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Production of clean seed

The virus-free program

at WRPIS (Pullman, WA)

1) History

2) The “TEST”

3) The “clean-up”

4) The future

History

• 1983 >70% infected w/ BCMV

• 1985 original protocol

• 1994 leaf tissue testing

• 23.3% of collection virus-free

The “TEST”

• Tissue harvest

• ELISA testing

• Clean

• “Dirty”

The “clean up”

• <10% BCMV infection

– rouging

• >10% BCMV infection

– single planting, harvest & testing

• Re-combining clean seed

¿ The future ?

• Widely recognized protocol

• Adapted by other germplasm collections

• Virus testing technician

• Part-time help? Soft money?

• Suggestions?????