Thermo Scientific
protein ladders give you
choices for protein molecular weight estimation.
Thermo Scientific™ Pierce™ Prestained and Unstained Protein Ladders for easy, reliable protein molecular weight estimation.
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
Thermo Scientific SuperSignal Molecular Weight Protein LaddersThe Thermo Scientific™ SuperSignal™ Molecular Weight Protein Ladders contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 20 to 150kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. Use the SuperSignal Enhanced Molecular Weight Protein Ladders with mouse monoclonals.
150kDa
100kDa
80kDa
60kDa
50kDa
40kDa
30kDa
20kDa
4-12% Bis-Tris 4-20% Tris-glycine
5µL 10µL 5µL 10µL
Thermo Scientific Pierce Protein Molecular Weight MarkerThermo Scientific™ Pierce™ Prestained Protein Molecular Weight Markers are designed to monitor protein migration during SDS-polyacrylamide gel electrophoreses and monitor protein transfer onto membranes. Pierce Unstained Protein Weight Markers produce sharp bands on SDS-polyacrylamide gel following staining with Coomassie-based or silver stains and are recommended for accurate sizing of proteins on SDS PAGE and Western blots.
Thermo Scientific PageRuler Unstained Protein LaddersThermo Scientific™ PageRuler™ Unstained Protein Ladders are mixtures of recombinant, highly purified proteins designed for accurate sizing of proteins by SDS-PAGE. The ladders resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with Coomassie Blue or silver stain. Each ladder contains protein bands of greater intensity which serve as reference bands. Proteins can be detected on Western blots by staining with Ponceau S, Coomassie Blue, (except for 3.4kDa and 5kDa polypeptides) or by using Strep-Tactin™ conjugates.
Thermo Scientific PageRuler Prestained Protein LaddersThe Thermo Scientific™ PageRuler™ and PageRuler Plus Prestained Protein Ladders are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein molecular weight determination. The green and orange reference bands in the PageRuler products provide easy orientation of the protein bands.
Thermo Scientific Spectra Multicolor Protein LaddersFour different chromophores bound to proteins in Thermo Scientific™ Spectra™ Multicolor Broad Range Protein Ladders produce a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range and Low Range Protein Ladders are designed for large and small protein analysis, respectively.
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26623, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26623, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26623, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26623, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
Prestained or unstained, including specialty protein ladders. Choose what’s best for your application.
Thermo Scientific PageRuler Prestained NIR LadderThe Thermo Scientific™ PageRuler™ Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250kDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing. The protein MW markers in this ladder resolve into sharp bands when analyzed by SDS-PAGE and are labeled with a fluorescent dye for visualization with instruments equipped with near-infrared (NIR) fluorescence detection. The bands are also directly visible because the proteins are prestained blue.
PageRuler NIR Protein Ladder
4-20% Tris-glycine SDS-PAGEProduct # 26635
250
130
95
70
55
43
3426
15
11
–
–
–
–
––
––
–
–
kDa
Visi
ble
Ligh
t
Nea
r-IR
Imag
er
–
–
–
–
––
––
–
–
Our ready-to-use mixes of recombinant proteins facilitate easy protein analysis during gel electrophoresis and Western blotting. The Prestained Protein Ladders are recommended for approximate determination of molecular weight, monitoring the progress of the electrophoresis run and the efficiency of the protein transfer to the membrane during Western blotting procedures. For precise determination of molecular weights of target proteins in any buffer system, we recommend using our Unstained Protein Ladders.
Highlights:• Best value – high quality without the high price
• Performance – sharp protein band resolution and consistent migration patterns provide easy molecular weight determination
• Convenient – protein ladders are ready to load with no heating or boiling required
• Reliable – exceptional lot-to-lot consistency and reproducibility
Thermo Scientific
protein ladders give you
choices for protein molecular weight estimation.
Thermo Scientific™ Pierce™ Prestained and Unstained Protein Ladders for easy, reliable protein molecular weight estimation.
