![Page 1: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/1.jpg)
Genomes and Evolution
Shinichi Morishita
The University of Tokyo
Graduate School of Frontier Sciences,
Department of Computational Biology
Faculty of Science, Bioinformatics & Systems Biology
The figures, photos and moving images with ‡marks attached belong to their copyright holders. Reusing or reproducing them is prohibited unless permission is obtained directly from such copyright holders.
![Page 2: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/2.jpg)
Evolution at Different Levels
• Nucleotide substitution, insertion & deletionDarwin's theory of natural selectionKimura's neutral theory
• Region insertion & deletionTransposons
• Whole-genome duplicationChromosome reorganization
• Ex. HOX genesTranscription factors that induce segmental structure
• Four HOX clusters present in mammals
Figure removed due tocopyright restrictions
Molecular Biology of the Cell - Fifth Edition
Garland Science (2008)
Figure 22-46
![Page 3: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/3.jpg)
GACAATGCAAGAATGAACTCCTTCCTGGAATACCC---CATA
GACAATGCAAGAATGAACTCCTTCCTGGAATACCC---CATA
GACAATGCAAGAATGAACTCCTTTCTGGAATACCC---CATC
GACAATGCAAGAATGAACTCCTTTCTGGAATACCC---CATC
GACAATGCAAGAATGAGCTCCTTCCTGGAATACCC---CATC
GACAATACTAGGATGAACTCCTTCTTAGAGTATGC---AATT
-ACAATGCCACAATGAGCAGCTTCTTAGATTACTC---TGTG
GAAGATGACACAATGAGCACATTCTTAGATTTTTCGTCCATA
XXXXXXXXXXXXMetAsnSerPheLeuGluTyrProXXXIle
XXXXXXXXXXXXATGAACTCCTTCCTGGAATACCCCXXXATA
XXXXXXXXXXXXXXXXXXAGCTTTTTAGAGTATXXXXXXATC
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXATT
XXXXXXXXXXXXXXXSerThrXXXXXXAspPheXXXXXXVal
XXXXXXXXXXXXXXXAGCACAXXXXXXGATTTTXXXXXXGTG
Human
Chimpanzee
Mouse
Rat
Dog
Chicken
Green spotted puffer
Zebrafish
Synonymous
substitution
Non-synonymous
substitution
In mRNA,T becomes U
Harmful mutations are eliminated by natural selection. (Darwin's theory of natural selection, 1859)
DNA retains not only mutations advantageous to survival. Rather, neutral mutations with no effect are predominant. (Motoo Kimura's neutral theory, 1968)
HOX A1
Translation
Kasahara and Morishita , 2006, Large-scale Genome Sequence Processing, Imperial College Press, p.246.
‡
![Page 4: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/4.jpg)
GACAATGCAAGAATGAACTCCTTCCTGGAATACCC---CATA
GACAATGCAAGAATGAACTCCTTCCTGGAATACCC---CATA
GACAATGCAAGAATGAACTCCTTTCTGGAATACCC---CATC
GACAATGCAAGAATGAACTCCTTTCTGGAATACCC---CATC
GACAATGCAAGAATGAGCTCCTTCCTGGAATACCC---CATC
GACAATACTAGGATGAACTCCTTCTTAGAGTATGC---AATT
-ACAATGCCACAATGAGCAGCTTCTTAGATTACTC---TGTG
GAAGATGACACAATGAGCACATTCTTAGATTTTTCGTCCATA
XXXXXXXXXXXXMetAsnSerPheLeuGluTyrProXXXIle
XXXXXXXXXXXXATGAACTCCTTCCTGGAATACCCCXXXATA
XXXXXXXXXXXXXXXXXXAGCTTTTTAGAGTATXXXXXXATC
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXATT
XXXXXXXXXXXXXXXSerThrXXXXXXAspPheXXXXXXVal
XXXXXXXXXXXXXXXAGCACAXXXXXXGATTTTXXXXXXGTG
同義的置換
非同義的置換
mRNA中はT は U
GACAATGCAAGAATGAACTCCTTCCTGGAATACCC---CATA
||xx|||xx|x|||||x|x|x|||x|x||x|xxx| ||||
GAAGATGACACAATGAGCACATTCTTAGATTTTTCGTCCATA
Human
Zebrafish
Blowfish
Zebrafish
-ACAATGCCACAATGAGCAGCTTCTTAGATTACTC---TGTG
|xx|||x|||||||||||xx||||||||||xx|| xx|x
GAAGATGACACAATGAGCACATTCTTAGATTTTTCGTCCATA
HOX A1
Kasahara and Morishita , 2006, Large-scale Genome Sequence Processing, Imperial College Press, p.246‡
Human
Chimpanzee
Mouse
Rat
Dog
Chicken
Green spotted puffer
Zebrafish
Translation
![Page 5: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/5.jpg)
The Fossil Record and Molecular ClocksPauling and Zuckerkandl, 1962
Studied the number of substitutions in the 141 amino acids in hemoglobin α chains.
