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Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012 Herbert Gross 1

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Page 1: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Design and Correction of optical Systems

Part 9: Fourier opticcal image formatioin

Summer term 2012

Herbert Gross

1

Page 2: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Overview

1. Basics 2012-04-18

2. Materials 2012-04-25

3. Components 2012-05-02

4. Paraxial optics 2012-05-09

5. Properties of optical systems 2012-05-16

6. Photometry 2012-05-23

7. Geometrical aberrations 2012-05-30

8. Wave optical aberrations 2012-06-06

9. Fourier optical image formation 2012-06-13

10. Performance criteria 1 2012-06-20

11. Performance criteria 2 2012-06-27

12. Measurement of system quality 2012-07-04

13. Correction of aberrations 1 2012-07-11

14. Optical system classification 2012-07-18

2012-04-18

Page 3: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

9.1 Huygens principle of diffraction

9.2 Perfect point spread function

9.3 PSF with perturbations

9.4 Basics of Fourier optics

9.5 Image formation

9.6 Optical Transfer function

Contents:

Page 4: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Diffraction at the System Aperture

� Self luminous points: emission of spherical waves

� Optical system: only a limited solid angle is propagated, the truncaton of the spherical wave

results in a finite angle light cone

� In the image space: uncomplete constructive interference of partial waves, the image point

is spreaded

� The optical systems works as a low pass filter

objectpoint

sphericalwave

truncatedspherical

wave

imageplane

∆∆∆∆x = 1.22 λλλλ / NA

point

spread

function

object plane

Page 5: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Fraunhofer Point Spread Function

� Rayleigh-Sommerfeld diffraction integral,

Mathematical formulation of the Huygens-principle

� Fraunhofer approximation in the far field

for large Fresnel number

� Optical systems: numerical aperture NA in image space

Pupil amplitude/transmission/illumination T(xp,yp)

Wave aberration W(xp,yp)

complex pupil function A(xp,yp)

Transition from exit pupil to

image plane

� Point spread function (PSF): Fourier transform of the complex pupil

function

1

2

≈⋅

=z

rN

p

),(2),(),( pp yxWi

pppp eyxTyxAπ

⋅=

( ) ( ) ( )

pp

yyxxR

i

yxiW

pp

AP

dydxeeyxTyxEpp

APpp

''2

,2,)','(

+

⋅⋅=∫∫λ

ππ

''cos'

)'()(

'

dydxrr

erE

irE d

rrki

I ∫∫ ⋅−

⋅−=−

θλ

rrrr

rrr

Page 6: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

( )( )

0

2

12,0 I

v

vJvI

= ( )

( )0

2

4/

4/sin0, I

u

uuI

=

-25 -20 -15 -10 -5 0 5 10 15 20 250,0

0,2

0,4

0,6

0,8

1,0

vertical

lateral

inte

nsity

u / v

Circular homogeneous illuminated

Aperture: intensity distribution

� transversal: Airy

scale:

� axial: sinc

scale

� Resolution transversal better

than axial: ∆x < ∆z

Ref: M. Kempe

Scaled coordinates according to Wolf :

axial : u = 2 π z n / λ NA2

transversal : v = 2 π x / λ NA

Perfect Point Spread Function

NADAiry

λ⋅=

22.1

2NA

nRE

λ⋅=

Page 7: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Ideal Psf

lateral

Airy

axial

sinc2

aperture

cone image

plane

optical

axis

focal point

spread spot

Page 8: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Abbe Resolution and Assumptions

Assumption Resolution enhancement

1 Circular pupil ring pupil, dipol, quadrupole

2 Perfect correction complex pupil masks

3 homogeneous illumination dipol, quadrupole

4 Illumination incoherent partial coherent illumination

5 no polarization special radiale polarization

6 Scalar approximation

7 stationary in time scanning, moving gratings

8 quasi monochromatic

9 circular symmetry oblique illumination

10 far field conditions near field conditions

11 linear emission/excitation non linear methods

� Abbe resolution with scaling to λ/NA:

Assumptions for this estimation and possible changes

� A resolution beyond the Abbe limit is only possible with violating of certain

assumptions

Page 9: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

� Airy function :

Perfect point spread function for

several assumptions

� Distribution of intensity:

� Normalized transverse coordinate

� Airy diameter: distance between the

two zero points,

diameter of first dark ring'sin'

21976.1

unDAiry

⋅=

λ

2

1

2

22

)(

=

NAr

NAr

J

rI

λ

π

λ

π

'sin'sin2

θλ

πak

R

akrukr

R

arx ====

Perfect Lateral Point Spread Function: Airy

Page 10: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

log I(r)

r0 5 10 15 20 25 30

10

10

10

10

10

10

10

-6

-5

-4

-3

-2

-1

0

Airy distribution:

� Gray scale picture

� Zeros non-equidistant

� Logarithmic scale

� Encircled energy

Perfect Lateral Point Spread Function: Airy

DAiry

r / rAiry

Ecirc

(r)

0

1

2 3 4 5

1.831 2.655 3.477

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

2. ring 2.79%

3. ring 1.48%

1. ring 7.26%

peak 83.8%

Page 11: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

� Axial distribution of intensity

Corresponds to defocus

� Normalized axial coordinate

� Scale for depth of focus :

Rayleigh length

� Zero crossing points:

equidistant and symmetric,

Distance zeros around image plane 4RE

( ) 22

04/

4/sinsin)(

⋅=

⋅=

u

uI

z

zIzI o

42

2u

zNA

z =⋅⋅

π

'sin'22 un

RE

λ=

Perfect Axial Point Spread Function

Page 12: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Psf with Defocus

� Outside focus :

- Psf is broadened

- a system of rings

takes place

- symmetry around

image plane

Focus

image plane

Defocus

- 2 RE

Defocus

+ 2 RE

Defocus

+ 1 RE

Defocus

- 1 RE

Page 13: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Defocussed Perfect Psf

� Perfect point spread function with defocus

� Representation with constant energy: extreme large dynamic changes

∆∆∆∆z = -2RE ∆∆∆∆z = +2RE∆∆∆∆z = -1RE ∆∆∆∆z = +1RE

normalized

intensity

constant

energy

focus

Imax = 5.1% Imax = 42%Imax = 9.8%

Page 14: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Spherical aberration Astigmatism Coma

c = 0.2

c = 0.3

c = 0.7

c = 0.5

c = 1.0

Psf with Aberrations

� Zernike coefficients c in λ

� Spherical aberration,

Circular symmetry

� Astigmatism,

Split of two azimuths

� Coma,

Asymmetric

Page 15: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Comparison Geometrical Spot – Wave-Optical Psf

aberrations

spot

diameter

DAiry

exact

wave-optic

geometric-optic

approximated

diffraction limited,

failure of the

geometrical model

Fourier transform

ill conditioned

� Large aberrations:

Waveoptical calculation shows bad conditioning

� Wave aberrations small: diffraction limited,

geometrical spot too small and

wrong

� Approximation for the

intermediate range:

22

GeoAirySpot DDD +=

Page 16: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Definitions of Fourier Optics

� Phase space with spatial coordinate x and

1. angle θ

2. spatial frequency ν in mm-1

3. transverse wavenumber kx

� Fourier spectrum

corresponds to a plane wave expansion

� Diffraction at a grating with period g:

deviation angle of first diffraction order varies linear with ν = 1/g

0k

kv x

xx =⋅= λθ

[ ]),(ˆ),( yxEFvvA yx =

( ) ( )A k k z E x y z e dx dyx y

i xk ykx y, , ( , , )=

− +

∫∫

vk π2=

vg

⋅=⋅= λλθ1

sin

Page 17: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

� Arbitrary object expaneded into a spatial

frequency spectrum by Fourier

transform

� Every frequency component is

considered separately

� To resolve a spatial detail, at least

two orders must be supported by the

system

NAg

mg

λ

α

λ

λα

==

⋅=⋅

sin

sin

off-axis illumination

NAg

⋅=

2

λ

Ref: M. Kempe

Grating Diffraction and Resolution

optical

system

object

diffracted orders

a)

resolved

b) not

resolved

+1.

+1.

+2.

+2.

0.

-2.

-1.

0.

-2.

-1.

incident

light

Page 18: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

� A structure of the object is resolved, if the first diffraction order is propagated

through the optical imaging system

� The fidelity of the image increases with the number of propagated diffracted orders

0. / 1. order

0. / 1. / 2.

order

0. / 1. / 2. / 3. order

Resolution: Number of Supported Orders

Page 19: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Abbe Theorie of the Microscopic Resolution

� Diffraction of the illumination wave at the object structure

� Occurence of the diffraction orders in the pupil

� Image generation by constructive interference of the supported orders

� Object details with high spatial frequency are blocked by the system aperture and can not

be resolved

-1

1

object planesource

pupil plane

image plane

0

imaginglens

Ref: W. Singer

Page 20: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

� Imaging of a crossed grating object

� Spatial frequency filtering by a slit:

� Case 1:

- pupil open

- Cross grating imaged

� Case 2:

- truncation of the pupil by a split

- only one direction of the grating is

resolved

Fourier Filtering

Page 21: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

� Improved resolution by oblique illumination in microscopy

� Enhancement only in one direction

Oblique Illumination in Microscopy

Page 22: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Fourier Optical Fundamentals

� Helmholtz wave equation:

Propagation with Green‘s function g,

Amplitude transfer function, impulse response

� For shift-invariance:

convolution

� Green‘s function of a spherical wave

Fresnel approximation

� Calculation in frequency space: product

� Optical systems:

Impulse response g(x,y) is coherent transfer function, point spread function (PSF).

