different stimuli on tailfan
TRANSCRIPT
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Different Stimuli on TailfanAnam Syed, Alyssa Huenniger, Pratiksha Malbari
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Materials (Salt: NaCl)
--NaCl solutions
--Syringe with wiring to help direct control and test stimuli
--Crayfish tanks (6)
--Crayfish (6)
--Water (filled halfway)
--Control (Tank Water)
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Methods (Salt: NaCl)
--Place crayfish in fresh tank of water
--Begin testing crayfish for reaction
--Insert 3 ml of Tank Water into syringe for control test
--Spray different areas of tail and tailfan/ gills on crayfish; note reaction
--Insert 3 ml NaCl solution (1%, 2%, 3% conc.) into wire syringe
--Spray the same areas of tail and tailfan/ gills; note reaction
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Materials (Salt: CaCl2)
--CaCl2 solutions
--Tank Water Control
--Syringe with wiring to help control and direct stimulus
--Crayfish tanks (6)
--Crayfish (6)
--Water (Tanks filled halfway)
![Page 5: Different Stimuli on Tailfan](https://reader035.vdocuments.net/reader035/viewer/2022062903/58cff49c1a28abfc0a8b4a07/html5/thumbnails/5.jpg)
Methods (Salt: CaCl2)
--Place crayfish in fresh tank of water
--Begin testing crayfish for reaction
--Insert 3 ml of Tank Water into syringe for control test
--Spray different areas of tail and tailfan/ gills on crayfish; note reaction
--Insert 3 ml of CaCl2 solution (0.3%, 0.6%, and 1.2% conc.) into wire syringe
--Spray same areas of tail and tailfan/gills; note reaction
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Materials (Temperature)
--Wire syringe (3) filled with tank water at various temperatures
--3 temperatures used: 12 , 22 , 32℃ ℃ ℃
--Control Tank Water (22 )℃
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Methods (Temperature)
--Place crayfish in fresh tank of water
--Begin testing crayfish for reaction
--Insert 3 ml of Tank Water into syringe for control test
--Spray different areas of tail and tailfan/ gills on crayfish; note reaction
--Insert 3 ml of different temperature water (12 degrees Celsius, 22 degrees Celsius and 32 degrees Celsius) into wire syringe
--Spray same areas of tail and tailfan/gills; note reaction
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Materials (Mink Urine)
--Mink Urine Solution (1% conc.)
--Tank Water Control
--Syringe with wiring to help control and direct stimulus
--Crayfish tanks (6)
--Crayfish (6)
--Water (Tanks filled halfway)
![Page 9: Different Stimuli on Tailfan](https://reader035.vdocuments.net/reader035/viewer/2022062903/58cff49c1a28abfc0a8b4a07/html5/thumbnails/9.jpg)
Methods (Mink Urine)
--Place crayfish in fresh tank of water
--Begin testing crayfish for reaction
--Insert 3 ml of Tank Water into syringe for control test
--Spray different areas of tail and tailfan/ gills on crayfish; note reaction
--Insert 3 ml of 1% concentrated Mink Urine into wire syringe
--Spray same areas of tail and tailfan/gills; note reaction
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Materials (Allyl Isothiocyanate)
--Allyl Isothiocyanate (0.1% conc.)
--Tank Water Control
--Syringe with wiring to help control and direct stimulus
--Crayfish tanks (6)
--Crayfish (6)
--Water (Tanks filled halfway)
![Page 11: Different Stimuli on Tailfan](https://reader035.vdocuments.net/reader035/viewer/2022062903/58cff49c1a28abfc0a8b4a07/html5/thumbnails/11.jpg)
Methods (Allyl Isothiocyanate)
-Place crayfish in fresh tank of water
--Begin testing crayfish for reaction
--Insert 3 ml of Tank Water into syringe for control test
--Spray different areas of tail and tailfan/ gills on crayfish; note reaction
--Insert 3 ml of 0.1% concentration into wire syringe
--Spray same areas of tail and tailfan/gills; note reaction
--Spray long and short antennae and antennules; note reaction
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Results:NaCl
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Results:CaCl2
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Results:Temperature
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Results: Mink Urine
![Page 16: Different Stimuli on Tailfan](https://reader035.vdocuments.net/reader035/viewer/2022062903/58cff49c1a28abfc0a8b4a07/html5/thumbnails/16.jpg)
Results: Allyl Isothiocyanate
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References
1. Bell, J. C. "The Reactions of Crayfish to Chemical Stimuli." Journal of Comparative Neurology & Psychology (1906).
2. Lung, Megan E., et al. "Effect of increasing salinity levels on the foraging behavior of Orconectes virilis in a controlled environment." (2012).
3. Spoor, W. A. "Loss and gain of heat-tolerance by the crayfish." The Biological Bulletin 108.1 (1955): 77-87.
4. Taylor, Robert C. "Thermal preference and temporal distribution in three crayfish species." Comparative Biochemistry and Physiology Part A: Physiology 77.3 (1984): 513-517.