JBP±JbaseDNA

JBP ±JbaseDNA

Revised data analysis Janu

ary 2010

JBP sequ

ence coverage

JBP H/D

 excha

nge

0‐10

10‐20

20‐30

30‐40

40‐50

50‐60

60‐70

70‐80

80‐90

90‐100

JBP H/D

 excha

nge ±DNA

Sequ

ence

zStart

End

 % Change

 Std

VYD

SATLRD

GKG

RVIGVM

350

67‐1

1YD

SATLRD

GKG

RVIGVM

251

67‐1

1YD

SATLRD

GKG

RVIGVM

351

67‐1

2YD

SATLRD

GKG

RVIGVM

451

67‐2

1LRDGKG

RVIG

256

65‐1

1LRDGKG

RVIGVM

256

671

1

YAEYRR

RLAAAQQDSTAQPV

VS

3272

293

‐11

YAEYRR

RLAAAQQDSTAQPV

VSSV

2272

295

‐31

YAEYRR

RLAAAQQDSTAQPV

VSSV

3272

295

‐21

AEYRR

RLAAAQQDSTAQPV

VSSV

2273

295

‐11

AEYRR

RLAAAQQDSTAQPV

VSSV

3273

295

‐11

YRRR

LAAAQQDSTAQPV

VS

3275

293

‐12

YRRR

LAAAQQDSTAQPV

VSS

3275

294

‐12

LRDGKG

RVIGVM

256

67‐1

1LRKA

LPEH

ATTAASG

L2

6883

01

LRKA

LPEH

ATTAASG

L3

6883

‐11

LRKA

LPEH

ATTAASG

LLSA

268

86‐1

1LRKA

LPEH

ATTAASG

LLSA

368

86‐1

1LRKA

LPEH

ATTAASG

LLSA

468

86‐2

2RK

ALPEH

ATTAASG

L2

6983

‐12

RKALPEH

ATTAASG

L3

6983

‐22

YRRR

LAAAQQDSTAQPV

VSS

3275

294

‐12

YRRR

LAAAQQDSTAQPV

VSSV

3275

295

‐12

RRRLAAAQQDSTAQPV

VSS

2276

294

‐11

RRRLAAAQQDSTAQPV

VSS

3276

294

01

RRRLAAAQQDSTAQPV

VSSV

3276

295

‐11

SVVEKPN

GKN

LYKP

STVFPIDPTPFAVV

3294

321

‐11

VVEKPN

GKN

LYKP

STVF

3295

311

‐12

VVEKPN

GKN

LYKP

STVF

4295

311

02

VVEKPN

GKN

LYKP

STVFPIDPTPFAVV

3295

321

11

RKALPEH

ATTAASG

LLSA

269

86‐1

1RK

ALPEH

ATTAASG

LLSA

369

86‐1

1AAVRTSL

287

931

3VRTSLRSSM

289

97‐4

4FG

GESPLSG

IAGY

198

110

‐11

FGGESPLSG

IAGY

298

110

01

FGGESPLSG

IAGYF

198

111

‐21

FGGESPLSG

IAGYF

298

111

‐22

VVEKPN

GKN

LYKP

STVFPIDPTPFAVV

3295

321

‐11

VVEKPN

GKN

LYKP

STVFPIDPTPFAVV

4295

321

‐11

VVEKPN

GKN

LYKP

STVFPIDPTPFAVVAQL

3295

324

‐11

VVEKPN

GKN

LYKP

STVFPIDPTPFAVVAQL

4295

324

‐11

VEKPN

GKN

LYKP

STVFPIDPTPFAVV

2296

321

01

VEKPN

GKN

LYKP

STVFPIDPTPFAVV

3296

321

01

VEKPN

GKN

LYKP

STVFPIDPTPFAVV

4296

321

‐11

PIDPTPFAVVAQL

2312

324

‐11

DYRGSPVE

2112

119

‐22

DYRGSPVEL

1112

120

‐22

KARK

TAF

1121

127

‐13

KARK

TAF

2121

127

‐41

KARK

TAFT

2121

128

‐21

IYKSVMPEHWAAQ

3148

160

‐11

IYKSVMPEHWAAQD

3148

161

‐11

IYKSVMPEHWAAQDSA

IPDIVRIHGTPFSTL

3148

178

‐10

AQLHRLHHCA

AKG

LCVHEL

2322

340

11

AQLHRLHHCA

AKG

LCVHEL

3322

340

01

AQLHRLHHCA

AKG

LCVHEL

4322

340

01

HRLHHCA

AKG

LC2

325

336

03

HRLHHCA

AKG

LCV

3325

337

‐12

LLAVPSSPLAVL

2340

351

‐13

LAVPSSPLAVL

1341

351

02

LAVPSSPLAVL

2341

351

02

IYKSVMPEHWAAQDSA

IPDIVRIHGTPFSTL

3148

178

‐10

WAAQDSA

IPDIVRIHGTPFSTL

3157

178

‐10

AAQDSA

IPDIVRIHGTPFSTL

3158

178

01

DSA

IPDIVRIHGTPFSTL

3161

178

‐10

SAIPDIVRIHGTPFSTL

2162

178

‐10

