Download - Solexa tag analysis
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Solexa tag analysis
0620
ahsan
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今日の結果• 発現量の比較結果
– Upload 済み• 遺伝子の名前で keyword 検索できる• Distance20 かつ tag40 以上 cluster を作
りました。– Cluster の代表 TSS 決まる core
promoter 領域の motif 解析– Core promoter motif の module の存在– Annotation TSS から代表 TSS の距離– Annotation TSS から cluster 開始点の距離
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Tag to Gene Relation
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Keyword 検索
URL: http://dev.utgenome.org/~utgb/drosophila/genome_browser/browser.php
Keyword: 遺伝子名前を記入するKeykinds: geneName を選択する
FBtr0083733 sqz-RAFBtr0084012 ppan-RAFBtr0078728 Xe7-RAFBtr0078729 Xe7-RBFBtr0081642 Aly-RAFBtr0076616 Gug-RA
FlybaseTrID GeneName
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DNA Replication Element Binding Factor
Image1 :Core promoter. Transcription Start Site (TSS) position is 0, where TATA at -30 to -25 , DPE at +29 to +33, MTE at downstream. DRE position is not restricted by upstream of TSS.
DRE TATA Inr DPE
Initiator Downstream Promoter Element
TATA Box
MTE
Motif Ten Element
Core promoter Structure
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MotifChromosom
alCore
promoter
DRE 23,792 527
TATA 31,629 715
INR 52,111 371
DPE 29,781 455
MTE 45,196 547
motif element in Drosophila Chromosome and RepTSS core promoter
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TATA motif position in ResTSS core promoter
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INR motif position in ResTSS core promoter B
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DPE motif position in ResTSS core promoter C
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MTE motif position in ResTSS core promoter D
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(TATA,INR,DPE,MTE) RepTSS #
0000 18,600
0001 505
0010 403
0100 320
1000 652
1100 22
1010 21
1001 18
0110 18
0101 10
0011 12
0111 0
1011 1
1110 0
1101 1
1111 0
co-existence of binding motif in core promoter region
Here 0,1 express, the present and absent of each motif elements in written order
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TSS distance
Larva(distance from TSS to RepTSS)
0
200
400
600
800
1000
-1500 -1000 -500 0 500 1000 1500
distance
num
ber
of T
rans
crip
t
AllTag
0
1000
2000
3000
4000
5000
6000
-1000 -500 0 500 1000 1500 2000 2500 3000
cluster StartPoint distance from annotationTSSTr
ansc
riptio
n N
umbe
r
Annotation TSS から代表 TSS の距離 Annotation TSS から Cluster の開始点の距離