillectomy for Severe Obstructive Sleep Apnea Syndrome Constantin E, McGregor C, Côté V, Brouillette RT Abstract submitted for presentation at the 24th Annual Conference on Sleep Disorders in Infancy & Childhood Rancho Mirage, California, USA

Publications:Endoscopic Excision of an Intraosseous Forehead Dermoid in a Child Emil S, Côté V, Fanous N, J Laparoendosc Adv Surg Tech B. 2011.

The New Genetico-Racial Skin Classification: How to Maximize the Safety of Any Peel or Laser Treatment on Any Asian, Caucasian or Black Patient Fanous N, Côté V, Fa-nous A, Can J Plast Surg. 2011;19(1):9-16

Female Gender as a Risk Factor for Transient Post-Thyroidectomy Hypocalcemia Sands NB, Payne RJ, Côté V, Hier MP, Black MJ, Tamilia M Otolaryngol Head Neck Surg. 2011;145(4):561-4

“Universal Retraction Suture” for the Overprojecting Nasal Tip Fanous N, Fanous A, Al-Sebeih K, Côté V, Can J Plast Surg. 2010;18(3):99-106

The New “Genetico-Racial” Skin Classification: Maximizing the Safety of Skin Treatments for Asians Côté V, Fanous N Facial Plast Surg Clin North Am. 2010 Feb;18(1):99-104

Thyroidectomy: exactly how painful is it? Kalmovich LM, Côté V, Sands N, Black M, Payne R, Hier M J Otolaryngol Head Neck Surg. 2010 Jun;39(3):277-83

Ablative free thyroxine to thyroglobulin ratio as a predictor of differentiated thyroid cancer recurrence Aron M, Côté V, Tamilia M, Hier MP, Black MJ, Zhang X, Payne RJ J Otolaryngol Head Neck Surg. 2009 Oct;38(5):552-8




fellow bio


Publications:Pulse Rate and Pulse Rate Variability Decrease After Adenotonsillectomy for Severe Obstructive Sleep Apnea Syndrome Constantin E, McGregor C, Côté V, Brouillette RT Pediatr Pulmonol. 2008 May;43(5):498-504

Cost Savings Associated with Post-Thyroidectomy PTH Levels Côté V, Sands N, Hier MP, Black MJ, Tamilia M, MacNamara E, Zhang X, Payne RJ Otolaryngol Head Neck Surg. 2008 Feb;138(2):204-8

Sentinel Lymph Node Biopsy in Squamous Cell Carcinoma of the Head and Neck: Where we Stand Now, and Where we Are Going Côté V, Payne RJ, Kost KM, Hier MP J Otolaryngol. 2007 Dec;36(6):344-9

Cancer of the Larynx Côté V, Zeitouni A, Blouin E Patient Information Booklet, Royal Victoria Hospital McGill University Health Center Department of Otolaryngology, Mon-treal, QC

Endoscopic Percutaneous Dilatational Tracheotomy : A Prospective Evaluation of 500 Consecutive Cases. Kost KM. The Laryngoscope. 2005;115S:1-30. Côté V Article and Subject Review Presented at the Royal Victoria Hospital Intensive Care Unit Teaching Rounds Montreal, Quebec




fellow bio

Pamela A. Mudd MD

Education/Honors & Awards:Fellowship: Pediatric Otolaryngology Fellowship — Starting July, 2012Children’s Hospital of Philadelphia

Residency: Administrative Chief Resident — 2011 - 2012

Medical School:Creighton University School of Medicine — Graduated 2007

Undergraduate:B.S. Colorado State University — Graduated 2003

Abstracts/Presentations: P Mudd, J Perkins, J Harwood, S Valdez, G Allen. Early intervention: distraction osteogenesis of the mandible for severe airway obstruction. Podium Presentation at AAO-HNS Meeting Sept 2011 San Francisco, CA

P.Mudd MD; Todd Kingdom MD, Rohit Katial MD; Variations in expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in nasal mucosa of aspirin sensitive versus aspirin tolerant patients with nasal polyposis.Oral presentation at American Rhinologic Society 2010 Annual Meeting,Las Vegas, NV

D Boatright, M Johnson, P Mudd, J Song. Sinonasal undifferentiated carcinoma metastatic to mandible. Poster presentation at American Rhinologic Society 2009 Annual Meeting

P.Mudd MD; K. Lillehei MD, BK. DeMasters MD; T. Kingdom MD. Ectopic pituitary adenoma with apoplexy isolated to the clivus Oct 2009. Poster presentation at American Rhinologic Society 2009 Annual Meeting


Department of Otolaryngology Resident Research Day June 8, 2012 Pamela A. Mudd MD - BIO 28


resident bio

Pamela A. Mudd MD (cont)

Publications:P Mudd, S Hohensee, K Lillehei, T Kingdom, BK Kleinschmidt-DeMasters. Ectopic pituitary adenoma of the clivus presenting with apoplexy: case report and review of the literature. Journal of Neuro-Oncology. Submitted April 2011.

P Mudd, J Perkins, J Harwood, S Valdez, G Allen. Early intervention: distraction osteogenesis of the mandible for severe airway obstruction. Otolaryngology--head and neck surgery, 2012 March, 146(3), 467-472

P Mudd, S Hohensee, K Lillehei, T Kingdom, BK Kleinschmidt-DeMasters. Ectopic pituitary adenoma of the clivus presenting with apoplexy: case report and review of the literature. Clinical Neuropathology, 2012;31(1):24-30).