Product #’s PI84785, PI84786 Product # PI26635
Product # PI26610Product # PI26612Product # PI26614 Product # PI26630 Product # PI26632
Product #’s PI26616, PI26617 Product #’s PI26619, PI26620 Product #’s PI26623, PI26634 Product # PI26625Product # PI26628
BN03
3014
1 0
4/14
Prin
ted
in th
e U.
S.
thermoscientific.com/pierce© 2014 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only. Ready Gel is a registered trademark of Bio-Rad Laboratories, Inc. Strep-Tactin is a registered trademark of IBA GmbH. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
In the United States:For customer service, call 1-800-766-7000To fax an order, use 1-800-926-1166To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437To fax an order, use 1-800-463-2996To order online: www.fishersci.ca
© 2013 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
Reach new heights in gel electrophoresis
0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300
Spectra Multicolor High Range
Spectra Multicolor Broad Range
PageRuler Unstained Broad Range
PageRuler Plus Prestained Ladder
PageRuler Unstained Ladder
PageRuler Prestained Ladder
Pierce Prestained Marker
Pierce Unstained Marker
PageRuler Unstained Low Range
Spectra Multicolor Low Range
Protein Ladder Range (kDa)
The bars indicate the ranges of each product. Diamonds indicate the location and color of protein bands in each product.
Unstained Ladder
Prestained Ladder
Selection Table for Thermo Scientific Pierce MW Markers and Protein Ladders
Reference Guide: Migration Patterns in Different Electrophoresis Conditions
Mig
ratio
n Pa
ttern
s of
Spe
ctra
Mul
ticol
or
High
Ran
ge a
nd L
ow R
ange
Pro
tein
Lad
der
Mig
ratio
n Pa
ttern
s of
Spe
ctra
Mul
ticol
or
Broa
d Ra
nge
Prot
ein
Ladd
erM
igra
tion
Patte
rns
of P
ageR
uler
Pr
esta
ined
Pro
tein
Lad
der
Mig
ratio
n Pa
ttern
s of
Pag
eRul
er P
lus
Pres
tain
ed P
rote
in L
adde
r
SP – small peptide SDS-polyacrylamide gel with higher degree of crosslinking (c=5% instead of usual ~3%).‡ ‡ – migration patterns were determined using respective Novex™ precast gels.††† – migration patterns were determined using respective Ready Gel™ precast gel.
% le
ngth
of g
el 40
25
15
1052
40
25
15
10
52
40
25
15
10
5
2
40
25
15
10
5
40
25
15
10
5
2
40
25
15
10
5
40
25
15
10
52
40
25
15
10
52
40
25
15
1052
40
25
15
105
40
15
1052
Thermo Scientific Spectra Multicolor Low Range Protein LadderGel type Tris-Tricine
Gel concentration 4-20% 10-20% 14% 16% 18% 14-SP % 16-SP % 18-SP % 10-20%‡ ‡ 16%‡ ‡ 10-20%† † †
Running buffer Tris-Tricine SDS
Apparent Molecular Weights (kDa)
10
20
30
40
50
60
70
80
90
100
% le
ngth
of g
el
300250
180
130
100
70
5040
300
250
180
130
100
300250180
130
100
70
50
40
300
250
180
130
100
70
5040
270205
150
120
85
65
50
40
270205150120
85
65
50
40
270185140115
80
65
50
40
300250180
130
100
70
50
40
300250180130100
70
50
40
Thermo Scientific Spectra Multicolor High Range Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-12% 4% 6% 8% 10% 4-20% 3-8% 7% 4-12%Running buffer Tris-Glycine Tris-Acetate MOPS
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
% le
ngth
of g
el
260
140100
70
504035
25
15
10
260
14010070
50
40
35
25
15
10
260
140
100
70
50
40
35
25
15
260
140100
70
50
40
35
25
15
26014010070
50
4035
25
15
10
26014010070
50
40
35
25
15
10
225
120
85
65
40
50
3025
225
120
85
65
40
50
30
25
225
115
80
65
35
50
30
