dfs magnetic sector gc-hrms - from toronto 2005 to sao...

DFS Magnetic Sector GC-HRMS

F T t 2005 t Sᾶ P l 2015

1The world leader in serving science

Dr. Dirk Krumwiede, Dr. Heinz MehlmannThermo Fisher Scientific

From Toronto 2005 to Sᾶo Paulo 2015

10 Anniversary DFS GC-HRMS – Dioxin Conf. 2015

2

Our commitment to the DFS Magnetic Sector GC-HRMS

Buy with confidence...

• Letter of commitment to the Thermo Scientific™ DFS™ Magnetic Sector GC-HRMS

• Thermo Fisher Scientific are committed to

3

Thermo Fisher Scientific are committed to DFS production until at least 2023, likely beyond

• Committed to parts, service and application support until at least 2030, likely longer

Our commitment to the DFS Magnetic Sector GC-HRMS

Buy with confidence...

• Letter of commitment to the DFS Magnetic Sector GC-HRMS

• Thermo Fisher Scientific are committed to DFS d ti til t l t 2023 lik l

4

DFS production until at least 2023, likely beyond

• Committed to parts, service and application support until at least 2030, likely longer

Strong commitment to DFS GC-HRMS as TFS flagship for Dioxin analysis

X

Andreas HilkertDFS product manager

Heinz MehlmannDFS application scientist

Frank Theobald (freelance)DFS instructor

Dirk KrumwiedeDFS application scientist

The faces of the DFS Magnetic Sector GC-HRMS: the application team

5

6

Toronto 2005 to Sᾶo Paulo 2015…

From Toronto 2005…

10 Years after…

7

Photo by Keven Dietrich

From Toronto 2005 to Sᾶo Paulo Today…

10 Years after…

8

Photo by Keven Dietrich

9

DFS GC-HRMS, a look back…

…how did it start?

1948: “CH1”, first MS prototype/birth of MAT

- Dr. Ludolf Jenckel(Atlas Werke AG) together with one collegue develops in 1 year the first MS prototype in the cellar of a hospital

11

- After completition he persuades his company to set up a small devision for MS: Atlas MAT

1958: “CH4” – First International Success

- More than 400 installed by 1967

- Applications: e.g.Analysis of coffee aroma (headspace);Perfume mixtures;Cigarette smoke

12

- MAT expands

- Early sixties:GC attached to MS

First GC/MS: CH4 coupled to capillary GC

1972: “MAT 311” (double focusing MS)

13

Max. Resolution ?

1982 – 1986: “8200”

14

1987 – 2005: MAT90 / 95 series

• 1987 - MAT 90• 1992 - MAT 95• 1995 - MAT 95 S• 1998 - MAT 95 XL• 2001 MAT 95 XP

15

• 2001 - MAT 95 XP

Our unique dual GC configuration started with MAT95…

2005: DFS GC-HRMS market introduction, Dioxin conf. Toronto

http://www.dioxin20xx.org/ohc_database_search.htm

16

Jens Griep-RamingProject manager DFSGC-HRMS development

DFS GC-HRMS: from sketch...

17

Original first drawing on a piece of paper by Thermo Fisher Scientific designer

...to reality

18

Note: DFS GC-HRMS as introduced in 2005 with former GC and Autosampler model

DFS GC-HRMS – impressions from R&D time

Construction: “stability test” GC table

GC table can hold 80 Kg!!

19

Electronics: “talking to the boards”

The DFS GC-HRMS key feature? – Toroidal ESA for…

20

…more sensitivity, more ions at same resolution

100 fg 2370 TCDD

DFSGC-HRMS

Same scaling

21

100 fg 2370 TCDD

MAT95

What was new with the DFS GC-HRMS?

• Toroidal ESA for more ions• Instrument shipped in one unit, no assembling of MS onsite• all inside e.g. roughing pumps – only exception: water chiller• moves on wheels• lightest double focusing magnetic sector• State-of-the-art electronics• new robust detector dynode design

22

• new robust detector dynode design• quick exchange ion source• new software, etc., etc.

Almost a benchtop GC-MS, one that stands on the floor……and comes with a built-in bench

The name? Double Focusing Sector “DFS”

2006: DFS GC-HRMS at customer lab in ChinaD:\Xcalibur\...\dk_tcdd22 9/26/2006 11:49:18 AM man. 1 ul 2 fg tcdd (2)new syringe, BACK, DB5 30 m x 0.25 mm (0.1), 120 (2)-15-240(0)-6-270, 0.8 ml/min, splitless 260 C, 1 min, aux 270 C, ists 270, 45 eV, ists 260

RT: 7.61 - 12.47

20

40

60

80

100

ativ

e A

bund

ance

0

20

40

60

80

100

RT: 11.83AA: 83.50

RT: 11.83AA: 114

NL:6.70E1m/z= 319.7970-319.9970 MS ICIS dk_tcdd22


2 fg native 2378-TCDD

1/50 dilution of new, sealed, certified 2378-TCDD

23

8.0 8.5 9.0 9.5 10.0 10.5 11.0 11.5 12.0Time (min)

0

20

40

60

80

100

0

20

40

60

80

100

0

20

Rel

a

RT: 11.83AA: 5584

RT: 11.82AA: 6419



100 fg 13C labeled 2378-TCDD

certified 2378 TCDD specification solution

24

How do we see the DFS GC-HRMS today?

Wh “G ld” St d d?

26

Why “Gold” Standard?