SAIPDIVRIHGTPFSTL

3162

178

‐11

TINSRF

2179

184

03

TINSRFRTA

SHTD

AGDFD

GGYSC

2179

201

‐11

TINSRFRTA

SHTD

AGDFD

GGYSC

3179

201

11

LAVPSSPLAVL

2341

351

02

LAVPSSPLAVLL

1341

352

01

LAVPSSPLAVLL

2341

352

‐22

LFGERL

2352

357

03

FGERL

2353

357

‐33

TTAVE

1397

401

12

TTAVEKSKY

2397

405

12

TTAVEKSKYLERDHLSQC

2397

414

12

TTAVEKSKYLERDHLSQC

3397

414

12

TINSRFRTA

SHTD

AGDFD

GGYSC

3179

201

‐11

TINSRFRTA

SHTD

AGDFD

GGYSCI

3179

202

12

CIDGDFKG

2204

211

‐21

IDGDFKG

2205

211

‐12

IDGDFKGLALGFD

DF

2205

219

‐11

KGLALGFD

DF

2210

219

‐12

HVNVPM

QPR

DVL

2220

231

‐10

VFD

SHYFHSN

SE2

232

243

‐12

TTAVEKSKYLERDHLSQC

3397

414

12

TTAVEKSKYLERDHLSQC

4397

414

01

TTAVEKSKYLERDHLSQCIS

2397

416

11

WVEA

RKHWLRL

2423

433

‐20

WVEA

RKHWLRL

3423

433

‐20

VATEWARM

2434

441

‐23

4IATA

PERTDF

1442

451

‐52

IATA

PERTDF

2442

451

‐82

VFD

SHYFHSN

SE3

232

243

‐11

VFD

SHYFHSN

SEL

1232

244

‐11

VFD

SHYFHSN

SEL

2232

244

‐10

VFD

SHYFHSN

SEL

3232

244

‐21

YYRSALGEPSS

2261

271

‐42

IATA

PERTDFL

1442

452

‐42

IATA

PERTDFL

2442

452

‐82

IATA

PERTDFLWKN

KSPM

NTA

F3

442

463

‐91

LWKN

KSPM

NTA

F3

452

463

‐12

2WKN

KSPM

N2

453

460

‐52

FDLCE

1464

468

‐41

VAKQ

VM

2469

474

‐41

VAKQ

VMLGL

2469

477

‐32

LGLLDKETA

TPTEERHF

3475

491

11

LGLLDKETA

TPTEERHF

4475

491

03

LGLLDKETA

TPTEERHFW

SVY

2475

495

‐11

LGLLDKETA

TPTEERHFW

SVY

4475

495

‐22

VVETDVQCR

Q2

732

741

‐12

CRQYFTLHYPLRVQY

3739

753

‐22

RQYFTLHYPLRVQY

3740

753

‐11

DAVAPA

F1

754

760

11

LGLLDKETA

TPTEERHFW

SVY

4475

495

‐22

LDKETA

TPTEERHF

2478

491

21

LDKETA

TPTEERHF

3478

491

22

LDKETA

TPTEERHFW

SVY

2478

495

‐11

LDKETA

TPTEERHFW

SVY

3478

495

01

AAHLHRA

CA1

496

504

‐32

AAHLHRA

CA2

496

504

‐71

AAHLHRA

CAE

1496

505

‐41

296

09

DAVAPA

F1

754

760

11

CARYKLQGSPMNT

2791

803

‐11

ARYKLQGSPMNT

2792

803

‐11

ARYKLQGSPMNTV

VHL

3792

807

‐11

AAHLHRA

CAE

2496

505

‐91

HRA

CAE

2500

505

‐92

HRA

CAERLM

M3

500

509

‐52

ERLM

MPEEA

2505

513

‐45

PEEA

MS

1510

515

‐24

6LRKLNVKLKD

3516

525

‐30

2FSFG

GTRY

2526

533

‐83

DYQ

TED

1578

583

‐32

DYQ

TEDC

1578

584

‐31

YQTEDCE

1579

585

‐32

EVDYA

GHGWAVPK

QHAKTVTA

NVHQEA

VA

3585

613

01

WAVPK

QHAKTVTA

3593

605

‐11

2VAATTEA

1612

618

‐37

VVLPRP

PSGDRG

DAAVDLPKE

3623

643

01

VVLPRP

PSGDRG

DAAVDLPKE

4623

643

11

VVLPRP

PSGDRG

DAAVDLPKEVTTSA

EW3

623

650

01

VVLPRP

PSGDRG

DAAVDLPKEVTTSA

EW3

623

650

01

VVLPRP

PSGDRG

DAAVDLPKEVTTSA

EW4

623

650

01

VDLPKE

2638

643

13

FAYH

DSLPQ

KATFD

2685

698

21

FAYH

DSLPQ

KATFD

3685

698

22

FAYH

DSLPQ

KATFDF

3685

699

01

AYH

DSLPQ

