879982
Serum miR-146a, miR-155 and miR-210 as potential markers of Graves disease
Lei Zheng a, b , Chunbo Zhuang a, b , Xiaobei Wang b, * , Liang Ming a, *
a
Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Zhengzhou University, No. 1 Jian She East Road, Zhengzhou, Henan, P. R. China.
b
Department of Clinical Laboratory Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1227 Jie Fang Road, Wuhan, Hubei, P. R. China
*
Corresponding author. Liang Ming, email: [email protected], Tel: +86037166913118; Fax: +86037166913118. Xiaobei Wang, email: [email protected], Tel: +8602785726310; Fax: +8602785726310.
Treg-associated circulating miRNAs in Graves' disease
Abstract
Background: Previous studies have demonstrated that dysfunctional regulatory T cells (Tregs) may be associated with Graves disease (GD). In this study, we evaluated four serum Treg-associated miRNAs (miR-210, miR-182, miR-155 and miR-146a) expressions and assessed the potential of serum miRNAs as biomarkers of GD. Methods: Foxp3 and serum miRNAs expressions both in GD patients and healthy controls were measured by RT-PCR. Results: Serum miR-210 in GD patients was significantly higher than healthy controls (2.64-fold, P0.055%
250
1
2
3
4
5
6
7
8
246
247
248
249
250
(X
cv
6.7
5.2
4.8
8.1
5.0
5.1
4.2
5.9
4.6
4.9
5.9
6.2
7.1
5.1
2.5
6.4
5.0
4.9
7.9
4.6
5.2
4.0
5.8
4.5
4.9
5.8
6.1
7.3
4.9
2.4
%
4.6
4
2.0
2.5
2.5
1.9
5.0
1.7
2.0
0
1.7
1.6
2.7
4.0
4
3 H9
4
[6]HBA1C4HBA1CLPLCHBA1C[8]HBA1C[3-4]HPLC4500 [5]HPLCAHPLC [6]EDTA-K2HbA1c2-8 []HbA1c[8]11HbA1cHbA1c[9][10]
[1] [J] 201037(19)p3719-3720
[2] [J]20141240p55-56
[3] [J] 201661313 p1839-1840
[4] [J] 20129918p967-968
[5] [J] 201553610p1458-1459
[6] [J] 275200910p465-466
[] [J]2p967-968
[8] [J]2009299p805-807
[9] [J] 2012396p714-716
[10] [J] 20133 243p224-225
872133
The reference intervals for HE4, CA125 and ROMA in healthy female
Objectives: HE4 is a tumor marker in diagnosing ovarian cancer. Together with CA125 and ROMA score, it can improve sensitivity and specificity. There are no reports about the serum HE4 reference intervals (RIs) with electrochemiluminescence immunoassay (ECLIA). The RI of ROMA hasnt been established yet. In this study, we establish the RIs for serum HE4, CA125 and ROMA in healthy Chinese female with ECLIA.Methods: Serum HE4 and CA125 concentrations in healthy female were simultaneous measured with ECLIA on Roche Cobas E601 system. The RIs were determined following CLSI C28-A3 guidelines using a nonparametric method.Results: The upper limits of the 95% percentile intervals were 82.62pmol/L for HE4, 30.91 U/ml for CA125 and 19.27 for ROMA. The reference limits for HE4, CA125 and ROMA were 65.87pmol/L, 32.23 U/ml, 13.14 for premenopausal women, and 90.76pmol/L, 27.52 U/ml, 25.46 for postmenopausal women respectively.Conclusion: We established the RIs for serum HE4, CA125 and ROMA with ECLIA in healthy Chinese female. It provided a reference for clinical and laboratory studies.
