Development of a TechnicalProgram in Stem Cell Science:

Responding to an Emerging Need

National Science FoundationAdvanced Technological Education

DUE 1104210

Madison College

• 2012 Centennial Celebration

•Over 140 career paths serving more than 45,000 students, including many of the fastest growing occupations identified by the WDWD.

•12 Campuses (8 in Madison, 4 Regional).

•Greater than 90% placement rate for graduates within 6 months.

•Technical colleges generate nearly $7 billion in economic benefits for Wisconsin each year.

Facilities Expansion

Health Education

Ingenuity Center

Protective Services

Biotechnology in Wisconsin

• Between 2004 and 2009 bioscience employment in Wisconsin grew by nearly 3% in contrast to the rest ofthe economy that shrunk by > 3%

• The average bioscience worker has earnings that are 64% higher than the earnings of a typical Wisconsin employee

• More than 640 Wisconsin bioscience businesses have created nearly 24,000 private sector jobs with atotal economic impact of close to $7 billion

•Reported industry revenues in 2005 totaled $974,000 and $36.9 million in 2007. In the US, by 2016, stem cell company revenues are projected to exceed $8.5 billion and $16-20 billion by 2020.

•The state of Wisconsin has invested more than $94 million, in addition to private investments to promote growth in the stem cell and regenerative biology sector.

•California Institute of Regenerative Medicine (CIRM) $3 billion in Grants.

The Bioeconomic impact of Stem Cell Science

Stem Cells & Regenerative BiologyAt Madison College

• In 2007, Madison College piloted our first human stem cell course.

•In 2011, NSF awarded $851,454 to develop a technical education program in Emerging Stem Cell Technologies.

•40 Advisory Board Members: Stem Cell Industry, UW Madison SCRMC, UW Waisman Center for Developmental Disorders, and Morgridge Institute for Research / Wisconsin Institute for Discovery.

Development of a Technical Program in StemCell Technologies: Responding to an Emerging Need (NSF DUE 1104210)

OBJECTIVES:

I. Development of a 2-semester certificate programemphasizing workforce training in Stem Cell Technologies.

II. Develop educational materials in stem cell technologies fordissemination at the local, regional, and national levels.(web-based distribution, instructional videos, manuals)

III. Promote the growth of Stem Cell Programs in other Colleges& Universities throughout the nation

Introduction to human Stem Cell Methods: Basic Culture and Characterization

Course Competencies:Characterization of hESCs• Chromosomal analysis, florescence microscopy

• Media Formulation (Feeder-dependent/xeno-free media)

•Generation of Cell Aggregates (EBs)

hESC H9 DAPI StainFor metaphase chromosomes

hESC H9 Cell Aggregates:Embryoid formation

Scheduled Meeting time: Lecture: Monday 5:00pm to 5:50pm Laboratory: Monday/Thursday 6:00pm-8:50pm

Target audience: AAS Biotechnology, BS in Biological Sciences at or nearing completion,

Post-Baccalaureate, Graduate Students, Laboratory Staff.

Human Stem Cell Technologies Certificate Program: 4 Courses (8 Credits)

SEMESTER 1: Introduction to human Stem Cells- Basic Culture and Characterization

Course Competencies:• Stem Cells and Bioethics

• Aseptic techniques and routine maintenance of PSC cell cultures.

• Basic techniques for culturing hES (H9) cells:thawing, plating, feeding, passaging, and cell banking.

Pluripotent Stem Cell colony pre-split (10x)

Pluripotent Stem Cell colony pre-split (2.5x)

Course Competencies:• hES culture systems: Feeder dependent/independent.

hESC H9 Colony onMEF-feeder layer (20x)

hESC H9 Colony – Feeder independentOn Matrigel (10x)

SEMESTER 1: Introduction to human Stem Cells- Basic Culture and Characterization

Course Competencies:• hESC (H9) and iPS (iMR90-4) Cell Differentiation

Spontaneous & Directed Differentiation of Adherent Cells and Cell Aggregates

•Immunoflourescence Microscopy & Molecular Analysis ofPSC-derived differentiated cells

SEMESTER 2: Advanced human Stem Cells- Differentiation & Applications

HepatocyteDifferentiation

Neural RosetteDifferentiation

CardiomyocyteDifferentiation

Advanced Cell Culture Education Suite (ACCES)

•2744 sq.ft. Animal Cell Culture Facility.•Construction completed and fully operational, January 2012.

Advanced Cell Culture Education Suite (ACCES)

Molecular Wet Lab Cell Culture Imaging & Microscopy

Neuronal Differentiation

2013 Biopharmaceutical Technical Center Institute

STEM CELL SYMPOSIUM

Summary : Stem Cell Education

With NSF ATE support, we have developed a technical training program in Human Stem Cells to

address the emerging demands of the global bioeconomy. Programming also includes the

creation of 1-day workshops & a 5-day Short Course

Stem Cells & Regenerative BiologyIn Madison, Wisconsin

Thank you!

Supported by the National Science Foundation AdvancedTechnological Education Project Grant DUE 1104210

Awarded to Madison College July 2011

Contact Information

•Lisa Seidman, Ph.DPI, Biotechnology Program Director(608) 246-6204lseidman@matcmadison.edu

•Thomas Tubon, Ph.D.Project Director/Co-PI, NSF Stem Cell Program at Madison College(608)246-6875tubon@matcmadison.edu

•Jeanette Mowery, Ph.D.Co-PI, Facultyjmowery@matcmadison.edu