KATFD

2686

698

22

AYH

DSLPQ

KATFDF

3686

699

12

FVVLQ

HVLSAM

2699

709

11

FVVLQ

HVLSAM

2699

709

‐11

FVVLQ

HVLSAMPEDA

2699

713

01

FVVLQ

HVLSAMPEDAIA

2699

715

00

VVLQ

HVLSAMPEDA

2700

713

01

VVLQ

HVLSAMPEDAIATD

YVSRMRSICTG

CL3

700

730

00

IATD

Y1

714

718

‐13

IATD

YVSRM

2714

722

12

YVSRM

2718

722

‐11

RSICTG

CL2

723

730

13

FVVETDVQ

1731

738

02

FVVETDVQCR

Q2

731

741

01

FVVETDVQCR

QYFTLHYPLRVQY

2731

753

‐20

FVVETDVQCR

QYFTLHYPLRVQY

3731

753

‐21

FVVETDVQCR

QYFTLHYPLRVQY

4731

753

‐21

434-

441

(+2)

100

Apo_JBP

***

516-

525

(+3)

100

Apo_JBP

***

406080JBP_ligand

***

% HDX

406080JBP_ligand

***

% HDX

0.1

1

10

100

1000

1000

0

020

0.1

1

10

100

1000

1000

0

020

Tim

e (s

)Ti

me

(s)

452-

463

(+3)

593-

605

(+3)

()

6080100

Apo_JBP

JBP_ligand

***

HDX

()

6080100

Apo_JBP

JBP_ligand

***

HDX

1

1

0

00

00

0002040

% H

1

1

0

00

00

00

02040% H

0.1

1

10

100

1000

1000

0

Tim

e (s

)

0.1

1

10

100

1000

1000

0

Tim

e (s

)

434-

441

(+2)

80100

Apo_JBP

JBP_ligand

***

X

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

442-

451

(+2)

80100

Apo_JBP

JBP_ligand

***

X

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

442-

451

(+1)

80100

Apo_JBP

JBP_ligand

**X

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

442-

452

(+1)

80100

Apo_JBP

JBP_ligand

*X

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

442-

452

(+2)

80100

Apo_JBP

JBP_ligand

***

X

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

452-

463

(+3)

80100

Apo_JBP

JBP_ligand

***

X

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

453-

460

(+2)

80100

Apo_JBP

JBP_ligand

**X

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

496-

504

(+1)

80100

Apo_JBP

JBP_ligand

nsX

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

496-

504

(+2)

80100

Apo_JBP

JBP_ligand

***

X

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

496-

505

(+1)

80100

Apo_JBP

JBP_ligand

**X

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

496-

505

(+2)

80100

Apo_JBP

JBP_ligand

***

X

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

500-

505

(+2)

80100

Apo_JBP

JBP_ligand

***

X

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

500-

509

(+3)

80100

Apo_JBP

JBP_ligand

**X

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

510-

515

(+1)

80100

120

Apo_JBP

JBP_ligand

***

X

20406080

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

516-

525

(+3)

80100

Apo_JBP

JBP_ligand

***

X

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)

593-

605

(+3)

80100

Apo_JBP

JBP_ligand

***

X

204060

% HDX

0.1

1

10

100

1000

1000

0

0

Tim

e (s

)(

)