25
1510
225
11580
65
35
50
30
25
15
225
1158065
35
50
30
25
15
235
120
8070
40
50
30
25
15
10
235
1208070
40
50
30
25
15
10
235
1208070
40
50
30
25
15
10
260
140
100
70
50
4035
25
15
10
Thermo Scientific Spectra Multicolor Broad Range Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
205
120
85
65
30
50
25
250
100
70
35
55
25
15
130
190
11580
70
30
50
25
15
10
190
1158070
30
50
25
15
10
185
115
80
65
30
50
25
1510
250
100
70
35
55
25
15
10
130
250
10070
35
55
25
15
10
130
250
10070
35
55
25
15
10
130
250
100
70
35
55
25
15
130
250
10070
35
55
25
130
10
250
10070
35
55
25
15
130
10
205
120
85
65
30
50
25
185
1158065
30
50
25
15
185
1158065
30
50
25
15
190
1158070
30
50
25
15
10
% le
ngth
of g
el
15
Thermo Scientific PageRuler Plus Prestained Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
17013010070
55
40
35
25
15
10
170
130
100
70
55
40
35
25
15
10
17013010070
55
4035
25
15
10
170130
100
70
55
40
35
25
15
170130100
70
55
40
35
25
15
10
17013010070
55
40
35
25
15
10
17013010070
55
40
35
25
15
10
150
120
85
65
50
40
3025
150
120
85
65
50
40
30
25
115140
80
65
50
40
30
25
15
10
1401158065
50
40
30
25
15
1401158065
50
40
30
25
15
1401158065
50
40
30
25
15
10
1401158070
50
40
30
25
15
10
1401158070
5040
30
25
15
10
Note: The apparent molecular size of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.
†† – Migration patterns were determined using respective NuPAGE™ precast gels.
% le
ngth
of g
el
Thermo Scientific PageRuler Prestained Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
Choosing the right protein ladder. Use this table as a quick reference guide to select the right protein ladder for your application.
Application
Thermo Scientific Pierce Protein Ladders SDS PAGE Western Blotting
Product Name Product # # ProteinsMW range
Reference bands
PageRuler Unstained Broad Range Protein Ladder
PI26630 11 5-250kDa 20, 50 and 100kDa
Best — — Best — — Good†
PageRuler Unstained Protein Ladder
PI26614 14 10-200kDa 50kDa Good — — Good — — Good†
PageRuler Unstained Low Range Protein Ladder
PI26632 8 3.4-100kDa 25kDa Best — — Best — — Good†
PageRuler Unstained High Range Protein Ladder
PI26637 8 60-250kDa 150kDa Best — — Best — — Good†
Pierce Unstained Protein Molecular Weight Marker
PI26610 7 14.4-116kDa
— Good — — Good — — —
PageRuler Prestained Protein Ladder
PI26616, PI26617
10 10-170kDa Green 10kDa; Orange 70kDa
Good Good Good — Good‡ Good —
PageRuler Plus Prestained Protein Ladder
PI26619, PI26620
9 10-250kDa Green 10kDa; Orange 25 and 70kDa
Good Good Good — Good‡ Good —
Spectra Multicolor Broad Range Protein Ladder
PI26634, PI26623
10 10-260kDa Green 10 and 50kDa; Orange 40, 70 and 260kDa; Pink 140kDa
Good Best Best — Good‡ Best —
Spectra Multicolor Low Range Protein Ladder
PI26628 6 1.7-40kDa Green 10kDa; Orange 40kDa
Good Best Best — Good‡ Best —
Spectra Multicolor High Range Protein Ladder
PI26625 8 40-300kDa Green 50kDa; Orange 70 and 300kDa
Good Best Best — Good‡ Best —
Pierce Prestained Protein Molecular Weight Marker
PI26612 6 20-120kDa — Good Good Good — Good Good —
SuperSignal Molecular Weight Protein Ladder
PI84785, PI84786
8 20-150kDa — Good — — Good — Good Best
PageRuler Prestained NIR Protein Ladder
PI26635 10 11-250kDa 55kDa Good Good Good — Best Good —
† with Strep-Tactin™ HRP or AP Conjugates‡ Blue and green prestained bands fluoresce in NIR region
Unst
aine
d
Chemi. Lum.
