determination of albumin concentration by mip qcm sensor
DESCRIPTION
Determination of Albumin Concentration by MIP QCM Sensor. Speaker : Thong-Yueh Lin. Loading curves of different MIP thin film. The amount of adsorption for different MIP thin films. Selectivity of DMAPMA-TMPTMA thin film. Conclusions. The protein mixture could be analyzed by HPLC. - PowerPoint PPT PresentationTRANSCRIPT
Determination of Albumin Determination of Albumin Concentration by MIP QCM SensorConcentration by MIP QCM Sensor
Speaker : Speaker : Thong-Yueh Lin Thong-Yueh Lin
Loading curves of different MIP thin filmLoading curves of different MIP thin film
Time (sec)
0 1000 2000 3000 4000 5000 6000 7000
Fre
qu
ence
(H
z)
-250
-200
-150
-100
-50
0
50
DMAPMA-TMPTMADMAPMA-PETTA filmDMAPMA-TEGDMA film
Sample nameDMAPMA-TEGDMA
DMAPMA-TMPTMA
DMAPMA-PETTA
Mass of coating (g) 1.79*10-6 4.119*10-7 1.015*10-5
Adsorption amount (g)
7.10*10-8 8*10-8 5.295*10-8
Adsorption amount (g) per gram MIP
0.04 0.1942 0.005
The amount of adsorption for different The amount of adsorption for different MIP thin filmsMIP thin films
Selectivity of DMAPMA-TMPTMA thin filmSelectivity of DMAPMA-TMPTMA thin film
Cytochrome CLysozym
eAlbumin Myoglobin
Adsorption amount mass ratio of MIP
160 1 1942 30
Adsorption amount mass ratio of non-MIP
13 1 249 86
ConclusionsConclusions
1.1. The protein mixture could be analyzed by HPLC.The protein mixture could be analyzed by HPLC.
22.The MIP thin film coated on Au-OH electrode .The MIP thin film coated on Au-OH electrode
indicated the highest albumin adsorption.indicated the highest albumin adsorption.
3.The MIP thin film coated on Au-NH3.The MIP thin film coated on Au-NH22 electrode electrode
indicated the shortest stable time.indicated the shortest stable time.
= albumin : cytochrome c : myoglobin : lysozyme= albumin : cytochrome c : myoglobin : lysozyme
= 1.4 : 1.0 : 1.5 : 3.1= 1.4 : 1.0 : 1.5 : 3.1
22. Selectivity mole ratio of MIP. Selectivity mole ratio of MIP
= albumin : cytochrome c : myoglobin : lysozyme= albumin : cytochrome c : myoglobin : lysozyme
= 9.1 : 2.9 : 2.8 : 1= 9.1 : 2.9 : 2.8 : 1
ConclusionsConclusions
4.The DMAPMA-TMPTMA MIP thin film 4.The DMAPMA-TMPTMA MIP thin film
showed the highest albumin adsorption showed the highest albumin adsorption
amount.amount.
5.The Selectivity of DMAPMA-TMPTMA thin 5.The Selectivity of DMAPMA-TMPTMA thin
film coated on QCM was cytochrome C: film coated on QCM was cytochrome C:
lysozyme: albumin: myoglobin= lysozyme: albumin: myoglobin=
160:1:1942:30. 160:1:1942:30.
..