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DIAGNOSTIC INSTRUMENTS, INC 6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • www.diaginc.com Image credits: Paul Danielson, Albany Research Center, OR Dr. Michael W. Davidson, Florida State University Dr. Rob Weaver, McCrone Institute Joseph J. Buchino, M.D. Director of Pathology at Virginia Hospital Dr. Catherine Carr, MBL,Woods Hole, MA

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Page 1: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR

DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs StreetSterling Heights, MI 48314

586.731.6000 • www.diaginc.com

Image credits:

Paul Danielson, Albany Research Center, OR

D r. Michael W. Davidson, Florida State University

D r. Rob We a v e r, McCrone Institute

Joseph J. Buchino, M.D. Director of Pathology at Virginia Hospital

D r. Catherine Carr, MBL,Woods Hole, MA

Page 2: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR
Page 3: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR

• Reflected Light• Metallurg y• Semiconductor• Metro l o g y• Fore n s i c s• Macro - p h o t o g r a p h y• Machine Vi s i o n

M e t a l l u rgy

High Res I n s p e c t i o n

H&E slide

D a r k f i e l d

Phase

DIC Triple stain

Karyotype

• Phase• DIC• Hoff m a n

• Brightfield• Darkfield• Pathology• Histology• Gross Anatomy

• Fixed Cell Fluore s c e n c e• Karyotyping• FISH

Best fit for application

Suitable for application

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Page 4: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR

YFP

GFP/Phase

C h e m i - g e l

L u c i f e r a s eTwo Photon

S M F

• Fluorescent Proteins • GFP• YFP

• Chemiluminescence• Bioluminescence• Gene Expression Pro f i l e s• Whole Org a n i s m

F l u o re s c e n c e

• SMF• Two Photon

Calcium Ratio

F R E T Quantum DotC o n f o c a l

• FRET • Ion Imaging • Fura• Flow 4

• FRAP • TIRF• Particle Tr a c k i n g• 4D- 6D Imaging• Quantum Dot

• Real-time Confocal

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Page 5: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR

I N S I G H T ™ cameras provide a feature packed imaging system for most documentation

applications. When you buy an I n s i g h t camera, you benefit from engineering expertise

gained from Diagnostic Instruments’ re s e a rch and development into high-end quantitativecamera designs. The result is low noise, high speed image captures that produce stunningly

clean views of your specimen. Some of the true strengths of the I n s i g h t camera systemcomes from the SPOT software that not only makes it easy to control the sophisticated

h a rd w a re, but takes you well beyond image capture. Powerful, yet intuitive software

functions provide many microscopists with all the tools they will ever need.I n s i g h t gets your work done!

I N S I G H T | unsurpassed value

Key Features:• Cost effective package/solutions

• High resolution replacement for video capture

• Real-time viewing

• Plug and play computer interface

• Powerful, easy to use software

• Color and monochrome models

F e a t u re s : B e n e f i t :

40 MHz live mode (dual channel 20 Mhz) Real-time viewing of specimens

14 bit x 20 Mhz capture High speed, low noise capture

1-20x programmable gain Facilitates low light imaging

2 & 4 Mpixel CCD sensors High resolution imaging1” & 4/3” Format CCD sensors Get full field-of-view without expensive couplers

High quantum efficiency CCD I n c reases sensitivity for faster capture s

1394a Fire W i re™ interface Fast, easy to install computer interface

SPOT software Easy to use powerful imaging enviro n m e n t

A p p l i c a t i o n D e s c r i p t i o n M o d e l S u i t a b i l i t y

P a t h o l o g y, Highest resolution, highest field-of-view IN 1430 E x c e l l e n t

M e t a l l u rg y, Highest resolution, standard field-of-view IN 1830 E x c e l l e n tColor Brightfield S t a n d a rd resolution, highest field-of-view IN 1420 G o o d

S t a n d a rd resolution, standard field-of-view IN 1820 G o o d

Color Brightfield & S t a n d a rd resolution, highest field-of-view IN 1420 G o o dBright Fluore s c e n c e S t a n d a rd resolution, standard field-of-view IN 1820 G o o d

Visible Spectrum Highest resolution, highest field-of-view IN 1410 E x c e l l e n t

M o n o c h rome Brightfield Highest resolution, standard field-of-view IN 1810 E x c e l l e n t