Fossil record indicates strong correlation between time of divergence and rate of substitution.
Suggested that the rate of substitution is more or less uniform
==> Calculation of date of divergence from rate of substitution
Our current understanding is that:
The molecular clock is not necessarily accurate.
Because the mutation rate varies with lineage and is not uniform.
Takashi Miyata, Shinka no Hanashi ("On Evolution"), JT Biohistory Research Hallhttp://www.brh.co.jp/katari/shinka/shinka09.html
Gorilla Horse Dog Newt Carp
Human 1 18 23 62 68
‡
Teleosts/selachians
Am
ino a
cid s
ubst
itutions
(per
posi
tion)
Date of divergence (million years)
Teleosts/quadrupeds
Amphibians/amniota
Mammals/birds
Marsupials/placentals
Primates/rodents
![Page 6: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/6.jpg)
Using Mitochondrial DNA
• Almost entirely matrilineal, making it easy to trace ancestry. (Some patrilineal.)
• c.16,500 base pairs in all
• Human and chimpanzee (diverged c.5 million years ago)
Differences in mitochondrial DNA genes of about 9%
(in nuclear DNA, about 1%)
Authoritative calculation of date of divergence in tens of thousands of years
• Alan Wilson, 1987
Produced a genealogical tree from the mitochondria of 147 persons.
Our ancestor is an African woman from 150,000 to 290,000 years ago (the Mitochondrial Eve hypothesis).
• 1991: 5,000-year-old mummy found in the Alps (the "Iceman").
![Page 7: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/7.jpg)
Chromatin Structure, Transcription and Evolution
Figures from Seimei Kagaku ("Life Sciences"), 2nd rev. ed., Yodosha, 2008, Figures 4-6, 7, 8
‡
Histone proteins
Assembly
Octamerichistone core
DNA double helix
Nucleosome structure
Transcriptionfactor
Transcriptionfactor
HistoneAcetylase
HistoneAcetylase
Nucleosomesuperhelix
Full metaphasechromosome
Promoter
Histone H3
Histone H4
Histone H2A
Histone H2B
: Lysine: Arginine: Serine
: Methylation: Acetylation: Phosphorylation: Ubiquitination
DNA Methylation and Epigenetics
Histone methylase
Methylated DNA
Methylated DNA bindingProtein complex
Methylated histone
Methylated DNA
Methylated histoneBinding protein
Heterochromatin formation
Transcription inactivation
Epigenetic regulation of expression
![Page 8: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/8.jpg)
Occurrence of nucleosome cores in killifish genome
Transcription start site
Chart from Sasaki et al., 2009, Science, 323(5912), 401-404
‡
Transcriptionfactor
Chromatin Structure, Transcription and Evolution
![Page 9: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/9.jpg)
Base substitution rate
Insertion/deletion rate
Chart from Sasaki et al., 2009, Science, 323(5912), 401-404
Chromatin Structure, Transcription and Evolution
Genomes of two killifish varieties compared
‡
Transcription start site
Transcriptionfactor
![Page 10: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/10.jpg)
millions of years ago
Sarcopterygii
Actinopterygii
Osteichthyes
Verterata
Chondrichthyes
Urochordata
Cephalochordata
Gnathostomata
Teleostei
Chordata
Polypteriformes
Holostei
Lancelet
Sea squirt
Lamprey
Shark, ray
Sturgeon, gar, bowfin
Zebrafish
Killifish
Green spotted puffer
Tiger blowfish
Coelacanth, lungfish
Agnatha
Dipnoi
Bichir
Evolution by Duplication
Susumu Ohno
Evolution by Gene Duplication1970
Whole-genome
duplication
Whole-genome duplication
500 400 300 200 100 0
Enrichment of genesNeo-functionalizationSub-functionalizationMostly functionlessdue to mutations
