dmd #031831 a comparison of whole genome gene expression profiles of heparg cells to human liver...
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DMD #031831
A comparison of whole genome gene expression profiles of HepaRG cells to human liver tissues, primary human
hepatocytes, and HepG2 cells
Steven N. Hart*, Ye Li*, Kaori Nakamoto, Eva-anne Subileau, David Steen, and Xiao-bo Zhong
Supplemental Figure 1S. A comparison of the expression values in Log2 scale among HepG2 cells, differentiated HepaRG cells, and primary human hepatocytes for CYPs in panel A, ADH in panel B, ALDHs in panel C, Non-CYP phase I enzymes in panel D, GSTs in panel E, NATs in panel F, SULTs in panel G, UGTs in panel H, ABCB/ABCC/ABCG in panel I, and SLCOs in panel J. When multiple sets of probes related to a gene are presented on the arrays, the probe that is most variable for a given gene is selected to represent the gene. The bars show the average expression values in Log2 scale and the error bars indicate standard deviation. **p<0.01 and ***p<0.001 are used to indicate a statistically significant difference compared to primary human hepatocytes in a t test. UGT1Aa corresponds to multiple UGT1A family members, including UGT1A1, UGT1A3, UGT1A4, UGT1A8, UGT1A9, and UGT1A10.
Supplemental Figure S1.
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A. CYPs
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ADH1A ADH1B ADH1C ADH4 ADH5 ADH6 ADHFE1
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Exp
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Leve
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B. ADHs
Gen
e ex
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g 2)
Gen
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g 2)
HepG2 Diff HepaRG PHH
Supplemental Figure S1.
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C. ALDHs
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FMO1 FMO2 FMO3 FMO4 FMO5 POR XDH
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D. Non-CYP Phase I
Gen
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Gen
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Supplemental Figure S1.
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E. GSTs
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F. NATs
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Gen
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g 2)
Supplemental Figure S1.
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SULT1A1 SULT1A2 SULT1B1 SULT1C2 SULT1C4 SULT1E1 SULT2A1
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G. SULTs
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H. UGTs
Gen
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g 2)
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a
Gen
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Supplemental Figure S1.
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I. ABCB/ABCC/ABCG
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SLCO1A2 SLCO2B1 SLCO3A1 SLCO4C1 SLCO6A1
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J. SLCOs
Gen
e ex
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(Lo
g 2)
Gen
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g 2)
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