G(νx,νy) corresponds to the complex pupil function

� Fourier transform: corresponds to a plane wave expansion

),(*),','(),','( yxEzyyxxgzyxE −−=

g r rr r

ei k r r

( , ' )'

'r rr r

r r

=−

− −1

g x y zi

ze e

ikz

i

zx y

( , , )( )

= −− +

λ

π

λ2 2

),(),,(),,( yxyxyx vvEzvvGzvE ⋅=ν

)(22

),,( yx vvziikz

yx ezvvG+⋅+−

=λπ

dydxyxEyxyxgzyxE ∫ ∫∞

∞−

∞−

⋅= ),(),,','(),','(

Page 23: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

( )f

yxyxP

pp

pp⋅

+⋅−=

λ

π 22

),(

f

yv

f

xv

p

y

p

xλλ

== ,

Fourier Optical Field Propagation through a Lens

� Lens works as a pupil filter,

Phase:

Complex pupil function, transmission t

� Incident plane wave

Field in focal plane,

Far field, Fourier transform

� Image point coordinates correspond to the spatial frequencies

( )( )

∫∫+−

+

⋅⋅= pp

yyxxf

i

pp

yxf

i

dydxeyxAfi

efyxE

pp ''2

''

),(),','(

22

λ

πλ

π

λ

),(),(),( pp yxiP

pppp eyxtyxA ⋅=

Page 24: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Fourier Optics – Point Spread Function

� Optical system with magnification m

Pupil function P,

Pupil coordinates xp,yp

� PSF is Fourier transform

of the pupil function

(scaled coordinates)

( ) ( ) ( )[ ]∫∫

−⋅+−⋅⋅−

⋅⋅= pp

myyymxxxz

ik

pppsf dydxeyxPNyxyxgpp ''

,)',',,(

( )[ ]pppsf yxPFNyxg ,ˆ),( ⋅=

object

planeimage

plane

source

point

point

image

distribution

Page 25: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Fourier Theory of Coherent Image Formation

� Transfer of an extended

object distribution I(x,y)

� In the case of shift invariance

(isoplanasie):

coherent convolution of fields

� Complex fields are

additive

( )∫∫ ⋅= dydxyxEyxyxgyxE psf ),(',',,)','(

( )∫∫ ⋅−−= dydxyxEyyxxgyxE psf ),(',')','(

( ) ),(,)','( yxEyxgyxE psf ∗=

Page 26: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Fourier Theory of Coherent Image Formation

Page 27: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Fourier Theory of Incoherent Image Formation

objectintensity image

intensity

single

psf

object

planeimage

plane

� Transfer of an extended

object distribution I(x,y)

� In the case of shift invariance

(isoplanasie):

incoherent convolution

� Intensities are additive

dydxyxIyyxxgyxI psfinc ∫ ∫∞

∞−

∞−

⋅−−= ),()','()','(2

),(*),()','( yxIyxIyxI objpsfimage =

dydxyxIyyxxgyxI psfinc ∫ ∫∞

∞−

∞−

⋅= ),(),',,'()','(2

Page 28: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Fourier Theory of Incoherent Image Formation

objectintensity

I(x,y)

squared PSF,intensity-responseIpsf

(xp,y

p)

imageintensityI'(x',y')

convolution

result

object

intensityspectrum

I(vx,v

y)

opticaltransferfunction

HOTF

(vx,v

y)

imageintensityspectrumI'(v

x',v

y')

produkt

result

Fouriertransform

Fouriertransform

Fouriertransform

Page 29: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Comparison Coherent – Incoherent Image Formation

object

-0.05 0 0.05

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

incoherent coherent

-0.05 0 0.05

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

-0.05 0 0.05

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

-0.05 0 0.05

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

-0.05 0 0.05

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

-0.05 0 0.05

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

-0.05 0 0.05

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

-0.05 0 0.05

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

-0.05 0 0.05

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

-0.05 0 0.05

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

bars resolved bars not resolved bars resolved bars not resolved

Page 30: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Incoherent Image Formation in Frequency Space

� Incoherent illumination:

No correlation between neighbouring object points

Superposition of intensity in the image

� In the case of shift invariance

(isoplanasie):

Incoherent imaging with convolution

� In frequency space:

Product of spectra, linear transfer theory

The spectrum of the psf works as low pass filter onto the object spectrum

Optical transfer function

dydxyxIyyxxgyxI psfinc ∫ ∫∞

∞−

∞−

⋅−−= ),()','()','(2

),(*),()','( yxIyxIyxI objpsfimage =

dydxyxIyyxxgyxI psfinc ∫ ∫∞

∞−

∞−

⋅= ),(),',,'()','(2

[ ]),(),( yxIFTvvH PSFyxotf =

),(),(),( yxobjyxotfyximage vvIvvHvvI ⋅=

Page 31: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

( )

pppp

pp

vyvxi

pp

yxOTF

dydxyxg

dydxeyxg

vvH

ypxp

∫ ∫

∫ ∫∞

∞−

∞−

∞−

∞−

+−⋅

=2

22

),(

),(

),(

π

[ ]),(ˆ),( yxIFvvH PSFyxOTF =

pppp

pp

y

px

p

y

px

p

yxOTF

dydxyxP

dydxvf

yvf

xPvf

yvf

xP

vvH

∫ ∫

∫ ∫∞

∞−

∞−

∞−

∞−

−−⋅++

=2

*

),(

)2

,2

()2

,2

(

),(

λλλλ

Optical Transfer Function: Definition

� Normalized optical transfer function

(OTF) in frequency space

� Fourier transform of the Psf-

intensity

� OTF: Autocorrelation of shifted pupil function, Duffieux-integral

� Absolute value of OTF: modulation transfer function (MTF)

� MTF is numerically identical to contrast of the image of a sine grating at the

corresponding spatial frequency

Page 32: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

xp

y p

area of

integration

shifted pupil

areas

f x

yf

p

q

x

y

x

y

L

L

x

y

o

o

x'

y'

p

p

light

source

condenserconjugate to object pupil

object

objective

pupil

direct

light

at object diffracted

light in 1st order

Interpretation of the Duffieux Iintegral

� Interpretation of the Duffieux integral:

overlap area of 0th and 1st diffraction order,

interference between the two orders

� The area of the overlap corresponds to the

information transfer of the structural details

� Frequency limit of resolution:

areas completely separated

Page 33: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Optical Transfer Function of a Perfect System

� Aberration free circular pupil:

Reference frequency

� Cut-off frequency:

� Analytical representation

� Separation of the complex OTF function into:

- absolute value: modulation transfer MTF

- phase value: phase transfer function PTF

λλ

'sin u

f

avo ==

λλ

'sin222 0

un

f

navvG ===

=

2

000 21

22arccos

2)(

v

v

v

v

v

vvH MTF

π

),(),(),( yxPTF vvHi

yxMTFyxOTF evvHvvH ⋅=

Page 34: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

∆I Imax V

0.010 0.990 0.980 0.020 0.980 0.961 0.050 0.950 0.905 0.100 0.900 0.818 0.111 0.889 0.800 0.150 0.850 0.739 0.200 0.800 0.667 0.300 0.700 0.538

Contrast / Visibility

� The MTF-value corresponds to the intensity contrast of an imaged sin grating

� Visibility

� The maximum value of the intensity

is not identical to the contrast value

since the minimal value is finite too

� Concrete values:

minmax

minmax

II

IIV

+

−=

I(x)

-2 -1.5 -1 -0.5 0 1 1.5 2

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

x

Imax

Imin

object

image

peak

decreasedslope

decreased

minima

increased

Page 35: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

� Due to the asymmetric geometry of the psf for finite field sizes, the MTF depends on the

azimuthal orientation of the object structure

� Generally, two MTF curves are considered for sagittal/tangential oriented object structures

Sagittal and Tangential MTF

ψψψψ

y

tangential

plane

tangential sagittal

arbitrary

rotated

x sagittalplane

tangential

sagittal

gMTF

tangential

ideal

sagittal

1

0

0.5

0 0.5 1

νννν / ννννmax

Page 36: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

� Circular disc with diameter

D = d x Dairy

� Small d << 1 : Airy disc

� Increasing d:

Diffraction ripple disappear

Incoherent Image of a Circular Disc

Page 37: Design and Correction of optical Systems - iap.uni-jena.deand+correction... · Design and Correction of optical Systems Part 9: Fourier opticcal image formatioin Summer term 2012

Outlook

Next lecture: Part 10 – Performance Criteria I

Date: Wednesday, 2012-06-20

Contents: 10.1 Introduction

10.2 Geometrical aberrations

10.3 Wave aberrations

10.4 Point spread function and point resolution

10.5 Depth of focus