875749
450052
MethylenetetrahydrofolatereductaseMTHFRtotalhomocysteinetHcyfolateB6VitaminB6,VitB6B12VitaminB12,VitB12age-related cataract,ARC127ARC129tHcyenzyme-linked immuno sorbent assay,ELISAMTHFRVitB6VitB12ROCARC1ARCMTHFRP0.05tHcyP0.052ROCMTHFRtHcy5ARCAUC0.689,0.689,0.732ARCAUC0.8250.8050.855ARCAUC0.7060.7420.8115ARCMTHFRtHcyARC
ROCB6B12MTHFRtHcy
Diagnosis value of plasma indexes of homocystine metabolism for ARC and its subtypes
Abstract: Objective To investigate the diagnostic accuracy of homocystine(Hcy) metabolismindexes, such as MTHFR enzyme activity, total homocystine (tHcy), folic acid (FA), vitaminB6 and Vitamin B12 in age-related cataract (ARC) and its subtypes. Methods In 127 ARC patients and 129 controls, the plasma tHcy levels were detected by an automated assay, while concentrations of MTHFR, folate, vitamin B6, B12 were measured by ELISA. The diagnostic accuracy of each index and combined indexes was assessed by area under the receiver operating characteristics (ROC) curves (AUCs). Results (1) Plasma concentrations of MTHFR activity and FA were significantly lower, while tHcy levels were significantly higher in ARC patients than those in the control group (P0.05). (2)The area under ROC curves (AUC) of MTHFR, tHcy, and combined five indexes above were 0.689, 0.689, 0.732 for the diagnosis of ARC; 0.825, 0.805, 0.855 for the diagnosis of cortical ARC; 0.706, 0.742, 0.811 for the diagnosis of posterior subcapsular ARC. Conclusion: The combination of five indexes could improve the diagnosis efficiency of ARC, whilethe MTHFR activity and tHcy levels could be useful diagnostic markers for cortical ARC and posterior subcapsular ARC,
Key words: age-related cataract, ROC curve, folate, Hcy, MTHFR, vitamin B6, vitamin B12
Age-related cataract, ARC16001[]reactive oxygen speciesROSARC2[]totalhomocysteinetHcyROStHcy ADDIN EN.CITE
[3]MethylenetetrahydrofolatereductaseMTHFRB6VitaminB6,VitB6B12VitaminB12,VitB12tHcyHcyMTHFRtHcyVitB6VitB12ARCARC
1
1.1 20141020154127ARC12915020/40ARCLens opacities classification system LocsARCLocs4Locs2Locs2ARC ADDIN EN.CITE
[4]
1
N
129
6046.5
6953.5
68.54.9
58-86
ARC
127
5442.5
7357.5
68.79.1
52-84
43
1330.2
3069.8
69.79.5
50-84
32
1959.3
1340.7
67.68.8
51-82
23
1043.5
1356.5
67.68.3
56-84
29
1241.4
1758.6
69.39.7
51-84
1.2 EDTA2000g512000r/min10-807020tHcyELISAMTHFRVitB6VitB12ELISA
2
SPSS17.0Graphpad 6.0tP0.051-ROCAUCP95%95%CI=0.05
3
3.1ARCMTHFRtHcyP0.05VitB6VitB12ARCP0.05ARCMTHFRtHcyARCARCP0.054P0.05VitB12ARCVitB12P0.052.
2ARC5
ARC
Mean SD
Mean SD
Mean SD
Mean SD
Mean SD
Mean SD
MTHFRU/L
370.0 51.0
337.6 35.7*
312.8 27.6*
356.8 28.0
334.5 32.5*
355.9 34.1
nmol/L
16.2 1.7
15.0 2.7*
15.4 2.4*
14.5 2.1*
15.0 3.5*
15.0 3.1*
tHcymol/L
10.8 1.9
12.3 2.5*
13.4 2.6*
11.5 2.4
12.4 1.8*
11.6 2.4
VitaminB12pmol/L
271.4 37.7
267.4 30.9
273.0 28.2
265.5 26.1
254.2 38.9*
271.7 30.5
VitaminB6nmol/L
41.6 5.1
41.4 5.2
41.9 5.5
40.8 5.0
39.8 5.7
42.6 4.9
*P0.05
3.2 ARCROC
MTHFRtHcyVitB6VitB12ROC195% CIP%%3MTHFRtHcyVitB6VitB12ROCAUC0.6890.6890.546,0.5120.521MTHFRtHcyVitB6389.82U/L12.67mol/L13.21nmol/L44.70nmol/L300.56pmol/L92.1%39.5%48.4%81.4%23.6%97.7%76.4%31.0%84.3%26.4%MTHFRARCARC
