Download - Microb FKM Ver.1.0
-
8/12/2019 Microb FKM Ver.1.0
1/32
DEPARTMENT OF MICROBIOLOGYFACULTY OF MEDICINE HASANUDDIN UNIVERSITY
2014
Bacteria:Characteristics Identification
A.R. Sultan, MD., DMM., MScI&I
FK- Unhas
Makassar
-
8/12/2019 Microb FKM Ver.1.0
2/32
Familiar with these?
-
8/12/2019 Microb FKM Ver.1.0
3/32
-
8/12/2019 Microb FKM Ver.1.0
4/32
Bacterium
Microscopic, single-celled organisms belonging to Kingdom Monera that possess a
prokaryotic type of cell structure, which means their cells are non-compartmentalized, and their DNA (usually circular) can be found throughout
the cytoplasm rather than within a membrane-bound nucleus. They reproduce
by fission or by forming spores
Bacteria constitute a large domain of prokaryotic microorganisms. Typically a
few mm in length, bacteria have a number of shapes, ranging from spheres to rodsand spirals
-
8/12/2019 Microb FKM Ver.1.0
5/32
Morphology
Coccus Bacillus
-
8/12/2019 Microb FKM Ver.1.0
6/32
-
8/12/2019 Microb FKM Ver.1.0
7/32
Bacteria Cell Structure
-
8/12/2019 Microb FKM Ver.1.0
8/32
Bacterial Cell wall
-
8/12/2019 Microb FKM Ver.1.0
9/32
Virulence factors
-
8/12/2019 Microb FKM Ver.1.0
10/32
Exotoxin vs Endotoxin
-
8/12/2019 Microb FKM Ver.1.0
11/32
Pathogenesis in general
-
8/12/2019 Microb FKM Ver.1.0
12/32
ACCURATEMICROBIOLOGY DIAGNOSIS
SPECIMENS METHODS
INSTRUMENTS
/REAGENTS
HUMAN
RESOURCES
-
8/12/2019 Microb FKM Ver.1.0
13/32
Specimens:
Body fluid/tissue
Blood / LCS
Faeces
Urine
Mucosal swab
Diagnosis:
Direct-Gram preparation
Isolation / Culture
Antibiotic sensitivity tes
Serology and sero-typing testing
Molecular identifications
Bacterial Identification(In general)
-
8/12/2019 Microb FKM Ver.1.0
14/32
SPECIMEN
BACTERIA MACROMOLECULE
Bottle/med.
transport
Direct
Preparation
Isolation & IdentificationMicroscopy
Ag AbDNA OR
RNA
Bacterial Identification(In general)
-
8/12/2019 Microb FKM Ver.1.0
15/32
SPECIMEN
DIRECT SMEAR
WET MOUNT STAINING
FLUORESCENCEACID FASTG R A M
A. Direct examinations
-
8/12/2019 Microb FKM Ver.1.0
16/32
Light microscopy:
Wet mount:
Morphology
Motility
Preparation
a. Gram stain: morphology, Gram positive ornegative
b. Acid fast stain: morphology, Acid fast bacilli
c. Fluorescence: Increasing sensitivity of Acid fastpreparation.
A. Direct examinations
-
8/12/2019 Microb FKM Ver.1.0
17/32
Gram staining differentiates bacteria by the chemical and physical
properties of their cell walls by detecting peptidoglycan
-
8/12/2019 Microb FKM Ver.1.0
18/32
Gram staining
-
8/12/2019 Microb FKM Ver.1.0
19/32
-
8/12/2019 Microb FKM Ver.1.0
20/32
Gram Positive
Gram negative
Gram (+) versus Gram (-)
-
8/12/2019 Microb FKM Ver.1.0
21/32
Acid fast staining
Acid-fastness is a physical property of certain bacteria (such as M.
tuberculosis) specifically their resistance to decolorization by acids
during staining procedures
-
8/12/2019 Microb FKM Ver.1.0
22/32
Acid fast staining
B C lt d I l ti
-
8/12/2019 Microb FKM Ver.1.0
23/32
SPECIMEN
ENRICHMENT MEDIUM
BOTTLE OR TRANSPORT MEDIUM
SELECTIVE/DIFFERENTIAL MEDIUM
PURE CULTURE
Identi f ication GRAM BIOCHEM TESTS DNA Tes RAG
B. Culture and Isolation
-
8/12/2019 Microb FKM Ver.1.0
24/32
Colony appearances
-
8/12/2019 Microb FKM Ver.1.0
25/32
Colony appearances
-
8/12/2019 Microb FKM Ver.1.0
26/32
1. Form and margin
2. Sizes
3. Elevation
4. Clarity
5. Color : pigment,chemical reaction
6. Hemolysis : , , &
Colony appearances
-
8/12/2019 Microb FKM Ver.1.0
27/32
C. Biochemical Characteristics
-
8/12/2019 Microb FKM Ver.1.0
28/32
-
8/12/2019 Microb FKM Ver.1.0
29/32
1. Diffusion Test:
a) Disc diffusion test
2. Dilution test:
a) Tube dilution test
b) Micro-broth Panel dilution
Test
3. Combine :
a) E Susceptibility test
D. Antibiotic Sensitivity Test
-
8/12/2019 Microb FKM Ver.1.0
30/32
Disc Diffusion test
-
8/12/2019 Microb FKM Ver.1.0
31/32
-
8/12/2019 Microb FKM Ver.1.0
32/32
Thank youany Questions