B road Spectrum Highest resolution, highest field-of-view IN 1400 E x c e l l e n t

M o n o c h rome Brightfield Highest resolution, standard field-of-view IN 1800 E x c e l l e n t

DIC/Phase plus S t a n d a rd resolution, highest field-of-view IN 1420 G o o dFixed Cell Fluore s c e n c e S t a n d a rd resolution, standard field-of-view IN 1820 G o o d

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Page 6: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR

FLEX™ Cameras combine acquisition flexibility with category leading specifications to set the new benchmark for high-resolution documentation applications. With Flex shifting

sensor technology, you can select the resolution dependent on your needs: single-shot 4 MP

capture for live/moving specimens, 4-shot 4 MP full color sampling for high magnification-

fixed specimens, 16-shot 16 MP for low magnification-fixed specimens, or a 64 MP color(using image double) mosaic image for smooth large format publication.

Flex monochrome goes even further, providing 9-shot, 36 MP resolution and 16-shot, 64 MP

true resolution without image doubling. With the higher QE of the monochrome CCD and

14-bit data capture, this is a perfect imaging camera for high resolution industrial inspectionapplications.

Flex cameras are an excellent solution for capturing images of both brightfield and

fluorescence specimens. Making all this power and flexibility easy is SPOT Software thatcombines real-time viewing and image capture with essential post processing, storage and

simple automation. Flex lets you do just what you want to do.

do what you want to do | F L E X

Key Features:• Flexible resolution from high to ultra-high resolution

• Phenomenal color from co-site sampling

• Wide field-of-view

• Highly cooled

• Real-time viewing

• Plug-and-play computer interface

• Powerful, easy to use software

• Color model

• Extreme resolution monochrome model

F e a t u re s : B e n e f i t :

4 Mpixel CCD sensors High resolution single-shot imaging Flex™ shifting sensor technology Ultra-high resolution multi-shot imaging

Color co-site sampling Truest high resolution color

40 MHz live mode (dual channel 20 Mhz) Real-time viewing of specimens

14 bit x 20 Mhz capture High speed, low noise captureHigh quantum efficiency CCD I n c reases sensitivity for faster capture s

- 20°C diff e rential cooling Facilitates extended exposure for low light imaging

1-20x programmable gain Facilitates low light imaging

4/3” format CCD sensors Full field-of-view without expensive couplers1394a Fire W i re™ interface Fast, easy to install computer interface

SPOT Software Easy to use, powerful imaging enviro n m e n t

A p p l i c a t i o n D e s c r i p t i o n M o d e l S u i t a b i l i t y

P a t h o l o g y, Ultra-high resolution, highest field-of-view FX 1520 B e s t

Color Brightfield& Fluore s c e n c e

DIC/Phase plus Ultra-high resolution, highest field-of-view FX 1520 B e s t

Fixed Cell Fluore s c e n c e

M e t a l l u rg y FX 1520 B e s tFX 1500 G o o d

I n s p e c t i o n E x t reme resolution, highest field-of-view FX 1500 B e s t

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The SPOT RT 3™ digital camera system is the newest version from the immensely popular SPOT

RT™ line of cameras, combining improved performance with F i re W i re® connectivity.

With an effective diff e rential cooling of –43°C, and improved CCD sensor design, dark current hasbeen suppressed to 0.003 e/p/s. This is a 20x improvement making the RT 3 an excellent

performer for longer exposure fluorescent applications.

Image acquisition rates have been tripled, while reducing read noise by over 25%, providing even

higher dynamic range when combined with the deep pixel wells of its Kodak KAI 2020 CCD.

The RT 3 camera is off e red in monochrome, color mosaic, and user selectable color/monochro m eslider models, making this a great performer for both your documentation and quantitative re s e a rc h

n e e d s .

