Biotechnological development of Aptamers
Dr. Sonia Da Rocha Gomes
Emilie Daguerre
Laetitia Evadé-Farrugia
Novaptech,
146 rue Léo Saignat, bat 3A, BP12
Bordeaux, France
+33.5.57.57.95.05
10th April 2014, Human Health Track, Barcelona
Novaptech, business unit
-Created in 2008,
-3 people, Bordeaux
-Dedicated to the biotechnological development of APTAMERS
-Managed by the association
-Connected to the academic lab Inserm U869, Dr. Toulmé ’s team > 15 years of expertise in
aptamer field (11th, Agrofood session)
- Two automated platforms of selection and aptamer production, unique in France
Aptamer production
- Identification - Optimization - Conjugation
Aptamer-based tools
in analytical, diagnostic
or therapeutic fields
Method development
-Implementation of new strategies
-Improvement of the automated platforms
OFFER :
Aptamer selection against your target and
characterization
Service contracts with academic labs and
companieS
30 projects
Your needs, looking for new molecular probe
DETECTION AND MONITORING (molecular imaging,
biosensors, sandwich assay)
PURIFICATION (affinity capture, sorting)
CHARACTERIZATION (target validation,
protein-binding RNA sites)
INHIBITION (drug delivery, drug discovery) REGULATION
(artificial riboswitch)
Aptamers, the right solution
Aptamer properties,
Structured oligonucleotides identified:
from randomly synthesized library
against a target
through combinatorial strategy (SELEX)
iterative rounds of selection / amplification
(Tuerk and Gold,1990; Ellington and Szostak, 1990)
High affinity and specificity
Rival antibodies
Chemical synthesis
Chemically modified and
conjugated to numerous pending
groups (fluorophore,
radioelements, biotin..)
Aptamer selection process,
146 rue Léo Saignat, bat 3A, BP12 Bordeaux, France
[email protected] +33.5.57.57.95.05
Phase 1
Phase 2
Automated platform for aptamer selection, unique in France (Inserm U869 lab)
Aptamer
Selection
Screening
(Affinity)
Cloning
Sequencing, Sequence analysis
Candidates production and
binding validation
Optimization
- Minimizing their size
- Improving nuclease resistance
- Conjugation: biotine, amine, fluorochrome..
