development of a new management strategy for...

C E N T R E M O N D I A L D E L ’ I N N O V A T I O N R O U L L I E R

Development of a new management strategy

for limiting Nematodes damages in soybean production

Dr JEAN CLAUDE YVIN

OUTLINE

2. BACKGROUND

3. LAB and GREENHOUSE EVALUATIONS

4. FIELD TRIALS RESULTS

5. CONCLUSIONS

1. ROULLIER RESEARCH FACILITIES

CMI-Roullier Group : in few words

Saint-Malo, France

The Roullier Group

8000 Employees

(68% outside France)

Present in 114 Countries

3000 Sales Representatives

2 Billion € Turnover

French Agro supply Company Founded in 1959 by Daniel Roullier

Specializes in the Production and Processing of Nutrients and Feeds for Plants, Animals and Humans

Agronomic and Experimental Infrastructure

Growth Chambers

Extraction, Formulation Lab

Mass Spectrometry Lab

Molecular biology Lab

Lab Facilities Phenotyping Platform Greenhouses

o Root and Shoot Imaging System (non destructive

screening)

o Different Cultivation System

o Different Model Species

The Research Center - CMI-RoullierResearch area :

Plant and Animal Nutrition

✓ NPKS Plant Nutrition

✓ Biostimulation

✓ Biocontrol and Abiotic Stresses

✓ Natural Extract

Soil expertise

OUTLINE

2. BACKGROUND



5. CONCLUSIONS


Nematodes

2. BACKGROUND

o Phylum Nematoda

o Roundworms

o Microbial Feeders

o (90% plus)

o Animal Parasitic

o Human Parasitic

o Insect Parasitic

o Plant Parasitic

2. BACKGROUND

Most kinds of soil nematodes do not parasitize plants, but are beneficial in the

decomposition of organic matter

2. BACKGROUND

Plant-Parasitic Nematodes

o Over 6000 known species

o Present in all ecological niches

o Attacks almost all plants

o Annual crops losses are estimated at 8,8% -14,6% of

total crop production and 100-157 billions USD worldwide

(S.K Sing, 2013)

2. BACKGROUND

Plant-parasitic

nematodes all have

stylets (spears) that

penetrate cells

and withdraw the

contents

2. BACKGROUND

Nematode Damages

o Yield loss

o Stunting / Yellowing

o Water deficit and wilting

o Nutrients deficiencies

o Some direct damages ( knots on roots , misshapen potatoes …

2. BACKGROUND

Classical Nematode

foliar damages symptoms

2. BACKGROUND

Root-Knot Nematode Damages

Photo courtesy G. S. Abawi, © The American Phytopathological Society.

2. BACKGROUND

Source : FAOSTAT 2014

RANK COUNTRIES PRODUCTION

(In millions tonnes )

Cultivated areas

( millions ha)

WORLDWIDE SOYBEAN PRODUCTIONS

2. BACKGROUND

Ano Safra* Produção*

(t)

Perda estimada em

10,6%**

(t)

R$/saca

60 kg

Prejuízos

(R$)

16/17 114.000.000 12.084.000 60,00 ~ 12,084 bilhões

Estimation of damages dues to Nematodes on Soja in Brasil 2016/17

*Fonte: CONAB 2017 ** SASSER & FRECKMAN (1987)

2. BACKGROUND

Frequency of occurrence of nematode species , highlighting the frequency ofPratylenchus dihystera and Scutellonema brachyurus in soils (A) and roots (B)

PlosOne, August 2019

2. BACKGROUND• Nematodes diseases have a worldwide importance for several economic crops such as soybean

• Frequently new pathogens arise as emergent diseases

2. BACKGROUND

Management Methods

✓Exclusion✓Sanitation✓Rotation ✓Plant Resistance ✓Fallow ✓Solarization✓Nematicides✓Biocontrol products

OUTLINE

2. BACKGROUND



5. CONCLUSIONS



Evaluation of new biologically active Seaweed

extracts on the mobility of Meloidogyne javanica

FC 200 : A well-characterised Brown Algae Extract

Source: French Natural Brown

Seaweeds harvested in Britanny

Process of production:A well controlled and safe

process

CMI : analytical Biochemistry

(In: Plant-Cyst Nematode and Plant-Root-knot Nematode Interactions. A. Niebel, G. Gheysen and M. Van Montagu Parasitology Today, vol. 10 no. 11, 1994)