RT 3 | your new favoriteKey Features:• Tremendous value

• Low noise

• Highly cooled, very low dark current

• FireWire connectivity

• Real-time viewing

• Powerful software support

• Smaller size

• Color, monochrome and slider (combined color and monochrome) models

F e a t u re s : B e n e f i t :

2 Mpixel CCD sensors High resolution single shot imaging

16 e- read noise P recise readings for analytical applications

- 4 3°C diff e rential cooling, dark current 0.003 e/p/s Major dark noise re d u c t i o n

14 bit x 20 Mhz capture Over 16,000 potential brightness levels

I m p roved high quantum efficiency CCD I n c reases sensitivity for faster capture s

High speed live mode Real-time viewing of specimens

P rogrammable gain Facilitates low light imaging

SPOT Software Easy to use, powerful imaging enviro n m e n t

L a rge 3rd party driver selection Choice of application software

F i re w i re (IEEE 1394a) Plug and play high speed connectivity

A p p l i c a t i o n D e s c r i p t i o n M o d e l S u i t a b i l i t y

R e s e a rch pathology RT 2540 E x c e l l e n t

DIC plus RT 2500 E x c e l l e n t

Fixed Cell Fluore s c e n c e

F l u o rescence pro t e i n s Cooled high sensitivity RT 2500 Very GoodG F P, YFP

C o re lab Multiple application capability: Brightfield, RT 2540 E x c e l l e n t

Fixed Cell Fluorescence, DIC, Phase

Fixed Cell Fluore s c e n c e RT 2540 E x c e l l e n t

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Page 8: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR

PURSUIT™ 4MP cameras are solid, low noise cameras that provide a large field of view without need of

reduction optics. Low noise read out and regulated cooling facilitates critical quantitative applications.

X P L O R E R™ 4MP cameras bring to bear top performance specifications on all fronts, including superior

regulated cooling, making it the obvious choice for labs that need to perform a wide variety of critical quantitative applications including long exposure, low light imaging.

C o n t rol of these cameras can be accomplished by SPOT S o f t w a re for simple to moderately complex imaging

tasks, or by using a number of sophisticated 3rd party applications that specialize in complex control,

automation, and analysis of whole experiments. Both cameras are available in S l i d e r v e r s i o n s .

P u r s u i t and X p l o rer 4MP cameras let you see the whole picture .

see the world through a larger window | PURSUIT/XPLORER 4MP Key Features:• Large field-of-view

• High resolution

• Low noise readout with optimized streaming mode

• Software selectable readout speed

• High dynamic range

• Deep, regulated cooling

• Real-time viewing

• Powerful software support

• Monochrome and slider (combined color and monochrome) models

F e a t u re s : B e n e f i t :

Regulated cooling to -42º C (Xplorer), -10º C (Pursuit) Repeatable thermal frames and major dark noise

reduction for long exposure applications.

4 Mpixel CCD sensors High resolution single-shot imaging

21.4 mm CCD diagonal L a rge field-of-view10 e- read noise P recise readings for analytical applications

14 bit capture 16,384 brightness levels

10 & 20 MHz readout S o f t w a re selectable readout speed

30,000 e- well depth (1x1 binning) High dynamic range60,000 e- well depth (2x2 binning) Extended dynamic range at lower re s o l u t i o n

High quantum efficiency CCD I n c reases sensitivity for faster capture s

High speed live mode Real-time viewing of specimens

1-20x programmable gain Facilitates low light imagingSPOT Software Easy to use, powerful imaging enviro n m e n t

L a rge 3rd party driver selection Choice of application software

A p p l i c a t i o n D e s c r i p t i o n M o d e l S u i t a b i l i t y

DIC & Fluore s c e n c e PR 1600 E x c e l l e n t

H&E and PR 1640 E x c e l l e n tFixed Cell Fluorescence

Bioluminescence & Deep cooling, low noise, 50% QE XP 1700 E x c e l l e n tC h e m i l u m i n e s c e n c e

C o re lab Multiple application capability: color and XP 1740 B e s tm o n o c h rome imaging from brightfield to PR 1640 E x c e l l e n tlow light applications

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Page 9: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR

The S P O T line of Boost EMCCD camera systems utilizes Electron Multiplication technology to deliver supreme performance for extremely low light microscopy applications.

E l e c t ron multiplication gain of up to 1000x offers the capability to extract extremely faint signals without the need for an image intensifier.

The B o o s t camera is off e red in three base models of CCD formats and includes front illuminated and extremely sensitive back illuminated (>92% QE) versions. Flexibility is a

hallmark of this camera line with selectable read speeds, adjustable vertical clocking,adjustable True Linear gain multiples, electron multiplying and non-EMCCD read options.

