dna & cell reproduction
DESCRIPTION
DNA & Cell Reproduction. MODERN BIOLOGY Chapters 10-1 & 8. DNA. What is DNA? Deoxyribonucleic acid or DNA is a long thin molecule (nucleic acid) that stores genetic information. Main Function: - PowerPoint PPT PresentationTRANSCRIPT
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DNA & Cell Reproductio
nMODERN BIOLOGY
Chapters 10-1 & 8
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DNAWhat is DNA?Deoxyribonucleic acid or DNA is a long thin molecule (nucleic acid) that stores genetic information.
Main Function:Store and transmit the genetic information that tells cells which proteins to make and when to make them.l
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Structure of DNA
DNA -The Double Helix
Scientists James D. Watson and Francis Crick determined the structure of DNA in 1953, using the X-ray crystallography work of Rosalind Franklin and Maurice Wilkins that indicated DNA had a helical structure (shapedlike a twisted ladder).
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Structure of DNA
Made of smaller, repeating subunits called NUCLEOTIDES.
Each nucleotide is made of 3 parts:1. Sugar (deoxyribose)2. Phosphate3. 4 different Nitrogenous Bases (A, G, C, T)
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Structure of DNA
*Sugar and phosphate group - same in all nucleotides .
*4 different Nitrogen bases:
Adenine, thymine, guanine and cytosine
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Structure of DNA
The bases, adenine, thymine, guanine, and cytosine, are often labeled A, T, G and C.
Each base always pairs with its ‘partner’ base; referred to as complementary base pairing’.
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Structure of DNA
Complementary Base Pairing
Adenine always bonds with thymine to form a stepCytosine always bonds with guanine to form a stepDeoxyribose sugar and phosphate are the backbone or sides of the ladder
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Structure of DNA
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~FYI~A DNA molecule can have billions of ‘steps’. Approx. six billion in the human cell !!
These steps are made up of different combinations of the base pairs, A, G, T, C.
The number and pattern of pairs form a genetic "code" that makes up genes-the genetic code that controls growth, development and characteristics of an organism.
Using just these four bases, nature can create an almost unlimited variety of genetic messages.http://www.pbs.org/wgbh/nova/genome/media/2809_q_02.htm
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DNA Replication
Cells that divide must pass exactcopies of their DNA to theiroffspring cells.
REPLICATION-the process ofduplicating a DNA molecule.
During replication , the double helixunwinds & separates.Each chain serves as a templatefor a new chain, or strand of DNA.
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DNA Replication
The Steps of DNA Replication:
1. To begin the process, the double helix unwinds. As with all metabolic activities, an enzyme (DNA helicase) is needed for this process.
2. DNA helicase unzips the 2 strands of nucleotides in between the bases – point called the replication fork. Now the bases are exposed.http://highered.mcgraw-hill.com/sites/9834092339/student_view0/chapter14/dna_replication_fork.html
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DNA Replication
The Steps:
3. Complementary nucleotides floating in the nucleus pair with the exposed bases.DNA polymerase (another enzyme!) assists in the addition and bonding of the complimentary nucleotide.
Result: 2 new strands of DNA - each consisting of ½ new DNA and ½ old DNA. (identical to the original)http://highered.mcgraw-hill.com/sites/9834092339/student_view0/chapter14/how_nucleotides_are_added_in_dna_replication.html
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DNA Replication
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http://www.youtube.com/watch?v=hfZ8o9D1tus
DNA REPLICATION WORKSHOP ACTIVITY
http://www.pbs.org/wgbh/aso/tryit/dna/#
http://highered.mcgraw-hill.com/sites/0072943696/student_view0/chapter3/animation__dna_replication__quiz_1_.html
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Accuracy & Repair of DNA
•DNA replication is accurate - usually only 1 error in every 10,000 paired nucleotides•But any error, called a mutation, can have serious effects•Radiation, chemicals , heat can damage DNA (mutation)•Cell has proofreading techniques – like spell check•Over 20 or more repair enzymes fix errors
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http://learn.genetics.utah.edu/content/begin/dna/tour_dna.html
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Cell ReproductionAll cells come from the division of pre-existing cells. Cell division or Cell Reproduction allows for growth and repair, carries on life and passes genetic information to future generations.
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1. Before a cell can divide, the loosely packed genetic material (DNA) in the nucleus of the eukaryotic cell must make exact copies of itself. This loosely packed genetic material (DNA) is referred to as chromatin.
2. Chromatin then coils into very compact structures called chromosomes.
Each chromosomes consists of two identical strands of DNA tightly wrapped around proteins called histones.
Cell Reproduction
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Cell Reproduction
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3. These two identical halves are referred to as chromatids (or ‘sister chromatids’)
Centromere - Holds the two chromatids together.