‡
‡ Nakatani et al., 2007 , Genome Res., 17, 1254-1265
Mesozoic CenozoicPaleozoic
Cambrian TriassicJurassic
CretaceousPaleogene
NeogeneCarboniferousPermianDevonian
Silurian
Ordovician
CrocodileTetrapoda
Amniota
AmphibiaFrog
Reptilia
Aves
Squamata
Crocodilia
Testudinata
Lizard, snake
Chicken
Turtle
Mammalia
Dog
Mouse, rat
Human, chimpanzee
Whole-genome
duplication
![Page 11: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/11.jpg)
millions of years ago
Sarcopterygii
Actinopterygii
Osteichthyes
Verterata
Chondrichthyes
Urochordata
Cephalochordata
Gnathostomata
Teleostei
Chordata
Polypteriformes
Holostei
Lancelet
Sea squirt
Lamprey
Shark, ray
Sturgeon, gar, bowfin
Zebrafish
Killifish
Green spotted puffer
Tiger blowfish
Coelacanth, lungfish
Agnatha
Dipnoi
Bichir
Evolution by Duplication
Susumu Ohno
Evolution by Gene Duplication1970
Whole-genome
duplication
Whole-genome duplication
500 400 300 200 100 0
Enrichment of genesNeo-functionalizationSub-functionalizationMostly functionlessdue to mutations
‡
‡
Mesozoic CenozoicPaleozoic
Cambrian TriassicJurassic
CretaceousPaleogene
NeogeneCarboniferousPermianDevonian
Silurian
Ordovician
CrocodileTetrapoda
Amniota
AmphibiaFrog
Reptilia
Aves
Squamata
Crocodilia
Testudinata
Lizard, snake
Chicken
Turtle
Mammalia
Dog
Mouse, rat
Human, chimpanzee
Whole-genome
duplication
Nakatani et al., 2007 , Genome Res., 17, 1254-1265
Figure removed due to
copyright restrictions
Molecular Biology of the Cell - Fifth Edition
Garland Science (2008)
Figure 22-46
![Page 12: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/12.jpg)
Inferences from Hox Genes (1997) Human
Frog
Shark
Zebrafish
Killifish
Green spotted puffer
Bichir
Actinopterygii
Osteichthyes
Mammalia
Verterata
Chondrichthyes
Amphibia
Gnathostomata
Teleostei
Polypteriformes
Sarcopterygii
Sea squirts have a single Hox gene cluster
Coelacanth
Chart fromHoegg, Meyer , 2005, Trends Genet. , 21(8), 421-4
A
B
C
D
A
A
B
B
C
C
D
D b
b
b
b
a
a
a
a
D
C
B
A
C
B
A
D
D
C
B
A
D
C
B
A
D b
C b
D a
B b
C a
A b
B a
A a
D b
C b
D a
B b
C a
A b
B a
A a
ゼブラフィッシュ
D b
C b
D a
C a
B b
B a
A b
A a
A
B
C
D ?
?
?
D
C
B
A
Whole-genome
duplication
Whole-genome
duplicationWhole-genome
duplication
![Page 13: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/13.jpg)
Can Whole-Genome Duplication Be Confirmed?
• The human genome has up to 300,000 base pairs where Hox genes have clustered.
• This represents a tiny fraction of the entire human genome (around three billion base pairs).
• Compare entire genomes.
![Page 14: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/14.jpg)
Traces of Two Whole-Genome Duplications Detected in Vertebrate Lineage
71712
2 Human chromosome 7
The chart plots chromosomal paralogue (genes with similar sequences) found of genes in human chromosome 7.
C
B
A
D
Figure removed due tocopyright restrictions
Nakatani et al., 2007 , Genome Res., 17, 1254-1265
supplemental research data
![Page 15: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/15.jpg)
7
1712
2
1116
Hu
ma
n C
hro
mo
so
me
Killifis
h C
hro
mo
so
me
ヒト7番染色体
出典 Nakatani Y et al. Genome Res , 2007, 17(9): 1254-1265
Figure removed due tocopyright restrictions
Nakatani et al., 2007 , Genome Res., 17,
1254-1265
supplemental research data
Figure removed due tocopyright restrictions
Nakatani et al., 2007 , Genome Res., 17,
1254-1265
supplemental research data
The chart plots killifish orthologues (genes with similar sequences) found of genes in human chromosome 7.