NIR
Colo
rimet
ric
Pres
tain
ed
Prot
ein M
W
dete
rmin
ation
Prot
ein
band
visu
aliza
tion
Mon
itorin
g
elect
roph
ores
is
run Co
omas
sie/s
ilver
/
fluor
esce
nt
stain
ing
NIR
de
tect
ion
Mon
itorin
g pr
otein
trans
fer
Chem
ilum
ines
cent
band
visu
aliza
tion
thermoscientific.com/pierce
thermoscientific.com/pierce
Ordering Information
Product # Description Pkg. Size
PI26610 Pierce Unstained Protein MW Marker 2 x 1mL
PI26612 Pierce Prestained Protein MW Marker 500µL
PI26614 PageRuler Unstained Protein Ladder 2 x 250µL
PI26616 PageRuler Prestained Protein Ladder 2 x 250µL
PI26617 PageRuler Prestained Protein Ladder 10 x 250µL
PI26619 PageRuler Plus Prestained Protein Ladder 2 x 250µL
PI26620 PageRuler Plus Prestained Protein Ladder 10 x 250µL
PI26634 Spectra Multicolor Broad Range Protein Ladder 2 x 250µL
PI26623 Spectra Multicolor Broad Range Protein Ladder 10 x 250µL
PI26625 Spectra Multicolor High Range Protein Ladder 2 x 250µL
PI26628 Spectra Multicolor Low Range Protein Ladder 250µL
PI26630 PageRuler Unstained Broad Range Protein Ladder 2 x 250µL
PI26632 PageRuler Unstained Low Range Protein Ladder 2 x 250µL
PI84785 SuperSignal Molecular Weight Protein Ladder 250µL
PI84786 SuperSignal Enhanced Molecular Weight Protein Ladder
250µL
PI26635 PageRuler Prestained NIR Protein Ladder 2 x 250µL
Reach new heights in gel electrophoresis
0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300
Spectra Multicolor High Range
Spectra Multicolor Broad Range
PageRuler Unstained Broad Range
PageRuler Plus Prestained Ladder
PageRuler Unstained Ladder
PageRuler Prestained Ladder
Pierce Prestained Marker
Pierce Unstained Marker
PageRuler Unstained Low Range
Spectra Multicolor Low Range
Protein Ladder Range (kDa)
The bars indicate the ranges of each product. Diamonds indicate the location and color of protein bands in each product.
Unstained Ladder
Prestained Ladder
Selection Table for Thermo Scientific Pierce MW Markers and Protein Ladders
Reference Guide: Migration Patterns in Different Electrophoresis ConditionsM
igra
tion
Patte
rns
of S
pect
ra M
ultic
olor
Hi
gh R
ange
and
Low
Ran
ge P
rote
in L
adde
r
Mig
ratio
n Pa
ttern
s of
Spe
ctra
Mul
ticol
or
Broa
d Ra
nge
Prot
ein
Ladd
erM
igra
tion
Patte
rns
of P
ageR
uler
Pr
esta
ined
Pro
tein
Lad
der
Mig
ratio
n Pa
ttern
s of
Pag
eRul
er P
lus
Pres
tain
ed P
rote
in L
adde
r
SP – small peptide SDS-polyacrylamide gel with higher degree of crosslinking (c=5% instead of usual ~3%).‡ ‡ – migration patterns were determined using respective Novex™ precast gels.††† – migration patterns were determined using respective Ready Gel™ precast gel.
