dna holds the instructions when proteins are produced in the body, the instructions for how to make...
TRANSCRIPT
DNA holds the instructions
When proteins are produced in the body, the instructions for how to make it comes from the DNA (deoxyribonucleic acid)
BUT, there are many steps between a strand of DNA and a protein
Let’s review DNA first• DNA is double-stranded
• Adenine is always across from Thymine
• Cytosine is always across from Guanine
• bases are held across from each other by weak hydrogen bonds
A--T
C--G
Making Proteins•Making proteins involves another step as you saw on the first slide
•First, a molecule called RNA is produced
•This process is called TRANSCRIPTION
The nucleotides of RNAThey are slightly different than DNA
They have a sugar and a phosphate group like DNA does
The difference
Three of the nitrogenous bases are the same: Adenine (A) Cytosine (C) Guanine (G)
The last one is different: Uracil (U)
Instructions for a ProteinThe instructions to make a certain protein are
coded by the sequence of nucleotides in a strand of DNA (a gene)
These instructions get transferred from the DNA strand to an RNA strand through transcription
So how does this happen?
There are a number of steps in the process of making a protein
The first involves two different parts: RNA polymerase the gene’s promoter
A promoter is a certain order of DNA
that acts as a “start” signal for transcription
RNA polymeraseRNA polymerase is an enzyme
It binds to the promoter of a gene
This enzyme helps unwind and separate the two strands of DNA
The templateOne strand of the DNA acts as a “template” for
making the RNA
This means that the nucleotides that will form the RNA pair up with this strand
RNA polymerase helps make this happen
Base Pairing Rules Just like with DNA, the nucleotides only pair
with certain other nucleotides
If there is a Thymine on the DNA strand, Adenine still pairs with it
If there is an Adenine on the DNA strand, it now pairs with Uracil
Cytosine pairs with Guanine just like before
Some covalent bonds are involved
As the RNA nucleotides get added to the strand of RNA that is forming, they are forming covalent bonds
The covalent bonds occur between the nucleotides of the RNA
How it endsTranscription keeps going until RNA polymerase
reaches a “stop” signal on the DNA
RNA polymerase falls off of the DNA and releases the RNA molecule
The DNA then winds back up
Animations:http://www.dnalc.org/resources/3d/12-transcript
ion-basic.html
http://www.youtube.com/watch?v=OtYz_3rkvPk
Textbook: http://bcs.whfreeman.com/thelifewire/content/chp12/1202001.html
Nebraska: http://croptechnology.unl.edu/animation/transcription.swf