![Page 16: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/16.jpg)
Sarcopterygii
Actinopterygii
Osteichthyes
Verterata
Chondrichthyes
Urochordata
Cephalochordata
Gnathostomata
Teleostei
Chordata
Polypteriformes
Holostei
Lancelet
Sea squirt
Lamprey
Shark, ray
Sturgeon, gar, bowfin
Zebrafish
Killifish
Green spotted puffer
Tiger blowfish
Coelacanth, lungfish
Agnatha
Dipnoi
Bichir
Whole-genome
duplication
Whole-genome duplication
CrocodileTetrapoda
Amniota
AmphibiaFrog
Reptilia
Aves
Squamata
Crocodilia
Testudinata
Lizard, snake
Chicken
Turtle
Mammalia
Dog
Mouse, rat
Human, chimpanzee
Whole-genome
duplication
Figure removed due to
copyright restrictions
Nakatani et al., 2007,
Genome Res., 17, 1254-1265
Figure4
Gnathostomata(40)
Osteichthyes (31)
Amniota (26)
Kasaahra M et al. Nature 447, 714-719 (2007)Nakatani Y et al. Genome Res 17(9), 1254-1265 (2007).
Verterata (10)
![Page 17: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/17.jpg)
Sarcopterygii
Actinopterygii
Osteichthyes
Verterata
Chondrichthyes
Urochordata
Cephalochordata
Gnathostomata
Teleostei
Chordata
Polypteriformes
Holostei
Lancelet
Sea squirt
Lamprey
Shark, ray
Sturgeon, gar, bowfin
Zebrafish
Killifish
Green spotted puffer
Tiger blowfish
Coelacanth, lungfish
Agnatha
Dipnoi
Bichir
Whole-genome
duplication
Whole-genome duplication
CrocodileTetrapoda
Amniota
AmphibiaFrog
Reptilia
Aves
Squamata
Crocodilia
Testudinata
Lizard, snake
Chicken
Turtle
Mammalia
Dog
Mouse, rat
Human, chimpanzee
Whole-genome
duplication
Figure removed due to
copyright restrictions
Nakatani et al., 2007,
Genome Res., 17, 1254-1265
Figure4
Gnathostomata(40)
Osteichthyes (31)
Amniota (26)
Kasaahra M et al. Nature 447, 714-719 (2007)Nakatani Y et al. Genome Res 17(9), 1254-1265 (2007).
Verterata (10)
Teleostei (13)
![Page 18: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/18.jpg)
(小鹿)Figure removed due to copyright restrictions
Molecular Biology of the Cell - Fifth Edition
Garland Science (2008)
Figure 4-14
![Page 19: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/19.jpg)
Global Focus on Knowledge Lecture Series Puzzling Out the System of Life
Genomes and Evolution:The Path to Humanity
Naruya SaitouNational Institute of Genetics
April 22, Year 9 of the Scientific EraBldg 18 Lecture Hall, Komaba Campus, The University of Tokyo
The figures, photos and moving images with ‡marks attached belong to their copyright holders. Reusing or reproducing them is prohibited unless permission is obtained directly from such copyright holders.
![Page 20: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/20.jpg)
Charles Darwin (1809-1882)
![Page 21: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/21.jpg)
A History of Life and the Universe, in Three Volumes
‡Naruya Saitou, E de Wakaru Jinrui no Shinka(Evolution of the Human Race which is Understood with Pictures), Koudan Sha, 2009. Illustrations: Saori Yasutomi
![Page 22: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/22.jpg)
‡
Lord Kelvin
"It took at most 100 million years for the Earth to cool down." Alpha rays Beta rays
Uranium 238 Protactinium 234Thorium 234
Pierre and Marie Curie
"Given the energy emitted from radioisotopes, it's taken longer than that . . ."
Naruya Saitou, E de Wakaru Jinrui no Shinka(Evolution of the Human Race which is Understood with Pictures), Koudan Sha, 2009. Illustrations: Saori Yasutomi
![Page 23: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/23.jpg)
200 Million Years of Continental Drift
‡
Laurasia
Gondwana
Naruya Saitou, E de Wakaru Jinrui no Shinka(Evolution of the Human Race which is Understood with Pictures), Koudan Sha, 2009.
Illustrations: Saori Yasutomi
![Page 24: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/24.jpg)
Australia
Africa
Laurasia
Gondwana‡
イラスト:安富佐織
Mammalian Genealogy(Past 100 million years)
Un
gu
icu
late
sLa
ura
sia
n a
nim
als
LaurasiaA
frica
n a
nim
als
Ed
en
tate
s
South America
Australia
Naruya Saitou, E de Wakaru Jinrui no Shinka(Evolution of the Human Race which is Understood with Pictures), Koudan Sha, 2009.