% le
ngth
of g
el 40
25
15
1052
40
25
15
10
52
40
25
15
10
5
2
40
25
15
10
5
40
25
15
10
5
2
40
25
15
10
5
40
25
15
10
52
40
25
15
10
52
40
25
15
1052
40
25
15
105
40
15
1052
Thermo Scientific Spectra Multicolor Low Range Protein LadderGel type Tris-Tricine
Gel concentration 4-20% 10-20% 14% 16% 18% 14-SP % 16-SP % 18-SP % 10-20%‡ ‡ 16%‡ ‡ 10-20%† † †
Running buffer Tris-Tricine SDS
Apparent Molecular Weights (kDa)
10
20
30
40
50
60
70
80
90
100
% le
ngth
of g
el
300250
180
130
100
70
5040
300
250
180
130
100
300250180
130
100
70
50
40
300
250
180
130
100
70
5040
270205
150
120
85
65
50
40
270205150120
85
65
50
40
270185140115
80
65
50
40
300250180
130
100
70
50
40
300250180130100
70
50
40
Thermo Scientific Spectra Multicolor High Range Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-12% 4% 6% 8% 10% 4-20% 3-8% 7% 4-12%Running buffer Tris-Glycine Tris-Acetate MOPS
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
% le
ngth
of g
el
260
140100
70
504035
25
15
10
260
14010070
50
40
35
25
15
10
260
140
100
70
50
40
35
25
15
260
140100
70
50
40
35
25
15
26014010070
50
4035
25
15
10
26014010070
50
40
35
25
15
10
225
120
85
65
40
50
3025
225
120
85
65
40
50
30
25
225
115
80
65
35
50
30
25
1510
225
11580
65
35
50
30
25
15
225
1158065
35
50
30
25
15
235
120
8070
40
50
30
25
15
10
235
1208070
40
50
30
25
15
10
235
1208070
40
50
30
25
15
10
260
140
100
70
50
4035
25
15
10
Thermo Scientific Spectra Multicolor Broad Range Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
205
120
85
65
30
50
25
250
100
70
35
55
25
15
130
190
11580
70
30
50
25
15
10
190
1158070
30
50
25
15
10
185
115
80
65
30
50
25
1510
250
100
70
35
55
25
15
10
130
250
10070
35
55
25
15
10
130
250
10070
35
55
25
15
10
130
250
100
70
35
55
25
15
130
250
10070
35
55
25
130
10
250
10070
35
55
25
15
130
10
205
120
85
65
30
50
25
185
1158065
30
50
25
15
185
1158065
30
50
25
15
190
1158070
30
50
25
15
10
% le
ngth
of g
el
15
Thermo Scientific PageRuler Plus Prestained Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
17013010070
55
40
35
25
15
10
170
130
100
70
55
40
35
25
15
10
17013010070
55
4035
25
15
10
170130
100
70
55
40
35
25
15
170130100
70
55
40
35
25
15
10
17013010070
55
40
35
25
15
10
17013010070
55
40
35
25
15
10
150
120
85
65
50
40
3025
150
120
85
65
50
40
30
25
115140
80
65
50
40
30
25
15
10
1401158065
50
40
30
25
15
1401158065
50
40
30
25
15
1401158065
50
40
30
25
15
10
1401158070
50
40
30
25
15
10
1401158070
5040
30
25
15
10
Note: The apparent molecular size of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.
†† – Migration patterns were determined using respective NuPAGE™ precast gels.
% le
ngth
of g
el
Thermo Scientific PageRuler Prestained Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
Choosing the right protein ladder. Use this table as a quick reference guide to select the right protein ladder for your application.
