Download - Prinsip Kerja MGIT
•Mycobacteria Growth Indicator Tube• Modified Middlebrook 7H9 broth base• Detection of growth through detection of O2
consumption• Fluorescent sensor responds to O2 concentration• Mycobacteria are aerobic organisms and will utilize
O2 at readily detectable rates
WHAT IS MGIT ?
• Menggunakan MGIT media dan patented sensors, menjadikan lebih efisien dengan penggunaan teknologi fluorometri.• Deteksi yang akurat pada
pertumbuhan bakteri dengan cara mendeteksi konsumsi O2 oleh bakteri.
• Automated instrument quality control• Performed continuously to ensure
precise and reliable operation.• Specimen results provided as
Positive or Negative.
BACTEC® MGIT™ 960
MGIT Technology indicator system
tris-4, 7-diphenyl-1, 10-phenanthroline ruthenium dichloride pentahydrate-DPPRDP
Negative Culture
CO2
O2O2
O2 O2
O2
O2
Vial Headspace
Growth Medium
Sensor
Little or No Fluorescence
FF F
FFO2
FO2
F
F
F F
CO2
O2
O2
O2
VialHeadspace
Growth Medium
Sensor
Positive Culture
Strong Fluorescence
FO2FO2
FO2
FO2FO2
F
F
CO2
CO2
CO2
105-106 CFU/ml
10% CO2
• Detector Board located in each drawer.• 16 individual detection
stations.• Data collected from each tube
once each hour.• Data evaluated and tube
positivity established based on software algorithms.
DETECTION SYSTEM
• Indicator lamp on drawer illuminates.• Audible alert sounds.
NOTIFICATION OF POSITIVES