LC/MS/MS Method Package forAminoglycoside Antibiotics
For LabSolutions™ LCMS Software
Complete Solution for Quantitation and Con�rmation of Aminoglycosides in Meat, Eggs and Milk
Aminoglycosides are an antibiotic family widely used for the treatment of bacterial infections in cattle, sheep, pigs and poultry. Regulatory
agencies have set maximum residue limits (MRL) for these compounds after veterinary use. Aminoglycosides are very polar compounds, and
usually require the use of undesirable ion-pairing reagents with LC-MS/MS.
This Method Package is a complete solution for the simultaneous quantitative analysis of all regulated aminoglycosides by HILIC/MS/MS.
It includes:
• A sample preparation protocol using mixed-mode weak cation exchange from Biotage. This protocol produces high recoveries and low matrix
effects, and is adapted to meat, milk or egg samples.
• A primary method for fast (< 5 min) quantitative analysis by HILIC/MS/MS with two MRMs per compound.
• A second method for con�rmation of identity. Using the same mobile phases and column, the gradient is modi�ed and 15 optimized MRMs are
acquired per compound. This enables library searching and accurate identi�cation on Insight Screening using MRM Spectrum Mode (library provided).
LCMS-8060
C146-E352
• Complete method for fast quantitative screening of 13 aminoglycosides in meat, eggs and milk.
• Increase identi�cation con�dence with MRM Spectrum mode and LabSolutions Insight™ Screening.
• No ion-pairing reagent required.• Includes single sample preparation protocol usable for
meat, eggs and milk.
• Amikacin
• Apramycin
• Dihydrostreptomycin
• Gentamicin (as Gentamicin C1, C1a and C2/C2a)
• Hygromycin B
• Kanamycin
• Neomycin B
• Netilmicin
• Paromomycin
• Sisomicin
• Spectinomycin
• Streptomycin
• Tobramycin
• Ribostamycin (internal standard)
Targeted Aminoglycosides:
Streptomycin
LC/MS/MS Method Package for Aminoglycoside AntibioticsFor LabSolutions LCMS Software
Easy Work�ow
Complete protocol includes step-by-step instructions for sample and calibration curve preparation.
Fast Quantitation and Easy Processing
Perform high-sensitivity analysis with excellent chromatography without
resorting to ion-pairing reagents that foul the mass spectrometer.
LabSolutions Insight provides a fast and intuitive environment for data
processing and review. User-con�gurable �ags alert you what values
are outside of QC limits, facilitating review by exception.
Increased Con�dence in Identi�cation using
MRM Spectrum Mode
MRM Spectrum Mode acquires up to 15 MRMs per analyte, and each
transition is optimized to a speci�c collision energy. Because MS duty
cycle is not wasted on meaningless portions of the spectrum,
information-rich spectra can be acquired in just milliseconds. The
result is an unambiguous spectrum, even for trace-level components.
Note
1. LabSolutions LCMS Ver.5.91 or later is required.
2. LabSolutions Insight Library Screening Ver. 3.1 or later is required.
LabSolutions Insight and LabSolutions are trademarks of Shimadzu Corporation.
Biotage logo is a registered trademark of Biotage AB.
© Shimadzu Corporation, 2018First Edition: April 2018, Printed in Japan 3655-12704-30AIT
Homogenization Dilution
Extraction Analysis Data ReviewPuri�cation