TECHNOLOGY FOR PRODUCING NON-SPREADING SPOTS ON µ- ARRAY

Reducing the surface induced defects in spotting DNA µ-array plates is difficult. A simpler solution to this

problem lies in CD burning technology. The CD commercially available in market, is a transparent disc

with a very thin layer of dye on one of the surfaces. While burning a CD what we actually do is that we

burn the surface of the dye using a laser of very high resolution. When the signal is 1, laser is on & when

signal is 0, laser is off. In a way, we produce well defined microscopic dots on the dye layer on the CD

surface.

Now, in order to apply this technology to DNA µ-array plate development, we need to develop

certain dyes which do not allow other substances to bind with it under normal conditions but becomes a

good binder when denatured using a LASER. So, using LASER, we can actually produce very good circular

binding spots on the DNA µ-array surface & prevent mixing of two spots & surface induced defects on

the spots.

VED PRAKASH

NISER

17/3/2010