Biological cycle of root -knot nematodes Meloidogyne sp

Mobile forms : Juveniles L2 mobiles


Screening of NÉMATICIDE/NÉMATOSTATIC ACTIVITIES

ProduitH2O

Recovered

Nematodes +++

+

-

Nematostaticou

Nematicide activity

REVERSIBILITY

Recovered

Nematodes++++ - Selection of

Activity

H2O H2O

Nématostatic Nématicide

Nématodes /Sieves

More than 100 natural products compositions have been tested with ELISOL

0

100

200

300

400

500

600

Concentration FC200 (mM)

Série 1Série 2

Number of M. javanica recoveredin presence of FC200 after 21 hours

-50

0

50

100

150

200

250

300

350

400

450

passage 1 Passage 2 Passage 3

0

6 mM

60 mM

Number of M. javanica recovered

FC200 H2O24h

H2O48h

Reversibility of inhibition

FC200 shows nematostatic effects at low concentrations

• RESULTS


activ transport

kinetics of mobility M. JAVANICA in soil affected by FC200

Soil+ FC200+ Nématodes

Incubation

H2O H2O

Number of Nematodes recovered f(t)

Inhibition of mobility of juveniles of M. javanica in soilReversibility of the inhibition 4/5 days after

0

50

100

150

200

250

300

350

0 20 40 60 80 100 120 140 160N

om

bre

s d

e n

ém

ato

de

s e

n s

ort

ie d

e

colo

nn

eTemps (heures)

Témoin

FC200 780ppm

FC200 7800 ppm

Vydate 1000ppm

Number of mobile

nematodes f(t)

• RESULTS


UFRRJ e INTA

screening of a biostimulant on the mobility of the root-knot nematodes Meloydogine spp.

Professor R. l.louro BerbaraDepartement de SolosUFRRJ


T0 – Control

T1 – with nematodes inoculum without nematicides

T2Q – chemical control (inoculation+ Abamectina in seed treatment

T3D – biological control (inoculation + F in seed treatment°

T4 – nematodes inoculation+ (100% C)

T5 – nematodes inoculation+ ( 50% C y 50% F)

T6 – nematodes inoculation+ ( 70% C y 30% F)

T7 – nematodes inoculation+ (30% C y 70% F)

T8 – nematodes inoculation+ (100% F)

Treatments


Evaluation of infestation


Gráfico da media de Peso fresco de raiz (PFRaiz) (LSD< 0.05)

• RESULTS


Treatment 1with nematodes inoculum, without nematicide

Treatment 2With nematode inoculum and Nematicide

• RESULTS


FC200 DOSE 1 FC200 DOSE 2

• RESULTS


Average number of Galls per root fresh weight (g) (LSD p<0.05)

• RESULTS


Screening of FC 200 on Soya in potsIn presence of

Meloidogyne javanica Pratylenchus brachyurus

Heterodera glycines

Professor Pedro SUARES


3.UNESP

SOY PRODUCTIVITY IN SOIL WITH

Meloidogyne Javanica NEMATODES

Soy with SSP fertilizer

(with nematode)Soy with NP Plus fertilizer

(with nematode)


Pratylenchus Brachyurus NEMATODES



(with nematode)

SSP* Simple superphospate

UNESP

Heterodera glycines



(with nematode)


Heterodera glycines NEMATODES


OUTLINE

2. BACKGROUND



5. CONCLUSIONS


Aerial photo

4. FIELD TRIAL RESULTS

Farm “Arco Íris”- MT/Brasil



(with nematode)


OUTLINE

2. BACKGROUND



5. CONCLUSIONS


Soil nematode abundance generally decreases with increasing

depth and distance from plants, as many soil nematodes are

largely concentrated in the rhizosphere. Ingham et al. (1985)

Localisation of NEMATODES

0

15 CM

https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/nematode

https://www.sciencedirect.com/topics/earth-and-planetary-sciences/nematode

https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/rhizosphere

FC200 marine Biostimulant

Enhancer of Soybean root Biomass( posItive effect in the competion between root growth

and nematode attacks

Increase the nutrition of the plant

Foster development of beneficial microrganisms

in the RhizosphereEffect of organic-complexed superphosphates on microbial

biomass and microbial activity of soil

C. Giovannini & J. M. Garcia-Mina & C. Ciavatta & C. Marzadori

August 2012

ÚNICO FERTILIZANTE

DESENVOLVIDO PARA

ÁREAS COM PRESENÇA

DE NEMATOIDES

NP+

FERTILISATION NP+CONTROL

5. CONCLUSIONS

Preserve soil Biodiversity and Ecosystem

Increase soil microorganisms in the Rhizosphere

Maintain or increase soil fertility

Give a natural boost to the root system

Increase the yield in soybean production

A new innovative Fertiliser

Muchas Gracias por su atención

Dr JEAN CLAUDE YVIN

[email protected]

development of a new management strategy for...

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