P Mudd MD, R Katial MD, R Alam MD. S. Hohensee MD, V. Ramakrishnan, T Kingdom. Variations in expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in nasal mucosa of aspirin sensitive versus aspirin tolerant patients with nasal polyposis. Ann Allergy Asthma Immunol. 2011 Oct;107(4):353-9. Epub 2011 Aug 27.

V Scapa, V Ramakrishnan, P Mudd, T Kingdom. Upregulation of RANTES in Nasal Pol-yps from Patients with Cystic Fibrosis. Int Forum Allergy Rhinol, 2011 Jun; 1(3):157-60

S Cass MD, P Mudd MD. Bone Anchored Hearing Osseointegrated Device. Operative Techniques in Otolaryngology-Head and Neck Surgery. Volume 21, Issue 3, September 2010, Pages 197-206 Open Sinus Procedures (Part II) / Techniques for the Ear (Part I)

S Cass MD, P Mudd MD. Pediatric Temporal Bone Tumors. In: C Bluestone, S. Stool, editors. Pediatric Otolaryngology. 5th ed. Published May 2012.

P Mudd MD; S Mann MD, A. Edmunds, MD, PharmD; J. Kalkanis, MD; F. Glatz, MD. Inner Ear, Ototoxicity. eMedicine Journal, May 29 2006, Volume 7, Number 5, Updated May 2012




resident bio

Pamela A. Mudd MD (cont)

Publications:P Mudd, S Hohensee, K Lillehei, T Kingdom, BK Kleinschmidt-DeMasters. Ectopic pituitary adenoma of the clivus presenting with apoplexy: case report and review of the literature. Journal of Neuro-Oncology. Submitted April 2011.

P Mudd MD, R Katial MD, R Alam MD. S. Hohensee MD, V. Ramakrishnan, T Kingdom. Variations in expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in nasal mucosa of aspirin sensitive versus aspirin toler-ant patients with nasal polyposis. Annals of Allergy, Asthma, & Immunology. Submitted April 2011.

S. Cass MD, P Mudd MD. Pediatric Temporal Bone Tumors. In: C Bluestone, S. Stool, editors. Pediatric Otolaryngology. 5th ed. Pending publication, 2011.

V Scapa, V Ramakrishnan, P Mudd, T Kingdom. Upregulation of RANTES in Nasal Polyps from Patients with Cystic Fibrosis. Int Forum Allergy Rhinol, 2011, 1(3:157-160)

S.Cass MD, P. Mudd MD. Bone Anchored Hearing Osseointegrated Device. Operative Techniques in Otolaryngology-Head and Neck Surgery. Volume 21, Issue 3, September 2010, Pages 197-206, Open Sinus Procedures (Part II) / Techniques for the Ear (Part I)

P. Mudd MD; A. Edmunds, MD, PharmD; J. Kalkanis, MD; F. Glatz, MD. Inner Ear, Ototoxicity. eMedicine Journal, May 29 2006, Volume 7, Number 5, Updated May 2010




resident bio

Adam M. Holdt MD

Education/Honors & Awards:Residency: Outstanding Clinical Research Award-Resident Research Day — 2010

Medical School:University of Chicago, Pritzker — Graduated 2007

Undergraduate:B.S. Northwestern University — Graduated 2002 Cum Laude

Abstracts/Presentations: Holdt AM, Goddard J. Outpatient bridging of anticoagulation for tonsillectomy. AAO-HNS 2010 Annual Meeting, Boston, MA, September 28, 2010.

Ward MS, Khoobehi A, Lavik EB, Holdt AM, Mwidau AA, Langer RL, Young MJ. Bio-degradable microspheres containing glial derived neurotrophic factor encourage long term survival of retinal ganglion cells in the DBA/2J mouse glaucoma model. Invest Ophthalmol Vis Sci. 2004;45 (Supp 1):U509.

Khoobehi A, Lavik EB, Holdt AM, Mwidau AA, Langer RL, Young, MJ. Biodegradable microspheres with long-term release of survival factors in the DBA/2 J mouse model of glaucoma. Invest Ophthalmol Vis Sci. 2003;44 (Supp 1):U22.

Publications:Nkansah MK, Tzeng SY, Holdt AM and Lavik EB. Poly(lactic-co-glycolic acid) nano-spheres and microspheres for short- and long-term delivery of bioactive ciliary neuro-trophic factor. Biotechnology and Bioengineering. 2008;100:1010-1019.


Department of Otolaryngology Resident Research Day June 8, 2012 Adam M. Holdt MD - BIO 31


resident bio

Jacob S. Minor MD

Education/Honors & Awards:Residency: Outstanding Basic Science Award-Resident Research Day — 2010Highest In-Service Exam Score — 2010

Medical School:Baylor College of Medicine — Graduated 2007International Health TrackPresidential Scholar Award

Undergraduate:B.S. Texas A&M University — Graduated 2002 Summa Cum Laude

Abstracts/Presentations: Minor JS, Eusterman VD. Pneumolabyrinth and Pneumocochlea – Natural History, In-terventions, and Outcomes. Combined Otolaryngology Spring Meeting. 2010.

Publications:Minor J, Reziq M, Wine T. Mummified leiomyoma of the midline anterior neck: Case report and literature review. ENT Journal. (in press)

Minor JS, Epstein JB. The Tongue and Burning Mouth Syndrome. Otolaryngologic Clinics of North America. Feb 2011.