ARCMTHFRtHcyARCARCARCVitB6VitB12ARC
3.3 5ARC
ROC1ARCARCARCAUC0.7320.8550.81180.3%81.4%78.3%72.1%77.5%74.6%ARC
1MTHFRtHcy5ROC
AMTHFRtHcy5ARCROCBMTHFRtHcy5ARCROCCMTHFRtHcy5ARCROC
3:ARC
ARC
ARC
ARC
AUC(95%CI)
%
%
AUC(95%CI)
%
%
AUC(95%CI)
%
%
MTHFR
0.689(0.624-0.754)
92.1
39.5
0.825(0.764-0.887)
93.0
62.8
0.706(0.609-0.803)
91.3
47.3
tHcy
0.689(0.624-0.754)
48.8
81.4
0.805(0.719-0.891)
72.1
81.4
0.742(0.644-0.840)
87.0
62.8
FA
0.546(0.472-0.619)
23.6
97.7
0.502(0.388-0.615)
32.6
82.9
0.656(0.500-0.813)
60.9
76.0
VitB6
0.512(0.441-0.583)
76.4
31.0
0.509(0.408-0.611)
11.6
96.9
0.596(0.471-0.720)
91.3
31.0
VitB12
0.521(0.450-0.593)
84.3
26.4
0.525(0.435-0.616)
88.4
26.4
0.613(0.488-0.738)
56.5
65.9
MTHFR+tHcy+FA+VitB6+VitB12
0.732(0.671-0.793)
80.3
72.1
0.855(0.797-0.913)
81.4
77.5
0.811(0.719-0.902)
78.3
74.6
4
MTHFRtHcyVitB6VitB12ROCARC5ARCMTHFRtHcyARCARC
tHcyROSARC ADDIN EN.CITE
[5]TantHcyARC ADDIN EN.CITE
[3]ROCtHcyARCARCARCtHcyROSARC ADDIN EN.CITE
[6]tHcyARCMTHFRtHcytHcytHcy ADDIN EN.CITE
[7]ARCMTHFRP0.05ROCMTHFRARCARC
VitB6VitB12tHcytHcy8[]1ARC24% ADDIN EN.CITE
[3]ARCtHcyP0.05ROCARC97.7%ARCBARC ADDIN EN.CITE
[9]metaARC ADDIN EN.CITE
[10]ARC VitB12P0.05ROCVitB6VitB12ARC
ARC5ARCARCARCARCARC
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[2]BERTHOUD V M, BEYER E C. Oxidative stress, lens gap junctions, and cataracts [J]. Antioxidants & redox signaling, 2009, 11(2): 339-53.
[3]TAN A G, MITCHELL P, ROCHTCHINA E, et al. Serum homocysteine, vitamin B12, and folate, and the prevalence and incidence of posterior subcapsular cataract [J]. Investigative ophthalmology & visual science, 2015, 56(1): 216-20.
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[6]IKESUGI K, YAMAMOTO R, MULHERN M L, et al. Role of the unfolded protein response (UPR) in cataract formation [J]. Experimental eye research, 2006, 83(3): 508-16.
[7]QIN X, LI Y, YUAN H, et al. Relationship of MTHFR gene 677C --> T polymorphism, homocysteine, and estimated glomerular filtration rate levels with the risk of new-onset diabetes [J]. Medicine, 2015, 94(7): e563.
[8]ZHANG S, SHI C, MAO C, et al. Plasma Homocysteine, Vitamin B12 and Folate Levels in Multiple System Atrophy: A Case-Control Study [J]. PloS one, 2015, 10(8): e0136468.
[9]GLASER T S, DOSS L E, SHIH G, et al. The Association of Dietary Lutein plus Zeaxanthin and B Vitamins with Cataracts in the Age-Related Eye Disease Study: AREDS Report No. 37 [J]. Ophthalmology, 2015, 122(7): 1471-9.
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876484
PCAFEZH2
EZH2H327H3K27EZH2PRC2EEDSUZ12RbAp46/48EZH2XEZH2EZH2EZH2EZH2 487CDK1EZH2EZH2O--N-EZH2EZH2EZH2Western blotCo-IPReal-time PCRChIPTranswell/EZH2EZH2EZH2PCAFSIRT1PCAFEZH2EZH2K348K348EZH2 345487EZH2PRC2K348EZH2EZH2 K348EZH2EZH2EZH2
882369
graphitic carbon nitrideg-C3N4ATetrabromobisphenolAg-C3N4 g-C3N4 XXEISN-TBBPA g-C3N4g-C3N4-g-C3N4g-C3N4TBBPA0.02 M - 1 M5 nM g-C3N4TBBPAg-C3N4g-C3N4A
882666
455000
::3840:M78.9%MMIgG2257.89%IgA718.42%IgM615.79%37.89%MMP