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Bio 328 Immunology
Immunoglobulins: structure and Function
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+
-
Albumin
globulin
globulin
globulin
Origin
Inflammation Normal Normal Myeloma
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Arne Tiselius and Elvin Kabat 1939.
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2014)
1972 Noble Prize in Medicine.
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Porter et al.
Nisonoff et al.
Edelman et al.
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Goat anti-Human Fab binds to both L- and H-chain; goat antiHuman Fc binds
only to H-chain.
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Angiotensin ll
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Van der Waals surface contact between angiotensin ll and Fab fragment.
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Flu virus antigen and antibody
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Fab and Influenza virus antigen
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Anti-HIV protease Fab
+ peptide
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Fab different anti-hen egg lysozyme
Fab different small epitopes
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Unbound Ag-bound
Induced-fit
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Unbound Ag-bound
Anti-hemagglutinin Fab and peptide.
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Antibody –Mediated Effector Functions
1.Ab-medicated clearance of pathogensa) Neutralizationb) Opsonizationc) Complement activationd) ADCC
2.Ab isotypes or classes mediate different functions3.Fc Receptors
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How do Antibodies kill Cancer Cells?
1.Binding to cell surface receptors and preventing their activation.2.Interference with vascularization.3.Induction of apoptosis.4.Induction of ADCC.5.Induction of Complement fixation.6.Delivery of toxins.
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IgG, IgA, IgD IgM, IgE
CH1/CH1CH1/CH1
Hinge regionCH2/CH2
CH2/CH2CH3/CH3
CH3/CH3CH4/CH4
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1921 K. Prausnitz and H. Kustner1966 K and Y Ishizaka
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Immunoreceptor Tyrosine Activation Motifs
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B-cell Linker Protein
Bruton’s tyrosine kinase
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X-linked agammaglobulinemia
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The End