![Page 1: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/1.jpg)
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?Steven RobertsSchool of Aquatic and Fishery Sciencesrobertslab.info@sr320
![Page 2: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/2.jpg)
Shameless plug
onsnetwork.org/eimd
@EIMD_fhl
![Page 3: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/3.jpg)
Shameless plug
onsnetwork.org/eimd
@EIMD_fhl
![Page 4: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/4.jpg)
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
![Page 5: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/5.jpg)
Open Science
• You are free to Share!
• Our lab practices open notebookscience
• Data, Preprints, Proposals, Slidedecks available FigShare, GitHub, and lab website (robertslab.info)
These slides plus links @ robertslab.info
![Page 6: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/6.jpg)
commercially important traits
Lab Background
![Page 7: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/7.jpg)
commercially important traits
modified from
Lab Background
![Page 8: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/8.jpg)
modified from
![Page 9: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/9.jpg)
modified from
![Page 10: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/10.jpg)
modified from
![Page 11: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/11.jpg)
Today
modified from
1
23
![Page 12: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/12.jpg)
![Page 13: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/13.jpg)
Histone Modification
Epigenetics
short RNAs
DNA Methylation
![Page 14: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/14.jpg)
Photo credit: Flickr, Creative Commons, he-boden
Epigenetics
![Page 15: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/15.jpg)
Epigenetics
![Page 16: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/16.jpg)
![Page 17: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/17.jpg)
m
m
DNA Methylation
![Page 18: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/18.jpg)
Function?
![Page 19: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/19.jpg)
![Page 20: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/20.jpg)
![Page 21: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/21.jpg)
![Page 22: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/22.jpg)
![Page 23: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/23.jpg)
Non-Vertebrates?
landscape and function is very different than what is observed in vertebrates
![Page 24: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/24.jpg)
Non-Vertebrates?
landscape and function is very different than what is observed in vertebrates
![Page 25: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/25.jpg)
Non-Vertebrates?
landscape and function is very different than what is observed in vertebrates
![Page 26: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/26.jpg)
Absent in severalmodel
organisms
![Page 27: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/27.jpg)
Function?Oysters?
![Page 28: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/28.jpg)
![Page 29: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/29.jpg)
![Page 30: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/30.jpg)
mosaic
associated with gene bodies
![Page 31: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/31.jpg)
![Page 32: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/32.jpg)
![Page 33: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/33.jpg)
Roberts and Gavery 2012
in silico analysis
![Page 34: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/34.jpg)
Roberts and Gavery 2012
in silico analysis
![Page 35: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/35.jpg)
Roberts and Gavery 2012
in silico analysis
![Page 36: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/36.jpg)
Two Lineages-
Sperm + Larvae
(72h & 120h)
Claire Olson
Whole Genome BS-Seq
![Page 37: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/37.jpg)
Claire Olson
Differentially Methylated Loci predominant in Transposable Elements
![Page 38: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/38.jpg)
Claire Olsongithub.com/sr320/olson-dev
![Page 39: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/39.jpg)
Summary
• Sparsely (~16 %) methylated genome
• Gene body methylation• Function specific
• TEs are not hypermethylated across genome• Preliminary evidence indicates DMRs are predominant in TEs
![Page 40: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/40.jpg)
![Page 41: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/41.jpg)
![Page 42: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/42.jpg)
![Page 43: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/43.jpg)
Role?
![Page 44: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/44.jpg)
In species that experience a diverse range of environmental conditions, processes have evolved to
increase the number of potential phenotypes in a population in order to improve the chances for an
individual’s survival.
![Page 45: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/45.jpg)
Roberts and Gavery 2012
in silico analysis
![Page 46: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/46.jpg)
Stochastic Variation
housekeeping response to change
![Page 47: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/47.jpg)
Mackenzie Gavery
![Page 48: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/48.jpg)
housekeeping
response to change
![Page 49: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/49.jpg)
Targeted Regulation
![Page 50: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/50.jpg)
Mackenzie Gavery
![Page 51: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/51.jpg)
Mackenzie Gavery
![Page 52: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/52.jpg)
![Page 53: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/53.jpg)
![Page 54: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/54.jpg)
![Page 55: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/55.jpg)
Transposable Elements providing increased diversity?
![Page 56: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/56.jpg)
modified from
Beginning totest these
Hypotheses
![Page 57: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/57.jpg)
Very new dataEnvironment and gene expression
stochastic or targeted?
![Page 58: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/58.jpg)
Very new dataEnvironmental impact (Estrogens)
![Page 59: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/59.jpg)
Very new dataOcean AcidificationSelectionEnvironmental Impact
Katie Lotterhos
![Page 60: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/60.jpg)
Very new dataHeritabilityPlasticityLocal Adaptation
Geneticsversus
Epigenetics
Common Garden Experiment
![Page 61: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/61.jpg)
Acknowledgements
Mackenzie GaveryClaire Ellis
Sam White
Bill HoweDan Halperin
EPASTARDNA methylation
robertslab.infoslides, data & more @
![Page 62: Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?](https://reader034.vdocuments.net/reader034/viewer/2022051817/5485c680b4af9fbd5d8b4a61/html5/thumbnails/62.jpg)