Credille KM, Minor JS, Barnhart KF, Lee E, Cox ML, Tucker KA, Diegel KL, Venta PJ, Hohl D, Huber M, Dunstan RW. Transglutaminase 1-deficient recessive lamellar ich-thyosis associated with a LINE-1 insertion in Jack Russell terrier dogs. British Journal of Dermatology. 2009 Aug;161(2):265-72. Epub 2009 Apr 24.


Department of Otolaryngology Resident Research Day June 8, 2012 Jacob S. Minor MD - BIO 32


resident bio

Jacob S. Minor MD (cont)

Publications:Minor JS, Tang HY, Pereira FA, Alford RL. DNA sequence analysis of SLC26A5, en-coding prestin, in a patient-control cohort: identification of fourteen novel DNA se-quence variations. PLoS ONE. 2009; 4(6):e5762

Minor J, Dunstan R, Guyon R, André C, Barnhart K, Credille K. Comparative sequence analysis and radiation hybrid mapping of the canine keratin 10 gene. DNA Sequence. 2005; 16(2): 89–95.

Credille KM, Barnhart KF, Minor JS, Dunstan RW. Mild recessive epidermolytic hyper-keratosis associated with a novel keratin 10 donor splice-site mutation in a family of Norfolk terrier dogs. British Journal of Dermatology. 2005; 153: 51–58.


Department of Otolaryngology Resident Research Day June 8, 2012 Jacob S. Minor MD - BIO 33


resident bio

J. Eric Lupo MD, MS

Education/Honors & Awards:Residency: Outstanding Resident Research Day - First Place - 2011Association for Research in Otolaryngology Travel Award — 2009 - 2011CORE AAO-HNSF Resident Research Grant — 2009

Medical School:University of Colorado School of Medicine — Graduated 2008UCD Professional Development Award

Graduate:M.S. Stanford University — Graduated 2001

Undergraduate:M.S. Stanford University — Graduated 2001B.S. University of Colorado Boulder — Graduated 1997 Colorado Engineering Council Certificate of Merit

Abstracts/Presentations: Lupo JE, Lupo VK, Uhler K, Zaccarro K, Cass SP. 2012. Cochlear implantation in bilateral sporadic vestibular schwannoma: consideration of hearing and vestibular preservation. 12th International Conference on Cochlear Implants. Abs: 164.

Cochlear implantation in bilateral sporadic vestibular schwannoma: consideration of hearing and vestibular preservation. 12th International Conference on Cochlear Implants. Baltimore, MD 5/2012

Lupo JE, Koka K, Jenkins HA, Tollin DJ. 2012. Optimizing active middle ear implant stimulation of the round window: Implant-membrane coupling factors influencing stimulation efficiency. 12th International Conference of Cochlear Implants Abs: 174.

Lupo JE, Koka K, Jenkins HA, Tollin DJ. 2012. Cochlear stimulation via third window vibroplasty in Chinchilla Lanigera: Effects of round window and oval window occlusion. ARO Abs: 597

Optimizing the Performance of Active Middle Ear Implants: Basic Studies. University of Colorado Department of Otolaryngology MidWinter Meeting. Vail, CO 2/7/12


Department of Otolaryngology Resident Research Day June 8, 2012 J. Eric Lupo MD, MS - BIO 34


resident bio

J. Eric Lupo MD, MS (cont)Abstracts/Presentations:Third window vibroplasty with an active middle ear implant: Assessment of physiologic responses in a model of stapes fixation in Chinchilla Lanigera. American Otological Society. Chicago, IL 4/30/2011

Lupo JE, Koka K, Jenkins HA, Tollin DJ. 2011. Third Window Vibroplasty: Assessment of Physiologic Responses in a Model of Stapes Fixation AOS Abs accepted

Lupo JE, Koka K, Hyde BJ, Jenkins HA, Tollin DJ. 2011. Active Middle Ear Implant Stimulation of the Round Window: Physiologic Assessment of Factors Influencing Implant Coupling to the Round Window Membrane. ARO Abs.: 207.

Hyde BJ, Lupo JE, Koka K, Tollin DJ. 2011. Effect of Unilateral Conductive Hearing Loss on the Amplitudes and Phases of the Cochlear Microphonic during Bone Conduction: an Experimental Investigation of the Classic Weber Test. ARO Abs.: 62.

Thornton JL, Chevallier KM, Koka K, Lupo JE, Tollin DJ. 2011. Experimentally-induced Unilateral Conductive Hearing Loss Substantially Alters the Interaural Level and Time Difference Cues to Sound Location. ARO abs.: 733.

Chevallier KM, Thornton JL, Koka K, Lupo JE, Uhler KM, Tollin DJ. 2011. Conductive Hearing Loss Induced by Experimental Middle Ear Effusion in a Chinchilla Model Reveals Impaired TM-coupled Ossicular Chain Movement. ARO abs.: 61.

Lupo JE, Leuin S, Kelley PE. 2010. Anterior Laryngofissure Approach to an Airway Foreign Body After Migration Into the Paraglottic Space. ABEA Abs #10

Lupo JE, Deveze A, Koka K, Tringali S, Jenkins HA, Tollin DJ. 2010. Techniques to improve the efficiency of active middle ear implants: Effect of different coupling methods to the ossicular chain. Assoc. Res. Otolaryngology. Abs.: 926.

Thornton JL, Koka K, Lupo JE, Jones HG, Tollin DJ. 2010. Validation of a Virtual Conductive Hearing Loss Technique in an Animal Model. Assoc Res Otolaryngology. Abs.: 316.

Active middle ear implant cochlear stimulation in experimental models of stapes fixation. Otosclerosis Study Group 72nd Annual Meeting. Boston. 9/25/10




resident bio

J. Eric Lupo MD, MS (cont)

Abstracts/Presentations:Holland NJ, Koka K, Lupo JE, Tollin DJ, Jenkins HA. 2009. Feasibility study of round window stimulation with the MET-V implantable middle ear device: A temporal bone study. Hear Res 263(1-2): 251

Lupo JE, Koka K, Holland NJ, Jenkins HA, Tollin DJ. 2009, Electrocochleographic Assessment of Mechanical Stimulation of the Round Window, Assoc. Res. Otolaryngol. Abs. 33.

Holland NJ, Koka K, Lupo JE, Jenkins HA, Tollin DJ. 2009, Cochlear responsiveness to frequency-independent constant-velocity direct mechanical stimulation of the round window (RW) with the Otologics MET-V transducer. Assoc. Res. Otolaryngol. Abs. 60.

Prospective Electrophysiologic Findings of Round Window Stimulation in a Model of Experimentally-induced Stapes Fixation. American Otological Society. Pheonix, AZ 5/28/2009

Lupo JE, Koka K, Tollin DJ. 2008, The Effects of Experimentally-induced Conductive Hearing Loss on Spectral and Temporal Aspects of Sound Transmission Through the Ear, Assoc. Res. Otolaryngol. Abs.: 881.

Koka K, Jones HG, Lupo JE, and Tollin DJ. 2008, The acoustical cues to sound location in the adult Chinchilla: Measurements of Directional Transfer Functions (DTFs), Assoc. Res. Otolaryngol. Abs.: 880.

The Effects of experimentally-induced conductive hearing loss on spectral and temporal aspects of sound transmission through the ear. American Federation for Medical Research Western Regional Meeting. Carmel, CA 2/1/2008

Publications:Thornton JL, Chevalier KM, Koka K, Lupo JE, Tollin DJ. 2012. The conductive hearing loss due to an experimentally-induced middle ear effusion alters the interaural level and time difference cues to sound location. JARO. DOI 10.1007/s10162-012-0335-2.




resident bio

J. Eric Lupo MD, MS (cont)

Publications:Lupo JE, Koka K, Jenkins HA, Tollin DJ. 2011. Third window vibroplasty with an active middle ear implant: Assessment of physiologic responses in a model of stapes fixation in Chinchilla Lanigera. Otol Neurotol. 33(3):425-31.

Lupo JE, Koka K, Hyde BJ, Jenkins HA, Tollin DJ. 2011. Physiologic Assessment of Active Middle Ear Implant Coupling to the Round Window in Chinchilla Lanigera. Otolaryngol Head and Neck Surg. 145(4):641-7

Lupo JE, Leuin S, Kelley PE. 2011. Anterior Laryngofissure Approach to an Airway Foreign Body After Migration Into the Paraglottic Space. Laryngoscope. 121(10):2159-61

Lupo JE, Koka K, Thornton JL, Tollin DJ. 2010. The Effects of Experimentally-induced Conductive Hearing Loss on Spectral and Temporal Aspects of Sound Transmission Through the Ear. Hear Res. 272(1-2):30-41

Koka K, Jones HG, Thornton JL, Lupo JE, Tollin DJ. 2010. Sound pressure transformations by the head and pinnae of the adult Chinchilla (Chinchilla lanigera). Hear Res. 272(1-2):135-47

Koka K, Holland NJ, Lupo JE, Jenkins HA, Tollin DJ. 2010. Electrocochleographic and Mechanical Assessment of Round Window Stimulation with an Active Middle Ear Prosthesis. Hear Res. 263(1-2):128-3

Lupo JE, Koka K, Holland NJ, Jenkins HA, Tollin DJ. 2009. Prospective Electrophysiologic Findings of Round Window Stimulation in a Model of Experimentally-induced Stapes Fixation. Otol Neurotol Dec 30(8):1215-24.




resident bio

Justin M. Wudel MD

Education/Honors & Awards:Residency: Outstanding Resident Research Day - Second Place - 2011

Medical School: University of Minnesota — Graduated 2008 Alpha Omega Alpha

Undergraduate:B.S. University of Minnesota — Graduated 2004

Abstracts/Presentations:Wudel J, Valdez S., Perkins K., Blanchard A., Allen G. Efficacy of recombinant hu-man bone morphogenic protein (rhBMP-2) in the treatment of maxillary alveolar clefts. American Academy of Otolaryngology- Head and Neck Surgery, September 2012- Washington, DC

Wudel J, Wine T. Immune reconstitution causing regression of Kaposi’s sarcome. American Academy of Otolaryngology- Head and Neck Surgery, September 2011- San Francisco, CA

Wudel J, Kingdom T. Seronegative sinonasal Wegener’s granulomatosis: A clinical challenge. 2010 September - Boston, MA

Wudel J, Kingdom T, Durairaj V. Outcomes after dacryocystorhinostomy in patients with nasolacrimal duct obstruction secondary to sarcoidosis. American Rhinologic So-ciety, April 2010 - Las Vegas, NV

Wudel J, Sidman J, Landers T, Rimell F. Efficacy of balloon sinuplasty in the pediatric population. American Society of Pediatric Otolaryngology, May 2008 – Orlando, FL

Wudel J, Wuertz B, Ondrey F. Inhibition of Adenoid Cystic Cancer via Combination Chemotherapy and PPAR Gamma Activation in Vitro. Triological Society Middle Section Meeting, January 2008 – Chicago, IL


Department of Otolaryngology Resident Research Day June 8, 2012 Justin M. Wudel MD - BIO 38


resident bio

Justin M. Wudel MD (cont)

Publications:Green K, Wudel J, Prager J. Oropharyngeal tularemia in a child presenting with pharyngitis and a neck mass. 2012 March (Submitted)

Barham H, Wudel J, Enzenauer H, Chan K. Congenital nasolacrimal duct cyst: An argument for a genetic basis. Journal of Allergy and Rhinology. 2011 October (Accepted for publication).