4. During division, sister chromatids from each chromosome separate and each new cell will get one.
Chromosomes
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Cell Reproduction
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Every Species has a different number of chromosomesHumans have 46 chromosomes (23 pairs)Human and animal chromosomes are either:• Autosomes• sex chromosomes
44 autosomes (do not determine the sex)2 sex chromosomes (determine the sex)
Females have 2 X chromosomesMales have an X and a Y
chromosome
Chromosome Numbers
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Every cell of an organism produced by sexual reproduction has two copies of each autosome Autosomal Homologous chromosomes (sometimes called homologues) are the same size and shape and carry genes for the same traits.
Chromosome Numbers
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KARYOTYPE:*An arranged photomicrograph of chromosomes. *Shows the number of chromosomes, and what they look like under a microscope.*Helps determine problems in chromosomes. **Attention is paid to: chromosome number & length
position of the centromeresbanding pattern sex chromosomes
Chromosome Numbers
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Trisomy 21 – Down Syndrome•3 copies of chromosome 21 instead of 2•1 in 900 births•More common with older mothers•Some impairment of cognitive ability and physical growth•A particular set of facial characteristics
Chromosome Numbers
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Cells having two sets of chromosomesare said to be diploid (2n).Diploid - having both chromosome for each homologous pair.All human cells (somatic), except reproductive cells (sperm & egg) are diploid cells. 46 - 22 pairs homologous chromosomes & 2 sex chromosomes.
Chromosome Numbers
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Cells with only one of the two chromosomes is considered haploid (1n)Sperm and egg cells are haploid.Haploid cells have half the number of chromosomes that are present in diploid cells.When two haploid cells combine, each new cell will be diploid.
Chromosome Numbers
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Chromosomes: Eukaryote vs. Prokaryote
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Every Cell has a life of its own!Every Cell comes from pre-existing cells!•single-celled organisms•billion of cells that make up the bodies of plants and animals.Each cell has:•A beginning (growth)•A middle (genetic material replicates and cell prepares for cell division)•And an end (Mitosis & Cytokinesis )Voila - TWO NEW CELLS!!
Cell Division
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Cell division - the process by which cells produce offspring cells
It is very different for prokaryotes and eukaryotes
Cell Division: Eukaryote vs. Prokaryote
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Cell Division: Eukaryote vs. Prokaryote
Cell Division in Prokaryotes:Binary fission – division of a prokaryotic cell into two offspring •Single chromosome copies itself•Cell grows to 2X its size•Cell wall forms between the 2 chromosomes•Each new cell is identical
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Cell Division: Eukaryote vs. Prokaryote
Cell division in eukaryotesMitosis – •Division of a cell’s nucleus that produces a genetically identical cell•It does not change the amount of DNA in a cell•Mitosis occurs in the reproduction of unicellular organisms and in the addition of cells to a tissue or organ of a multicellular organism.
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Cell Division: The Cell Cycle
The Cell Cycle is the process or stages cells go through to grow, copy their DNA, and divide to make new cells.
The cycle starts when a cell is formed and ends when a cell divides to make new cells.
http://www.cellsalive.com/cell_cycle.htm
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Cell Division: The Cell Cycle
The Cycle consists of:Interphase – time between cell divisionMitosis – nucleus of cell dividesCytokinesis – division of cytoplasm
= 2 new cells!
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Cell Division: The Cell Cycle
InterphaseTakes up 80-90% of a cells lifespanThree distinct parts:•G1 (Growth Phase) – cell doubles in size, organelles double in number•S (Synthesis Phase) – chromatin (DNA) replicates•G2 (Growth Phase) - rapid growth phase & cell check *prepare for next division•G0 (resting phase) – muscles cells, retina cells, nerve cells – those you don’t need a lot of go into a resting phase
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Mitosis•Chromosomes separate• Nucleus divides There are four important stages of Mitosis:1.Prophase2.Metaphase3.Anaphase4.Telophase
Cell Division: The Cell Cycle
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The Four Stages of Mitosis Mnemonic
“(P)LEASE” - Prophase
“(M)AKE” - Metaphase
“(A)NOTHER” - Anaphase
“(T)WIN” - Telophase
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1.Prophase
•Chromatin coils and forms chromosomes•nucleolus and nuclear membrane disappear•centrosomes and spindle fibers appear.•Chromosomes can be seen under a microscope.
Cell Division: Mitosis
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2.Metaphase spindle fibers assist in aligning all chromosomes at the center of the cell.
Cell Division: Mitosis
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3.Anaphase Spindle fibers pull chromosomes apart.
One set of chromosomes is pull toward one pole of the new cell and the other set toward the other.
Cell Division: Mitosis
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4.Telophase
•Centrioles and spindle fibers disappear.•Middle of an animal cell pinches together.•A nuclear membrane forms around each new set of chromosomes.•In plant cells, a new cell wall and a new cell membrane form down the middle of the cell.
Cell Division: Mitosis
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Mitosis in Plant Cells
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5.Cytokinesis•Cytoplasm and organelles split and form two daughter cells – exactly like the parent cell•Each cell receives an identical copy of the original cell’s chromosomes and half of the cytoplasm and organelles
Cell Division: Mitosis
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Please watch this video
Now Take the Mitosis Quiz
Record your results
Special thanks to
Cell Division: Mitosis
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Body cells (somatic cells) are reproduced through mitosis. The number of chromosomes remain the same in each daughter cell produced.
