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Edwin C. Atabay and Eufrocina P. AtabayReproductive Biotechnology and Physiology Unit
Philippine Carabao Center, Science City Of Munoz, Nueva Ecija
Presented during the 19th Dairy Congress and ExpoDumaguete City, April 13-15, 2016
Intensified Reproductive Management
Program for Dairy
Theoretical Framework:
GAPS/Concerns
• Low AI efficiency /conception rate
• Poor estrus manifestation and detection
• Breeding Seasonality
• High incidence of acyclic/ anestrus and anovularanimals
• Estrus Synch and AI techniques
• Long Calving interval
• Low Reproductive Efficiency
• Costly dairy production
INTERVENTIONS
• Good Nutrition
• Good Animal Health Care
• Effective Breeding/
Reproductive Management Program
EXPECTED OUTCOMES
• ↑ AI efficiency
• ↑Animal Productivity
• ↑ Milk Production
• ↑income
• Vibrant Dairy Industry
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Intensified Reproductive Management
Program for Water Buffaloes: The Triple E Strategy: Formula
• Enhancing Pregnancy RateEPR
• Early Pregnancy DetectionEPD
• Effective Rebreeding Program
ERP
Animal
Productivity,
Reproductive
EfficiencyEPR EPD ERP
Enhancing
Pregnancy
Rate
Early
Pregnancy
Diagnosis
Effective
Rebreeding
Program
Ovarian
Physiology
Intensified Reproductive Management
Program for Water Buffaloes: The Triple E Features
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Intensified Reproductive Management
Program for Water Buffaloes:
The Triple E Strategic Framework
What is Fixed Time AI or Timed AI?
• Fixed Time AI is synchronization of ovulation not estrus among animals
• Time of AI is fixed after Ovulation Synchronization
• There is no need for estrus detection,
• Tight synchrony of ovulation,
• PGF2-based + GnRH
• OVYSYNCH PROTOCOL is the original ovulation synchronization developed to improve reproductive efficiency in cattle (Pursely et al, 1995),
• Since then, various protocols evolved and up until now researches are actively pursued to further increase FTAI efficiency.
• The reason for the tremendous progress of the dairy industry in the US
• Revolutionized the meat industry in Brazil
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Conventional AI vs Fixed Time AI
Conventional AI FTAI
• Synchronization of
estrus
Synchronization of
ovulation
• Needs close heat
observation
Does not need estrus
detection
• Uses PGF2 alpha PGF2 + GnRH
• Ovulation duration :
variable
short
• Lower pregnancy Higher pregnancy
Applications of FTAI in Buffaloes
• Use in repeat breeders/dairy animals
• Managing difficult breeding in heifers
• Creating a contemporary herd, in PC/native buffaloes
for managing breeding and performance data.
• Possibility of facilitating year-round calf and milk
production
• Enhance related reproductive technologies such as
MOET where FTAI is part of the program.
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FTAI Protocol for Cycling Animals: FTAI Protocol for Cycling Animals:
OVSYNCH PROTOCOLOVSYNCH PROTOCOL
PROCEDURE :
DAY 0: Inject GnRH to ovulate dominant follicle and start a new
follicular wave (ie: 3-5pm)
DAY7: Inject PGF2a to regress CL (ie:3-5 pm)
DAY9: Inject GnRH to ovulate follicle (ie: 3-5 pm)
DAY 10: Timed AI 14-16h after the second GnRH injection
FTAI Protocol for Non-cycling Animals:
P4-OVSYNCH
PROCEDURE:PROCEDURE:DAYDAY--0: Insert 0: Insert P4 and P4 and inject GnRH inject GnRH
DAYDAY--7: Remove 7: Remove P4 and P4 and Inject with Inject with PGF2aPGF2aDAYDAY--9: Inject 9: Inject 22ndnd GnRH GnRH to ovulate follicle to ovulate follicle DAY DAY 10: 10: Timed Timed AI (am and pm) 14AI (am and pm) 14--16 16 hrhr after the after the second GnRH injectionsecond GnRH injection
Day 0 Day 9Day 7
GnRH
+
P4
PGF2 alpha
Removal P4
Progesterone
GnRH
Day 10/11
Ovulation
1614-16h
AI
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Major FTAI Activities
Follicular Development and
Endocrine Profile of Ovulation and FTAI Protocols
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0
10
20
30
40
50
0
5
10
15
20
4/1 4/8 4/15 4/22 4/29 5/6 5/13
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PGF2α
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4/4
PGF2α
4/7 4/9 4/15
PGF2α
4/17 4/19 4/24
E2
4/28
FSH 7AU
4/30
FSH 5AU
PGF2α
L
R
Cow No.1
�FSH
P4
E2
Pro
ge
ste
ron
e (
ng
/ml) E
strad
iol (p
g/m
l)
Date No.of L R
4/30 FL 7 8
5/9 CL 8 8
embryo ? ?
CIDR
Characteristics/Parameters of Effective
FTAI Protocol
Must be able to:
• induce estrus (estradiol prod’n)
• achieve the expected ovulatory follicle size
• successfully induce ovulation
• form functional Corpus luteum (CL)
high conception rate
high calf drop
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Transrectal Pregnancy Diagnosis
• Early pregnancy detection
• Embryonic mortality
• Fetal resorption
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DOST-PCAARRD-FUNDED MILK PRODUCTION
PROGRAM
Enhancing Milk Production of Water Buffaloes Enhancing Milk Production of Water Buffaloes
through through S&T S&T InterventionsInterventionsDOSTDOST--PCAARRD/DAPCAARRD/DA--PCC PROGRAMPCC PROGRAM
Program Leader: Dr. Program Leader: Dr. ArnelArnel N. Del BarrioN. Del Barrio
Project Components:
1. Development of Feeding Protocols and Practices to Support the Nutritional
Requirements of Dairy Buffaloes: Dr. Daniel Aquino
2. Development of Reproductive Management Program for Increased
Efficiency of AI in Dairy Buffaloes: Dr. Edwin Atabay
3. Development of Health Care Technologies and Practical Farm Practices in
Support of Increasing Buffalo Milk Production: Dr. Michelle Balbin
4. Milk Quality and Safety Assurance from Farm to Milk Processing Plant:
Ms Mina Abella
5. Strengthening the San Agustin Crossbred Carabao-Based Enterprise
Development (CBED) Model: Dr. Annabelle Sarabia
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The Triple E Strategy:
Research and Development Direction
• Development of Strategic Reproductive Mgt. Program for
livestock:
◊ climate change-responsive, (breeding seasonality)
◊ age group/classification-specific (heifer, post partum)
◊ ovarian structure/condition-specific (with or w/o CL)
◊ production system-specific ( individual, group)
• Expanded utilization of Reproductive Biotechnologies :
Ovum-Pick UP
In Vitro Embryo Production
Multiple Ovulation and Embryo Transfer
Oocyte and Embryo Cryopreservation
DAIRY
BOOM
Join the Dairy Program Revolution!!!
GATAS PINOY:PAUNLARIN,
TANGKILIKIN, PALAGANAPIN!!!
……..towards a sustainable and globally competitive dairy industry
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LIVESTOCK BIOTECHNOLOGY CENTERPhilippine Carabao Center, Science City of Munoz, Nueva Ecija
Contact address:
Eufrocina P. Atabay
Email add: [email protected]
Mobile No.: 09178133389
Contact address:
Edwin C. Atabay
Email add: [email protected]
Mobile No.: 09175014222