DFS GC-HRMS: Gold Standard for Dioxins

27

DFS GC-HRMS – designed for Dioxin analysis

DFS GC-HRMS: Gold Standard for Dioxins

Proven over 10 years:

o Best Sensitivityo Combined(!) with Robustnesso Long instrument Lifetime

28

DFS GC-HRMS – designed for Dioxin analysis

DFS GC-HRMS robustness – large volume ion source

Foto:Ion source exchange

29

“Robustness by size”

DFS GC-HRMS ion source - designed for combining highest sensitivity with robustness, because sensitivity alone is not enough.

“No, this is not only one single, nicely selected chromatogram”

“Yes, this is an installation spec”

“We demonstrate this specification on every single DFS GC-HRMSduring installation.” 60

65

70

75

80

85

90

95

100

ce

10.83

20 fg 2378-TCDD SN ≥ 200:1 (PtP, 4)

DFS GC-HRMS – Gold Standard Dioxin installationspec

30

• A Dioxin spec, 2378-TCDD • in front of the customer• using a sealed, certified standard

“The best Dioxin spec existing: DFS GC-HRMS - Gold standard Dioxin spec”

7.5 8.0 8.5 9.0 9.5 10.0 10.5 11.0 11.5Time (min)

0

5

10

15

20

25

30

35

40

45

50

55

Rel

ativ

e A

bund

anc

10.91 11.0910.299.09 11.609.268.81 11.268.78 10.04 10.539.749.338.478.137.68 7.92 9.638.407.59

Application NormThermo Scientific

DFSGC-HRMS

Food EU Regulation EU Regulatory Feed Control (at ML) ApprovedFood EU Methods EN 16215 ApprovedFood EURL Recommendation Background food studies (<1/5th EU ML) RecommendedClinical EURL Recommendation Human studies at trace levels RecommendedEnvironmental EU Method EN 1948 ApprovedEnvironmental US Method US EPA 1613 B for strict EPA compliance ApprovedEnvironmental US Method US EPA Method 23 ApprovedEnvironmental US Method US EPA Method 8290 Approved

DFS GC-HRMS – worldwide official method compliance

31

ppEnvironmental Japan Method JIS K0311 ApprovedEnvironmental Japan Method JIS K0312 Approved

DFS GC-HRMS offers worldwide compliance with official Dioxin methods or regulations like EPA 1613 and all others….

…also PCBs, PBDEs (EPA 1668, 1614,…)

RT: 23.82 - 27.83

0

50

100

Rel

ativ

e A

bund

ance

0

50

100

0

50

100

0

50

100

RT: 25.22MA: 375

24.0823.90 25.52 27.40 27.7527.2324.19 24.36 27.0726.2925.3724.54 25.63 26.6625.83 26.9226.4626.0324.74

RT: 25.22MA: 432

24.13 24.52 27.6424.95 27.2926.7726.22 27.0526.0325.54 25.85 26.55 27.5126.9026.3524.67

RT: 25.20AA: 172156

RT: 25.20AA: 218158

NL: 1.13E2m/z= 303.8868-303.9172 MS PCDDF_sample_219448-703_2ul_01


NL: 4.01E4m/z= 315.9262-315.9578 MS ICIS PCDDF_sample_219448-703_2ul_01


DFS GC-HRMS: human serum sample – lowest levels

ca. 3 fg TCDF

32

24.0 24.2 24.4 24.6 24.8 25.0 25.2 25.4 25.6 25.8 26.0 26.2 26.4 26.6 26.8 27.0 27.2 27.4 27.6 27.8Time (min)

0

50

100

0

50

100

0

50

100

0

50

100

0R

RT: 25.81MA: 165

27.54

27.6724.17 27.1625.26 27.4024.60 26.1825.57 26.9926.6624.87 25.9624.39 26.46

RT: 25.85MA: 202

26.73 27.2527.7725.57 27.5424.61 27.1224.32 26.6026.2224.02 25.26 25.4624.96 27.0125.70 26.4626.05

RT: 25.22AA: 153519

RT: 25.79AA: 116608

RT: 25.22AA: 198314 RT: 25.79

AA: 159458





ca. 2 fg TCDD

For research purposes only. Not for use in diagnostic procedures.

Why has the DFS GC-HRMS been so successful?

finally we know it

33

…finally we know it…

POPs want to be analyzed by DFSGC-HRMS!!

.. have a closer look into the World of Dioxins..

34

• Market leader in Dioxin analysis • DFS GC-HRMS is designed for

Dioxin analysis• worldwide use in Dioxin expert labs

• Worldwide method compliance• Dioxins, PCBs, PBDEs• EPA 1613 1668 1614 & others

DFS GC-HRMS benefits at one glance

• Specification• Gold standard Dioxin spec, best Dioxin

spec on the market, best sensitivity• Dioxin spec shown at every installation

• Proven, safe bet• DFS GC-HRMS is the “gold standard”

and benchmark for Dioxins

35

• EPA 1613,1668,1614 & others• Use PFK, lockmass trace etc.

• References (since 2005)• installed in hundreds of Dioxin labs• Proven in >> 100.000 injections• round robin tests since 2005 • scientific papers

and benchmark for Dioxins• Be prepared for all Dioxin applications• Unique Dual GC configuration

• Superior performance• DFS GC-HRMS = ultimate analytical

performance: sensitivity, linearity etc. combined with robustness & long instrument lifetime

Thank Thank you for you for your attention your attention

36

dfs magnetic sector gc-hrms - from toronto 2005 to sao...

Documents