Development and characterization of the common bean diversity

panel (ADP)

BREEDING LOCALLY-ADAPTED PULSE CROPS FOR ENHANCED YIELD AND SEED QUALITIES: AN INTEGRATED, OUTCOME-BASED PLAN FOR ARS

• Karen Cichy ARS East Lansing, MI• Mike Grusak ARS Houston, TX • Phil Miklas ARS Prosser, WA• Talo Pastor-Corrales ARS Beltsville, MD• Tim Porch ARS Mayaguez, PR

James Beaver UPR Mayaguez, PRConsuelo Estevez UPR Mayaguez, PR

Gail Wisler ARS-NPLRoy Scott ARS-NPL

The main goal of the project is to increase common bean production in Sub-Saharan Africa, through the development of germplasm resources, molecular tools, and scientific knowledge.

1. Develop an Andean Diversity Panel (ADP) comprised of 300-500 dry bean lines for genetic characterization of targeted traits and identi- fication of breeding materials.

2. Identify and develop Andean germplasm with improved biological nitrogen fixation (BNF) and improved production on degraded low input soils.

3. Improve tolerance to abiotic stresses-drought and heat.4. Identify Andean common bean cultivars with disease

resistance genes for the control of the most widespread and yield-reducing diseases in target countries.

OBJECTIVES

5. Determine the phenotypic variation of seed phytic acid , flavonoids, and cooking time.

6. Determine the phenotypic variation and genetic control of seed mineral concentrations, pod mineral partitioning, and pod dry matter partitioning in Andean beans.

OBJECTIVES

Objective 1: Develop Andean Diversity Panel

East African landraces; 120

N. American (BeanCAP); 89

CIAT Africa Misc; 74

CIAT and US core collection; 65

East and South African varieties;

34

Caribbean; 20

Angola; 16Ecuador; 12 Non nods; 4

Andean diversity panel 434 entries

Increase of the ADP Panel

Subsets Increased in:

Prosser, WashingtonIsabela, Puerto RicoSouth Africa

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Purgatory plot yield (kg ha-1) - Top 25 lines - Prosser, WA 2012

Objective 2: Identification of ADP germplasm efficient under low fertility conditions

Morogoro, Tanzania

Trial of 95 landraces in Tanzania, 2012Results from top 6 lines

pods plant

seeds pod sd wt Yield

Shoot dry wt Nodule Rust CBB ALS

Virus BCMV

Sub ID Name No. No.g 100 sds kg ha g No.

TZ-4 KILOMBERO 12.6 3.7 49.5 1655 7.40 27.5 1.0 3.0 1.0 1.8

TZV-58 KIJIVU 10.3 5.0 44.0 1373 7.33 14.5 2.0 1.0 1.0 2.5

TZ-14 KIANGWE 10.8 4.2 39.8 1366 5.20 9.0 1.0 2.0 3.0 1.0

TZV-73 MASUSU 11.4 3.6 48.1 1334 8.25 17.5 2.0 1.0 2.0 1.0

TZV-41 MRONDO 14.9 4.6 37.4 1325 6.31 14.3 1.0 1.0 1.0 1.0

TZ-7 BUKOBA 15.8 5.3 36.4 1323 5.12 20.8 1.0 2.0 1.5 1.0

Correlation 0.47**

Objective 3: Evaluate Abiotic Stress ResponseTop 25 performers for Drought – Othello, WA 2012

Drought Intensity Index = 40%

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Yiel

d (k

g ha

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eom

ean)

Objective 4: Identify disease resistant germplasm within the ADP

1 13 25 37 49 61 73 85 97 109121133 145157169181193205 217229 241253265 2770.0

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Limited Common bacterial blight (CBB) Resistance in ADP

Correlation coefficient between strains: 0.92

Lines

1-9

Scal

e, C

BB

Blue- 3353Red- 484A

ADP Sub-ID Genotype 3353 484A SAP6 SU91

635 BCV-508 OAC Redstar 4.0 2.5 + -

430 PR-4 PR1013-3 4.5 2.5 + -

432 PR-6 PR0637-134 4.5 2.5 + -

435 PR-9 RM-05-07 4.3 2.8 + -614 BC-315 ND061106 4.0 3.0 + -

32 TZ-32 RH No. 21 4.8 2.5 + -607 BC-154 NY 105 4.8 3.0 + -96 AF-1 Rojo 5.5 2.8 + -

617 BC-347 Red Rider 9.0 8.0 - -618 BC-351 AC Elk 8.5 7.0 - -97 AF-2 Bilfa 4 9.0 8.8 - -

VAX 6 1.7 1.3 + +Morales 9.0 9.0 - -

Mean 8.6 7.7LSD 0.8 1.1

Response to strains 484A and 3353 of CBB285 lines; 7 R., 16 Int., 262 S.