Cooling options allow this camera system to achieve –100º C cooling while vacuum-sealingtechnology insures that cooling performance will be maintained for years to come.

Data can be acquired at up to 16 bits with less than one percent non-linearity, assuring data integrity for even the most demanding imaging applications. Using electron multiplication

t e c h n o l o g y, phenomenal frame rates ( over 5,000 subframes per second )can be achievedwith effectively negligible read noise.

B O O S T | redefining the detection limit

Key Features:

• Electron Multiplication Gain Technology

• Extreme, regulated cooling to -100º C

• Resolution up to one megapixel

• Selectable readout speed and non-EMCCD mode

• Full frame acquisition over 500 f/s

• SPOT software interface

• High level 3rd party software support

B T 1 9 0 0 B T 2 0 0 0 B T 2 1 0 0

S t a n d a rd -85º C -85º C -85º C

With supplemental cooling -90º C -90º C -90º C

With optimum cooling -100º C -100º C -100º C

Active pixel capacity 200,000 e- 220,000 e- 40,000 e-Gain register pixel capacity 800,000 e- 800,000 e- 80,000 e-

Full frames 500 f/s 35 f/s 31.5 f/s

S u b f r a m i n g 5,000 f/s 526 f/s 446 f/s

Selectable in MHz 10, 5, 3, 1 10, 5, 3, 1 35, 27, 13

KE Y PE R F O R M A N C E B Y M O D E L

Deep cooling

Significant well-depth

High-speed capture

Multiple speed re a d o u t

A p p l i c a t i o n S u i t a b i l i t y

S M F, Two Photon B e s t

Real-time confocal, TIRF, Particle tracking Best (also consider Xplorer XS)

F l u o rescence, FISH, GFP Good (Xplorer is best)

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Page 10: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR

Many new techniques for studying live organisms result in observations at

low light levels. These techniques have been constrained by the frame rates, resolution, sensitivity, and electronic noise levels of the cameras used to capture

the images. Higher resolution and shorter exposures resulting from higher framerates reduce the number of signal photons available for capture within each

pixel. Concurre n t l y, higher clocking speeds used for higher frame rates incre a s ethe read noise contribution.

The result is a lower signal-to-noise ratio, producing images that appear fuzzy or

below the limit of detection. What these applications need is some way to“Boost” the signal-to-noise ratio!

EMCCD Technology involves the

addition of an “ E l e c t ron Multiplication”register to a standard C C D s t r u c t u re .

This register produces a multiplication of the signal electrons through a

phenomena called “Impact Ionization.” The novel and useful aspect of this

phenomena is that signal gain is amplified before the read noise

component is added to the signal. Since this gain can be controlled, the

signal can easily be BOOSTED wellbeyond any increased read noise during

high speed clocking of the CCD. Thisopens the way for acquiring higher framerates through shorter exposures even in

e x t remely low light level applications.

T h a t ’s why most Boost cameras are designed using extremely e fficient back-thinned CCD sensors.

Once the detection limit reaches one to two photons, any individualnoise electron will deteriorate the signal-to-noise ratio. A critical

remaining source of noise is thermally generated dark current. It isparticularly troubling since it is generated concurrently with the signal

e l e c t rons and when it occurs, it is indistinguishable from a signal e l e c t ron. Boost cameras eliminate this source with -70º C cooling

s t a n d a rd and optional -100º C cooling for the most demanding ofa p p l i c a t i o n s .

EMCCD Technology providesthe answer. . .

Every electron counts…

M o re cooling, less noise

Single line S/R at -95º CSingle line S/R at -50º CSingle line S/R at -30º C

technology | EMCCD

Boost - EMCCD, a new solutionfor special microscopy needs.

Back-thinned CCD - BT2000 (at -20º C)

Example of the magnitude of reduced thermal noise provided by Boost cooling. 9

Page 11: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR

The XS series of Pursuit and Xplorer digital cameras aredesigned for applications where speed, sensitivity and dataintegrity are the primary imaging re q u i re m e n t s .

These cameras have been designed with extremely quiet electronics to

s u p p ress read noise and also provide regulated cooling to virtually eliminateany effects of dark current ( X p l o re r- X S) .

The XS series is perfect for live cell imaging and fast occurring events, as well as providing the flexibility to deliver incredible results for low light, long exposure fluorescence work.

