Developmental Anatomy Preformation vs Epigenesis: A history. Primary Germ Layers and early organs. Karl von Baer and his laws. Fate Mapping Evolutionary Embryology.

Aristotle: First biologist? The Generation of Animals (350 BCE) Noted Variations of life cycle themes:

Some born of Eggs (oviparity) Some from live birth (viviparity) Some from eggs, but hatch in mother

(ovoviviparity). Holoblastic- whole egg divided into smaller

cells Meroblastic- half egg divides into smaller cells;

rest is yolk sac.

Cell Division differs amongst species

Ex ovo omnia- Willaim Harvey 1651

Preformation Belief that everything is

in egg and/or sperm in smaller form.

Overtime, organs and essentially the organism grows.

Marcello Malphigi- studies chick embryos..notes abundance of structure in embryo and questions epigenesis

Predates cell theory

Epigenesis Embryonic parts

arise from embryonic tissue. Newly formed organs.

Kasper Friedrich Wolff provides evidence that embryonic organs arise from tissue that has no adult counter part!

Sets the foundation for Germ layer theory.

“Developmental Force” Immanuel Kant and

Johann Friedrich Blumenbach (mid to late 1700s).

Mystical force leads to development of embryos.

This “force” is heritable from germ cells and highly susceptible to change.

Mendel’s work almost a century later…

Developmental force= Genetics?

Using the microscope revolutionizes development Christian Pander,

Heinrich Rathke, and Karl Ernst von Baer.

Identify the three germ layers: Ectoderm Mesoderm Endoderm

The Three Germ Layers

Triploblastic and diploblastic Organisms These organisms use all three germ layers. Some organisms only use two germ layers

(lack mesoderm): Porifera (sponges) Cnidarians (sea anemones, hydra, jellyfish) Ctenophores (comb jellies)

Axes and Symmetry

Christian Pander Work with Chick

Embryos. Supports epigenesis

by showing that tissues worked together to form organs.

Discovers tissue interaction-Induction: No tissue can form

organs alone, must interact with other tissues in order to for organogenesis to occur.

Heinrich Rathke Discovered

pharyngeal arches. In fish, these arches

give rise to gill apparatus

In humans, form jaw, ears, vertebrate skull.

Karl Ernst von Baer “Father” of

Developmental Biology Expanded Pander’s

studies of Chick Embryos.

Identifies notochord- rod of dorsal-most mesoderm tissue.

Notochord divides embryo in right and left sides, instructs ectoderm above it to differentiate into nerve tissue.

von Baer laws Rule 1: The general

features of a large group of animals appear earlier in development than do the specialized features of a smaller group.

All vertebrates have similar structures during development: gill arches, notochord, primitive kidneys.

Very similar shortly after gastrulation.

von Baer’s laws Rule 2: Less General

characters are developed from the more general, until finally the most specialized appear.

All vertebrates have skin, later skin becomes feathers for birds, scales for reptiles, hair and nails for mammals

von Baer’s Laws Rule 3: The embryo of a given species, instead

of passing through the adult stages of lower animals, departs more and more from them.

Visceral clefts of embryonic birds/mammals do not ressemble gill slits of adult fish.

von Baer’s Laws Rule 4: Therefore,

the early embryo of a higher animal is never like a lower animal, but only like its early embryo.

We never pass through a developmental stage similar to adult fish or birds.

Are there really similarities?

The Cell theory and Fate mapping Matthias Schleiden

and Theodor Schwann in 1838 formalize the Cell theory: all living organisms are composed of cells!

Embryologists followed this movement and began tracking cell movement during development!

Two Cell types: Epithelial and Mesenchymal Epithelial Mesenchymal

Morphogenesis Direction and No. of

cell divisions Cell shape changes Cell movement Cell growth Cell death.

Cell Movement and association

Cadherin based adhesion-self association.

Different strength of interaction

Morphogenesis

How do you fate map cells? Observing live

embryos! Edwin G Conklin

Still used as primary fate mapping technique.

Zebra Fish will allow you to do this!

How do you fate map cells? Vital Dye Marking Vital dyes stain the

cells but don’t kill them. Dead cells tell no

tales…unless you study apoptosis.

How do you fate map cells? Radioactive/

Fluorescent labeling Radioactive label:

Transplant of graft from radiolabeled embryo to unlabeled embryo. Silver stain!

Fluorescent labeling: Inject fluorescent dye into cells of embryo.

How do you fate map cells? Genetics! Use cells from

another organism that contain a easily detectable marker!

Darwin: Embryology’s biggest fan! Saw Embryology as

support for his theories. “Community of

embryonic structure reveals community of descent”

This is shown in Homologous structures, such as the forelimb of human arms vs. wings of a bird!

What would be an analogous structure?

Evolutionary change is due to developmental change.. Ontogeny causes

phylogeny? Human fingers

versus Bat wing!