Download - Effectiveness of Bispecific Cytotoxin (DTEGFATF) in Ameliorating Human Glioblastoma Tumors
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Effectiveness of Bispecific Cytotoxin (DTEGFATF) in Ameliorating Human Glioblastoma Tumors
Travis Spangler2008-2009
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Research Problem
http://upload.wikimedia.org/wikipedia/commons/c/c0/Glioblastoma_-_MR_sagittal_with_contrast.jpg
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Background: Stish et al. (2007)
Diphtheria toxin epidermal growth factor 13
DTEGF13
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DTEGF13
DTEGFIL13DTIL13
Diphtheria toxin amino acid transfer
Diphtheria toxin epidermal growth factor
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Background: Stish et al. (2007)
Diphtheria toxin epidermal growth factor 13
DTEGF13
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DTEGFATF ATF
Diphtheria toxin epidermal growth factor amino terminal fragment
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DTEGFATF ATF
DTEGFATFDTAT
Diphtheria toxin amino acid transfer
Diphtheria toxin epidermal growth factor
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Background: Stish et al. (2008)
DTEGF
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Background: Stish et al. (2008)
DTEGF
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Background: Vallera et al. (2002)
DTAT
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Background: Vallera et al. (2002)
DTAT
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http://www.biooncology.com/bioonc/images/her-main.jpg
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Receptor
http://www.biooncology.com/bioonc/images/her-main.jpg
Receptor
Receptor
Receptor
Receptor ReceptorReceptor
Receptor
Receptor
Receptor
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• DTEGFATF• DTEGF• DTAT
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• DTEGFATF• DTEGF• DTAT• DTEGF + DTAT
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Cell Lines
U-87 HUVEC HPB-MLT
http://www.assaysoft.com/gallery/02Microscopy/MicroscopyGallery/12/Human%20Umbilical%20Vein%20Endothelial%20Cells%20(HUVEC).jpghttp://data-integrator.com
http://www.hpb.org.uk/userimages/panfigure2.jpg
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Cell Lines
U-87 HUVEC HPB-MLT
http://www.assaysoft.com/gallery/02Microscopy/MicroscopyGallery/12/Human%20Umbilical%20Vein%20Endothelial%20Cells%20(HUVEC).jpghttp://data-integrator.com
http://www.hpb.org.uk/userimages/panfigure2.jpg
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Cell Lines
U-87 HUVEC HPB-MLT
http://www.assaysoft.com/gallery/02Microscopy/MicroscopyGallery/12/Human%20Umbilical%20Vein%20Endothelial%20Cells%20(HUVEC).jpghttp://data-integrator.com
http://www.hpb.org.uk/userimages/panfigure2.jpg
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Cell Lines
U-87 HUVEC HPB-MLT
http://www.assaysoft.com/gallery/02Microscopy/MicroscopyGallery/12/Human%20Umbilical%20Vein%20Endothelial%20Cells%20(HUVEC).jpghttp://data-integrator.com
http://www.hpb.org.uk/userimages/panfigure2.jpg
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Proliferation Assays
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Proliferation Assays
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Proliferation Assays
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Proliferation Assays
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Proliferation Assays
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Proliferation Assay Results
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Proliferation Assay Results
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Proliferation Assay Results
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Proliferation Assay Results
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Proliferation Assay Results
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Proliferation Assay Results
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Proliferation Assay Results
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Blocking Assay
http://www.biooncology.com/bioonc/images/her-main.jpg
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Blocking Assay
http://www.biooncology.com/bioonc/images/her-main.jpg
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Blocking Assay
DTEGFATF
http://www.biooncology.com/bioonc/images/her-main.jpg
DTEGFATF
DTEGFATF
DTEGFATF DTEGFATF DTEGFATFDTEGFATF
DTEGFATF
DTEGFATF
DTEGFATF
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Blocking Assay
http://www.biooncology.com/bioonc/images/her-main.jpg
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Blocking Assay Results
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Blocking Assay Results
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Limitations
• Blocking assay– Bispecific cytotoxin disperses throughout tumors– Concentration of antibodies
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Future Work
• Clinical trial
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Acknowledgements• Dr. Daniel Vallera• The University of Minnesota• Vallera Lab• Lois Fruen• Advanced Science Research
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Effectiveness of Bispecific Cytotoxin (DTEGFATF) in Ameliorating Human Glioblastoma Tumors
Travis Spangler2008-2009