Download - High Efficiency Electroporation
FOR TECHNICAL SUPPORT | Tel +1.608.441.2852 | www.TheTransfectionExperts.com
Ingenio® Electroporation Solution & Kits
Save on Cost Without Compromising Your Results Product Cost/Electroporation* Savings
Amaxa® Nucleofector® Kit V (VCA-1003) -
Ingenio® Electroporation Kit (MIR 50112) 42% *Based on list prices from company websites and protocol recommendations (25 RXN Kit).
Figure 1. Cells were electroporated in parallel with an EGFP reporter vector and assayed at 24 hours post-electroporation by flow cytometry. Two electroporators were used with different electroporation solutions: the Ingenio® Electroporation Kit was used in the Gene Pulser Xcell™ Eukaryotic System (Bio-Rad) or the Amaxa Nucleofector II Device (Lonza); the Amaxa Nucleofector Kit V was used in the Amaxa Nucleofector II Device, all according to manufacturers’ recommendations.
Ingenio Provides Comparable Efficiency on Amaxa’s Nucleofector Device
Prove it to Yourself −Try a FREE Sample of Ingenio Solution Today!
High Efficiency Electroporationx Save money and reduce research costs while maximizing results
x Compatible with conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®
x Ideal for plasmid or siRNA electroporation
x High efficiency electroporation of hard to transfect cell lines and primary cells
0
20
40
60
80
100
% E
GFP
Pos
itiv
e C
ells
HL-60 K-562 Jurkat E6-1 SK-N-MC
Ingenio® Solution using Gene Pulser
Ingenio® Solution using Amaxa® Nucleofector®
Amaxa® Nucleofector® Solution V on Amaxa® Nucleofector®
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FOR TECHNICAL SUPPORT | Tel +1.608.441.2852 | www.TheTransfectionExperts.com
www.TheTransfectionExperts.com
Ingenio® Electroporation Kits (solution, 0.2 cm cuvettes, cell droppers)
PRODUCT NO. SIZE
MIR 50112 25 RXNa
MIR 50115 50 RXNa
MIR 50118 100 RXNa
Ingenio® Electroporation Kits (solution, 0.4 cm cuvettes, cell droppers)
PRODUCT NO. SIZE
MIR 50113 25 RXNa
MIR 50116 50 RXNa
MIR 50119 100 RXNa
*Primary cell types
Figure 2. Ingenio® Electroporation Kits were used to transfect representative cell types using Amaxa® Nucleofector®. Cells were assayed at 24 hours post-electroporation by flow cytometry and reported as percentage of live cell population. Visit www.TheTransfectionExperts.com for ideal pulse conditions.
©2011 All rights reserved. Mirus Bio LLC. All trademarks are the property of their respective owners.FLING-09/2011
Efficient Plasmid DNA Delivery into Any Cell Type Including Primary Cells
HL-
60
Jurk
at E
6-1
K562
THP-
1
*Ker
atin
ocyt
e
CHO
-K1
HU
V-EC
HeL
a
BH
K-2
1
SK-N
-MC
Hep
G2
HEK
-293
COS-
7
Vero
NIH
-3T3
MCF
-7
Hep
a-SE
AP
NIK
SPC
-12
SK-B
R-3
0
20
40
60
80
100%
EGF
P Po
sitiv
e Ce
lls
*Rat
Cor
tical
Neu
ron
*MEF
Ingenio® Electroporation SolutionPRODUCT NO. SIZE
MIR 50111 25 RXNa
MIR 50114 50 RXNa
MIR 50117 100 RXNa
Cuvettes and cell droppers also sold separately.
a Number of electroporations per kit.
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