Application
Thermo Scientific Pierce Protein Ladders SDS PAGE Western Blotting
Product Name Product # # ProteinsMW range
Reference bands
PageRuler Unstained Broad Range Protein Ladder
PI26630 11 5-250kDa 20, 50 and 100kDa
Best — — Best — — Good†
PageRuler Unstained Protein Ladder
PI26614 14 10-200kDa 50kDa Good — — Good — — Good†
PageRuler Unstained Low Range Protein Ladder
PI26632 8 3.4-100kDa 25kDa Best — — Best — — Good†
PageRuler Unstained High Range Protein Ladder
PI26637 8 60-250kDa 150kDa Best — — Best — — Good†
Pierce Unstained Protein Molecular Weight Marker
PI26610 7 14.4-116kDa
— Good — — Good — — —
PageRuler Prestained Protein Ladder
PI26616, PI26617
10 10-170kDa Green 10kDa; Orange 70kDa
Good Good Good — Good‡ Good —
PageRuler Plus Prestained Protein Ladder
PI26619, PI26620
9 10-250kDa Green 10kDa; Orange 25 and 70kDa
Good Good Good — Good‡ Good —
Spectra Multicolor Broad Range Protein Ladder
PI26634, PI26623
10 10-260kDa Green 10 and 50kDa; Orange 40, 70 and 260kDa; Pink 140kDa
Good Best Best — Good‡ Best —
Spectra Multicolor Low Range Protein Ladder
PI26628 6 1.7-40kDa Green 10kDa; Orange 40kDa
Good Best Best — Good‡ Best —
Spectra Multicolor High Range Protein Ladder
PI26625 8 40-300kDa Green 50kDa; Orange 70 and 300kDa
Good Best Best — Good‡ Best —
Pierce Prestained Protein Molecular Weight Marker
PI26612 6 20-120kDa — Good Good Good — Good Good —
SuperSignal Molecular Weight Protein Ladder
PI84785, PI84786
8 20-150kDa — Good — — Good — Good Best
PageRuler Prestained NIR Protein Ladder
PI26635 10 11-250kDa 55kDa Good Good Good — Best Good —
† with Strep-Tactin™ HRP or AP Conjugates‡ Blue and green prestained bands fluoresce in NIR region
Unst
aine
d
Chemi. Lum.
NIR
Colo
rimet
ric
Pres
tain
ed
Prot
ein M
W
dete
rmin
ation
Prot
ein
band
visu
aliza
tion
Mon
itorin
g
elect
roph
ores
is
run Co
omas
sie/s
ilver
/
fluor
esce
nt
stain
ing
NIR
de
tect
ion
Mon
itorin
g pr
otein
trans
fer
Chem
ilum
ines
cent
band
visu
aliza
tion
thermoscientific.com/pierce
thermoscientific.com/pierce
Ordering Information
Product # Description Pkg. SizeU.S. Price
PI26610 Pierce Unstained Protein MW Marker 2 x 1mL $ 58
PI26612 Pierce Prestained Protein MW Marker 500µL $ 58
PI26614 PageRuler Unstained Protein Ladder 2 x 250µL $ 82
PI26616 PageRuler Prestained Protein Ladder 2 x 250µL $100
PI26617 PageRuler Prestained Protein Ladder 10 x 250µL $454
PI26619 PageRuler Plus Prestained Protein Ladder 2 x 250µL $122
PI26620 PageRuler Plus Prestained Protein Ladder 10 x 250µL $550
PI26634 Spectra Multicolor Broad Range Protein Ladder 2 x 250µL $106
PI26623 Spectra Multicolor Broad Range Protein Ladder 10 x 250µL $477
PI26625 Spectra Multicolor High Range Protein Ladder 2 x 250µL $138
PI26628 Spectra Multicolor Low Range Protein Ladder 250µL $120
PI26630 PageRuler Unstained Broad Range Protein Ladder 2 x 250µL $ 90
PI26632 PageRuler Unstained Low Range Protein Ladder 2 x 250µL $ 90
PI84785 SuperSignal Molecular Weight Protein Ladder 250µL $199
PI84786 SuperSignal Enhanced Molecular Weight Protein Ladder
250µL $199
PI26635 PageRuler Prestained NIR Protein Ladder 2 x 250µL $204
Thermo Scientific SuperSignal Molecular Weight Protein LaddersThe Thermo Scientific™ SuperSignal™ Molecular Weight Protein Ladders contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 20 to 150kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. Use the SuperSignal Enhanced Molecular Weight Protein Ladders with mouse monoclonals.
