![Page 1: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/1.jpg)
TITLE SLIDE GOES HERE “"So many genomes, so little time:
the future of plant breeding"”
149 (2000))
David Francis (francis.77”at”osu.edu)
![Page 2: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/2.jpg)
![Page 3: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/3.jpg)
DG = k* σP* h2 Everything you need to know about breeding is in this equation
• The importance of genetic variation (h2 = σG /σP) • K, σP, h
2 are subject to disruptive technologies which may improve efficiency* of selection (or may just cost a lot of money).
• Larger populations improve measurement of σG
*Efficiency DG Cost Time
![Page 4: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/4.jpg)
"Those that fail to learn from history, are doomed to repeat it." Winston Churchill
"We learn from history that we learn nothing from history." George Bernard Shaw
![Page 5: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/5.jpg)
S. pimpinellifolium, S. galapagense (plant form) S. pennellii, S. peruvianum, S. pimpinellifolium (resistance)
as source of σG
Plant breeding has always been more effective when coupled to new technology, whether that technology is production, harvesting, or breeding (e.g. development of mechanically harvested tomato)
![Page 6: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/6.jpg)
Most of our disease resistance has come from wild species σG
![Page 7: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/7.jpg)
Many of the differences between market classes are due to genes
from wild species σG
![Page 8: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/8.jpg)
Take home messages: • NGS sequence data is providing a resource for
allele identification, but… • Lack Reference (elite material) • Lack structure-function information
• Fastest way to evaluate new alleles is breeding • CRISPR/CAS9 is the newest mutation breeding
tool (loss of function will be more common) • GS models show promise for prediction of
performance • Large populations and biological assessment
Knowledge of traits and trait relationships
![Page 9: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/9.jpg)
Since October 2014 We have ~500 tomato genomes in public databases: how can we use this information?
Tomato Genome Consortium - 2012 Resequencing projects 2014 (Reference sequence)
![Page 10: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/10.jpg)
![Page 11: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/11.jpg)
Cluster of tm-2 alleles from of 85 unique genomes in “150 genome” project
![Page 12: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/12.jpg)
S R
Cluster of tm-2 alleles from of 85 unique genomes in “150 genome” project + 2 resistance alleles Tm-2 and Tm-2a
(let’s sequence relevant germplasm…)
![Page 13: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/13.jpg)
Susc.
Res.
• Evaluation of accessions for Tm2 DNA Sequence, virus symptoms, and antibody reactivity.
• Resistant phenotypes found on most nodes of accession tree Susceptible phenotypes found on most nodes of accession tree.
• Only predictability when we all ready know the allele. • We lack sufficient structure-function information to predict new
resistance alleles.
![Page 14: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/14.jpg)
Creation of new genetic variation “Gene Editing” technologies 1) Zinc finger nucleases 2) Transcription activator-like nucleases (TALENs) 3) Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9(Cas9) endonuclease 1 and 2 are based on protein-DNA interactions, 3 is an RNA-guided DNA endonuclease system
![Page 15: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/15.jpg)
Image from: Horvath P, Barrangou R (2010). "CRISPR/Cas, the immune system of bacteria and archaea". Science 327 (5962): 167–170.
To alter a target gene, supply Cas9 and the guide RNA Easier to knock out (remove function) than to create a new function – there are exceptions to this rule
![Page 16: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/16.jpg)
Pool Bait Capture Sequence
Image from: Gnirke A, Melnikov A, Maguire J, et al. Solution Hybrid Selection with Ultra-long Oligonucleotides for Massively Parallel Targeted Sequencing. Nature biotechnology. 2009;27(2):182-189
How do we identify targets?
![Page 17: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/17.jpg)
![Page 18: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/18.jpg)
Example: T3 resistance
from PI128216
IBC population
SSR6370.9 SL10915 TOM144
TOM1963.0SL10737i
8.4
SL10615i
4.6
CosOH57
3.9
SL201813.3
Rx-4
18.8
SL10027
Robbins et al., 2009.
Phytopathology
4 markers in 30 cM on
chromosome 11
![Page 19: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/19.jpg)
CC-NBS-LRR resistance gene
Potential targets based on candidate gene identification
![Page 20: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/20.jpg)
Solyc11g069020 = XV3/Rx4
CC-NBS-LRR resistance gene
![Page 21: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/21.jpg)
I-2 (Tomato) RPS2 (Ath) BS2 (Pepper) 240 reannotated NB-LRR genes were used together with 30 functionally characterized plant R genes. Expressed genes (cDNA).
![Page 22: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/22.jpg)
Yield Analysis and Prediction
![Page 23: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/23.jpg)
Why is David excited about GS and prediction?
