Harvesting of a fragile alga Dunaliella salina by membrane filtration with permeate recovery for microalgae cultivation
Joana Montea, Marta Sáa, Celina Parreirab, Rui Motab, Joana Galanteb, Pedro Nascimentob, Filipe Semiãob, Cláudia F. Galinhaa, Vanessa J. Pereiraa, Diana Fonsecab, Luís Costab, Vítor Verdelhob, Fons Jacobsc, Carla Brazinhaa, João G. Crespoa
Introduction
TitleText
Permeate Recycling
Harvesting and Monitoring
ThisprojecthasbeenfundedbytheEuropeanCommissionunderthe7thFrameworkProgrammeforResearchandDevelopmentCollaboraAveproject–GAno613870
www.d-factoryalgae.eu
D-Factory Partners P Harvey, P Lucas, V Verdelho, L Costa, D Fonseca, D Rooke, C Bright, F Jacobs, P Goacher, J Crespo, C Brazinha, G Reinhardt, H Keller, L Martinelli, D Schroeder, N Igl, A Kokossis, M Psycha, A Ben-Amotz, K Persson, L Brive, I Mira, D. Pen ̃alosa, C Casanovas, D Arroyo
Contact Details Carla [email protected] , Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal
aiBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal bA4F – Algae for future, Campus do Lumiar, Estrada do Paço do Lumiar, Edif. E, R/C, 1649-038 Lisboa,Portugal
cEvodos, Weegbree 21, 4941 VT, Raamsdonksveer, the Netherlands
Low-shearcentrifuge(SpiralPlateTechnology,SPT,Evodos)Membraneunits
Pre-concentra*onofbiomassbymembraneprocessingpriortocentrifuga*on- Reduceoverallenergyandcapitalcosts- Maximisevolumetricfluxeswhilemaintainingcellintegrityatmaximisedvaluesof
concentraDonfactors
12.5%(5.43h)
0
10
20
1 5 9 13 17
%Lossofintegrity
CFoverall(-)
1stHarves6ng
2ndHarves6ng
3rdHarves6ng
“Green”D.salina “Orange”D.salina
0
15
30
1 9 17 25 33
%Lossofintegrity
CFoverall(-)
1stHarves0ng
2ndHarves0ng
3rdHarves0ng
0
20
40
1 5 9 13 17
Jv (L
/(m2 .
h))
1stHarves1ng
2ndHarves1ng
3rdHarves1ng
CFoverall=16.4(or94%permeaterecovery)Jvaverage=22L/(m2.h)
Cellintegrityloss=13%
CFoverall=36.7(or97%permeaterecovery)Jv=10L/(m2.h)
Cellintegrityloss=7%
2DFluorescenceSpectroscopy
λ em (nm)
250 300 350 400 450 500 550 600 650
λ ex
(nm
)
300
350
400
450
500
550
600
650
700
I II
III
I. ProteinsII. HumiccompoundsIII. Pigments
0,0E+0
4,0E+5
8,0E+5
1,2E+6
0,0E+0 4,0E+5 8,0E+5 1,2E+6
CellCo
nc.(cells/m
L)
Observed
CellConc.(cells/mL)Predicted
2σ Train.:R2=0.86,Slope=1.00
Valid.:R2=0.83,Slope=0.78
RMSEP=1.3E+5cells/mL
Train.:R2=0.91,Slope=1.00
Valid.:R2=0.82,Slope=0.70
RMSEP=5.3%viability
60
70
80
90
100
110
60 70 80 90 100 110
Viability(%
)Observed
Viability(%)Predicted
0,0E+0
1,0E+7
2,0E+7
0,0E+0 1,0E+7 2,0E+7
CellsCon
c.(cells/mL)
Observed
CellConc.(cells/mL)Predicted
Train.:R2=0.93,Slope=1.00
Valid.:R2=0.93,Slope=1.15
RMSEP=1.4E+6cells/mL
Train.:R2=0.76,Slope=1.00
Valid.:R2=0.69,Slope=1.10
RMSEP=10%viability
20
40
60
80
100
20 70
Viability(%
)Observed
Viability(%)Predicted
Cellnumber
PrincipalComponentAnalysis(PCA)
ProjecDontoLatentStructures(PLS)
findcorrelaDonstopredictcellulargrowthandotherkeyperformanceparameters
§ Cellnumber§ Cellintegrity§ Glycerol§ Pigments§ Enzymes§ Proteins
300 400 500 600 700
300
400
500
600
700
X Axis Title
Y Ax
is Ti
tle
0
25.00
50.00
75.00
100.0
125.0
150.0
175.0
200.0
Train.:R2=0.93,Slope=1.00
Valid.:R2=0.92,Slope=1.11
RMSEP=0.23absunits(at410nm)
0
1
2
3
4
0 1 2 3 4
UnitsofA
bs410nm
Observed
UnitsofAbs410nmPredicted
%Viability
ExtracellularGlycerol
91mg/L
262mg/L
137mg/L
0
100
200
300
0 1 2 3 4 5 6
Glycerolco
nten
t(mg/L)
Time(h)
UV+H2O2
Ozono+H2O2
0
25
50
0 1 2 3 4 5 6
Nitrites&
Nitra
tes(mg/L)
Time(h)
Ozono+H2O2
UV+H2O2
Permeaterecoveryformicroalgaecul*va*onmedia
Mediumrecycling
Maximumvolumetricproduc*vityofcarotenoids(mgCar/L/d)
Permeatewithouttreatment 6.93±0.48
Permeate+UV+H2O2 5.98±0.41
Evodos Dynamic settler
liters/hour Intactcellsharvested dryweightcontentEvodos10 250 Yes,>95% 20-30%Evodos10 300 Yes,>95% 20-30%
Max.capacity350liters/hour
Evodos50 harvest
• Dunaliellaintactalgae• 40%solids• highbeta-carotenetoabove10%
AFDW
Evodos50:-4000L/h-95%separaDonefficiency->20h/day
Conclusions
Evodos10 harvest
EconomicEvalua*onofHarves*ng“green”Dunaliellasalina
ü ReducDonofOPEX+CAPEXof52%
ü ReducDonofenergy
consumpDonof45%
ü Harves*ngof“green”DunaliellasalinabyultrafiltraDonallowedafinalCFof16.4andJv.averageof22L/(m2.h)withalossofintegrityof13%
ü Harves*ngof“orange”DunaliellasalinabyultrafiltraDonallowedanoverallCFof36.7andJvof10L/(m2.h)withalossofintegrityof7%
ü Reduc*onoftheOPEX+CAPEXof52%andreduc*onofenergyconsump*onof45%
ü FluorescencespectradisDnguishdifferencesinmicroalgaemedia.ModelforDunaliellasalinaculturespredictedcellconcentraDonandintegrity
ü UltrafiltraDonpermeatetreatedbyUV+H2O2allowsformediumrecyclingforDunaliellasalinaculture
ü SuccessfulharvesDngofDunaliellasalinawithEvodosSpiralPlateTechnology;intact/undamagedalgae,highsolidcontent,>95%separa*onefficiency
ü SuccessfuldevelopmentfromPilotEvodos10unittoindustrialautomatedEvodos50unit
Basedon:EnergyconsumpDon(membraneunit)=0.87kWh/m3
EnergyconsumpDon(centrifuge)=2.5kWh/m3
References[1]MonteJ.,etal,Sep.Pur.Technol.,2018,190,252,260[2]SáM.,elal,AlgalResearch,2017,24,325–332
0
10
20
1 9 17 25 33
Jv(L/(m
2 .h))
1stHarves0ng2ndHarves0ng3rdHarves0ng
