CoolCell Container Alcohol-Filled ContainerNo alcohol • Nofluids• Nopre-coolingrequired

Requires 250ml alcohol• Replacealcoholevery5uses• Keeptrackofnumberofuses• Pre-coolalcoholinrefrigerator

No variability• Allvialshaveuniformfreezerate• Radiallysymmetricdesignensuresconsistency

Inconsistent freeze rate• Alcoholdegradationinducesvariability• Twocirclesofwells–differentfreezeratesforeach

No on-going cost• Noalcoholpurchaseordisposal

Approximately $350/year• Changealcoholweekly• Disposalofhazardouswaste

No stuck lids• Lidcomesoffeasilywhenfrozen• Notcoldtothetouchwhenremovingfromthe

-80oCfreezer

Difficult to handle• Screwcapverydifficulttoremovewhenfrozen• Frozenunitisslipperyandcoldtotouch

Quick re-use time• Readytouseagainafterfiveminutes

Wait between runs• Takes>1hourfordevicetoreturntoroomtempera-

tureforre-useLow impact on freezer• 1/3theheatimpactonfreezercomparedto

alcohol-filledunits

Large thermal mass impacts local freezer area• Largeheatcapacityremovedfromalcoholimpacts

nearbysamples

CoolCell® Alcohol-Free Cell Freezing

CoolCell alcohol-free cell freezing containersensurestandardizedcontrolled-rate-1oC/minutecellfreezingina-80oCfreezer-withoutalcoholoranyfluids.Provenforusewithavarietyofcelltypesincludingstemcells,primarycellsandcelllines.Thepatent-pendingCoolCelltechnologyutilizesathermo-conductivealloyandhighly-insulativeoutermaterialstocontroltherateofheatremovalandmakecellcryopreservationreproducible.CoolCellunitsareeasytouse;simplyfillwithcryogenicvialsandplaceina-80oCfreezer.

Why Alcohol-Free?

• Consistent-1oC/minutefreezingrate

• Novariabilityfromfreezetofreeze,moreconsistent,fasterresults

• Highercellviability

• Nomaintenance,nowaste

• Nohiddencost

• Easytouse

• Minimalimpactonfreezerandfrozencontents

CoolCell ConsistencyTestimonials

“WerunaregistryinwhichlargeamountsofPeripheralBloodMononuclearCellsareprocessedforlong-termcryopreser-vation.AftertestingtheCoolCell,wefoundslightlybettercellviability(>90%)thanwithourcurrentcellfreezingcontainers,andthereisnoisopropanolwastegenerated.Overall,theCoolCellhassetanewbarincryopreservation.”

-RohitG.,Stanford University

Fiveconsecutivefreezecyclesshowhighlyreproduciblefreezingprofiles.

Cryopreservation and Thawing of Cells Yokoyama WM, Thompson ML, and Ehrhardt ROCurrentProtocolsinImmunology,Nov2012

To Order, call 1.888.478.2221 or visit www.biocision.com

CoolCell FTS30utilizesanovelmetalalloyandcontrolledmicro-convectiontechnologytoevenlydrawin-80°Cfreezerairthroughabottombasevent,uniformlydispersethecoldairaroundeachvialinthecentralchamberusingadiffuserplateandthenreleasethethermalloadfromthevialsthroughatopventchoke.Theinnervialmoduleholds30cryogenicvialsandcanberemovedinonestep.

Eachvialachievesauniformandreproducible-1°C/minutefreezingprofile.Thevial-to-vialandcycle-to-cyclethermalprofilesarehighlyreproduciblewhenthesamevialloadandfreezertemperatureisapplied.

Duetothelowthermalmassoftheuniform-densitycrosslinkedpolyethyleneinsulationfoamcontainer,freezingcanbeconductedwithoutariseinlocalfreezertemperature,therebyprotectingnearbysamples.

ThelowthermalmassalsoensuresthatCoolCellFTS30containerswillrapidlyreturntoroomtemperatureforanotherfreezingcycle.

How It Works

CoolCell Performance

Fig 1.HESC-RC10.After2weeksinLN2,3vialsfromeachmethodwerethawedandcellscountedimmediately(Day1)andafter3daysgrowth(Day3).CoolCellmethodshowed33%increaseincellsafter3daysgrowth.Fig 2. AlamarBluereductionassayforproliferationanalysisofRC10cells.CellsfrozeninCoolCellgrewmorequicklyleadingtohigherproliferationratesandmorecells.