150kDa
100kDa
80kDa
60kDa
50kDa
40kDa
30kDa
20kDa
4-12% Bis-Tris 4-20% Tris-glycine
5µL 10µL 5µL 10µL
Thermo Scientific Pierce Protein Molecular Weight MarkerThermo Scientific™ Pierce™ Prestained Protein Molecular Weight Markers are designed to monitor protein migration during SDS-polyacrylamide gel electrophoreses and monitor protein transfer onto membranes. Pierce Unstained Protein Weight Markers produce sharp bands on SDS-polyacrylamide gel following staining with Coomassie-based or silver stains and are recommended for accurate sizing of proteins on SDS PAGE and Western blots.
Thermo Scientific PageRuler Unstained Protein LaddersThermo Scientific™ PageRuler™ Unstained Protein Ladders are mixtures of recombinant, highly purified proteins designed for accurate sizing of proteins by SDS-PAGE. The ladders resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with Coomassie Blue or silver stain. Each ladder contains protein bands of greater intensity which serve as reference bands. Proteins can be detected on Western blots by staining with Ponceau S, Coomassie Blue, (except for 3.4kDa and 5kDa polypeptides) or by using Strep-Tactin™ conjugates.
Thermo Scientific PageRuler Prestained Protein LaddersThe Thermo Scientific™ PageRuler™ and PageRuler Plus Prestained Protein Ladders are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein molecular weight determination. The green and orange reference bands in the PageRuler products provide easy orientation of the protein bands.
Thermo Scientific Spectra Multicolor Protein LaddersFour different chromophores bound to proteins in Thermo Scientific™ Spectra™ Multicolor Broad Range Protein Ladders produce a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range and Low Range Protein Ladders are designed for large and small protein analysis, respectively.
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26623, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDaG
el
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26623, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
B
lot
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26623, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26623, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
Prestained or unstained, including specialty protein ladders. Choose what’s best for your application.
Thermo Scientific PageRuler Prestained NIR LadderThe Thermo Scientific™ PageRuler™ Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250kDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing. The protein MW markers in this ladder resolve into sharp bands when analyzed by SDS-PAGE and are labeled with a fluorescent dye for visualization with instruments equipped with near-infrared (NIR) fluorescence detection. The bands are also directly visible because the proteins are prestained blue.
PageRuler NIR Protein Ladder
4-20% Tris-glycine SDS-PAGEProduct # 26635
250
130
95
70
55
43
3426
15
11
–
–
–
–
––
––
–
–
kDa
Visi
ble
Ligh
t
Nea
r-IR
Imag
er
–
–
–
–
––
––
–
–
Our ready-to-use mixes of recombinant proteins facilitate easy protein analysis during gel electrophoresis and Western blotting. The Prestained Protein Ladders are recommended for approximate determination of molecular weight, monitoring the progress of the electrophoresis run and the efficiency of the protein transfer to the membrane during Western blotting procedures. For precise determination of molecular weights of target proteins in any buffer system, we recommend using our Unstained Protein Ladders.
Highlights:• Best value – high quality without the high price
• Performance – sharp protein band resolution and consistent migration patterns provide easy molecular weight determination
• Convenient – protein ladders are ready to load with no heating or boiling required
• Reliable – exceptional lot-to-lot consistency and reproducibility
Thermo Scientific
protein ladders give you
choices for protein molecular weight estimation.
Thermo Scientific™ Pierce™ Prestained and Unstained Protein Ladders for easy, reliable protein molecular weight estimation.
Product #’s PI84785, PI84786 Product # PI26635
Product # PI26610Product # PI26612Product # PI26614 Product # PI26630 Product # PI26632
Product #’s PI26616, PI26617 Product #’s PI26619, PI26620 Product #’s PI26623, PI26634 Product # PI26625Product # PI26628
BN03
3014
1 0
4/14
Prin
ted
in th
e U.
S.
thermoscientific.com/pierce© 2014 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only. Ready Gel is a registered trademark of Bio-Rad Laboratories, Inc. Strep-Tactin is a registered trademark of IBA GmbH. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
In the United States:For customer service, call 1-800-766-7000To fax an order, use 1-800-926-1166To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437To fax an order, use 1-800-463-2996To order online: www.fishersci.ca
© 2013 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries.