![Page 1: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/1.jpg)
2015 LanzaTech. All rights reserved.
1
GMOD powering LanzaTech
2016 GMOD Meeting
Indiana University, Bloomington, Indiana, United States
June 30 – July 1, 2016
Asela W. S. Dassanayake
Bioinformatics Research Scientist
Model Systems Group
![Page 2: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/2.jpg)
2
• LanzaTech - Who we are and what we do
• How GMOD is powering the information flow at LanzaTech
• Proposing a Synthetic Biology platform?
The outline
![Page 3: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/3.jpg)
3
Ed Hawkins,Climate scientist,National Centre for Atmospheric Science,University of Reading.@ed_hawkins
![Page 4: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/4.jpg)
65% of 2°carbon budget: USED
1870-2011: 1900 GtCO2
Remaining: 1000 GtCO2
Must stay in the ground
4
Carbon smart world
![Page 5: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/5.jpg)
Proprietary Acetogenic Biocatalyst
5
Worlds’ first autotrophs
Acetogenic bacterium with ability to utilize gases as sole energy and carbon source – CO– CO+H2 or CO+CO2+H2
– CO2+H2
LanzaTech has developed a proprietary
strain of Clostridium autoethanogenum
Obtained by extensive selection program,
having improved characteristics– High gas uptake and ethanol production rates– Fast growth on defined minimal media– Non-sporulating and non-motile
1
![Page 6: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/6.jpg)
1 Organism, over 20 Products…
Pyruvate
CO/H2
Acetyl-CoA
Fatty Acids,
Terpenoids
Aromatics
Ethanol
Succinate
Lactate
2,3-Butanediol
(2,3-BDO)
Biodiesel (FAEE)
3-Hydroxypropionate
(3-HP)
Isopropanol
Acetone
3-Hydroxybutyrate
(3-HB)
1,3-Butanediol (1,3-BDO)
Partnerships
Butylene
1,3-Butadiene
Biopolymers
Branched-chain
Amino Acids
Butyrate
1-Butanol
Methyl Ethyl Ketone
(MEK)
2-Butanol
Acetoin
1,2-Propanediol
1-Propanol
Jet Fuel
Isoprene
Aromatics
DiscoveryContinuous
lab-scale
fermentation
Scaled
Up1 Organism, over 25 Products…
Acetate
6
Piloting>50% selectivity
at lab scale
![Page 7: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/7.jpg)
Gas Feed Stream
Gas Reception Compression Fermentation Recovery ProductTank
HydrocarbonFuels
Ethanol
Butanediol
Acrylonitrile
Butadiene
Styrene (ABS)
Nylon 6,6 (from Adiponitrile/HDMA)
PropanolButanol
The LanzaTech Process
![Page 8: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/8.jpg)
Industrial Waste Gas
MSW WasteBiomass
We use:
Land Corn For People
We do not use:
8
A sensible path
SugarCane
![Page 9: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/9.jpg)
Strong Pipeline and Committed Partners
Commercial Production 2017
3 Commercial projects underway
![Page 10: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/10.jpg)
ArcelorMittal, Gent
10
Ground Works StartedOctober 2015
Gas Testing Station Produces Ethanol
January 2016
Connection to Steel Mill Gas Lines March 2016
![Page 11: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/11.jpg)
1 Organism, over 20 Products…
Pyruvate
CO/H2
Acetyl-CoA
Fatty Acids,
Terpenoids
Aromatics
Ethanol
Succinate
Lactate
2,3-Butanediol
(2,3-BDO)
Biodiesel (FAEE)
3-Hydroxypropionate
(3-HP)
Isopropanol
Acetone
3-Hydroxybutyrate
(3-HB)
1,3-Butanediol (1,3-BDO)
Partnerships
Butylene
1,3-Butadiene
Biopolymers
Branched-chain
Amino Acids
Butyrate
1-Butanol
Methyl Ethyl Ketone
(MEK)
2-Butanol
Acetoin
1,2-Propanediol
1-Propanol
Jet Fuel
Isoprene
Aromatics
DiscoveryContinuous
lab-scale
fermentation
Scaled
Up1 Organism, over 25 Products…
Acetate
11
Piloting>50% selectivity
at lab scale
![Page 12: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/12.jpg)
12
BIOENGINEERING
FERMENTATION
GENOMIC, TRANSCRIPTOMIC,
PROTEOMIC, METABOLOMIC DATA
BIOINFORMATICS, STATISTICS, MODELING
BIOCHEMISTRY, PATHWAY ANALYSIS
Information flow at LanzaTech
![Page 13: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/13.jpg)
13
GMOD at LanzaTech
External Collaborators
![Page 14: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/14.jpg)
14
Apollo at LanzaTech - Internal
![Page 15: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/15.jpg)
15
Transcriptional Regulator
Methyl accepting chemotaxis protein
Transcriptional Regulator
Uncharacterised ORF
Iron-Sulphur binding cluster
Roshomon
![Page 16: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/16.jpg)
16
Tuff-t
![Page 17: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/17.jpg)
17
Tuff-t
![Page 18: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/18.jpg)
1 Organism, over 20 Products…
Pyruvate
CO/H2
Acetyl-CoA
Fatty Acids,
Terpenoids
Aromatics
Ethanol
Succinate
Lactate
2,3-Butanediol
(2,3-BDO)
Biodiesel (FAEE)
3-Hydroxypropionate
(3-HP)
Isopropanol
Acetone
3-Hydroxybutyrate
(3-HB)
1,3-Butanediol (1,3-BDO)
Partnerships
Butylene
1,3-Butadiene
Biopolymers
Branched-chain
Amino Acids
Butyrate
1-Butanol
Methyl Ethyl Ketone
(MEK)
2-Butanol
Acetoin
1,2-Propanediol
1-Propanol
Jet Fuel
Isoprene
Aromatics
DiscoveryContinuous
lab-scale
fermentation
Scaled
Up1 Organism, over 25 Products…
Acetate
18
Piloting>50% selectivity
at lab scale
![Page 19: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/19.jpg)
19
• User data organization and management• BLAST Interface• Database Searching
• Literature searches and bibliography
• Genome browser
• Data Visualization
• Synthetic Biology CAD with SBML/SBOL
Proposing an Integrated Synthetic Biology platform
![Page 20: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/20.jpg)
20
Integrated Synthetic Biology platform
![Page 21: GMOD powering LanzaTechgmod.org/mediawiki/images/1/15/GMOD_Asela.pdfGMOD powering LanzaTech 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 –July](https://reader031.vdocuments.net/reader031/viewer/2022022521/5b23f1547f8b9ab8268b4a15/html5/thumbnails/21.jpg)
21
More Users? Funding?