Stem Cells

Primary Cells

Cell Lines

Fig 5. HeLA,CHO-K,K562,NIH3T3.CoolCell12-well,CoolCellFTS3030-welland“Mr.Frosty”freezingcon-tainerswereusedtofreezeallfourcelllines.Identicaltransfectionefficienciesandviabilitieswereobservedafterthawing.Fig 6.Identicalgrowthofcellswasobserved24hourspost-thaw.

Fig 3.PBMC.AfterLN2storage,vialsfrozeninCoolCelland“Mr.Frosty”isopropanolcontainerswerecompared.Cellswerethawedandviabilitycountswereobtainedusingtrypanbluestainmethod.Resultsweresimilar,althoughCoolCellrequirednoalcoholandnomaintenance.Fig 4. HUVECsuspensionsfrozeninCoolCelloriso-propanolcontainer(5each)wererapidlythawedandresuspendedingrowthmediaandlivecellcountswereobtainedbytrypanblueexclusionmethod.CoolCellsignificantlyoutperformedisopropanolcontainer.

Fig 1 Fig 2

Fig 3 Fig 4

Fig 5 Fig 6

HumanEmbryonicStemPreadipccytesBreastCancerStemColonCancerStemGliablastomaCancerStemMouseEmbryonicStemHumanEndothelialProgenitor

NeonatalKeratinocytesHumanWBCsMouseWBCsHumanCD34+MuscleHumanTendonFibroblastsMelanomaTumorHumanCardiacVentricularHumanCardiacAtrial

CoolCell LX incombinationwitha-80°Cfreezer,willprovidethefreezingrateof-1°C/minutethatisidealforcryopreservationofmostculturedcelllines.Thecontainerusesacombinationofuniform-densitycrosslinkedpoly-ethyleneinsulationfoam,ametalalloycorering,andradialvialsymmetry.Asaresult,freezingprofilesareconsistentfromoneruntothenext.

ItisimportanttofullyloadCoolCellcontainerspriortofreezing.

TheCoolCellLXfoamretainsverylittleheatwhichensuresthatthecon-tainerwillimposenegligiblechangeinthefreezerenvironmentandprotectnearbyfrozensamples.ThelowheatcontentalsoensuresthatCoolCellLXcontainerswillrapidlyreturntoroomtemperatureforanotherfreezingcycle.

CHOLnCapHTB77A549HeLa

LidLid

Chamber

Top vent choke

Core Diffuser plate

Base

Base

Chamber

Vial module

Base vent

CoolCell 5mL LX For12x3.5to5mLcryogenicvials,3.5to5mLfillpervial.Radially-symmetricforuniformvialfreezing.Numberedwellsforeasysampleidentification.Beveledlidforsecuregrippingandeasyopening.Exposedvialtopswhenlidisopenforquick,organizedremovaloffrozensamples.

ItemNo. Description DimensionsBCS-406 ● CoolCell5mlLX,purple Diam9.5xH14.5cm.

CoolCell SV10 6x10mLserumvials,5mLfillpervial.Radially-symmetricforuniformfreezingofinjectableserumvials.Easyopenlid.Exposedvialtopswhenlidisopenforquick,organizedremovaloffrozensamples.

CoolCell LX Cell Freezing Container

CoolCell Cell Freezing Container

CoolCell 5ml LX Cell Freezing Container

CoolCell FTS30 High-Capacity Cell Freezing Container

CoolCell SV Cell Freezing Containers for Injectable Vials

CoolCell LX For12x1mLor2mLcryogenicvials,1mLfillpervial.Radially-symmet-ricforuniformvialfreezing.Numberedwellsforeasysampleidentifi-cation.Beveledlidforsecuregrippingandeasyopening.Exposedvialtopswhenlidisopenforquick,organizedremovaloffrozensamples.

CoolCell FTS30 For30x1mLor2mLcryogenicvials,1mLfillvolumepervial.Controlledmicro-convectionforuniformfreezingof30vials.Removablecryogenicvialtrayforone-steptransferofsamplesintoandoutoffreezingchamber.

ItemNo. Description DimensionBCS-170 ● CoolCellFTS30,purple Diam16.5xH11.5cm.BCS-170G ● CoolCellFTS30,greenBCS-170OBCS-170PK

● CoolCellFTS30,orange● CoolCellFTS30,pink

ItemNo. Description DimensionsBCS-405 ● CoolCellLX,purple Diam11.7xH10.9cm.BCS-405G ● CoolCellLX,greenBCS-405O ● CoolCellLX,orangeBCS-405PK ● CoolCellLX,pink

CoolCell SV2 12x2mLinjectableserumvials,1mLfillpervial.Radially-sym-metricforuniformfreezingofinjectableserumvials.Easyopenlid.Exposedvialtopswhenlidisopenforquick,orga-nizedremovaloffrozensamples.

ItemNo. Description DimensionsBCS-172 ● CoolCellSV2,purple Diam11.7xH10.9cm.

ItemNo. Description DimensionsBCS-262 ● CoolCellSV10,purple Diam11.7xH10.9cm.

To Order, call 1.888.478.2221 or visit www.biocision.com

CoolCell For12x1mLor2mLcryogenicvials,1mLfillpervial.Economical,controlled-ratecellfreezingcontainer.Radially-symmetricforuniformvialfreezing.Easyopenlid.

ItemNo. Description DimensionsBCS-136 ● CoolCell,purple Diam11.7xH10.9cm.BCS-136G ● CoolCell,greenBCS-136O ● CoolCell,orangeBCS-136P ● CoolCell,pink

Note:ItisimportanttofullyloadeachCoolCellunitpriortofreezing.CoolCellFillervials(page5)arerecommendedforfillinganyemptywells.

CoolCell Protecting Agents Addyourserum,mediaorbufferstothesesingle-usecellbiologygradeDMSOorglycerolcryoprotectants.EliminateswastinglargebottlesofDMSOanddifficultpipettingofviscousglycerol.Dualgraduations-percentageandmL-onthebackformeasuring.Preparefrom5%(50mL)to20%(12.5mL)ofcryopreservationsolution.

ItemNo. Description

BCS-3010 CoolCellProtectingAgent,DMSOBCS-3011 CoolCellProtectingAgent,Glycerol

Cell Cryopreservation Accessories

CryoCeps™ Cryogenic Vial Grippers Cryogenicvialgrippersfeatureauniquedesigntograspinternal-orexternal-threadcryogenicvials.Easilysortormovevialswhileprotectingfingersfromdryiceandliquidnitrogen.2perpack.

ItemNo. Description

BCS-213 ● CryoCeps,green

ItemNo. Description

BCS-3105 CoolCellFillerVial,2ml 6perpackBCS-3106 CoolCellFillerVial,5ml 6perpack

CoolCell Filler Vial Toensurecellfreezingrateconsistencyanduniformresults,insertaCoolCellFillerVialintoemptywellswhenfreezinglessthanafullload.Filledwithglycerolandsuitableforrepeateduse.CompatiblewithCoolCellLX,CoolCell,CoolCellFTS30andCoolCell5mlLX.

TruCool® Cryogenic Vials Availablewithinternalorexternalthreadsforasecure,leak-proofseal.Thermally-fusedgasketlayeroneachcapreplacestraditionalO-ring.Eachtubeisindividuallybarcodedforaccurateidentificationandanonymity.

InternalThreadsItemNo.

ExternalThreadsItemNo.

Description

BCS-2410 BCS-2400 1mL,Self-StandingBCS-2411 BCS-2402 2mL,Self-StandingBCS-2412 BCS-2401 2mL,RoundBottom__ BCS-2403 3mL,Self-StandingBCS-2413 __ 4mL,RoundBottomBCS-2414 BCS-2404 4mL,Self-StandingBCS-2415 __ 5mL,RoundBottomBCS-2416 BCS-2405 5mL,Self-Standing

ItemNo. Description

BCS-2425 ● Capinserts,whiteBCS-2420 ● Capinserts,blueBCS-2421 ● Capinserts,greenBCS-2423 ● Capinserts,redBCS-2424 ● Capinserts,yellowBCS-2422 ● Capinserts,pinkBCS-2426 Capinserts,assorted

CapInserts,600perpack

TruCool® Hinged CryoBoxes Patentedhingedlidoffersconvenienceandarchivalintegrity,ensuringmarkingsandvialsremaininsync.Lidstaysattachedtobaseminimiz-ingriskoflidcontamination,andiseasytoopenwhenfrozen.Plastic81-placegridhasadjustableslats.Holds811mLor2mLcryogenicvialsandmicrofugetubes.Availableinpacksof5or50.Dimensions:L13.3xW13.3xH5.1cm(5.25x5.25x2in).5perpack 50perpack Description

BCS-206 BCS-207 ● HingedCryoBox,whiteBCS-206B BCS-207B ● HingedCryoBox,blueBCS-206G BCS-207G ● HingedCryoBox,greenBCS-206O BCS-207O ● HingedCryoBox,orangeBCS-206P BCS-207P ● HingedCryoBox,purpleBCS-206PK BCS-207PK ● HingedCryoBox,pinkBCS-206MC __ HingedCryoBox,multi-color(nowhite)