![Page 25: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/25.jpg)
Primate Genealogy(Past 50 million years)
‡
HumanChimpanzeeGorillaOrangutanGuenonJapanese macaqueTaiwan macaqueMacaca mulattaCrab-eating macaqueHamadryas baboon
Olive baboonGeladaMandrillPatas monkeyColobusLangurSpider monkeyWooly monkeyHowler monkeyCapuchin
Squirrel monkeyNight monkeyTarsierLemur
Aye-ayeIndriLoris
Anth
ropoid
sO
ld W
orld
monke
ys
New
World
monkeys
Pro
simia
ns
Maca
ques
Baboons
Time of divergence (10 mn years ago)
Naruya Saitou, Genomu to Shinka – Genomu kara Tachinoboru Seimei (Genomes and Evolution –Life Ascendent from Genomes), Shin'yosha, 2004.
![Page 26: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/26.jpg)
Hominoid Genealogy(Past 20 million years)
Hominidae
Hylobatidae
Time of divergence (10 mn years ago)
‡Naruya Saitou, Genomu to Shinka – Genomu kara Tachinoboru Seimei (Genomes and Evolution –Life Ascendent from Genomes), Shin'yosha, 2004.
![Page 27: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/27.jpg)
Mutation
The Heart of Evolution:DNA accumulates mutations while undergoing repeated self-replication.
![Page 28: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/28.jpg)
塩基の置換 もとの塩基配列:ACCTATTTTGCTG 新しい塩基配列:ACCTGTTTTGCTG 塩基の挿入 もとの塩基配列:ACCTATTTTGCTG 新しい塩基配列:ACCAGTTATTTTGCTG 塩基の欠失 もとの塩基配列:ACCTATTTTGCTG 新しい塩基配列:ACCTATTGCTG
微小な突然変異のいろいろDifferent Micromutations
Nucleotide replacement
Nucleotide insertion
Nucleotide deletion
Original sequence:
New sequence:
Original sequence:
New sequence:
Original sequence:
New sequence:
![Page 29: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/29.jpg)
Mutations Accumulating in DNA
DNA of ancestral life form
DNA of current life forms
![Page 30: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/30.jpg)
![Page 31: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/31.jpg)
![Page 32: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/32.jpg)
Raphael's Scuola di Atene, detail
Left: PlatoRight: Aristotle
Matter and Essence
![Page 33: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/33.jpg)
What Is a Genome?
• A genome is the sum of its genes and its chromosomes. (Hans Winkler, 1920)
• Functional definition: The minimal set of genes required for the life of an organism. (Hitoshi Kihara, 1930s)
• Structural definition: The largest self-replicating unit of nucleotide sequences. (Naruya Saitou, 2004)
![Page 34: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/34.jpg)
Human Chromosomes
Human
Cellular nucleus
![Page 35: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/35.jpg)
ABO blood group gene
Human genome: 3 billion nucleotides
22 autosomal chromosomes and2 sex chromosomes
22,000 to 50,000 genes
![Page 36: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/36.jpg)
From Chromosome to Nucleotide Sequence:The ABO Blood Type Gene
‡
Chromosome 9
ABO blood type gene
Exon 7
Exon 7
Exon 1
Intron 6 Naruya Saitou, Genomu ha Koko made Kaimei Sareta(What We Know of Genomes), Wedge, 2007.
![Page 37: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/37.jpg)
Splicing (Exon Patching)
‡
Exon Intron
Naruya Saitou, E de Wakaru Jinrui no Shinka(Evolution of the Human Race which is Understood with Pictures), Koudan Sha, 2009. Illustrations: Saori Yasutomi
A C B ODZ E Z WYY O MRD
COD E WORD
![Page 38: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/38.jpg)
Direction of Flow of Genetic Information(The central drama of molecular biology)
Pro
tein
TranslationTranscription
Reversetranscription
![Page 39: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/39.jpg)
3D Structure of ABOBlood Type Enzymes(Patenaude et al, 2002)
Reprinted by permission from Macmillan Publishers Ltd:Sonia I. Patenaude1,et al.,Nature Structural Biology 9, 685 – 690, copyright (2002)
‡
![Page 40: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/40.jpg)
Structure of ABO Blood Type AntigensThe ABO gene produces glycosyltransferase
N-acetyl galactosamine (w/ tail) to cell surface
to cell surface
Enzyme AType O antigen
Enzyme B
Galactose(no tail)
Sugar chain attached to cell-surface protein
Type A antigen
Type B antigen
![Page 41: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/41.jpg)
O遺伝子における1塩基の欠失 (X ) によって引き起こされたフレームシフト突然変異
塩基配列: A AAGGATGTCCTCGTGGTGACCCCTTGGCTGGCTCCCATTGTCTGGGAGGGCACATTCAAC
B G
O X G
タンパク質のアミノ酸配列: A/B K D V L V V T P W L A P I V W E G T F N
O K D V L V V P L G W L P L S G R A H S T A転移酵素と B 転移酵素 の酵素活性の違いを与えている2個のアミノ酸の違い(+)と、そ
れを引き起こすA遺伝子とB遺伝子2個の塩基の違い (!)
遺伝子の塩基配列 :
A GATTTCTACTACCTGGGGGGGTTCTTCGGGGGGTCGGTGCAAGAG B A C
! !
酵素タンパク質のアミノ酸配列 :
A D F Y Y L G G F F G G S V Q R B D F Y Y M G A F F G G S V Q R
+ +
(山本文一郎ら、Nature, 1990年)
Frame-shift mutation caused by the deletion of one nucleotide (X) in the O allele
Nucleotide sequences:
Protein amino acid sequences:
The differences (+) in two amino acids conferring a difference in enzyme activity between transferase A and transferase B, and the differences(!) between two nucleotides in gene A and gene B causing that to happen
Genetic nucleotide sequences:
Enzyme protein amino acid sequences:
(Fumiichiro Yamamoto et al., Nature, 1990)
![Page 42: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/42.jpg)
Synonymous and Non-synonymous Substitutionin Protein Translation Regions
Synonymous substitution:Nucleotide substitution (neutral only)that does not modify amino acids
Non-synonymous substitution:Nucleotide substitution (neutral and selective)that modifies amino acids
![Page 43: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/43.jpg)
Dry (function-less)resulted frommoist (functional)with modification of a single amino acid
Moist protein (glycine)
Dry protein (arginine)
No protein
Differences in the Function of ABCC11 Gene ProductAre the Cause of the Differences in Earwax
Yoshiura et al (2006)
Yoshiura et al (2006)
‡ Reprinted by permission from Macmillan Publishers Ltd, Nature Genetics 38, 324 - 330, copyright (2006)
![Page 44: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/44.jpg)
Non-synonymous substitution
Mutation rate=Evolutionary rate of purely neutral evolution
Mutation disappears
Evolutionary Rates of Synonymous and Non-synonymous Substitution
Synonymous substitution
Positiveselection
Purifyingselection
![Page 45: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/45.jpg)
Synonymous substitutionN
on
-syn
on
ym
ou
s s
ub
stitu
tion
Non-synonymoussubstitution =Synonymous substitution
Synonymous/Non-synonymous Substitution Ratioin Comparison of Human and Mouse Genes
Saitou, Genomu Shinka-gaku Nyuumon(Introduction to Genome Evolution), 2007
‡ Naruya Saitou, Genomu Shinka Gaku Nyuumon(Introduction to Genome Evolution), Kyouritsu Shuppan, 2007.
![Page 46: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/46.jpg)
International Human Genome Sequencing Consortium (2004)
Entire human genome (3.2 billion nucleotides) ┃┣Genes and gene-related regions (1.2 billion nucleotides)┃ ┃┃ ┣Genes (48 million nucleotides) <-- Just 1.5% of the whole
┃ ┃┃ ┗Gene-related regions (1,152 million nucleotides)┃┗Regions between genes (2.0 billion nucleotides)
┃┣Dispersed repetitive sequences (1.4 billion nucleotides)┃ ┃┃ ┣Long dispersed repetitive sequences LINE (640 million nucleotides)┃ ┃┃ ┣Short dispersed repetitive sequences SINE (420 million nucleotides)┃ ┃┃ ┗Other repetitive sequences SINE (340 million nucleotides)┃┗Other intergenic regions (600 million nucleotides)
┃┣Microsatellites (90 million nucleotides)┃┗Non-microsatellites (510 million nucleotides)
![Page 47: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/47.jpg)
International Human Genome Sequencing Consortium (2004)
Entire human genome (3.2 billion nucleotides) Almost entirely junk DNA┃┣Genes and gene-related regions (1.2 billion nucleotides)┃ ┃ Almost entirely junk DNA┃ ┣Genes (48 million nucleotides) <-- Just 1.5% of the whole
┃ ┃┃ ┗Gene-related regions (1,152 million nucleotides)┃ Almost entirely junk DNA┗Regions between genes (2.0 billion nucleotides)
┃ Almost entirely junk DNA┣Dispersed repetitive sequences (1.4 billion nucleotides)┃ ┃ Almost entirely junk DNA┃ ┣Long dispersed repetitive sequences LINE (640 million nucleotides)┃ ┃ Almost entirely junk DNA┃ ┣Short dispersed repetitive sequences SINE (420 million nucleotides)┃ ┃ Almost entirely junk DNA┃ ┗Other repetitive sequences SINE (340 million nucleotides)┃ Almost entirely junk DNA┗Other intergenic regions (600 million nucleotides)
┃ Almost entirely junk DNA┣Microsatellites (90 million nucleotides)┃ Almost entirely junk DNA┗Non-microsatellites (510 million nucleotides)
Almost entirely junk DNA
![Page 48: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/48.jpg)
Mouse–Rat Differences in Intergenic Regionsand in Synonymous Sites-------------------------------------------------Regions Sites NucleotideCompared compared Substitutions
-------------------------------------------------Intergenic regions 27,931,288 0.15 〜 0.16Synonymous sites 955,105 0.16 〜 0.17-------------------------------------------------Naruya Saitou, Genomu Shinka Gaku Nyuumon(Introduction to Genome Evolution), 2007.Source data from Abe et al (2004)
The bulk of our genome is the product of neutral evolution!
