S6 S7-2S9-1

S10Mock

Agro-infiltrated leaves Systemic leaves

Marker

PVXS7-1

S8 S9-2S6 S7-2

S9-1S10

MockMark

er

PVXS7-1

S8 S9-2

PVX CPantiserium

loading

34 kDa26 kDa

Systemic leaves

S6 S7-2S9-1

S10Mark

er

PVXS7-1

S8 S9-2

PVX CPantiseriumloading

34 kDa

Fig. S2

A

B

PVX

PVX-S8

PVX-S10PVX-S9-2

PVX-S9-1

PVX-S7-2PVX-S7-1

PVX-S6

N. Benthamiana leaves infiltrated with chimaeric PVX vectors ( 7 dpi)

PVX PVX-S7-2PVX-S7-1PVX-S6

PVX-S8 PVX-S10PVX-S9-2PVX-S9-1

N. Benthamiana leaves infiltrated with chimaeric PVX vectors (15 dpi)