Long-term Goal: Learn how to make a better hybrid • Evaluate a partial diallele with 96 hybrids and
parents (2 years, 2 locations, 2 reps/location RBC) • Some evidence for hybrid vigor (hybrids preform
~5-10% better than parents) • No evidence for heterotic groups
This is Matt Robbins
![Page 24: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/24.jpg)
Best prediction of hybrid performance was parent performance (good parents make good hybrids)
![Page 25: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/25.jpg)
Diversity in tomato populations (processing)
California Ohio Undeterimined Ridgetown College, Ontario Oregon
CA H O
Clustering based on 7,700 SNPs
![Page 26: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/26.jpg)
Genome Wide Selection (Meuwissen T.H., Hayes B.J., Goddard M.E.
(2001) Prediction of total genetic value using genome-wide dense marker maps. Genetics, 157, 1819–1829.)
• Detection of sequence variation is no longer limiting • Promise of predictive models
𝜸 = µ + Ẍ 𝛽 + ∈ 𝜸 is the vector of phenotypic values for n individuals Ẍ is the n x K marker matrix for K markers 𝛽 is the estimated breeding value associated with each marker
Goal: Predict the performance of an individual based on genotype
![Page 27: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/27.jpg)
Phenotype Data Distributions ANOVA Partitioning Variation (heritability) BLUPs
Structure Q Matrix (PCA)
Genotype Data Marker Matrix
Association Analysis to establish marker-trait linkage (Fixed effect) Estimate Breeding Value (Random effect)
Kinship matrix
![Page 28: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/28.jpg)
Genomic Selection (GS) is exciting because it promises the ability to predict performance based on sequence variation (C/T).
![Page 29: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/29.jpg)
How prediction models work:
DNA extracted and allele specific assays conducted to determine genotypes.
The result is a matrix of marker data coded based on allele dose.
Selections made based on “model” (developed from previous experience with performance and sequence information).
![Page 30: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/30.jpg)
X1[1:5, 1:8] M1 M2 M3 M4 M5 M6 M7 M8
1 1 0 0 1 -1 0 0 1
2 0 0 0 -1 1 0 1 1
3 1 0 0 1 -1 0 1 1
4 1 1 1 0 0 0 0 1
5 0 -1 -1 0 0 0 0 1
[96 x 384] x [384 1] = [96 1] [G x M } x [M] = Prediction for 96 Genotypes
X
Keep those at K = 1 (~15% of the population)
![Page 31: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/31.jpg)
SolCAP Collection 140 inbred lines evaluated in CA and OH (2 year, 2 locations RBC design) 288 Nested RIL progeny (OH2641 x OH987034; OH7814 x OH987034; OH2641 x OH981136) (O x H; O x H, O x CA)
Augmented Experimental design (2 year, 2 locations)
![Page 32: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/32.jpg)
Populations (SolCAP) and (OxH; OxH, OxCA)
California Ohio Undeterimined Ridgetown College, Ontario Oregon
CA H O
Clustering based on 7,700 SNPs
![Page 33: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/33.jpg)
Traits: Total traits measured: 52 Yield, digital phenotyping and chemical meas. Reduced to 22 most informative (h2, PCA and other methods) • Yield (total and marketable) • Color and Color uniformity • BRIX • pH • Vitamin C • Fruit Size and Shape
0.40
0.45
0.50
0.55
0.60
0.65
0.70
0.75
0.80
0 2 4 6 8 10Pro
p. N
o. 1 t
om
ato
es
Hue uniformity
Proportion No. 1 tomatoes VS Hue uniformity
![Page 34: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/34.jpg)
![Page 35: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/35.jpg)
Predicting inbred-line performance from inbred line data Data from SolCAP data (140 varieties, 7,700 markers)
Yield
Fruit Size
![Page 36: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/36.jpg)
Predicting inbred-line performance from inbred line data Results from nested RIL (280 x 384 markers)
Yield
Fruit Size
![Page 37: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/37.jpg)
Predict hybrid performance from inbred data Training Populations (Genotype and Phenotype) SolCAP (inbreds) Nested RIL (inbreds) Hybrids Predict genotype from inbred data Predict performance using GW model(s) developed for inbreds Compare prediction with actual performance
![Page 38: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/38.jpg)
[384 1] x [96 384] = [96 1] M x [G x M]
Model: Vector of Marker Estimated Breeding Value (Contribution of each marker to trait)
M1 M2 M3 M4 M5
Hybrid genotype matrix (estimated from inbred genotypes)
![Page 39: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/39.jpg)
[384 1] x [96 384] = [96 1] M x [G x M] = Prediction for 96 Hybrids
Model: Vector of Marker Estimated Breeding Value (Contribution of each marker to trait)
Hybrid genotype matrix (estimated from inbred genotypes)
M1 M2 M3 M4 M5
![Page 40: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/40.jpg)
How does predicted performance relate to actual performance?
