dna sequencing troubleshooting web resource · dna sequencing research group overview • designed...
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DNA Sequencing Research Group
DNA Sequencing Troubleshooting Web Resource
DNA Sequencing Research Group
Peter Schweitzer, Debbie Adam, David Needleman, Michelle Detwiler, Helaman
Escobar, Jan Kieleczawa, Rashmi Pershad, Glenis Wiebe, and Susan Hardin
Doug HexterWorld Design Group, Inc.
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DNA Sequencing Research Group
Overview• Designed for Facility Personnel
– Many of us have static troubleshooting pages on our websites. These are primarily designed to assist users, and primarily for template/primer problems. The DSRG DNA Sequencing Troubleshooting Web Resource is designed to do this, but also benefit facility personnel (i.e. it includes information on instrument errors in addition to template/chemistry problems).
• Users Populate this Database– The Web Resource contains records of problems (often with
solutions) submitted by the public. It was designed to be flexible to keep up with new instruments, new chemistries, and new techniques.
• This is a Problem-centric database– Solutions are not needed to make a submission, and users
may submit solutions to previously-submitted problems.
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DNA Sequencing Research Group
URL and links
• URL: http://www.abrf.org/index.cfm/stwr.home
• Linked from the ABRF DSRG web site
www.ABRF.org/DSRG
• Add a link from your own home page!
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DNA Sequencing Research Group
Front Page/Simple Search
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DNA Sequencing Research Group
Front Page/Simple Search
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DNA Sequencing Research Group
Advanced Search
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DNA Sequencing Research Group
Advanced Search
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DNA Sequencing Research Group
Front Page/Browse All
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DNA Sequencing Research Group
Browse All
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DNA Sequencing Research Group
Browse All
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DNA Sequencing Research Group
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DNA Sequencing Research Group
To view or download image
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DNA Sequencing Research Group
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DNA Sequencing Research Group
Problem Solution
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DNA Sequencing Research Group
Add comment or suggest a solution
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DNA Sequencing Research Group
Front Page
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DNA Sequencing Research Group
New Problem Submission
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DNA Sequencing Research Group
New Problem Submission
We do require this, though this information is not made public
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DNA Sequencing Research Group
New Problem Submission
This is the field searched by the Keyword search
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DNA Sequencing Research Group
New Problem Submission
Browse to upload your image file (if necessary)
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DNA Sequencing Research Group
New Problem Submission
Only other required fields are:
Instrument modelTemplate type (N/A is option)Sequencing chemistry (N/A is option)
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DNA Sequencing Research Group
Curation by the DSRG
• DSRG receives an email when submission is made—the record is designated “pending” and not made public.
• The DSRG will examine each record to determine whether to:– Remove identifying information (e.g. from the .ab1 file)– Reformat image file (usually to .pdf)– Collect additional information and or additional images/data
from the submitter that we feel is needed to complete the record
– Make the Record Public
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DNA Sequencing Research Group
Final Request
• This database will only be as good as the submissions (and solutions) received.
• Please submit your problems. Don’t be concerned that they may be redundant with others we have. We, the DSRG, will determine if yours is a better example, or is not needed. We’d rather have too many submissions than too few!
• Remember—you don’t need a solution to submit a problem!