Wudel J, Valdez S, Perkins J, Blanchard A, Allen G. Efficacy of recombinant human bone morphogenic protein (rhBMP-2) in the treatment of maxillary alveolar clefts. 2011 October (In Process)

Wudel J, Mariner P, Brown N, Streubel S, Anseth K. Synthetic hydrogel scaffold is an effective vehicle for the delivery of rhBMP-2 (InFUSE) to critical sized calvarial defects in rats. 2011 October (Submitted to Journal of Orthopedic Research)

Wudel J, Brown N, Mariner, P, Anseth K, Streubel S. In vivo application of polyethelene glycol hydrogel to promote wound healing in full thickness skin defects. 2011 September (Submitted to Journal of Tissue Engineering and Regenerative Medicine)

Wudel J, Wine T. Immune reconstitution causing regression of Kaposi’s sarcoma. Ear Nose Throat J. 2011 September (Accepted for publication)

Wudel J, Kingdom T. Seronegative sinonasal Wegener’s granulomatosis: A clinical challenge. 2010 September (In process)

Wudel J, Kingdom T, Durairaj V. Outcomes after dacryocystorhinostomy in patients with nasolacrimal duct obstruction secondary to sarcoidosis. 2010 April (In process)

Wudel J, Winkler A. Temporal Artery Biopsy. emedicine.com. Jan 2010. http://emedi-cine.medscape.com/article/1520091-overview.

Gonda K, Wudel J, Nelson D, Katoku-Kikyo N, Reed P, Tamada H, Kikyo N. Requirement of the protein B23 for nucleolar disassembly induced by the FRGY2a family proteins. Journal of Biological Chemistry. 2006 Mar; 281 (12): 8153-8160




resident bio

Justin M. Wudel MD (cont)

Publications:Tamada H, Van Thuan N, Reed P, Nelson D, Katoku-Kikyo N, Wudel J, Wakayama T, Kikyo N. Chromatin decondensation and nuclear reprogramming by nucleoplasmin. Molecular and Cellular Biology. 2006 Feb; 26 (4): 1259-1271

Wudel J, Kikyo N. Fluorescence recovery after photobleaching (FRAP) to detect shuttling of the nucleolar protein B23. Bio-Rad Cell Science. 2004 Jun; (1): 1-8

Gonda K, Fowler J, Katoku-Kikyo N, Haroldson J, Wudel J, Kikyo N. Reversible disassembly of somatic nucleoli by the germ cell proteins FRGY2a and FRGY2b. Nature Cell Biology. 2003 Mar; 5(3): 205-210.




resident bio

Henry P. Barham MD

Education/Honors & Awards:Medical School:Louisiana State University HSC — Graduated 2009AMA Foundation Scholars Fund RecipientKey Note Speaker to the Louisiana State University Board of Supervisors

Undergraduate:B.S. Louisiana State University — Graduated 2004 Louisiana State University Board of Supervisors Scholarship, Lifetime

Abstracts/Presentations: HP Barham, M Tizzano, CB Anderson, VR Ramakrishnan, TT Kingdom, TE Finger, SC Kinnamon. Solitary chemosensory cells in biopsies of human nasal mucosa. Associa-tion for Chemoreception Sciences 34th Annual Meeting in Huntington Beach, California, April 26, 2012.

HP Barham, JM Wudel, RW Enzenauer, KH Chan. Congenital Nasolacrimal Duct Cyst: An argument for a genetic basis. Triological Society Combined Sections Meeting in Miami Beach, Florida, January 26-28, 2012.

HP Barham, S Said, VR Ramakrishnan. Sinonasal Tumor with two distinct histologies. The American Rhinologic Society annual meeting in San Fransisco, September 2011.

JH Wood, DA Partrick, HP Barham, SH Travers, DD Bensard, RC McIntyre, Jr. Pediat-ric Thyroidectomy: A collaborative approach. 42ndAnnual Meeting, Canadian Associa-tion of Paediatric Surgeons, Saskatoon, Saskatchewan, Canada, September 24, 2010.

H. P. Barham, T. T. Kingdom. Chondrosarcoma of the Nasal Cavity: A Case Report. Presented at The American Rhinologic Society annual meeting in Boston September 2010


Department of Otolaryngology Resident Research Day June 8, 2012 Henry P. Barham MD - BIO 41


resident bio

Henry P. Barham MD (cont)

Publications:Pediatric Thyroidectomy: a collaborative surgical approach. JH Wood, DA Partrick, HP Barham, DD Bensard, SH Travers, JL Bruny, RC McIntyre, Jr. Journal of Pediatric Sur-gery forthcoming 2011.