These cameras are off e red in monochrome and slider models.

PURSUIT XS | satisfy your need for speed

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Page 12: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR

Key Features of the XS Series:

• Low noise, high-speed readout with optimized streaming mode

• High QE CCD >60% – Sony ICX-285

• High dynamic range

• Software selectable readout speed

• Excellent resolution

• Regulated cooling with deep cooling (Xplorer)

• Real-time viewing

• Powerful software support-SPOT and 3rd party

• Monochrome and slider (combined color and monochrome) models

fast, cool, quiet - the perfect combination | XPLORER XS

X S SE R I E S

Full frames Over 11 frames per second

S u b f r a m i n gw / b i n n i n g Over 130 frames per second

High QE CCD– Sony ICX-285 Over 60% QE

Read noise suppre s s i o n 4.5 e- @ 10 MHz5.5 e- @ 20 MHz

Thermal noise suppre s s i o n - 1 0º C regulated Pursuit

- 4 2º C regulated Xplore r

A p p l i c a t i o n S u i t a b i l i t y

Ion imaging Excellent / Best

F R E T, FRAP Excellent / Best

Real-time confocal E x c e l l e n t

C o re lab flexibility E x c e l l e n t

KE Y PE R F O R M A N C E

High-speed capture

S e n s i t i v i t y

Low noise

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Page 13: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR

...SPOT Hardware MakesThe Difference!

See what you have been missing...

S P O T | hardware

Advanced technologies to meet your needsS P O T cameras are designed from the start for use in scientific

imaging applications. This design philosophy is essential forhigh-end quantitative imaging applications and the benefits can

also be seen in the beautifully clean images throughout the entireS P O T camera line.

High QE scientific image sensors, the first step to sensitivityS P O T cameras use the latest generation of high quantum

e fficiency sensors from Kodak and Sony. These interline sensors can achieve over 60% peak QE, have large dynamic

range, and provide extremely low noise readouts. Thesei m p rovements allow the current generation of interline sensors to

rival and surpass full frame sensors for sensitivity, while pro v i d i n g

the reliability and speed of electronic shuttering.

Low noise circuitry, the key to preserving sensitivityS P O T cameras preserve pixel values with sophisticated 4th

generation analog-to-digital circuit design resulting in low noise,high speed, 12 & 14 bit digitization. Computer connection is via

1394a Fire W i re™ or Universal PCI slots, providing ease of connectivity and speed.

Advanced Peltier cooling provideslow light performanceS P O T cooled cameras utilize advanced, low maintenance

Peltier cooling systems to suppress dark current and bright point noise. Unregulated cooling models primarily provide noise

reduction while regulated models can take advantage of thermal correction frames to provide fully corrected

image data.

KAI4021

KAI2020

ICX285

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Page 14: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR

Application Driven Solutions:Choose the model that’s right for you.

hardware | S P O T

Diagnostic Instruments designs and manufactures a variety of diff e re n t

camera models precisely because there are many diff e rent applications with

d i ff e rent needs. Don't settle for the one camera fits all solutions that some

m a n u f a c t u rers present you, get the camera that is right for your application.

Monochrome ModelsN o n - i n f r a red filtered models are very useful for scientific imaging of single

wavelength light into the near infrared of both fixed and moving specimens.The addition of an IR blocking filter facilitates a broad spectrum, visible

light observation of both fixed specimen and moving specimen samples.

Color Mosaic Te c h n o l o g yThis technology coats individual pixels on the sensor with either a re d ,g reen or blue filter. When the CCD sensor is read, the missing two colors

for each pixel location are calculated from the surrounding pixels that contain

the missing color. The advantages of this technology includes its capture

of the color data in one shot, its low price, its capability to image livespecimens and its high sensitivity.

3-Shot Color Te c h n o l o g yThis technology adds a re d - g reen-blue color-changing filter in front of the

CCD. Three consecutive images are captured, one in each of red, green andblue color states- measuring every color value at every pixel. The advantage

of this technology is the full resolution of the CCD is maintained for each

color and the color-changing filter can be shifted out of the light path to

allow quantitative monochrome imaging in the same camera. This colorc a p t u re has comparable resolution to a color mosaic camera with four times

as many pixels. The primary limitation for this technology is that it has

limited use with moving specimen images on phase contrast set-ups.