![Page 49: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/49.jpg)
Prof. Motoo Kimura and his paper outlining neutral evolution theory (1968)
![Page 50: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/50.jpg)
How the Neutral and Natural Selection Theories Differ
All mutations
Mutationscontributingto evolution
Mutations notcontributingto evolution
Mutationsadvantageous to survival
Neutralmutations
Neutralevolutiontheory
Theory ofnaturalselection
![Page 51: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/51.jpg)
MutationHold
"Of all these mutations, just this one box has ones that contribute to evolution."
What does the box hold?
Neutral Evolution Theory
"Just a few are advantageous to
survival. The others are
neutral."
‡
Theory of Natural Selection
Evolutionarycontribution
"No, no! They all contribute to survival."
Evolutionarycontribution
Naruya Saitou, E de Wakaru Jinrui no Shinka(Evolution of the Human Race which is Understood with Pictures), Koudan Sha, 2009.
Illustrations: Saori Yasutomi
![Page 52: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/52.jpg)
Genes That Evolved Non-neutrally
• mmunological system genes
• Viral genes that attack host immunological systems
• Some cell-surface molecules (e.g. blood type)
• Genes for plant systems that prevent self-fertilization
• Surface proteins in marine animal sperm (differentiation from other species)
• Causative protein in snake venom
![Page 53: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/53.jpg)
Neutral
DNA level
Neutral
Protein level
True natural selection
Macro phenotype level
Proportion of Neutral Mutations at Three Levels as Reckoned by Many Researchers
![Page 54: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/54.jpg)
True natural selection
Proportion of Neutral Mutations at Three Levels as Reckoned by Me
DNA level
Protein level
Macro phenotype level
Neutral
Neutral
Neutral
![Page 55: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/55.jpg)
Comparative Analysis of Human and Anthropoid Genomes
Copyright © 2008 Conor Keller Copyright © 2005 belgianchocolate Copyright © 2006 mape_s Copyright © 2008 B&M Photography‡ ‡ ‡ ‡
![Page 56: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/56.jpg)
Kuroki et al (2006)
‡
Reprinted by permission from Macmillan Publishers Ltd: Nature Genetics 38, 158 - 167, copyright (2006)
![Page 57: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/57.jpg)
Pedigree of a family exhibiting language disorder(Lai et al., 2001)
‡ Reprinted by permission from Macmillan Publishers Ltd: Nature, 413, 519-523, copyright (2001)
![Page 58: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/58.jpg)
A Language Gene? The Structure of the FOXP2 Gene
Approximate location in human genome:Human chromosome 7, on the long arm (q)
Lai et al,.(2001)‡
Reprinted by permission from Macmillan Publishers Ltd: Nature, 413, 519-523, copyright (2001)
![Page 59: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/59.jpg)
‡ Illustrations: Saori Yasutomi
Human
Chimpanzee
Gorilla
Orangutan
Macaca mulatta
Mouse
Did Changes in Amino Acids of the FOXP2 Protein Contribute to Capacity for Language?