However, there is another type of cell division called Meiosis. The number of chromosomes is cut in half in all daughter cells produced.
Cell Division: Meiosis
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Meiosis – Called the “Reduction Division” •Type of cell division that reduces the # of chromosomes (diploid - 2n) in each new cell to half of the original # (haploid - 1n)
•Meiosis produces sex cells (sperm and egg cells), each with that reduced number of chromosomes.
Cell Division: Meiosis
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When mitosis is completed, each new cell's chromosome number remains the same as in the original parent cell (diploid – 2n).
When meiosis is completed, each sex cell's chromosome number is half (haploid- 1n). The sperm and egg cells have half the number of chromosomes that are found in a normal body cell (somatic cell).
Cell Division: Meiosis
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Sex Cells, also called gametes fuse during sexual reproduction and form a zygote with a normal # of chromosomes
Cell Division: Meiosis
spermegg
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Found in testes and ovaries of humans
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Meiosis actually involves two separate cell divisions, and they occur one right after the other.
Meiosis I
Meiosis II
Cell Division: Meiosis
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Meiosis I: Similar to Mitosis I1.Prophase I: DNA has already duplicated. Chromosomes become visible. The nuclear membrane disappears. Spindle fibers appear. Centrosomes (containing centrioles) begin to move. THEN…Synapsis – pairing ofHomologous chromosomes – does not occur in mitosis(Every chromosome lines up withits homolog and forms a tetrad.)
Cell Division: Meiosis
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1.Prophase I:When the homologous chromosomes form a tetrad, chromatids can exchange pieces, called crossing – over
Genetic recombination results
Meiosis – Helping Genetic Diversity
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Meiosis I: Similar to Mitosis I2.Metaphase I:• Tetrads line up randomly along the
midline of the cell• Spindle fibers attach to centromeres
and poles of cell
Cell Division: Meiosis
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Meiosis I: Similar to Mitosis I3.Anaphase I:• Each homologous chromosome moves
to an opposite pole of the dividing cell
Cell Division: Meiosis
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Meiosis I: Similar to Mitosis I• Independent assortment – the random
separation of homologous chromosomes
• Genetic recombination occurs again due to random separation of maternal (mom’s) and paternal (dad’s) chromosomes
Cell Division: Meiosis
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Meiosis I: Similar to Mitosis I4.Telophase I:•Chromosomes reach the opposite ends of the cell - Cytokinesis begins•New cells are now haploid (1n)
• they contain only one homologue from each homologous pair
• But they have two copies of each chromosome because the DNA copied itself right before beginning Meiosis I
Cell Division: Meiosis
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Meiosis II1.Prophase II: chromosomes DO NOT duplicate. If a nuclear membrane formed, it will break apart again.
2.Metaphase II: chromosomes line up in the center of the cell
3.Anaphase II: sister chromatids split and separate.
4.Telophase II: cells pinch together. Nuclear membranes form. Four daughter cells are formed. Each cell has only half the number of chromosomes as the parent cell.
Cell Division: Meiosis
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Gamete Formation• Meiosis produces haploid reproductive
cells called gametes• Meiosis only occurs in cells of the
reproductive organs• Humans:
• Oogenesis - female ovaries produce 1 egg and 3 polar bodies (disintegrate)
• Spermatogenesis - male testes produce 4 sperm
Cell Division: Meiosis
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Oogenesis
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Spermatogenesis
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Take a look at a family with more than one child. You can see the importance of meiosis in genetic variation. The children are different from each other even though they have the same parents. With the exception of identical twins, offspring from the same family never look exactly alike.
~FYI~
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Because of genetic recombination during meiosis, sexual reproduction results in offspring that are different from each other and from their parents.
FYI
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Meiosis
Great Meiosis Video!
Animated video of Meiosis!
Now, Take the Meiosis Quiz!
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Mitosis
Interphase Prophase Metaphase
Anaphase
Telophase
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Meiosis
Prophase I
Anaphase I
Metaphase I
Telophase I
Prophase II
Metaphase II
Anaphase II
Telophase II
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Mitosis vs. Meiosis
Mitosis vs. Meiosis Interactive
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Production of offspring from one parent:• no union of gametes• no genetic diversity• offspring genetically identical
(clone)• Unicellular organisms –binary fission
(prokaryotes; i.e. bacteria) or mitosis (eukaryotes; i.e. algae, some yeasts, and protozoans, such as paramecium) budding, too
• Multicellular organisms – budding (i.e. hydra)
Asexual Reproduction
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• Except in identical twins, offspring are unique combinations of their parents’ genes
Sexual Reproduction
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Sexual reproduction is the formation of a new individual following the union of two gametes. Sexual reproduction gives an evolutionary advantage in an environment that can change – “survival of the fittest”
Sexual Reproduction