FLEX™ Shifting Pixel Color Te c h n o l o g yThis technology starts out with a color mosaic sensor and mounts it on a

piezoelectric x-y carriage. The shifting pixel camera can take a single shot

and provide color mosaic resolution that has all the pros and cons of that

t e c h n o l o g y. In a second mode, the camera takes four consecutive images asthe piezoelectric carriage moves the whole sensor in one-pixel increments in a

2 x 2 matrix so that each pixel location in the image will have each of the

sensors colors shifted through it. This mode provides full color sampling

much like 3-shot color sampling. A third mode moves the sensor 16 half pixelmoves in a 4 x 4 matrix providing full color sampling at 4 times the CCD

resolution. Flex technology provides standard color mosaic resolution for

moving specimen images, as well as high & ultra high resolution modes for

fixed specimen images.

COLOR MOSAIC TECHNOLOGY

3-SHOT TECHNOLOGY

4-SHOT TECHNOLOGY

CAPTURE 1

CAPTURE 2

CAPTURE 3

SHIFT 2

SHIFT 3

SHIFT 1

SHIFT 1

SHIFT 2

SHIFT 3

SHIFT 1

SHIFT 2

SHIFT 3

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Page 15: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR

Use color imaging modewhen full color brightfield

i m a g i n g is required.

CO L O R SE T T I N G

CA P T U R E 1

CA P T U R E 2

CA P T U R E 3

BR I G H T F I E L D SP E C I M E N

CA P T U R E FI N A L IM A G E

FL U O R E S C E N C E SP E C I M E N

AD D PA L L E TCO L O R

CO M B I N ECA P T U R E S

FI N A L IM A G E

MO N O C H R O M E SE T T I N G

When the slide lever is pushed in, a clear glass compensator“slides” between the sample and the CCD. The image is then projected and captured in monochrome.

Pulling the slide lever moves the color filter into place. The projected image is filtered for each primary color, red, green and blue. The computer combines all threeimages to provide the final full color, full resolution image.

Use monochrome when youwant maximum sensitivityor when monochromea c q u i s i t i o n is appropriate(fluorescent imaging, live cell activities).

• When would you use it?

S P O T S L I D E R C A M E R A | true resolution color / high sensitivity monochrome

The “magic” is in our control of the filter, allowing it to filter for each of the necessary colors through a single filter assembly. The image is then reconstructed by your computer, giving you the highest possible “true” resolution color images.

• How does it work?To begin with, each slider camera acquires its images utilizing a highly efficient monochrome CCD. The slider’s design and use are really quite simple.

• What is it?SPOT Slider style cameras offer users the flexibility of capturing images in eithercolor or monochrome without the need to purchase two individual cameras.

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3RD PA RTY SOFTWARE SUPPORT

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Page 17: DIAGNOSTIC INSTRUMENTS, INC...DIAGNOSTIC INSTRUMENTS, INC6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • Image credits: Paul Danielson, Albany Research Center, OR

S P O T | software

Set up quickly…Start with a pre d e f i n e dsetup, then optimize it for

your particular technique.Name it, save it and thenrecall it the next time you

use that technique. SPOT Software gets youup and running quickly

and then learns your p re f e rences.

Make a point…Highlight important features with text, lines,a r rows, and shapes. SPOT Software helps youillustrate your images.

You control the result…SPOT Cameras combine the ease of a video-like focus,

framing and scanning environment with the high re s o l u t i o nimage capture you expect from digital imaging cameras. WithSPOT Cameras you get your work done quickly by interacting

with your image in real-time and seamlessly capturing highresolution images.

Need to quantify a feature?Just add a calibration mark for re f e rence or measure lengths, perimeters, areas and angles. SPOT Software measures up!

W Y S I W Y G . . .SPOT Software ’s What-Yo u - S e e - I s - W h a t - You-Get Live Image window

p reviews your captured image so you can ensure fine focus and optimal exposure quickly and easily.

Looking for time-lapse or high speed image sequencec a p t u r e ?SPOT Cameras provide an easy to use sequential image captureinterface. Play back your sequences

with the SPOT interactive viewer orsave them in AVI file format ands h a re them with your colleagues

over the Intern e t .

