Download - ACC Lab Setup
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Fatma AlqudsiAnimal cell and tissue culture Bio357
Small tissue culture laboratory
Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.
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Fatma Alqudsi Animal cell and tissue cultureBio 357
Medium sized tissue culture
laboratory
Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.
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Fatma Alqudsi Animal cell and tissue cultureBio 357
Tissue culture laboratory with adjacent room
Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.
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Fatma Alqudsi Animal cell and tissue cultureBio 357
Large tissue culture laboratory
Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.
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Fatma Alqudsi Animal cell and tissue cultureBio 357
Hot Room
Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.
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Fatma Alqudsi Animal cell and tissue cultureBio 357
Washing upsink
Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.
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Fatma Alqudsi Animal cell and tissue cultureBio 357
Liquid nitrogen
store
Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.
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Fatma AlqudsiAnimal cell and tissue culture Bio357
Minimum requirements Desirable features Useful additions
Sterile area, clean, quiet, and with no
through traffic
Filtered air (air-
conditioning)Piped CO2 and compressed air
Separate from animal house and
microbiological labs
Service bench adjacent to
culture areaStoreroom for bulk plastics
Preparation area Separate prep room Quarantine room
Wash up area (not necessarily within
tissue culture laboratory, but at leastadjacent to it)
Hot room with temperature
recorder
Containment room (could
double as quarantine room)
Space for incubator(s) Separate sterilizing room
Liquid N2 storage tank (~500 L)
and separate storeroom for
nitrogen freezers
Storage areas: Separate cylinder store Microscope room
Liquids: ambient, 4C, 20C Darkroom
Glassware (shelving) Vacuum line
Plastics (shelving)
Small items (drawers)
Specialized equipment (slow turnover),
cupboard(s)
Chemicals: ambient, 4C, 20C; share
with liquids, but keep chemicals in
sealed container over desiccant
CO2 cylinders
Space for liquid N2 freezer(s)
Sink
Tissue culture facilities
Culture of Animal Cells: A Manual of Basic Technique Published by John Wiley & Sons, Inc.
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Fatma Alqudsi Animal cell and tissue cultureBio 357
Equipment of tissue
culture Laboratory
Equipment of tissueEquipment of tissue
culture Laboratoryculture Laboratory
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Fatma Alqudsi Animal cell and tissue cultureBio 357
Requirements of a tissue
culture laboratory
1. Laminar-flow hood
2. Sterilizing oven3. Autoclave4. Centrifuge
5. Balance6. Cell counter7. Inverted microscope8. Dissecting microscope
9.Humid Co2 incubator10.Incubator11.pH meter12.Hotplate magnetic
stirrer13.Magnetic stirrer14.Glassware15.Refrigerator
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Fatma Alqudsi Animal cell and tissue cultureBio 357
http://www.pemed.com/lab/hoods/forma110401_47.jpg
Laminar-flow hood
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Fatma Alqudsi Animal cell and tissue cultureBio 357
http://www.geol.ku.dk/pershps/robertfrei/WEB/laminair-flow-hood-1.jpg
Laminar-flow hood
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Fatma Alqudsi Animal cell and tissue cultureBio 357
Inverted microscope
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Fatma Alqudsi Animal cell and tissue cultureBio 357
http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artjul98/invert.html
Inverted microscope
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Fatma Alqudsi Animal cell and tissue cultureBio 357
Inverted microscopehttp://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artjul98/invert.html
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Fatma Alqudsi Animal cell and tissue cultureBio 357
A device in which solid or liquid
particles of different densities are
separated by rotating them in a
tube in a horizontal circle. The
denser particles tend to move
along the length of the tube to agreater radius of rotation,
displacing the lighter particles to
the other end.
html.centrifuge/C/.../info.daviddarling.www
Centrifuge
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Fatma Alqudsi Animal cell and tissue cultureBio 357
https://reader009.{domain}/reader009/html5/0523/5b04c15f85524
Centrifuge