design of magnetic-field concentrators wednesday february 17th group: d mohammedzuned desai areio...
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Design of Magnetic-Field Design of Magnetic-Field ConcentratorsConcentrators
Wednesday February 17thWednesday February 17thGroup: DGroup: D
Mohammedzuned DesaiMohammedzuned DesaiAreio HashemiAreio Hashemi
Koji HirotaKoji HirotaMichael WongMichael Wong
OutlineOutline IntroductionIntroduction ApplicationsApplications ObjectivesObjectives MethodsMethods ResultsResults ConclusionConclusion Future WorkFuture Work Gantt ChartGantt Chart ReferencesReferences
IntroductionIntroduction
What are Magnetic Tweezers (MT)?What are Magnetic Tweezers (MT)? Scientific instrument used for studying Scientific instrument used for studying
molecular and cellular interactionsmolecular and cellular interactions Ability to apply known forces on paramagnetic Ability to apply known forces on paramagnetic
particles using a magnetic field gradientparticles using a magnetic field gradient One of the most commonly used force One of the most commonly used force
spectroscopy techniquesspectroscopy techniques• Atomic Force MicroscopyAtomic Force Microscopy• Optical TweezersOptical Tweezers
Advantages of MTAdvantages of MT
They do not have problems of sample heating They do not have problems of sample heating and photodamage that effects optical tweezersand photodamage that effects optical tweezers
Magnetic forces are orthogonal to biological Magnetic forces are orthogonal to biological interactionsinteractions
Offer the prospect of highly parallel single-Offer the prospect of highly parallel single-molecule measurements molecule measurements Hard to achieve with other single-molecule force Hard to achieve with other single-molecule force
spectroscopy techniquesspectroscopy techniques
ApplicationsApplications
The magnet configurations are relatively The magnet configurations are relatively easy to assembleeasy to assemble Combine force clamp properties with the Combine force clamp properties with the
ability to impose rotationability to impose rotation Ideally suited for the study of DNA topology Ideally suited for the study of DNA topology
and topoisomerasesand topoisomerases
ApplicationsApplications
http://www.biotec.tu-dresden.de/cms/fileadmin/research/biophysics/practical_handouts/magnetictweezers.pdf
ObjectivesObjectives
Design and fabricate Design and fabricate magnetic tweezers that magnetic tweezers that is capable of achieving is capable of achieving forces up to 100pNforces up to 100pN Current design can Current design can
achieve 2pNachieve 2pN Introduce illumination Introduce illumination
for bright-field for bright-field transmission transmission microscopymicroscopy
Ibrahim, George et al.
MethodsMethods
Using Finite Element Method Magnetics Using Finite Element Method Magnetics (FEMM) to predict the geometries of the (FEMM) to predict the geometries of the magnet and that will produce the largest magnet and that will produce the largest possible field gradients possible field gradients
Machine and assemble the design that will Machine and assemble the design that will produce the largest field gradients produce the largest field gradients
Calibrate the magnet so it is ready for data Calibrate the magnet so it is ready for data acquisitionacquisition
Rough DesignRough Design
MethodsMethods
FEMM MethodsFEMM Methods Testing for materialTesting for material
IronIron Mu metal Mu metal
Testing for shapeTesting for shape FlatFlat TipTip Flat TipFlat Tip
Testing for optimum distance between two Testing for optimum distance between two magnetsmagnets 11mmmm 33mmmm
FEMM ResultsFEMM Results
¼ inch
1.5 inch
1/8 inch
Results (Material)Results (Material)
Iron
Mu Metal
|B| vs. length
|B|/dl vs. length
Results (Shape)Results (Shape)
Tip
Flat Tip
|B| vs. length
|B|/dl vs. length
Results (Angle) Results (Angle)
Double Magnet Tip 2mm Separation
Double Magnet Tip 3mm Separation
|B| vs. length
|B|/dl vs. length
Results (Distance)Results (Distance)
Double Magnet FLAT 1mm Separation
Double Magnet FLAT 3mm Separation
|B| vs. length
|B|/dl vs. length
ConclusionsConclusions
Double flat magnet from mu metal gave Double flat magnet from mu metal gave best resultsbest results
If there is a sharp tip there is a huge If there is a sharp tip there is a huge gradient at the tip but the working distance gradient at the tip but the working distance is not feasibleis not feasible
The distance between the two magnets The distance between the two magnets effects the gradienteffects the gradient
Future WorkFuture Work
Orders for the magnet cores have been placedOrders for the magnet cores have been placed When core arrives we can begin fabrication and When core arrives we can begin fabrication and
eventually develop the magnetic tweezerseventually develop the magnetic tweezers
Contacted a company regarding the coiling of Contacted a company regarding the coiling of the magnetsthe magnets
Putting the entire set up togetherPutting the entire set up together Design a way for illumination of the sampleDesign a way for illumination of the sample
Progress ChartProgress Chart
ReferencesReferences 1) Neuman, Keri C, and Nagy, Attila. “Single-molecule force spectroscopy: optical 1) Neuman, Keri C, and Nagy, Attila. “Single-molecule force spectroscopy: optical
tweezers, magnetic tweezers and atomic force microscopy.” tweezers, magnetic tweezers and atomic force microscopy.” Nature Publishing GroupNature Publishing Group Vol. 5, NO. 6. June 2008.Vol. 5, NO. 6. June 2008.