Repetitive tasks made easy…Automate your imaging set-up with MACRO scripting. SPOT MACRO Scripting provides a powerful, menu driven

p rogramming interface for camera, peripheral device and function automation.

Designed for the microscopist, SPOT Software is full featured yet easy to use.

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software | S P O T

Need higher level functions or automation? SPOT Cameras are fully supported byt h i rd party software developers making nearly any imaging task conquerable.

Make it even better…Enhance your images with the extensive

and readily available set of tools in theSPOT Software. SPOT Software supportsmultiple color spaces and multiple bit

depths to allow you maximum control ofyour imaging enviro n m e n t .

Getting the word out… Reporting your results toyour colleagues? Cre a t ea standard pro f e s s i o n a l

p resentation with SPOTS o f t w a re ’s report generator.Reports can be made fro m

open images or from database images and text.

Easy as Drag and Drop…Don't struggle to lay out your images for printing. With SPOT

S o f t w a re just select your images, select a standard layout andprint. Custom layouts are no problem either. Just use yourpointer to interactively position and size your images on your

page. With SPOT, it’s that easy!

Information at your fingertips …Know where your imagesa re when you need them. By

setting up the SPOT imagedatabase, you can catalogyour images with key searc h

fields. Then when you needyour images, set a searc hand retrieve just the images

you want.

Peripheral Device Control?C o n t rol your hard w a re directly f rom the SPOT macro language.The interface includes command

line creation, editing, as well as command library import and export.

Power at your fingertips!Speed your workflow by keeping your most often used

menu functions and macros on the Floating Ta s k b a r, andthen let each user design their own. Simple to set up, a pleasure to use! SPOT software is available

for both Windows andMAC operating systems.

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Be Great… …Show Off Your Mind!

Diagnostic Instruments' 39 years of experience is focused

on producing scientific camera systems that enable its users

to excel. SPOT™ imaging systems integrate sophisticatedoptics, hard w a re and software, to produce an enviro n m e n t

optimized for your specific application. SPOT™ cameras letyou be great and show off your mind!

Many routine digital imaging applications can be handledby the SPOT™ software included with your camera. Now

digital imaging is as easy as picking an image setup, viewing

the specimen in real-time right on your computer, thencapturing a vibrant, high-resolution color or monochro m e

image. Once captured, enhancements may be needed. A few clicks later and your enhancements are done. Next

annotate your image to communicate your thoughts.

Finished with the image, store it to the database with a fewnotes. Months later, open the database and retrieve this

image as well as similar images. Now add calibration marksand measure critical features. Then arrange the images of

i n t e rest on a single sheet and print them out. Need a more

complete presentation? Drop the images into the report g e n e r a t o r, add your analysis and get a hard copy for

p resentation. This is just the start. Repetitive tasks can be

easily automated with SPOT Software ’s macro scriptingcapabilities. These macros and common imaging tools can

be kept near at hand with the floating taskbar. With SPOTCameras the focus is on your work and getting it done, not

on managing the camera.

If your application calls for a higher level of automation or

analysis, be assured that SPOT cameras are supported byone of the widest selections of third party software

applications and hard w a re peripherals.

D I A G N O S T I CINSTRUMENTS | unsurpassed excellence

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not an afterthought | C U S T O M E RS U P P O RT

Not an afterthoughtAlthough SPOT Cameras have been designed for ease of

use, there are times you will need help. In these situations...

Follow the leader. . .As an established member of the microscopy community,Diagnostic Instruments has selected leading imaging

specialists around the world to provide our local

re p resentation. These specialists will handle such thingsas on-site evaluation of your imaging needs, demonstrations

of the SPOT camera product and the integration of SPOT

camera systems with appropriate optical couplers, computers, third party software and hard w a re .

Keep it in shape...Diagnostic Instruments provides SPOT Cameras with

• 1 year software maintenance agre e m e n t

• 2 year hard w a re warranty

To continue coverage after the standard warranty expire s ,please inquire about our extended warranty and software

maintenance pro g r a m s .

Get a helping hand...Diagnostic Instruments’ technical staff is readily available

to help you through any problems you may encounter. If

your problem is particularly difficult, we can consult withour hard w a re, software, optical and application engineers for

g reater insight. At Diagnostic Instruments, the resolution of

your problem is our ultimate goal.

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