Curcumin inhibits HNSCC by modulating the Akt/mTOR pathway, C. A. Clark, Y. Rong, X. Rong, S. Shah, H. Barham, C. O. Nathan Oral Oncology, ISSN: 1368-8375, Vol: 3, Issue: 1, Date: 2009-01-01


Department of Otolaryngology Resident Research Day June 8, 2012 Henry P. Barham MD - BIO 42


resident bio


Department of Otolaryngology Resident Research Day June 8, 2012 Sarah E. Cooper MD - BIO 43


resident bio

Sarah E. Cooper MD

Education/Honors & Awards:Internship:University of California at San Francisco, Department of Surgery — June, 2009 - June, 2010

Medical School:Texas Tech University HSC — Graduated 2009Alpha Omega AlphaDean’s Recognition Award

Undergraduate:B.S. The University of Notre Dame — Graduated 2003

Publications:Hwang JP, Cooper SE, Gagel RF. Mechanisms of Complications of Malignancy. Mechanisms of the Complications of Malignancy. Ed. Yeung et al. Shelton: BC Decker Inc, 2009. 3-9.

Peters, M.B., Jr., Camacho, D., Ojeda, H., Reichenbach, D., Knauer, E.M., Yahanda, A., Cooper, S.E., Sweeney, J.F. 2004 Nov. Laparoscopic splenectomy for immune thrombocytopenic purpura: defining the learning curve. Am J Surg, 188:522-525.

Peters, M.B., Jr., Obermeyer, R.J., Ojeda, H.F., Knauer, E.M., Mille, M.P., Ertan, A., Cooper, S., Sweeney, J.F. 2005 Jul-Sep. Laparoscopic management of colonic lipomas: A case report and review of the literature. JSLS 9(3):324-4.


Department of Otolaryngology Resident Research Day June 8, 2012 Scott E. Mann MD - BIO 44


resident bio

Scott E. Mann MD

Education/Honors & Awards:Residency: Outstanding Resident Research Day - Honorable Mention - 2011

Medical School:University of Colorado SOM — Graduated 2009Alpha Omega AlphaExcellence in Medical Humanities AwardPresbyterian/St. Luke’s Hospital Auxiliary Scholarship

Undergraduate:B.M. University of Denver — Graduated 2003 Magna Cum LaudePhi Beta Kappa

Abstracts/Presentations: Scott Mann, Hayley Ross, Matthew Johnson, Frances Meredith, Katie Rennie. Inhibition of K+ Currents in Type I Vestibular Hair Cells by Aminoglycosides. Association for Research in Otolaryngology 35th Mid-winter meeting, February 2012

Frances Meredith, Scott Mann, Katherine Rennie. Characterization of a Hyperpolarization-Activated Current (Ih) in Vestibular Calyx Terminals. Association for Research in Otolaryngology 35th Mid-winter meeting, February 2012

Mann SE, Dhawan R, Rennie KJ. Signaling Mechanisms at the Type I Hair Cell/Calyx Synapse. Association for Research in Otolaryngology Midwinter Research Meeting, Baltimore April 19, 2009.

Publications:P. Mudd, S Mann, A. Edmunds, J Kalkanis, F Glatz. Inner Ear, Ototoxicity. eMedicine Journal, May 29 2006, Volume 7, Number 5, Updated May 2012

Dhawan R, Mann SE, Meredith FL, Rennie KJ. K+ currents in isolated vestibular afferent calyx terminals. J Assoc Res Otolaryngol. 2010 Sep;11(3):463-76. Epub 2010 Apr 21.

Moore RD, Schuman TA, Scott TA, Mann SE, Davidson MA, Labadie RF. Exostoses of the external auditory canal in white-water kayakers. Laryngoscope. 2010 Mar;120(3):582-90.


Department of Otolaryngology Resident Research Day June 8, 2012 Matthew J. Whinery MD - BIO 45


resident bio

Matthew J. Whinery MD

Education/Honors & Awards:Residency: Outstanding Resident Research Day - Best Case Review - 2011Highest In-Service Exam - 2011

Medical School:UT Southwestern — Graduated 2009Alpha Omega Alpha

Undergraduate:B.S. Texas A&M University — Graduated 2005 Summa Cum LaudePhi Beta KappaPhi Kappa Phi

Abstracts/Presentations: Pierce JR Jr., Pittard AIE, Denison AV, Strickland S, Arellano A, Whinery M, Richardson JM. Retrospective study of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infections in a west Texas community, 2004. 135th Annual Meeting, American Public Health Association, Washington, DC, 11/05/07.

Strickland S, Arellano A, Whinery M, Pittard A, Pierce JR Jr. Community-aquired methicillin-resistant Staphylococcus aureus (CA-MRSA) in Potter and Randall Counties, 2004. 81st Annual Educational Conference, Texas Public Health Association, 04/24/06, Plano, TX.


Department of Otolaryngology Resident Research Day June 8, 2012 Geoffrey R. Ferril MD - BIO 46


resident bio

Geoffrey R. Ferril MD

Education/Honors & Awards:Medical School:The University of Mississippi SOM — Graduated 2010

Undergraduate:B.A. The University of Mississippi — Graduated 2006Magna Cum LaudePhi Beta KappaPhi Kappa Phi

Abstracts/Presentations: Compliance with colorectal cancer surveillance among patients with ulcerative colitis. Hamat H, Reddy G, Thurman D, Kodali U, Ferril G. Inflammatory Bowel Diseases Vol-ume 14 (2008): s14-s15., 2007

Publications:Alexander, Barbara T.; Hendon, Andrew E.; Ferril, Geoffrey; Dwyer, Terry M. Renal de-nervation abolishes hypertension in low-birth-weight offspring from pregnant rats with reduced uterine perfusion. Hypertension Volume 45 (2005): 754-758, 2004


Department of Otolaryngology Resident Research Day June 8, 2012 Katherine K. Green MD, MS - BIO 47


resident bio

Katherine K. Green MD, MS

Education/Honors & Awards:Medical School:University of Southern California Keck SOM — Graduated 2010

Graduate:M.S. Rush University — Graduated 2006

Undergraduate:B.A. Northwestern University — Graduated 2004

Publications:Maceri DR, Kokot N, Green K, Montgomery V, Sharifi J. Split central venous sampling of parathyroid hormone: an adjunct to surgical exploration. Head Neck. 2011 Dec;33(12):1715-8. doi: 10.1002/hed.21659. Epub 2011 Feb 1.

