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visualizing Gel Electrophoresis
flatbed scanner+
UV transilluminator
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gel stained with Biotium GelRed
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LED light guide (FTIR?)
linear CCD array
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LED light guide (FTIR?)
linear CCD array
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linear CCD array, upside down
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RGB LED (3 diodes)
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RGB LED couples here
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red solid state laser light through light guide rail
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welding curtain: UV filter
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fitting UV curtain over
CCD array
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FOTODYNE UVB transilluminator (circa 1994)
glass (UV-opaque)
special UV-transparent polycarbonate
stained gel
UV-glass(visible-opaque)
transilluminator
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glass (UV-opaque)below gel
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welding curtain on top of gel
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365nm handheld lamp
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exposure: 1/4 sec
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exposure: 5/8 sec
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exposure: 1 sec
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exposure: 2 sec
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exposure: 4 sec
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exposure: 8 sec
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contrast enhanced
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365nm handheld lamp
~365 nm
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visualized w/ ambient light
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visualized w/ ambient light
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visualized w/ UV light from fotodyne
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imaged w/ Lumix GF1 SLR cameraimaged w/ modifed scanner
darklight!
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using small UVB lamp inside the scanner
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next steps:add low-cost filter to linear CCD
GelGreen:Excitation Peak: ~500 nmEmission Peak: ~540 nm
Need to filter emission light before linear CCD- find a filter just above the light source but below the emission- 470-510 nm < filter < 540 nm- TAPR50 sheets filter everything below 528 nm
get TAPR50 528nm filter sheets($35 / 10 sq feet)
http://bit.ly/TAPR50