![Page 41: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/41.jpg)
Predicting Hybrid Performance from inbred-line data
Model from SolCAP (n =140 K = 7,700) from RIL (n = 280 K = 384)
Ripe_kg_(vs)_MktYield P = 4.42E-05 R2 = 0.19958
Yield_(vs)_Yield P = 0.001182 R2 = 0.126445
![Page 42: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/42.jpg)
Association analysis a = Yield as BLUP b = Yield normalized c = fruit weight d = fruit number e = vine size f = maturity
![Page 43: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/43.jpg)
Currently: Testing to see if additive models can be improved by incorporating non-additive effects
+ = better
prediction?
![Page 44: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/44.jpg)
Ability to predict performance based on genetics is an exciting development, but "beyond mountains there are mountains“.
• What are we selecting for (𝜸) 𝜸 = µ + Ẍ 𝛽 + ∈ ?
• Yield, Brix, Color, Viscosity, Resistance (n = 5-17)
• GWS for multiple traits requires that we have robust Multi Trait Indices (MTI) for selection
![Page 45: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/45.jpg)
Trait weights
![Page 46: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/46.jpg)
Trait Weight Yield +2 Color -1 Brix 0 etc…
![Page 47: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/47.jpg)
Traits: What can historical data tell us about trait models? California PTAB
• 20 years • 585 varieties • 8.8 million of loads
Farm Advisor Variety Trials • 10 years • 173 varieties
Traits Measured – Yield – Brix – pH – Color – Defects – Number of loads produced
• Can we predict performance in market with traits value?
![Page 48: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/48.jpg)
V = Multi-Trait Index weighting Yield, BRIX, Color and limited use. Evaluated based on ability to predict market success (% load and longevity) in varieties from PTAB and UCCE year data set.
![Page 49: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/49.jpg)
Conclusions: Response to challenges can be faster 1) Genetic resources remain a foundation; 2) Sequence data in public data bases allows rapid evaluation of new alleles for function, the best way to do this is still through breeding (Eka’s talk); 3) Whole genome models have predictive capability for individual performance and hybrid performance; prior knowledge of QTL position helps (Debora’s talk); 4) Population size is more important than marker number; 5) Knowledge of selection targets will be key to success.
![Page 50: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/50.jpg)
Thank you for your time. Questions?
![Page 51: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/51.jpg)
Some up coming meetings
15-18 August, Raleigh NC
![Page 52: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/52.jpg)
Shameless advertisement:
All 3 = HCS 8825 Individual workshops as HCS 8806 (Methods)
![Page 53: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/53.jpg)
Reminder: Support germplasm center(s) http://tgrc.ucdavis.edu/Donate/
Ph
oto
cre
dit
s: S
ilvia
Fra
nci
s,
Ch
arle
s R
ick
Ph
oto
credits: R
oger C
hetelat, TG
RC
![Page 54: So many genomes, so little time: TITLE SLIDE GOES HERE the ...tgc.ifas.ufl.edu/2016/2. So many genomes, so little... · Hipolito Medrano Pep Cifre Josefina Bota Miquel Angel Conesa](https://reader033.vdocuments.net/reader033/viewer/2022052723/5f0e670d7e708231d43f12e1/html5/thumbnails/54.jpg)
Acknowledgments Collaborators, OSU
Debora Liabeuf
Eka Sari
Eduardo Bernal
Michael Dzakovich
Marcela Carvalho Andrade
Regis de Castro Carvalho
Troy Aldrich
Jihuen Sim
Caleb Orchard
Gabriel Abud
Elisabet Gas Pascal
Heather Merk
Sung-Chur Sim
Matt Robbins
Steve Schwartz
Rachel Kopec
Jessica Cooperstone
Luis Rodrigues-Saona
Sally Miller
Collaborators, CAU Wencai Yang
Hui Wang
Collaborators, INRA CeMathilde Causse
Collaborators, UIB Hipolito Medrano
Pep Cifre
Josefina Bota
Miquel Angel Conesa
Collaborators,
Industry Cindy Lawley, Illumina
Martin Ganal, Trait
Genetics
Hirzel Canning
Red Gold Canning
Collaborators, Cornell Walter de Jong
Lucas Mueller
Martha Mutschler
Collaborators, UCD Allen Van Deynze
Kevin Stoffel
Collaborators, MSU David Douches
C Robin Buell
John Hamilton
Dan Zarka
Kelly Zarka
Collaborators, UFL Sam Hutton
Jay Scott