ADP Response to NL3 Strain of BCMNVOut of 303 lines: 37 with I gene and 3 with bc3 gene

ADP Sub-ID Source Genotype NL3 Response ENM- (bc3) SW13 (I)614 BC-315 WM1131-159 ND061106 Necrosis (-) (+)635 BCV-508 OT1127-57 OAC Redstar Necrosis (-) (+)607 BC-154 WM1131-157 NY 105 Necrosis (-) (+)605 BC-152 WM1131-145 1132-V96 Necrosis (-) (-)118 AF-23 OT1127-26 Werna Necrosis (-) (+)427 PR-1 J.Beaver Badillo Necrosis (-) (+)434 PR-8 J.Beaver PR0737-1 Necrosis (-) (+)121 AF-26 OT1127-30 Kranskop HR-1 Necrosis (-) (+)225 CC-22 05IS-0227 G 6415 Necrosis (-) (+)599 BC-76 OT1127-44 Isles Necrosis (-) (+)122 AF-27 OT1127-31 Kranskop Necrosis (-) (+)113 AF-18 OT1127-17 OPS-RS4 Necrosis (-) (+)126 AFC-7 SELIAN 05 Necrosis (+) (-)632 BCV-505 OT1127-27 TARS HT 1 Necrosis (-) (+)618 BC-351 WM1131-148 AC Elk Necrosis (-) (+)629 BCV-502 OT1127-15 H9659-27-10 Necrosis (-) (-)606 BC-153 WM1131-123 NY 104 Necrosis (-) (-)623 BC-399 WM1131-163 Drake Necrosis (-) (+)624 BC-401 OT1127-55 Dolly Necrosis (-) (+)630 BCV-503 OT1127-18 H9659-23-1 Necrosis (-) (-)26 TZ-26 OT1122-111 Black Wonder Necrosis (-) (-)51 TZV-52 OT1122-1 RH No. 3 Necrosis (-) (-)55 TZV-56 OT1122-16 KABUKU Necrosis (-) (-)97 AF-2 OT1127-48 Bilfa 4 Necrosis (-) (-)

117 AF-22 OT1127-24 A483 Necrosis (-) (-)123 AF-28 OT1127-33 Jenny Necrosis (-) (+)452 EC-6 E.Peralta INIAP 425 Necrosis (-) (+)456 EC-10 E.Peralta INIAP 480 Necrosis (-) (+)517 AG-10 2010-TP20 Carioca, Kibala Necrosis (-) (+)600 BC-100 OT1127-46 K07921 Necrosis (-) (+)608 BC-175 WM1131-118 UI-51 Necrosis (-) (+)609 BC-248 WM1131-146 K-407 Necrosis (-) (+)610 BC-265 WM1129-121 G-122 Necrosis (-) (-)616 BC-344 WM1131-134 OAC Lyrick Necrosis (-) (+)619 BC-367 WM1131-158 UCD 0906 Necrosis (-) (+)622 BC-378 WM1131-112 UCD 0701 Necrosis (-) (+)628 BCV-501 OT1127-14 H9659-27-7 Necrosis (-) (+)

Ashy stem blight responseusing Band-aid method

Combined Results Response Lesion length Notes18 Res., 1-3 0-4 cm

155 Int., 4-6 5-8 cm88 Susc., 7-9 >= 9 cm or dead

Objective 5: Determine the phenotypic variation of seed phytic acid, flavonoids, and cooking time

01/24/1900

01/30/1900

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Freq

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minCooking Time on 100 ADP lines

Elemental analysis of 40 Andean Entries – SeedsElements Prosser Range Fold Range

(Prosser)Ca (mg/g DW) 0.7 – 2.7 4.1 xK (mg/g DW) 11.3 – 19.9 1.8 xMg (mg/g DW) 1.4 – 2.9 2.1 xP (mg/g DW) 4.2 – 6.8 1.6 xS (mg/g DW) 1.9 – 2.9 1.5 x

Cu (µg/g DW) 6.5 – 10.8 1.7 xFe (µg/g DW) 63.2 – 99.6 1.6 xMn (µg/g DW) 10.9 – 19.6 1.8 xMo (µg/g DW) 0.4 – 4.6 11.0 xNi (µg/g DW) 0.6 – 4.7 7.8 xSe (µg/g DW) not detectedZn (µg/g DW) 30.0 – 47.5 1.6 x

Potential impact: Broad diversity exists for seed mineral concentrations.