2) Danilowicz, Claudia, Greefield, Derek and Prentiss, Mara. “Dissociation of Ligand-2) Danilowicz, Claudia, Greefield, Derek and Prentiss, Mara. “Dissociation of Ligand-Receptor Complexes Using Magnetic Tweezers.” Receptor Complexes Using Magnetic Tweezers.” Analytical ChemistryAnalytical Chemistry Vol. 77, No. Vol. 77, No. 10. 15 May. 2005.10. 15 May. 2005.
3) Humphries; David E., Hong; Seok-Cheol, Cozzarelli; Linda A., Pollard; Martin J., 3) Humphries; David E., Hong; Seok-Cheol, Cozzarelli; Linda A., Pollard; Martin J., Cozzarelli; Nicholas R. “Hybrid magnet devices fro molecule manipulation and small Cozzarelli; Nicholas R. “Hybrid magnet devices fro molecule manipulation and small scale high gradient-field applications”. United States Patent and Trademark Office, An scale high gradient-field applications”. United States Patent and Trademark Office, An Agency of The United States Department of Commerce. <http://patft.uspto.gov>. Agency of The United States Department of Commerce. <http://patft.uspto.gov>. January 6, 2009. January 6, 2009.
4) Ibrahim, George; Lu, Jyann-Tyng; Peterson, Katie; Vu, Andrew; Gupta, Dr. Sharad; 4) Ibrahim, George; Lu, Jyann-Tyng; Peterson, Katie; Vu, Andrew; Gupta, Dr. Sharad; Vullev, Dr. Valentine. “Magnetic Tweezers for Measuring Forces.” University of Vullev, Dr. Valentine. “Magnetic Tweezers for Measuring Forces.” University of California Riverside. Bioengineering Senior Design June 2009.California Riverside. Bioengineering Senior Design June 2009.
5) Startracks Medical, “Serves Business, Education, Government and Medical 5) Startracks Medical, “Serves Business, Education, Government and Medical Facilities Worldside.” American Solution. Startracks.org, Inc. CopyrightFacilities Worldside.” American Solution. Startracks.org, Inc. Copyright 2003. 2003. <http://images.google.com/imgres?imgurl=http://www.startracksmedical.com/<http://images.google.com/imgres?imgurl=http://www.startracksmedical.com/supplies/invertedmicroscope.jpg&imgrefurl=http://www.startracksmedical.com/supplies/invertedmicroscope.jpg&imgrefurl=http://www.startracksmedical.com/supplies.html&usg=__butCY2zWJa7nAkwkjiPxX_mFy0=&h=450&w=450&sz=24&hl=supplies.html&usg=__butCY2zWJa7nAkwkjiPxX_mFy0=&h=450&w=450&sz=24&hl=en&start=2&um=1&tbnid=XH6gnQuJLS7bRM:&tbnh=127&tbnw=127&prev=/imagesen&start=2&um=1&tbnid=XH6gnQuJLS7bRM:&tbnh=127&tbnw=127&prev=/images%3Fq%3Dinverted%2Bmicroscope%26hl%3Den%26sa%3DN%26um%3D1>%3Fq%3Dinverted%2Bmicroscope%26hl%3Den%26sa%3DN%26um%3D1>