division cellulaire asymétrique a b m. neuron sheath shaft socket
TRANSCRIPT
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Division cellulaire asymétrique
A
B
M
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NeurorétineInjection de HRP
?
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SoieSocleGaineNeuronNeuron
Sheath
Shaft
Socket
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pI
pIIbpIIa
métaphase
télophase
A101 / -tub / Numb
pIpIIb
pIIa
pIIIb
Glial
Neurone
Sheath
Shaft
Socket
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Répartition inégale des déterminants de l’identité cellulaire
A B
1. Polarisation
2. Distribution asymétriquedes déterminants
3. Position du site de clivage(répartition inégale)
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A A
1. Perte de polarité 2. Défaut dans la positiondu site de clivage
A A
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Knoblich, 2002
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pI
pIIbpIIa
métaphase
télophase
A101 / -tub / Numb
pIpIIb
pIIa
pIIIb
Glial
Neurone
Sheath
Shaft
Socket
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pIpIIb
pIIa
pIIIb
Glial
Neurone
Sheath
Shaft
Socket
pIpIIa
pIIa
Socket
Socket
Socket
Socket
pIpIIb
pIIb
Neurone/Glial
Neurone/Glial
Neurone/Glial
Neurone/Glial
wild-type
numbNotch-Act Notch
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wild-type
numb
Notch (ts)
Notch (ts) + numb
Numb
Notch
Jan, 1996
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-adaptin
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Numb
-adaptin
pIpIIa
pIIa
pIpIIb
pIIb
-adaptin Hs-numb
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-adaptin
Notch
pIpIIa
pIIa
pIpIIb
pIIb
-adaptin Notch (ts) - 29°C
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Numb -adaptinNotch
Numb
pIIapIIb
pIIb pIIa
•••
•••
•
Notch
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Pon-GFP
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pI pIepidermal
Pon-GFPHistone2B-YFP
anterior posterior
apical
basal
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FzDsh
PkStbm
Planar polarity in Drosophila
frizzledflamingostrabismus dishevelledprickle
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Polar distribution of planar polarity proteins
FzPk,Stbm
Fz-GFP
pIpI
StbmH2B-YFP
GFP-StbmH2B-YFP
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Planar polarity genes orient pI cells
-tubulin Numb
frizzledflamingostrabismus dishevelledprickle
wild-type
random
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posterioranterior
Fz, Dshorientation(prior to mitosis)
Stbm, Pk
Numb
?
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Les neuroblastes de l’embryon de DrosophileT
emps
VNE
neuroblaste
épiderme
GCM
neurones
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Le déterminant Prospero
Neuroblastespécifique : dpn,ase
GMCspécifique : eve,ftz
sauvage prospero
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Prospero Miranda
Nbs
GMC
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QuickTime™ et un décompresseurNone sont requis pour visualiser
cette image.
The mitotic spindle rotates by 0-90°
QuickTime™ et un décompresseurMotion JPEG A sont requis pour visualiser
cette image.
- Centrosomes are randomly positioned at separation- Centrosomes migrate by the shortest path to orient the spindleNo evidence for centrosome priming
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Identification du gène inscuteable
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Inscuteable se localise au pôle apical
interphase prophase métaphase anaphase
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Inscuteable est requis pour localiser Miranda
Miranda / ADN
Insc
-
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BazDmPKCDmPar6
Inscuteable-Localisationde Miranda-Rotation du fuseau
Gi/Gß
BazDmPKCDmPar6
Inscuteable
Pins-GiGDP
Cibles?
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apical cue
Bazooka (PAR-3)DmPAR-6DaPKC
Inscuteable
Pins
Mitotic spindle rotation
Basal accumulationof Numb and Prospero
Drosophila neuroblasts
Gi-GDP + G
Chia, Doe, Jan, Knoblich, Knust ...
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FzDsh
PkStbm
Planar polarity in Drosophila
frizzledflamingostrabismus dishevelledprickle
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Planar polarity genes orient pI cells
-tubulin Numb
frizzledflamingostrabismus dishevelledprickle
wild-type
random
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BazPAR-6aPKC
PinsGi-GDP
Numb
G effectors aPKC targets
bazwild-type pins
Numb
pins
bazooka and pins are required to localize Numb ...
NumbNumb
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Establishment of polarity in pI cells and in neuroblasts
Planar polarity signal
Dlg-Pins(anterior)
BazookaDaPKCDmPAR-6(posterior)
pI Neuroblast
Apical signal
BazookaDaPKCDmPAR-6(apical)
PinsInscuteable
Numb localises opposite to Baz
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Inscuteable reverts pI cell polarity WT
Numb Insc Numb Pins
WT
UAS-Insc
Insc Numb Pins
Baz Dlg
UAS-Insc
UAS-Insc UAS-Insc
Dlg-Pins
BazookaDaPKCDmPAR-6(anterior)
Numb(posterior)
Inscuteable
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Numb (drosophile-mammifères: 603 aa)
PTB
Neurorétine