![Page 60: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/60.jpg)
Reverse-Nested Structure of the Human Genome
Human Genome
Bacteria
Eukaryotes
Vertebrates
Primates
Hominoids
Mammals
![Page 61: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/61.jpg)
Bacteria
Bacteria
‡
Reverse-Nested Structure of the Human Genome
![Page 62: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/62.jpg)
Bacteria
Eukaryotes Eukaryotes
Copyright © 2006 Masur
‡
Reverse-Nested Structure of the Human Genome
![Page 63: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/63.jpg)
Bacteria
Eukaryotes
Vertebrates
Vertebrates
Copyright © 2007 DDFic
‡
Reverse-Nested Structure of the Human Genome
![Page 64: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/64.jpg)
Bacteria
Eukaryotes
Vertebrates
Mammals
Mammals
Copyright © 2006 GirlReporter‡
Reverse-Nested Structure of the Human Genome
![Page 65: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/65.jpg)
Bacteria
Eukaryotes
Vertebrates
Primates
Primates
Mammals
Copyright © 2007 GATAG‡
Reverse-Nested Structure of the Human Genome
![Page 66: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/66.jpg)
Bacteria
Eukaryotes
Vertebrates
Primates
Hominoids
Hominoids
Mammals
Copyright © 2005 belgianchocolate‡
Reverse-Nested Structure of the Human Genome
![Page 67: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/67.jpg)
Human Genome
Bacteria
Eukaryotes
Vertebrates
Primates
Hominoids
Hominoids
Mammals
Copyright © 2005 belgianchocolate‡
Reverse-Nested Structure of the Human Genome
![Page 68: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/68.jpg)
FOXP2
DNA Regions Considered Evolutionarily Significant in All Mammals
![Page 69: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/69.jpg)
Humans and Other Life Forms
Copyright © 2005 belgianchocolate Copyright © 2006 mape_s Copyright © 2008 B&M Photography‡ ‡ ‡
![Page 70: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/70.jpg)
Humans and Chimpanzees, and the Rest?
Copyright © 2005 belgianchocolate Copyright © 2006 mape_s Copyright © 2008 B&M Photography‡ ‡ ‡
![Page 71: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/71.jpg)
Humans and Large Anthropoids, and the Rest?
Copyright © 2005 belgianchocolate Copyright © 2006 mape_s Copyright © 2008 B&M Photography‡ ‡ ‡
![Page 72: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/72.jpg)
Shin'yosha (2004)
‡
Genomes and Evolution:Life Ascendent from Genomes
by Naruya Saitou
Naruya Saitou, Genomu to Shinka – Genomu kara Tachinoboru Seimei (Genomes and Evolution –Life Ascendent from Genomes), Shin'yosha, 2004.
![Page 73: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/73.jpg)
Published March 2006
The Iwanami Shoten Evolution Series Vol. 2
Idenshi to Genomu no Shinka(Evolution of Genes and Genomes)
Hajime Ishikawa, Noriyuki Sato, Mariko Hasegawa and Naruya Saitou, eds.
Introduction by Naruya Saitou
Chapter 1 The Mechanism of Genetic Evolution, by Naruya Saitou
Chapter 2 The Evolution of Proteins, by Hiroyuki Toh
Chapter 3 The Evolution of Bacterial Genomes, by Ichizo Kobayashi
Chapter 4 The Evolution of Multicellular Organisms, by Takeshi Kawashima and Noriyuki Sato
Chapter 5 Ancient DNA, by Shintaro Ueda and Naruya Saitou
Chapter 6 The Role of Bioinformatics, by Takashi Gojobori
![Page 74: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/74.jpg)
Published March 2007Naruya Saitou, ed.
Genomu ha Koko made Kaimei Sareta(What We Know of Genomes)
(Wedge Sensho)• “An Integrated Understanding of Matter and Life as Disclosed by
Genomes,” by Akiyoshi Wada
• “Decoding the Human Genome, and Metagenomes,” by Yoshiyuki Sakaki
• “Genomes and Animal Body Formation, and Gene Networks,” by Noriyuki Sato
• “Tracing to Humans the History of Life Written in Genome Sequences,” by Naruya Saitou
• A Genomics Roundtable: "From microorganisms to Neanderthals" (Asao Fujiyama, Shohei Hattori, Shin'ichi Morishita, Naruya Saitou)
![Page 75: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/75.jpg)
Introduction to Genome Evolution,by Naruya SaitouKyouritsu Shuppan(2007)
‡
![Page 76: Genomes and Evolution - UTokyo OpenCourseWare · 2011. 5. 31. · Gorilla Horse Dog Newt Carp Human 1 18 23 62 68 ‡ Teleosts/selachians on) Date of divergence (million years) Teleosts/quadrupeds](https://reader036.vdocuments.net/reader036/viewer/2022071404/60f9158732e4cf1d3a2b60ab/html5/thumbnails/76.jpg)
Up Next on Friday, May 1:"What Our Genome Says about the
Origins of the Japanese"
Naruya Saitou
DNA kara Mita Nihonjin
(The Japanese in Terms of DNA)
Chikuma Shinsho, 2005
‡