detecting aluminum in drinking water erica sandoval dr. henry altmiller
TRANSCRIPT
Detecting Aluminum in Drinking WaterDetecting Aluminum in Drinking Water
Erica SandovalDr. Henry Altmiller
Hypothesis & GoalsHypothesis & Goals
Low levels of Aluminum in drinking water can be detected using a Photometric technique and GC-MS.
•Fluorescence Method
•GC-MS
MethodologyMethodology
1. O-Phenanthroline (15mM) and Be2+ (0.1M)
2. Sodium Acetate Buffer Solution
3. Lumogallion
4. Heated
5. Triton
6. Measure Fluorescence
ProblemProblem
Detecting 2x10-3 MNeeded to Detect 7.413x10-7 M
Variables ChangedVariables Changed
Sodium Acetate Buffer
Time
Triton
Lumogallion
Temperature
Neat vs. DilutedNeat vs. Diluted
Triton 1% v/v
TritonTriton
Triton
1% v/v
1% v/v
Sodium Acetate BufferSodium Acetate Buffer
100uL pH=4.03
200uL pH=4.49
300uL pH=4.97
pH=4.49pH=4.97
pH=4.03
TimeTime
30 minutes
45 minutes
60 minutes
60 min
45 min30 min
TemperatureTemperature
25oC
4oC
25oC
4oC
LumogallionLumogallion
100 uL = 2.85x10-6M
200 uL = 5.67x10-
6M 300 uL = 8.46x10-6M
5.67x10-6 M
8.46x10-6 M
2.85x10-6 M
Optimum Conditions FoundOptimum Conditions Found
Excess lumogallionNeat Triton1 hourpH 54oC
All Aluminum Chelated
Why Fluorescent Intensity ImprovesWhy Fluorescent Intensity Improves
More Viscous
Less Collisions
More time to form complex
Testing 7.413x10Testing 7.413x10-7-7M SolutionM Solution
Different color Less Fluorescent Intensity
pH=4.96
pH=4.83
pH=4.77
What About the Instrument?What About the Instrument?
Slit Width
– Excitation– Emission
AccomplishedAccomplished
Found Optimum Conditions
Learned Instrument
ConclusionConclusion
Test Drinking Water
Begin GC-MS Technique
Different Water Source