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Protein Electrophoresis
By: Dr. Beenish Zaki
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Introduction to Electrophoresis
Electrophoresis is a laboratory technique where the blood serum (is placed on agarose gel and exposed to an electrical current to separate the serum protein components.
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Normal Pattern
Note the relative sizes and shapes of the globulin fractions.
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EQUIPMENT FOR ELECTROPHORESIS
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ELECTROPHORETIC PACK
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POWER PACK
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AGAROSE GEL
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Micropipette and Applicator
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DRYER
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INTERPRETATION OF ELECTROPHORESIS
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Cirrhotic Pattern
In the cirrhotic pattern, the distinction between beta and gamma globulin is blurred and is sometimes referred to as the "beta-gamma bridge" pattern.
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Monoclonal (M) Protein Present
Serum shows the M protein was IgG kappa.