Download - Praktikum Mikroskop
Praktikum Mikroskop
Peni KS Mutalib
Goals
• Being skilled to use microscope
• To know some important principle so that student could operate microscope: good in short time.
• Determine alternatif procedure which be wanted
• Making adaptor self and taking photo (optional)
Assignment in Practice Microscope January 2008
1. Mention the parts of a microscope!2. What is the function of those parts?3. How to optimalize the functions?4. What should be done to maximize your RP?5. What is the NA of your microscope?6. And from your right and left hand side microscope? 7. RP of no. 5 and 6: what is better? What is your argument?8. Figure the tissue with low, medium and high power
(magnification of objective 10x, 40x, and 100x)! Write the overall magnification 100x, 400x, 1000x.
9. Report your experiences in using microscope: The steps!10.What is the difference with the steps mention in theory?
Micoscope Figure
• Oculer: 10x, 15x, 20x• Objektive 10x, 40 x, 100x dan NA 0,45,
0,65• Condensor • Light source or mirror• Immersion oil• Magnification• Object glass
Optical Pathways (Magnifying)
Several theory
• Resolving Power
• NA: Numerical Aperture
• Immersion oil
• How to use Condensor
Lens protection and putting slide while
• Bringing microscope
• Cleaning lens
• Focusing small objective power 4x and medium 10x
• Studying slides with high power 40-45x or when using immersion oil
• Rapikan mik setelah selesai !
Identify part of the microscope• Do it several times, think about the
function
Different location of coarse and fine adjustment
Fine ajustment
How to get the best RP from a microscope incl. how and when to
do:
• Cleaning the lenses
• Illuminate the specimen
• Focusing the condenser
• Ajust the diagfragma
• Using immersion
• Setting light intensity
Ocular and objective lens
Magnification & Numeric Aperture
NA = n sin
Resolving Power
Resolution of lens camera is revealed by how many lines per inch (lpi)
Lens resolution• Is the ability of a lens to free shadow from
2 point of thing that is very close.• Resolusion limit = 1.22/D (Resolusion depend
on )
• Daya urai (=Resolving Power;RP;s)= f= 1.22f/D (D=2sin) where f=jarak objek ke lensa objektif
How smaller how smaller RP
How larger D how smallerRP
How bigger how smaller RP
Light source
Long wave length have greater energy
Electromagnetic wave spectrum
Step in focusing microscope
Step
Step
The best RP visual microscope +- 200 nm
Magnification is not the same with RP
RP microscope mainly depend on obj lens (oculer-max 10x, only magnify image which is found from obj lens-max 100x). Oculer lens didn’t make better the resolusion. Higher magnification with low resolution image blur which is < function
NA= n sin
RP=K.
NA
L objOn the objective was wrote Plan 25/0.45 (Magnification/NA) 160/0.17Tube length in mm
Thickness(in mm) deck glass for which obj lens is corected (dry field)
RP = 0,61 NA
=(0,61)(5,50 x 10-7 m
1,62 x 10 –7 m 200
nmn=1,8 (n immersion oil)
sin 0,90
Large (Fast) NA vs. Small (slow) NA
Thus, NA:
Condensor
• Make better RP with making light lamp 100% of the image-forming kind
• How: with focusing light in the shape of inverse cone, with apex at the specimen and base at the objective
Modern Research Microscope
Tempo, 22 Juni 2003: hal. 16
NA 1.40
CCD camera US$3500
MPixel ?