Derebery MJ, Fisher LM, Berliner K, Chung J., &. Green K. Outcomes of Endolymphatic Shunt Surgery for Meniere’s Disease: Comparison With Intratympanic Gentamicin on Vertigo Control and Hearing Loss. Otology & Neurotology. 31: 649-655; 2010

Submitted to International Journal of Pediatric Otolaryngology: Green K, Wudel J, Prager J. Oropharyngeal tularemia in a patient presenting with an infected branchial cleft anomaly.

In submission: The efficacy of treatments for autoimmune inner ear disorder. Derebery, M.J., Green, K., Chung, J., & Fisher, LM.


Department of Otolaryngology Resident Research Day June 8, 2012 Brook K. McConnell MD - BIO 48


resident bio

Brook K. McConnell MD

Education/Honors & Awards:Medical School:University of Colorado SOM — Graduated 2010

Undergraduate:B.A. University of Colorado Boulder — Graduated 2005

Abstracts/Presentations: Poster presentation COSM 2012: Nasal septal perforation associated with pyoderma gangrenosum. 2012 Abstract submission to AMP 55th Annual Meeting, presentation on Genetics in cancer adaptation. 2008


Department of Otolaryngology Resident Research Day June 8, 2012 Leah J. Abrass MD - BIO 49


resident bio

Leah J. Abrass MD

Education/Honors & Awards:Medical School:Northwestern University, Feinberg SOM — Graduated 2011 Alpha Omega Alpha

Undergraduate:B.S. University of Southern California — Graduated 2006Cum Laude

Abstracts/Presentations: Ramakrishnan V, Feazel L, Abrass L, Frank D. “Microbiome and S. Aureus in Chronic Rhinosinusitis.” Oral presentation at Combined Otolaryngology Spring Meetings. San Diego, CA. April 2012.

Abrass L, Chandra R.. (2010, September). Factors associated with CT status in patients presenting with a history of CRS. Oral presentation presented at: American Rhinology Society; Boston, MA.

Publications:Abrass L, Chandra R, Conley D, Tan B, Kern R. “Factors associated with CT status in patients presenting with a history of CRS.” International Forum of Allergy & Rhinology. May 2011; 1(3):178-82.


Department of Otolaryngology Resident Research Day June 8, 2012 Justin T. Casey MD - BIO 50


resident bio

Justin T. Casey MD

Education/Honors & Awards:Medical School:Florida State University SOM — Graduated 2011

Undergraduate:

B.S. Rensselaer Polytechnic Insitute — Graduated 2006

Abstracts/Presentations: Maitland C., Lapointe L., Casey J. (2011, February). Effects of cognitive-linguistic load and dopaminergic medication cycle on gait, balance, and risk for injurious falls in Parkinson’s Disease. Poster presented at: 3rd International Conference on Drug Discovery & Therapy; Dubai, United Arab Emirates.

Casey, JT., Kosko, JR. (2010, December). Retropharyngeal Abscesses: Surgical vs. Medical Management. Poster presented at Society for Ear, Nose, and Throat Advancement in Children Annual Meeting; Cincinnati, OH.

Publications:Casey JT., Maitland CG., Optic disc head metastases presenting without edema or visible mass. Journal of Neuro-Ophthalmology. Pub Status: Submitted.

Maitland CG., Casey JT., Davis TM., Optic neuritis in rapidly progressive AIDS exncephalopathy. Journal of Ophthalmology. Pub status: Accepted.


Department of Otolaryngology Resident Research Day June 8, 2012 Benjamin M. Milam MD - BIO 51


resident bio

Benjamin M. Milam MD

Education/Honors & Awards:Medical School:University of Virginia — Graduated 2011

Undergraduate:B.S. University of Virginia — Graduated 2006 Phi Beta Kappa

Abstracts/Presentations: Shonka Jr DC, Milam BM, Jameson MJ. Suprafascial harvest of the radial forearm free flap decreases the risk of postoperative tendon exposure. Abstract presented at the American Head and Neck Society meeting which is part of the Combined Otolaryngo-logical Spring Meetings in May 2011 in Chicago.

Milam BM, Melstrom LG, Strouch MJ, McGill JJ, Salabat MR, Van Beek DB, Heiferman DM, Heiferman MJ, Ding XZ, Adrian TA, Bentrem DJ. Growth inhibitory effects of gemcitabine are augmented by the flavonoid apigenin in human pancreatic cancer cells. Pancreas. 2007 Nov; 35(4): 417. Abstract.

Publications:Schneyer MS, Milam BM, Payne SC. Sites of attachment of Schneiderian Papilloma. International Forum of Allergy and Rhinology. 2011; 1:324–328.

Strouch MJ, Milam BM, Melstrom LG, McGill JJ, Salabat MR, Ujiki MB, Ding XZ, Bentrem DJ. The flavonoid apigenin potentiates the growth inhibitory effects of gem-citabine and abrogates gemcitabine resistance in human pancreatic cancer cells. Pan-creas. 2009 May;38(4):409-15.

Melstrom LG, Salabat MR, Ding XZ, Milam BM, Strouch M, Pelling JC, Bentrem DJ. Apigenin inhibits the GLUT-1 glucose transporter and the phosphoinositide 3-kinase/Akt pathway in human pancreatic cancer cells. Pancreas. 2008 Nov;37(4):426-31.


Department of Otolaryngology Resident Research Day June 8, 2012 Benjamin M. Milam MD - BIO 52


resident bio

Benjamin M. Milam MD (cont)

Publications: Salabat MR, Melstrom LG, Strouch MJ, Ding XZ, Milam BM, Ujiki MB, Chen C, Pelling JC, Rao S, Grippo PJ, McGarry TJ, Bentrem DJ. Geminin is overexpressed in human pancreatic cancer and downregulated by the bioflavanoid apigenin in pancreatic cancer cell lines. Molecular Carcinogenesis. 2008 Nov;47(11):835-44.

Ujiki MB, Ding XZ, Salabat MR, Bentrem DJ, Golkar L, Milam B, Talamonti MS, Bell RH Jr, Iwamura T, Adrian TE. Apigenin inhibits pancreatic cancer cell proliferation through G2/M cell cycle arrest. Molecular Cancer. 2006 Dec 29; 5:76.

Ujiki MB, Milam B, Ding XZ, Roginsky AB, Salabat MR, Talamonti MS, Bell RH, Gu W, Silverman RB, Adrian TE.. A novel peptide sansalvamide analogue inhibits pancreatic cancer cell growth through G0/G1 cell-cycle arrest. Biochemical & Biophysical Re-search Communications. 2006 Feb 24;340(4):1224-8.

Heckman J, Samie A, Bessong P, Ntsieni M, Hamandi H, Kohler M, Milam B, Scriver J, Dillingham R. Anaemia among clinically well under-fives attending a community health centre in Venda, Limpopo Province. South African Medical Journal. 2010 July;100(7):445-448.

Poster Display SessionViewing from 12:00pm – 12:30pm

1. Cochlear Stimulation Via Third Window Vibroplasty in Chinchilla Lanigera: Effects of Round WIndow and Oval Window Occlusion J. Eric Lupo, MD, MS, Kanthaiah Koka, MD, Herman Jenkins, MD, Daniel J. Tollin, PhD

2. The Dependence of the Binaural Interaction Component (BIC) of the Auditory Brainstem Response (ABR) on the Binaural Cues to Sound Source Location on the Chinchilla Alexander T. Ferber, MD, Jennifer L. Thornton, PhD, Kanthaiah Koka, MD, Daniel J. Tollin, PhD

3. Neural Coding of Interaural Level Differences in the Inferior Colliculus is Altered in Adult Animals as a Result of Temporary Unilateral Conductive Hearing Loss Jennifer L. Thornton, PhD, Kanthaiah Koka, MD, Heath G. Jones, Dalian Ding, Richard Salvi, PhD, Daniel J. Tollin, PhD

4. Optimizing Active Middle Ear Implant Stimulation of the Round Window: Implant-Membrane Coupling Factors Influencing Stimulation Efficiency J. Eric Lupo, MD, MS, Kanthaiah Koka, MD, Herman Jenkins, MD, Daniel J. Tollin, PhD

5. Solitary Chemosensory Cells in Biopsies of Human Nasal Mucosa Barham HB, MD, Tizzano M, MD, Anderson CB, Cooper S, MD, Ramakrishnan VR, MD, Kingdom TT, MD, Finger TE, PhD, Kinnamon SC, PhD

6. Nasal Septal Perforation Associated with Pyoderma Gangrenosum Brook K. McConnell, MD, Carrie B. Marshall, MD, Vijay R. Ramakrishnan, MD

7. Utility of 18FDG-PET in the Initial Diagnosis and Workup of Sinonasal Malignancy Vijay R. Ramakrishnan, MD, Alexander G. Chiu, MD, James N. Palmer, MD, David W. Kennedy, MD, Bert W. O’Malley, MD

8. Sinonasal Malignancy with Two Distinct Histologies Henry P. Barham, MD, Sherif Said, MD, PhD, Vijay R. Ramakrishnan, MD

Resident Research Day 2012 – Poster Presentations

Department of Otolaryngology Resident Research Day June 8, 2012 Posters 53


posters

9. Overexpression of PIK3CA Oncogene Drives Head and Neck Cancer Invasion and Metastasis Fang Zhang, MD, Jacob Minor, MD, Yuexin Li, MD, Stephen Weber, Neil Gross, MD, John Song, MD, Sherif Said, MD, PhD, Xiao-Jing Wang, MD, PhD, Natalie Serkova, PhD, Shi-Long Lu, MD, PhD

10. Immune Reconstitution Causing Regression of Kaposi’s Sarcoma Justin M. Wudel, MD, Todd Wine, MD

11. Congenital Nasolacrimal Duct Cyst/Dacryocystocele: An Argument for a Genetic Basis Henry P. Barham, MD, Justin M. Wudel, MD, Robert W. Enzenauer, MD, MPH, Kenny H. Chan, MD

Resident Research Day 2012 – Poster Presentations

Department of Otolaryngology Resident Research Day June 8, 2012 Posters 54


posters

department of otolaryngology - denver, colorado · welcome to the department of otolaryngology...

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