8/19/2019 Dr. Bhawna c Reactive Protein

1/15

C REACTIVE PROTEIN

8/19/2019 Dr. Bhawna c Reactive Protein

2/15

• CRP was discovered in Oswald Avery’s

laboratory - Streptococcus pneumoniaeinfection.

• CRP, named for its capacity to precipitate the

somatic C-polysaccharide of Streptococcus pneumoniae.

•   The rst acute-phase protein to bedescribed and is an important sensitivesystemic marker of inammation and tissuedamae.

8/19/2019 Dr. Bhawna c Reactive Protein

3/15

• !onspecic "cute phase protien

•

#evels rise in the body in responseto$

o %nfection

o

%nammatory responseo Tissue damae

8/19/2019 Dr. Bhawna c Reactive Protein

4/15

&TR'CT'R( )* CRP

• +elons to the pentraxin family ofcalcium dependent liand-bindinplasma proteins.

•  The human CRP molecule iscomposed of ve identicalnonlycosylated polypeptide

subunits, each containin aminoacids.

8/19/2019 Dr. Bhawna c Reactive Protein

5/15

Production

•  This is the early and rapid hostresponse to tissue in/ury.

• #ocal e0pansion of pathoen number

• 1irect activation of compliment intissues.

• 1eranulation of mast cells

8/19/2019 Dr. Bhawna c Reactive Protein

6/15

  Release of inammatorymediators

  &ystemically activemediators$

2%#-3, %#-, T!*-45

  %nitiate production of CRP inliver

  "ctivation of C6(+P ene attranscri tion level

8/19/2019 Dr. Bhawna c Reactive Protein

7/15

Release of CRP in circulation

+inds with PHOSPHOCHOINE ! onbacterial cell wall and damaed eukaryoticcells. 

• "ctivation of classical complimant pathway

• %nteract with % receptors to activate %7

  P8"7)C9T)&%& )* +"CT(R%"6 1":"7(1C(##&

8/19/2019 Dr. Bhawna c Reactive Protein

8/15

8/19/2019 Dr. Bhawna c Reactive Protein

9/15

• %n healthy youn adult the medianconcentration of CRP ;. m6l.

• *ollowin an acute-phase stimulus,values may increase upto 3,-fold.

• 8epatic synthesis starts very rapidlyafter a sinle stimulus.

C#%!%C"# "PP#%C"T%)!

8/19/2019 Dr. Bhawna c Reactive Protein

10/15

• &erum concentrations rise above <m6l by about hours and peaksaround => hours.

•  The plasma half-life of CRP is about3? hours and is constant under allconditions of health and disease.

8/19/2019 Dr. Bhawna c Reactive Protein

11/15

8/19/2019 Dr. Bhawna c Reactive Protein

12/15

C#%!%C"# '&(& )* CRP

o &creenin infection6 inammation6tissuedamae.

)besity6 hypertension- risk of C81

o  :onitorin of the response to treatment of inammationand infection.

o 1etection of intercurrent infection in immunocompromised

individuals.

o 1ianosis of meninitis- bacterial6 viral by detection of CRPin C&*.

8/19/2019 Dr. Bhawna c Reactive Protein

13/15

R"!7( )* CRP #(@(#&

• @%R"# %!*(CT%)!&$ A=m6l

•

+"CT(R%"# %!*(CT%)!&$ =- m6l

• &(@(R( +"CT(R%"# %!*(CT%)!&6

 TR"':"6 +'R!&$ B m6l

8/19/2019 Dr. Bhawna c Reactive Protein

14/15

#"+)R"T)R9 1(T(CT%)!)* CRP

• #ate0 alutination test

• 8s CRP hih sensitivity CRP

• %::'#%T( automated analyDer 21ianosticProduct Corporation5 is a two-sitechemiluminescent enDyme immunometric

assay. with a detection limit of .3 m6#and a measurin rane of .3

8/19/2019 Dr. Bhawna c Reactive Protein

15/15

• +!" nephelometer 21ade +ehrin5by particle-enhancedimmunonephelometry with a

detection limit of .3> m6# and ameasurin rane of .3>33