Association mapping with the ADP should lead to the

identification of seed mineral QTLs for breeders.

Objective 6: Determine the phenotypic variation and genetic control of seed mineral concentrations

Diversity Analysis- Based on 73 informative SNPs

0.1

G4564IZ21

TZV 71TZV 83

TZV 90 TZV 87TZV 81G18356

UCD0701UCD0906BCV 500TZV 72TZV 51MyasiAF 24UCD0233G51496A

TZ 1PI310511

TZ 35TZ 39TZ 36TZ 9 TZ 8

INIAP481INIAP418

G20729G13092

TZ 37TZ 29

ICA Quimbaya

TZV 99TZ 38

USCR 9USCR 7

TZ 10

TZ 31

TZV 43TZ 17TZ 15TZV 61TZ 6 TZ 18

TZ 13

TZ 28

TZ 27

Gurabo5

ColoradodelPaisCAL 143UCD0405EtnaRed RiderHooterDolly

TaylorHortCran 09TZV 57TZ 21TZ 20

TZ 25

TZ 11TZ 5TZ 3TZ 23TZ 16TZV 66TZ 22

TZV 97TZV 96

UI 686

Krimson

USCR CBB 20

Capri

UI 51

Cardinal

Bellagio

TZ 30

TZ 26PompadourBLargaComercial

MICran

TZV 63TZV 56TZV 58Fernando

PR1013 3

IndJamaicaRedTZV 98TZV 68TZV 65TZV 73TZV 59TZV 49CDRK

UC RedKidneyCharlevoix

BCV 507

ErvihaG 122TZV 62TZV 55

TZV 50TZV 82 TZV 47

Camelot

USWK CBB 17Chinook2000OAC Redstar

AC CalmontUSDK CBB 15

USDK 4

Montcalm1132 V96

Red Kote

RedKanner

18 V96

Litekid

Drake

Red Hawk02 385 14

TZV 88

Isles

PR9745 232

G51493A

Hutterite

TZ 7

TZ 32AF 1

TZV 67

TZ 24

G51137AAF 30

TZV 44TZ 33TZ 34TZ 2

TZ 19G23086Katarina K

G23093G22455

TZV 74TZV 77TZV 48

AG 11

UCD0801

INIAP420

TZV 89TZV 94

TZ 4

Ohlio deperdiz

TZV 79

TZV 85TZV 60TZV 92TZV 91TZV 86TZV 84TZV 75TZV 70TZV 69TZV 64TZV 93TZV 52TZV 53TZV 45

Nuvolari

K 407

Royal RedLisaKardinalKamiakin

Silver Cloud

Badillo

ICB 12

A 55G4445A

INB841

OAC CygnusBuster

SB DT1

AC CompassUSPT ANT 1ND060197

G51495A BeniquezIZ102

TZV 95

TZV 40

K 42

Blush K 59

Fiero

K07921BelugaMajesty

JaloEEP558

VA 19

UC Nichols

Wallace

ND061106AC Elk

OAC LyrickIsabella

FoxFire

Red KloudLarkUSWK 6 1062 V98Pink Panther

SacramentoLassenUSLK 1

CELRK NY105NY104UC WhiteKidney

UC LindenTZ 14

TZV 54TZV 42TZV 41

ADP Currently in the process of being SNP genotyped with 6,000 BeanCAP SNPs for AM Analysis

Sharing of ADP population Development of Access Database

Concluding Comments

• Initial findings in ADP– Limited disease resistance– Promising efficiency under low fertility, and for BNF– Useful range of phytonutrient concentration and cooking times

• ADP dataset will be important:– For mapping new traits/developing markers in Andeans– Establishing an initial baseline of African germplasm in research and

breeding efforts– Determining research/breeding priorities focused on Africa

Acknowledgements: